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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75777完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 張淑慧 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:19Z | - |
| dc.date.available | 2021-07-01T08:15:19Z | - |
| dc.date.issued | 1989 | |
| dc.identifier.citation | Reference Aloj, M. S., Edelhoch, H., and Ingham, K. C. (1973). The rate of dissociation and reassociation of the subunits of human chorionic gonadotropin. Arch. Biochem. Biophys. 159, 497-504. Ascoli, M., Liu, W. K., and Ward, D. N. (1977). Isolation and recombination of ovine lutropin subunits with complete recovery of biological activity. J. Biol. Chem. 252, 5280-5286. Breton, B. (1981). A study of salmon (Oncorhynchus tshawytscha) pituitary gonadotropin dissociation. Gen. Comp. Endocrinol. 45, 147-152. Burke, W. H., Papkoff, H., Licht, P., and Bona-Gallc, A. (1979). Preparation and properties of luteinizing hormone (LH) subunits from the turkey (Meleagris gallopavo) and their recombination with subunits of ovine LH. Gen. Comp. Endocrinol. 37, 501-507. Burzawa-Gerard, E. and Fontaine, Y. A. (1972). The gonadotropin of lower vertebrates. Gen. Comp. Endocrinol., Suppl. 3, 715-728. Burzawa-Gerard, E., Goncharov, B., Dumas, A., and Foutain, Y.A. (1976). Further biochemical studies on carp gonadotropin (c-GTH): Biochemical and biological comparison of c-GTH and a gonadotropin from Asipenser stellatus Pall Chondrostei). Gen. Comp. Endocrinol. 29, 498-505. Burzawa-Gerard, E., and Fontaine, Y. A. (1976). Formation d’une molecule hybride douee d’une activite gonadotrope sur la grenouille, a partir de la sous-unite a de l’hormone luteinisante bovine et d’une sous-unite de l’hormone gonadotrope d’un poisson teleosteen. C. R. Acad. Sci. Paris Ser. D 282, 97-100. Chang, Y. S., and Huang, F. L. (1982). The mode of action of carp gonadotropin on the stimulation of androgen production by carp testis in yitro. Gen. Comp. Endocrinol. 48, 147-153. Chang, Y. S., Huang, C. J., Huang, F. L., and Lo, T. B. (1988) Primary strutures of carp gonadotropin Subunits deduced from cDNA nuleotide sequence. Int. J. Peptide. Protein Res. 32, 556-564. Chang, Y. S., Huang, C. J., Liu, C. S., Huang, F. L. and Lo, T. B. (1989). Purification, characterization and molecular cloning of gonadotropin subunits of silver carp (Hypophthalmichthys molitrix). Gen. Comp. Endocrinol. (in press). Erlanger, B. F., Borek, F., Beiser, S. M., and Lieberman, S. (1959). Steroid-protein conjugates. II. Preparation and characterization of conjugates of bovine serum album with progesterone, deoxy-corticosterone and esterone. J. Biol. Chem. 234, 1090-1094. Haider, S. G., and Blum, V. (1977). On the evolution of the pituitary gonadotropin system in lower vertebrates. Invest. Pesq. 41, 185-204. Huang, F. L.., and Chang, Y. S. (1980). The gonadotropic stimulation of androgen production on carp testis in vitro. Proc. Natl. Sci. ROC. 4, 392-400. Huang, F. L., Huang, C. J., Lin, S. H., Lo, T. B., and