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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Yu-Shin Chen | en |
| dc.contributor.author | 陳瑜歆 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:42Z | - |
| dc.date.available | 2021-07-01T08:15:42Z | - |
| dc.date.issued | 1991 | |
| dc.identifier.citation | (1) F. Sanger, A.R. Coulson, B. G. Barell, A. J. H. Smith, and B. A. Roe, J. Mol. Biol. 143, 161 (1980) (2) A. Maxam, W. Gilbert, Meth. Enzymol. 65 : 499 - 560 (1980) (3) J. G. Sutcliffe, Cold Spring Harbor Symp. Quant. Biol. 43, 77 (1979) (4) S. Anderson, Nucleic Acids Res. 9, 3015 (1981) (5) J. Messing, R. Crea, and P.H. Seeburg, Nucleic Acids Res. 9, 309 (1981) (6) A. M. Frischauf, H. Garoff, and H. L.ehrach, Nucleic Acids Res. 11, 1645 (1983) (7) G. F. Hong, I. Mol. Biol. 158, 539 (1982) (8) M. Poncz, D. Solowiecjczyk, M.Ballantine, E. Schwartz, and S. Surrey, Proc. Natl. Acad. Sci. U. S. A. 79, 4298 (1982) (9) W. M. Namels, M. Bevan, and P. H. Son, Meth. Enzymol. Vol. 101, p. 98 (10) J. L. Hartley and J. E. Donelson, Meth. Enzymol. Vol. 100, p. 116 (11) S. Heikoff, Gene 28, 351 (1984) (12) R. M. K. Dale, D. A. McClure, and J. P. Houchins, Plasmid 13, 31(1985) (13) C. Yanisch-Perron, J. Vileira, and J. Messing, Gene 33, 103 (1985) (14) T. K. Misra, Gene 34, 263 (1985) (15) A. T. Bankier, and B. G. Barell, Techniques in Life Sciences, B5:Nucleic Acid Biochemistry p. 1 - 34 (1983) (16) L. H. Guo and R. Wu, Nucleic Acids Res. 10, 2065 (1982) (17) K. D. Tartof, and C. A. Hobbs, BRL Focus 9, 12(1987) (18) A. M. Frischauf, H. Lehrach, A. Poustka, and N. Murray, J. Mol. Biol. 170, 827 - 842 (1983) (19) T. J. Sithary, M. L. Berman, and L. W. Enquist, Experiments with Gene Fusions, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1984) (20) T. Maniatis, E. F. Fritsch, and J. Sambrook, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982) (21) M. G. Murray, and W. F. Thompson, Nucleic Acids Res. 8,4321 - 4326 (1986) (22) K. Kaisen, and N. Marray, In DNA Cloning techniques : A Practical Approach (1985) (23) E. Y. Chen, and P. H. Seeburg, DNA 4: 165 - 170 (1985) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75827 | - |
| dc.description.abstract | 利用二種 restriction endonuclease : Rsa I，Taq I互相配合，在不同的反應時間控制下可以把載體為 pUC19 的 recombinant DNA 做同一方向（ unidirectional ）切割成大小不同的subfragments ，再經由 self - ligation , transformation 之後，可得到一 ladder 長短不同的 DNA f ragments （附圖五），以這個方法配合 double strand DNA sequencing method ( 23 )決定了一級鯉魚 r - crystallin 基因 ( 1 . 8 kb ) . | zh_TW |
| dc.description.abstract | We used two different kinds of restriction endonucleases, Rsa I and Taq I, to digest a large DNA fragment that is inserted to pUC 19 vector in a unidirectional deletion manner. Through a time course (about 1 - 5 minutes), the recombinant DNA became different sizes of subfragments. Appropriate size of those deleted subclones were checked and picked out after transformation and mini preparation. These subclones together with the use of dideoxy chain termination sequencing method made an insert DNA with size 1 - 6 kb could be sequenced in a short period. We have exploited this strategy to decide the sequence of carp egg genome, and found out several γ- crystallin genes. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:42Z (GMT). No. of bitstreams: 0 Previous issue date: 1991 | en |
| dc.description.tableofcontents | 中文摘要-----------------------------------------------------2 英文摘要-----------------------------------------------------3 緒言---------------------------------------------------------4 - 5 材料與方法---------------------------------------------------6 - 16 結果---------------------------------------------------------17 – 19 討論---------------------------------------------------------20 – 22 附圖---------------------------------------------------------23 – 30 參考文獻-----------------------------------------------------31 – 32 謝誌 ---------------------------------------------------- 33 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用限制酵素在 pUC載體作 DNA序列 之同向定序 | zh_TW |
| dc.title | ‘ Unidirection ’ deletion of DNA strectch for sequencing with Rsa I / Taq I endonuclease on pUC vector | en |
| dc.date.schoolyear | 79-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 35 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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