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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳武聖 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:21Z | - |
| dc.date.available | 2021-07-01T08:15:21Z | - |
| dc.date.issued | 1991 | |
| dc.identifier.citation | 黃玉芬，1988．水稻內的酸性磷酸?．國立臺灣大學植物科學研究所碩士論文。 Baker, J. E. and T. Takeo, 1973. Acid phosphatase in plant tissues I. Changes in activity and multiple forms in tea leaves and tomato fruit during maturation and senescence. Plant and Cell Physio. 14: 459-471. Barlow, S. B. and R. E. Triemer, 1986. Phosphatase localization in the endomembrane system of the dinoflagellate Crypthecpdinium cohnii. J. Histochem. Cytochem. 34: 1021-1027. Barrett-Lennard, E. Q. and H. Greenway, 1982. Partial separation and characterization of soluble phosphatases from leaves of wheat grown under phosphorus deficiency and water deficit. J. Exp. Bot. 33: 694-704. Bentwood, B. J. and J. Cronshaw, 1976. Biochemistry and cytocheuiical localization of acid phosphatase in the phloem of Nicotiana tabacum. Planta 130: 97-104. Berjak, P., 1972. Lysosomal compartmentation : ultrastructural aspects of the origin, development, and function of vacuoles in root cells of Lepidium sativum. Ann. Bot. 36: 73-81. 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Control of ribo-nuclease and acid phosphatase by auxin and abscisic acid during senescence of rhoeo leaf sections. Plant Physiol. 46: 806-811. Deltour, R., S. Fransolet and R. Loppes, 1981. Inorganic phosphate accumulation and phosphatase activity in the nucleus of maize embryo root cells. J. Cell Sci. 47:77-90 Figier, J., 1972. Electron microscopic localization of acid phosphatase in the petiolary glands of Impatiens holstii. Planta 108: 215-226. Flasinski, S. and J. Rogozjnska, 1988. Acid phosphatase activity in oilseed rape depending on phosphorus content in the medium and the plant. Acta. Physiol. Plant 10:3-10. Forti, G., 1961. Partial purification of a pyrophosphatase from spinash leaves. Biochim.biophys.Acta 48:200-202. Gabard, K. A. and R. L. Jones, 1986. Localization of phytase and acid phosphatase isoenzymes in aleurone layers of barley. Physiol. Plant. 67: 182-192. Gomori, G., 1939. Microtechnical demonstration of phosphatase in tissue sections. Proc. Soc. Exp. Biol. 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A comparative study of multiple acid phosphatases in cellular fractions of bean phaseolus-vulgaris hypocotyl. Ann. Bot. 45: 369-382. Newmark, M.Z. and B.S. Wenger, 1960. Preparation and some properties of an acid phosphatase from white lupine seedlings.Arch.Bjochen.Biophys.89:110-117. Ozutsumi, A., T. Sato, N. Ogura and H. Nakagawa, 1983. Immunological properties of two acid phoaphatases from tomato fruit. Agric. Biol. Chem. 47: 1137-1138. Ohtsuka, T. and H. Saka, 1988. Undestructive measurement of acid phosphatase activity in the root of rice seedling. Jpn. J. Corp. Sci .57:722-727. Palma, H. S., S. A. Rodrigues and A. Rossi, 1982. Kinetic properties of acid phosphatase from scutella of germinating maize seeds. Phytochemistry. 21: 1245-1247. Pan, S. M., 1985. Phosphatases in spinach leaves sub-cellular localization and the stress effect. Bot Bull Acad Sin 26: 185-194. Pan, S. H., Y. F. Huang and Y. R. Chen, 1987. 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Properties of acid phosphatase from scutella of germinating maize seeds. Phytochemistry. 20: 1823-1826. Saeed, A., Z. A. Khan, I. U. Khan and M. R. Baig, 987 Purification and characterization of acid phosphatase from sorghum (Sorghum vulgare) seed. Pak. J. Sci. Ind Res. 30: 411-416. Sexton, R., J. Cronshaw and J. L. Hall, 1971. A study of the biochemistry and cytochemical localization of β-glycerophosphatase activity in root tips of maize and pea. Protolasma 73: 417-441. Shaw, J. G., 1966. Acid phosphatase from tobacco leaves. Arch. Biochem. Biophys. 117: 1-9. Sheldon, H., H. Zetterqvjst and D. Brandes, 1955. Histochemical reactions for electron microscopy: acid phosphatase. Exp. Cell Res. 9: 592. Spurr, A. R., 1969. A low viscosity epoxy resin embedding medium for elecrron microscopy. J. Ultrast. Res. 26: 31-34. Taussky, H. H. and E. Shorr, 1953. A microcolorimetric method for determination of inorganic phosphorus. J. Biol. Chem. 202: 675-685 Tandler, C. J. and A. J. Solari, 1969. Nucleolar orthophospate ions electron microscope and diffraction studies. J. Cell Biol. 41: 91-108. Teno, A. M., M. S. Palma and A. Rossi, 1987. Acid hoshatase from maize scutellum: properties as a function of seed germination. Phytochemistry. 26:55-58. Ueki, K. and S. Sato, 1977. Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells. Plant Cell Physiol. 18: 1253-1263. Ullah, A. H. and D. M. Gibson, 1988. Purification and characterization of acid phosphatase from cotyledons of germinating soybean seeds. Arch. Biochem. Biophys. 260: 514-520. Vieira da Silva, J., A. V. Naylor and P. J. Kramer, 1974. Some ultrastructural and enzymatic effects of water stress in cotton (Gossvpium hirsutum L.) leaves. Proc. Nat. Acad. Sci. 71: 3243-3247. Vashitani, I. and S. Sato, 1976. Reliability of the lead salt precipitation method of acid phosphatase localization in plant cells. Protoplasma 89: 157-170. Valter, H., 1969. Ultrastruetural localization of acid hoshatase in cultured cells of Daucus carota. Planta. 88:91-102. Vebern, L. and M.Osborn, 1969.The reliability of molecular weight determination by dodecl sulfate-polyacrylamide gel electrophoresis. J. Biol .Chem. 244:4406-4412. Ymagata, H., T. Kunisuke and K. Zenzaburo, 1979. Isoenzymes of acid phosphatase in aleurone particles of rice grains and their interconversion. Agric. Biol. Chem. 43: 2059-2066. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75783 | - |
| dc.description.abstract | 水稻酸性磷酸?屬多型性，各種同功?等電站皆落在4.5-6.5之間。利用硫酸銨沉澱、膠體過濾層析、集焦管柱層析及聚丙烯醴胺電泳分離純化，並以膠體過瀘層析估計酵素活性分子量在130kd附近。SDS-PAGE分析得有70kd及62kd次體，推測屬異元雙體結構。 部份純化的酸性磷酸?進行特性探討，其活性最適酸鹼度在5.0左右。不同受質的測試以para-nitrophenyl phosphate反應最佳，km值為0.69mM。在60℃下加熱5分鐘，酵素活性降低50％左右。HnSO4促進酵素活性約50％，而HgCl2則抑制90％的活性。用細胞化學定位檢出根冠區中酵素主要聚集在細胞壁及細胞核。外層遊離的細胞則各種胞器都有酸性磷酸?存在。根部分生組織內酵素主要分佈於細胞壁。延長區內以液胞、細胞膜以及細胞核較顯著。至於葉片組織中酸性磷酸?在表皮、葉肉及即將瓦解的細胞均有出現。有關酸性磷酸?的次細胞分佈及其生理意義則在文中進一步討論。 | zh_TW |
| dc.description.abstract | Acid phosphatase (EC 3.1.3.2) extracted from rice seedlings consisted of more than ten kinds of isozymes in zymograni of polyacrylamide gel electrophoresis.They were stepwisely purified by using ammonium sulfate precipitation. Ultro gel filtration, DEAE-trisacryl column and chromatofucsing gel chromatographies. One of the isozymes with optimal pH at 5.0, possessed high affinity to p-nitrophenyl phosphate. FPLC separation showed that its molecular weight was around l30kd, and SDS-PAGE showed that it contained equivalent peptide subunits of apparent Mr 70 and 62 kd. The purified acid phosphatase is heat labile, and its Km is 0.69mM at pH 5.0. Mn•enhances the activity of acid phosphatase, and Hg•is the strongest inhibitor and NaF has only partial inhibitorily effect. Localization of acid phosphatases b the method of Goniori showed that they mainly distributed in cell wall, nucleus and vacuole in root cap. In ground meristem, acid phosphatases were present in cell wall, and in the region of elongation, they were found in tonoplast, cell membrane and nucleus. While in leaf, acid phosphatases appared in vacuole, oil drop, starch grain and cytoplasm. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:21Z (GMT). No. of bitstreams: 0 Previous issue date: 1991 | en |
| dc.description.tableofcontents | 壹、中文摘要……………………………………………………………………………………………? 貳、英文摘要……………………………………………………………………………………………? 參、前言…………………………………………………………………………………………………1 肆、材料與方法…………………………………………………………………………………………6 伍、結果…………………………………………………………………………………………………15 陸、討論…………………………………………………………………………………………………45 柒、參考文獻……………………………………………………………………………………………52 | |
| dc.language.iso | zh-TW | |
| dc.title | 水稻酸性磷酸?的純化與定位 | zh_TW |
| dc.title | Purification and Localization of Acid Phosphatase in Rice | en |
| dc.date.schoolyear | 79-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 66 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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