Papkoff, H. (1981). Isolation and characterization of gonadotropin isohormones from the pituitary gland of pike eels. Int. J. peptide protein Res. 18, 69-78. Huang, F. L., Chang, Y. S., Chang, G. D., and Lo, T. B. (1982) Properties of subunits of pike eel gonadotropin. Proc. Natl. Sci. Counc. ROC. 6, 30-36. Idler, D. R., and Ng, T. B. (1983). Teleost gonadotropins: isolation biochemistry, and function. Fish Physiology. (Hoar, W. S., and Randall, D. J.), 187-221. Jolles, J., Burzawa-Gerard, E., Fontaine, Y. A., and Jolles, P. (1977). The evolution of gonadotropins: some molecular deta concerning a concerning a non-mammalian pituitary gonadotropin, the hormone from a teleost fish (Cyprinus carpio L.). Biochimie. 59, 893-898. Liao, T. H., and Pierce, J. G. (1970). The presence of a common type of a subunit in bovine thyoid-stimulating and luteinizing hormones. J. Biol. Chem. 245, 3275-3281. Licht, P., Farmer, S. W., and Papkoff, H. (1978). Biological activity of hybrid combinations of ovine and sea turtle LH subunits. Gen. Comp. Endocrinol. 35, 289-294. Licht, P., Papkoff, H., Farmer, S. W., Muller, C. H., Tsui, H. W., and Crews, D. (1979). Evolution of gonadotropin structure and function. Recent. Prog. Horm. Res. 33, 169-248. Liu, C. S., Chang, C. H., Chen, J. M., Chen, S. W., Chang, Y. S., Huang, F. L., and Lo, T. B. (1989). Pike eel (Muraenesox cinereus) gonadotropin: Amino acid sequences of both a and B subnuits. Eur. J. Biochem. (in submission). Lowry, O. H., Rosebroug, N. T., Farrand, A. L., and Randall, R. J. (1951). Protein mearsurement with phenol reagent. J. Biol. Chem. 193, 265-275. Marchelidon, J., Salesse, R., Garnier, J., Burzawa- Gerard, E., and Fontaine Y. A. (1979). Zoological origin of gonadotropin subunits and association kinetics. Nature. (London). 281, 314-315. Morgan, F. J., and Canfield, R. E. (1971). Nature of the subunits of human chorionic gonadotropin. Endocrinology. 88, 1045-1053. Moyle, W. R., and Ramachandran, J. (1973). Effect of LH on steroidogenesis and cyclic AMP accumalation in rat Leydig cell preparation and mouse Leydig cells. Endocrinology. 93,127-134. Papkoff, H. (1974). Chemical and biological properties of subunits of pregnant mare serum gonadotropin. Biochem. Biophys. Res. Commun. 58, 397-404. Papkoff, H., Farmer, S. W., and Licht, P. (1976). Isolation and characterization of follicle-stimulating hormone and luteinizing hormone and its subunits from snapping turtle (Chelydra serpentina) pituitary. Endocrinology. 98, 767-777. Pierce, J. G., and Parsons, T. F. (1981). Glycoprotein hormones: struture and function. Ann. Rev. Biochem. 50, 465-495. Ryan, R. J., Keutmann, H. T., Charlesworth, M. C., McCormick, D. J., Milius, R. P., Calvo, F. O., and Vutyavanih, T. (1987). Structure-function relationships of gonadotropins. Recent. Prog. Horm. Res. 43, 383-429. Ryan, R. J., Charlesworth, M. C., McCorimick, D. J., Milius, R. P., and Kentmann, H. T. (1988). The glycoprotein hormones: recent studies of structure-function relationships. FASEB. 2, 2661-2669. Suzuki, K., Kawauchi, H., and Nagahama, Y. (1988a). Isolation and characterization of two distinct gonadotropins from chum salmon pituitary glands. Gen. Comp. Endocrinol. 71, 292-301. Suzuki, K., Kawauchi, H., and Nagahama, Y. (1988b). Isolation and characterization of subunits from two distinct salmon gonadotropins. Gen. Comp. Endocrinol. 71, 302-306. Swaminathan, N., and Bahl, O. P. (1970). Dissociation and recombination of the subunits of human chorionic gonadotropin. Biochem. Biophys. Res. Commun. 40, 422-427. Trinh, K. Y., Wang, N. C., Hew, C. L., and Crim, L. W. (1986) Molecular cloning and sequencing of salmon gonadotropin subunit. Eur. J. Biochem. 159, 619-624. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75777 | - |
| dc.description.abstract | 摘要 本實驗的目的是將幾種動物之腦下腺性腺刺激素（Gonadotropin, GTH）的次單元己分別加以重組，然後測定這些重組分子促進睪九產生雄性素的能力，以期瞭解各次單元的特性及其在生物活性上所扮演的角色。 鯉科的鯉魚、白鰱和大頭鰱GTH之次單元可以任意置換，而不影響促進離體鯉魚睪丸組織產生雄性素的能力，顯示鯉科中此三種魚類GTH之次單元的屬性非常的類似。 在海鰻與大頭鰱GTH次單元的重組分子中，海鰻GTHα次單元與大頭鰱GTHβ次單元之重組分子及大頭鰱GTHα次單元與海鰻GTHβ次單元之重組分子均具有促進離體鯉魚睪丸組識產生雄性素之能力，但前者之活性較後者為佳。 鯉魚GTH與羊黃體成長激素（Luteinizing hormone, LH）次單元的重組實驗中發現，除了鯉魚GTH的α，β次單元之重組分子，對離體鯉魚睪丸生生成雄性素具有顯著之促進作用外，羊LHα次單元與鯉魚GTHβ次單元的重組分子以及鯉魚GTHα次單元與羊LHβ次單元重組分子也具有促進離體鯉魚睪丸組識生成雄性素之能力，但其活性較弱，分別為鯉魚GTHα及β次單元之結合分子活性的60.3％及29.5％，至於羊LHα次單元與β次單元之結合分子則全然無活性。而在老鼠萊氏細胞生成雄性素之測定系統中，若以羊LH的α次單元與β次單元的結合分子的活性為100%。則鯉魚GTHα次單元與羊LHβ次單元結合分子的活性為10.0％，而羊LHα次單元與鯉魚GTHβ次單元的結合分子則幾無活性。 綜合本實驗利用不同種動物的GTHα次單元及β次單元進行重組之結果顯示，α次單元則在不同種類間仍具有相似性，故可彼此互換而形成具有生物活性的雜種GTH分子，而β次單元，則有明顯的種別性差異，故可決定激素的屬性。此等事實則顯示，脊椎動物的GTH在演化過程中，α次單元較為保守，但β次單元則顯得分歧。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:19Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
| dc.description.tableofcontents | 目錄 摘要. . . . . . . . . . . . . . . . . . 1 緒言. . . . . . . . . . . . . . . . . . 3 材料與方法. . . . . . . . . . . . . . . . . . 8 一、藥品. . . . . . . . . . . . . . . . . . 8 二、GTH及次單元的製備. . . . . . . . . . . . . . . . . . 8 三、GTH次單元的重組. . . . . . . . . . . . . . . . . . 9 四、離體鯉魚睪丸組識雄性素生合成測定系統. . . .. . 10 五、大白鼠萊氏細胞睪固酮生合成測定系統. . . . .. . . 10 六、睪固酮抗體的製備. . . . . . . . . . . . . . . . . . 11 七、睪固酮之放射免疫測定. . . . . . . . . . . . . . . . 11 八、有效劑量..(ED..）之計算. . . . . . . . . . . . . . . . 13 九、蛋白質含量測定. . . . . . . . . . . . . . . . 13 結果. . . . . . . . . . . . . . . . 14 一、鯉魚．大頭鰱及白鰱GTH次單元之重組結合體促進離體鯉魚睪丸組織產生雄性素之能力. . . . . . . . . . . . . 14 二、大頭鰱與海鰻GTH次單元之重組結合體促進離體睪丸組織產生雄性素之能力. . . . . . . . . . . . . . . . 14 三、羊LH及鯉魚GTH次單元重組結合體促進睪丸組識產生雄性素之能力. . . . . . . . . . . . . . . . 19 討論. . . . . . . . . . . . . . . . 23 引用文獻. . . . . . . . . . . . . . . . 26 | |
| dc.language.iso | zh-TW | |
| dc.title | 異種間性腺刺激素之次單元之重組分子促進睪丸?生雄性素之效力研究 | zh_TW |
| dc.title | Stimulatory Effect on Androgen Production in Testis by Hybrid Gonadotropins Reconstituted from Different Species | en |
| dc.date.schoolyear | 78-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 38 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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