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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | 洪千惠 | zh_TW |
dc.date.accessioned | 2021-07-01T08:15:11Z | - |
dc.date.available | 2021-07-01T08:15:11Z | - |
dc.date.issued | 1990 | |
dc.identifier.citation | Balbas, P., X. Soberon, E. Merino, M. Zurita, H. Lomeli, F. Valle, N. Flores, and F. Bolivar. 1986. Plasmid vector pBR322 and its special-purpose derivatives -- a reveiw. Gene 50:3-40.
Barany, F. 1985. Single-stranded hexameric linkers: a system for in-phase insertion mutagenesis and protein engineering. Gene 37:111-123. Baum, E.Z., S. F. Love, and D. M. Rothstein 1988. Temporally regulated tandem promoters in Micromonospora echinospora. J. Bacteriol. 170:71-77. Berg, D. E., R. Jorgensen, and J. Davies (1978). In Microbiology-1978 (Schlessinger, D., ed.), pp. 13-15. American Society for Microbiology, Washington, D.C. Birnboim, H.C. and J. Dolly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res. 7:1531. Bolivar, F., R. L. Rodriguez, P. J. Greene, M. C. Betlach, H. L. Heymeker, H. W. Boyer. 1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95-113. Boyer, H. W. and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459. de Boer, H. A., L. J. Comstock, and M. Vasser. (1983). The tac promoter: a functional hybrid derived from the trp and lac promotors. Proc. Natl. Acad. Sci. USA 80:21-25. Dong, X., D. D. Womble, and R. H. Rownd. 1987. Transcriptional pausing in a region important for plasmid NR1 replication control. J. Bacteriol. 169:5353-5363. Goeddel, D. V., H. M. Shepard, E. Yelverton, D. Leung, and R. Crea (1980) Nucleic Acids Res. 8:4057-4074. Gottesman, M., A. Oppenheim, and D. Court. 1982. Retroregulation: Control of gene expression from sites distal to the gene. Cell 29:727-728. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557. Hanahan, D. 1985. Techniques for transformation of E.coli. In DNA cloning: A practical approach (ed. D.M. Glover), Vol. 1, P. 109. IRL Press, Oxford. Hattori, M. and Y. Sakaki. 1986. Dideoxy sequencing method using denatured plasmid templates. Analytical Biochem. 152:232-238. Henikoff, S. 1978. Methods Enzymol. 155:156 Henikoff, S. 1984. Gene 28:357 Ho, Y. S. and M. Rosenberg. 1985. Characterization of a third, cll-dependent, coordinately activated promoter on phage involved in lysogenic development. J. Biol. Chem. 260:11838-11844. Ho, Y. S., D. Wulff, and M. Rosenberg. 1983. Bacteriophage protein cll binds promoters on the opposite face of the DNA helix from RNA polymerase. Nature 304:703-708. Hopwood, D. A., M. J. Bibb, K. F. Chater, G. R. Janssen, F. Malpartida, and C. P. Smith. 1986. Regulation of gene expression in antibiotic-producing Streptomyces, P. 251-276. In I. R. Booth and C. F. Higgins (ed.), Regulation of gene expression: 25 Years on. Cambridge University Press, Cambridge. Ish-Houowitz, D. and J. F. Burke. 1981.Rapid and efficient cosmid vector cloning. Nucleic Acid Res. 9:2989 Janssen, G. R., M.J. Bibb, C. P. Smith, J. M. Ward, T. Kieser, and M. J. Bibb. 1985. Isolation and analysis of Streptomyces promoters, p. 392-396. In L. Lieve, P. F. Bonaventre, J. A. Morello, S. Schlesinger, S. D. Silver, And C. Wu (ed.), Microbiology--1985. American Society for Microbiology, Washington, D.C. Kado, C. I. and S. T. Liu. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Becteriol. 145:1365-1374. Kennell, D. 1986. The instability of messenger RNA in bacteria: in: Maximizing Gene Expression (W. Reznikoff and L. Gold, eds.), pp 101-143, Butterworths, Boston Lodge, J. K., T. Kazic, and D. Berg. 1989. Formation of supercoiling domains in plasmid pBR322. J. Bacteriol. 171:2181-2187. Mandel, M. and A. Higa. 1970. CaCl2 dependent Bacteriophage DNA infection. J. Mol. Biol. 53:154 Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. Miller, I. H. and W. S. Reznikoff. 1980. The Operon. Cold Spring Harbor Laboratory. Cold Spring Harbor, N. Y. Muller, D., B. Hofer, A. Koch, and H. Koster. 1983. Aspect of the Mechanism of acid-phenol extraction of nucleic acids. Biochimica et Biophysica Acta. 740:1-7. Oka, A., H. Sugisaki, and M. Takanami. 1981. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147:217-226. Ponnambalam, S. and S. Busby. 1987. RNA polymerase molecules initiating transcription at tandem promoters can collide and cause premature transcription termination. FEBS lett. 212:21-27. Pribnow, D. (1975). Bacteriophage T7 early promoters: Nucleotide sequences of two RNA polymerase binding sites. J. Mol. Biol. 99:419-443. Rosenberg, M. and D. Court. 1979. Regulatory sequences involved in the promotion and termination of RNA transcription. Ann Rev. Genet. 13:319-353. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Moleculor Cloning. A Laboratory Munual. Second edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-teminating inhibitors. Proc. Natl. Acad. Sci. USA. 74: 5463-5467. Sawers, G. and Rock, A. (1989). Novel transcriptional control of the pyruvate formate -lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression. J. Bacteriol. 171:2485-2498. Schakker, H., C. Gray, and Herrmann, K. (1975). Nucleotide sequence of an RNA polymerase binding site from the DNA of becteriophage fd. Proc. Natl. Acad. Sci. USA 72:737-741. Shine, J. and Dalgarno, L. (1975). Determinants of cistron specificity in bacterial ribosomes. Nature 254:34-38. Simons, G., E. Remaut, B. Allet, R. Devos, and W. Fiers (1984). High level expression of Human interferon-γ in E. coli under control of the PL promoter of bacteriophage λ. Gene 28:55-64. Stuber, S. and H. Bujard. 1980. Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc. Natl. Acad. Sci. USA 78:167-171. Vieira, J. and J. