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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Fan-San Lin | en |
dc.contributor.author | 林奮生 | zh_TW |
dc.date.accessioned | 2021-07-01T08:15:25Z | - |
dc.date.available | 2021-07-01T08:15:25Z | - |
dc.date.issued | 1974 | |
dc.identifier.citation | Literature cited 1. Civcrolo E, I. and H. L. Keil. 1969. Inhibition of bacterial spot of peach foliage by Xanthomonas pruni bacteriophage. Phytopathology 59 : 1966—1967. 2. Denhardt ,D., and R • L. Sinshcimer. 1965. The process of infection with bacteriophage ?x174.V. Inactivation of the phage—bacteriun complex by decay of 32P incorporation in the infecting particle. J. Mol. Bid. 12 : 663—673. 3. Hohn B., H.Lechner, and D• A Marvin. 1971. Filarnen— tous bacteria virus. I. DNA synthesis during the early stages of infection with fd. J. Mol. Biol.56: 143—154 4. Hsu, Y. C. 1968. Regulation of the propagation of HR virus plaeimid. Cold Spring Harbor Symp. Quant. Biol 33: 367—471. 5. Hsu, Y. 0. 1968. Propagation or elimination of viral infection in the carrier cells. Bacteriol. Rev 32;387—399.. 6. Kuo, T. T. T. C. Huang, arid C. T. Chow. 1969. A filamentous bacteriophage from Xanthomonas oryzae. Virology 39: 548—555. 7. Kno, T. TA 11. C. Chang, C. U. Yang, and S. P. Yang.1971. Bacterial leeg blight of rice plant. IV. Effect of bacteriophage on the infectivity of Xanthoraonas oyzae. Pot. Bull. Academia Sinica 7: 1—9. 8. Kuo, T. T. T. C. Huang, R. Y. Wu, and C. M. Yang.1967. Characterizetion of three bacteriophages of Xanthomonas oryzee Bot. Bull.Academia Sircice. 8:246—254. 9. Ku.T. T.C. M. Yang, Y. Y. Yang, and S. P. Y. Hsieh.1968. The distribution of strains of Xanthomona oryzae and it page in Taiwan. Plant Prot. Bull. (Taiwan) 10:1—8. 10.Livhyihdyrin, J.M. D. Barner, and . S• S.Cohen. 1960. The metabolism of exoenous1y supplied nucleotides by E. coil. J. Biolo. Chem. 235: 457—465. 11. Marvin, D, A., 1968. Control of DNA replication by Membrane. Nature 219: 485—486. 12. Marvin, D, A., and B. Hohn. 1969. Filaimentous bacterial viruses. bacterial. Rev. 33: 172—209. 13. Okabe, N, and M. Goto. 1963. Bacteriophages of plant pathogen. Annu. Rev. Phytopathology 1: 397—418. 14. Panter, R, A, and R. H. Symons. 1966. Isolation and Properties of a DNA—containing rod—shaped bacteriophage. Aust. J. Biol. Sci. 19: 565—573. 15. Prait, D. 1969. Genetic of single—stranded DNA bacteriophage. Annu. Rev. Genet. 3: 345—362. 16. Prait, D and W S- Erdahl. 1968. Genetic control of baeteriophage M13 DNA synthesis. Md. Blol. 37:181—200. 17. Ray, D. S., H, P. Bscheider, and P.. H. Hofschneider. 1966. Replication of the single—stranded DNA of the male—specific bacteriophage M13. Isolation of intracellalar forms of phage—specific DNA. J. Mol. Biol. 21: 473—483. 18. Stone, A. B 1967. Some factors which influence the replication form of bacteriophage ?x174. Biochem.Biophys.. Rea.. Commun. 26: 247—254. 19. Tzagoloff, H., and D. Pratt. 1964. The initial steps’ in infection with coliphage M13. Viology 24: 372—380. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75790 | - |
dc.description.abstract | 中文摘要 水稻白葉枯病是水稻的重要病害之-。目前為止,尚無適當的防治方法。本研究針對此目的,利用病原菌的噬菌體做為水稻白葉枯病的生物防治。 本研究首先探測病原菌受X f 感染後,其病原性降低的程度,發現 80%被感染的接種源,其病原性降低為原有的1%。在進一步探討其原因時,發現已被 X f 感染之細菌菌落較一般未感染的菌落為小,且其菌落形成能力減少為原有的30-40% .此點可解釋一部分病原性降低的原因,也是對生物防治不利的條件。 為進一步研究病原性降低的其他原因,本研究並比較了有 X f 感染及無X f 感染的細菌培養液,其DNA、RNA 及蛋白質生合成的能力。 DNA 生合成能力於感染前 20 分鐘呈直線增加,以後速度則稍微降低。RNA生合成能力於感染的1小時之內普遍稍降 5-10%。蛋白質生合成能力則降低為原來的 70-50%。故 推測蛋白質可能與細菌的病原性有關。 此外,另一與生物防治有密切關係的問題是:感染菌能否分裂?分裂後能否將噬菌體傳到後代?本研究證實,感染菌以一較正常菌為慢的速度不斷分裂,但分裂使僅其中-個子細胞獲有釋放感染體( infectious particles )的能力。此性質亦不利於生物防治上的利用。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:25Z (GMT). No. of bitstreams: 0 Previous issue date: 1974 | en |
dc.description.tableofcontents | 目錄 中文摘要- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -工 英文摘要- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - II 一、緒言- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - l 二、實驗材料與方法- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 (一)細菌及噬菌體- - - - - - -- - - - - - - - - - - - - - - - - - - - - 3 (二)實驗試藥- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 (三)培養基的備置- - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 (四)細菌及噬菌體的培養- - - - - - - - - - - - - - - - - - - - - - - 4 (五)細菌及噬菌體濃度的測定- - - - - - - - - - 4 (六)細茵病原性的測定- - - - - - - - - - - - - - - - - - - 5 (七)辨別被 X f 感染或無感染細菌細胞的方法- - - - - - - - - - - - - - -5 (八)被X f 感染細胞數目的測定(infective center )- - - - - - - - - - - - - 6 (九)DNA生合成速率的測定- - - - - - - - - - - - - - - - - - - - 6 (十)RNA生合成速率的測定- - - - - - - - - - - - - - - - - - 7 (十一) 蛋白質生合成速率的測定- - - - -- - - - 8 (十二)同位素測定法 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 8 三、實驗結果- - - - - - - - - - - - - -- - - -- - - - - - - - - 9 (一)X f 感染後對細菌病原性的影嚮- - - - - -- - - -- - - - - - - 9 (二)X f 感染後對細菌菌落及細菌生長分裂的影嚮 - - -- - - -- - - - - - 11 (三)X f 感染後對細菌重要新陳代謝的影嚮 - - - - - 13 1. DNA生合成的能力- - - - - - - - - - - - - - - - - - - - - - - 14 2. RNA 生合成的能力- - - - -- - - - - - - - - - - - - - - - -14 3. 蛋白質生合成的能力- - - - - - - - - - - - - - - - - - - - - - 15 (四) 被X f 感染之細菌細胞能否分裂,分裂時能否將噬菌體傳到後代- - - - - - 19 四、討論- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -23 五、參考文獻- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 27 | |
dc.language.iso | zh-TW | |
dc.title | 噬菌體 X f 的感染對水稻白葉枯病病原細菌病原性的影嚮 | zh_TW |
dc.title | Effect of Xf Infection on The Pathogenicity of Xanthomonas oryzae | en |
dc.date.schoolyear | 62-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 70 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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