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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75812完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 宋延齡 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:35Z | - |
| dc.date.available | 2021-07-01T08:15:35Z | - |
| dc.date.issued | 1975 | |
| dc.identifier.citation | (1) Anderson Douglas P. & Avron J. Ross (1972). Comparative study of Hagerman Redmouth disease oral bacterins. Pro. Fish-Culturist, 34(4), 226-228.
(2) Anderson Douglas P. & John R. Nelson (1974). Comparison of protection in Rainbow Trout (Salmo gairdneri) inoculated with and fed Hagerman Redmouth bacterins. J. Fish. Res. Board. Can. 31(2), 214-216. (3) Andrews P. (1964). Estimation of the molecular weights of proteins by Sephadex gel filtration. Biochem. J. 91, 222-233. (4) Bab?s V. & P. Riegler (1903). Zentra 1b. f. Bakt., Parasit.u. Infek., Abt. I. Orig., 33, 438-449. (5) Breed R.S., E.G.M. Murray & N.R. Smith (1957). Bergey's Manual of Determinative Bacteriology, 7th ed. 1094pp. (6) Donald F. Amend & Avron J. Ross (1970). Experimental control of Columnaris disease with a new Nitrofuran drug P-7138. Pro. Fish-Culturist, 32(1), 19-25. (7) Fryer J.L., J.S. Nelson & R.L. Garrison (1970). Vibriosis disease of fish. Oregon State Game Commission, Portland, Oregon; Agricultural Experiment Station Technical Paper No.2879, 23. Corvallis, Oregon. (8) Hoshina R. (1962). J. Tokyo Univ. Fisheries (Special ed.), 6, 1-105. (9) Isbell Gary L. & Gilbert B. Pauley (1974). The immune response of the Brown Bullhead (Ictalurus nebulosus) to A. liquefaciens & characterization of the resulting antibodies. Res. Fish. Con. No. 415, 46. (10) Kawatsu Hiroshi (1968). Immune response of fish. Bull. Jap. Soc. Sci. Fish. 34(3), 246-249. (AAser (1925)). (11) Kolntz G.W. & D. P. Anderson (1970). Oral immunization of salmonids. A symposium on diseases of fishes and shellfishes. p.16020. Edited by S.F. Snieszko. Spec. Publ. 5., Am. Fish. Soc., Wash., D.C. (12) Kou Guang-Hsiung (1973). Studies on the Pathogenecity of the fish pathogen, Aeromonas liquefaciens-Ⅲ. Variation of pathogenecity caused by inoculation into fish. J. Fish. Soc. Taiwan, 2(2), 16-19. (13) Liu P.V. (1961). Observations on the specificities of extracellular antigens of the Genera Aeromonas and Serratia. J. Gen. Microbial., 24, 145-153. (14) Lowry O.H., N.J. Rosebrough, A.L. Farr & Randall R.J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275. (15) Muroga Kiyokuni & Syuzo Egusa (1969). Immune response of the Japanese eel to Vibrio anguillarum-II. Possibility of oral immunization. Fish. Path., 4(1), 6-10. (16) Nybelin O. (1935). Ztschr. Immunit. Forsch., 84, 74-79. (17) Rose Noel R. & Pierluigi E. Bigazzi (1973). Methods in Immunodiagnosis. 2-3. (18) Scheidegger J.J. (1955). Une micromethode I' immunoelectrophoreses Internat. Arch. Allergy Appl. Immunol., 7, 103-110. (19) Shimizu Tomoko (1969). Studies on pathogenic properties of Aeromonas liquefaciens-Ⅲ. Some chemical and antigenic properties of toxic factors. (20) Spence K.D., J.L. Fryer & K.S. Pilcher (1965). Can. J. Microbiol., 11, 397-406. (21) Takahashi Yukinori & Riichi Kusuda (1971). Studies on the scale protrusion disease of carp fishes-Ⅱ. Immune response of carp to Aeromonas liquefaciens. Fish. Path., 6(1), 24-29. (22) Van Duijn C. Diseases of Fishes. 2nd ed. 81, London Iliffe Books. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75812 | - |
| dc.description.abstract | 1．Aeromonas hydrophila的弱毒性活菌、福馬林殺死菌、加熱死菌及聲波粹取物當做抗原，免疫鰻魚，都可以誘使抗體的產生。 2．四組抗原處理鰻魚後，皆在第四星期左右抗體效價達到最高，以後則迅速下降。高效價抗體存留的時間頗短。 3．四組抗原中以弱毒性活菌免疫鰻魚所產生的抗體效價最高，在第四星期抗體效價達到512。福馬林殺死菌免疫鰻魚後也在第四星期抗體效價達到最高，?256。加熱死菌則在第五星期達到最高，?128。至於聲波粹取物免疫鰻魚，抗體產生很低，在第四星期抗體效價達到最高，僅?64。 4．弱毒性活菌免疫鰻魚後的第四星期，以病原株做攻擊實驗，結果與對照組有顯著差異。顯示抗體對鰻魚的再次感染具有保護作用。 5．弱毒性活的經口服免疫鰻魚，2個月內都無法診測出凝集抗體。 6．Aeromonas hydrophila經聲波振破處理之粹取物，由Sephadex G-100分析得到兩個部份：A部分含6個成份，B部分含2個成份，其中一個成份重覆出現。推斷誘使抗體產生的大部分成份包含在A部分內。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:35Z (GMT). No. of bitstreams: 0 Previous issue date: 1975 | en |
| dc.description.tableofcontents | 一、緒論……………………………………………………1 二、材料與方法……………………………………………………3 免疫反應……………………………………………………3 ? 實驗動物……………………………………………………3 ? 細菌培養……………………………………………………3 ? 疫苗製造……………………………………………………3 ? 免疫過程……………………………………………………4 ? 凝集抗體效價的決定……………………………………………………5 ? 病原性的增強……………………………………………………6 ? 再攻擊實驗……………………………………………………6 抗原之初步分析……………………………………………………8 ? 粗抗原之製備……………………………………………………8 ? 抗體之製備……………………………………………………8 ? 粗抗原的分析……………………………………………………8 ? 抗原成份之比較……………………………………………………9 三、結果……………………………………………………11 四、討論……………………………………………………15 五、摘要……………………………………………………19 六、謝辭……………………………………………………29 七、參考文獻……………………………………………………30 | |
| dc.language.iso | zh-TW | |
| dc.title | Aeromonas hydrophila (A. Liquefaciens) 的免疫反應及抗原之初步分析 | zh_TW |
| dc.date.schoolyear | 63-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 32 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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