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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 薑鈴 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:16Z | - |
| dc.date.available | 2021-07-01T08:15:16Z | - |
| dc.date.issued | 1990 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75772 | - |
| dc.description.abstract | 九孔（Haliotis diversicolor）是台灣重要的經濟性貝類，除了西部沙岸外，沿海的岩礁海岸都有天然九孔的分佈。本實驗主要以限制內切核酸?（restriction endonucleases）處理九孔mtDNA ，分析本省九孔的族群關係。本實驗的九孔來自四個具有地理隔絕性區域（臺北縣鼻頭角，宜蘭縣頭城，花運縣磯崎，台東縣成功）的野生九孔及來自宜蘭的養殖種九孔。 從成熟母貝卵巢中抽取 mtDNA ，用五種限制內切?（BamHI, HindⅢ， KPnI , SstI , XbaI ）處理60隻九孔mtDNA 初步得到九孔mtDNA分子量大小為17.33一19.57Kb ．再由切片段經數學方法轉換成核?酸分歧度（P值， nucleotide diversity ）。發現各地區間的九孔有演化發生不連續（ phylogenetic discontinuity ）的現象，顯示九孔有因地理隔絕而造成族群間的分化（differentiation）的結果。核?酸分歧度經UPGMA一Cluster 整合後繪成演化發生系統樹（evolutionary phylogenetic trees ) ，得到宜蘭頭城地區的野生九孔族群內的 mtDHA?切型式組成（composite digestion patterns）最保守（conserved）和穩定，表示此區域的地理環境隔絕性較強。花蓮、台東地區的野生九孔有基因流動（ gene flow）的現象。而養殖種的九孔來源可能是取自宜蘭頭城的野生種。 再用十種限制內切（ BamH I , Bc1 I , Bgl II，BstE II, EcoR I , Hind III， Kpn I, Pvu II， Sst I , Xba I）處理九孔mtDNA ，得到一個有26個切片段的限制內切?切圖譜（restriction endonuclease map ）。並配合族群分析的?切片段結果，得到九孔mtDNA在核?酸序列（nucleotide sequences）上至少有五種以上的變異情形。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:16Z (GMT). No. of bitstreams: 0 Previous issue date: 1990 | en |
| dc.description.tableofcontents | 摘要........................ 1 第一章 緒論........................ 2 第二章 mtDNA抽取與材枓來源 ........................ 15 第三章 台灣九孔族群的分析 ........................ 23 第四章 九孔mtDNA 限制內切?圖譜的建立與探討........................ 69 第五章 討論........................ 96 謝辭........................ 101 參考文獻........................ 102 附錄（一）........................ 107 附錄（二）........................ 109 | |
| dc.language.iso | zh-TW | |
| dc.title | 應用粒線體DNA分析臺灣的九孔族群關係 | zh_TW |
| dc.date.schoolyear | 78-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 102 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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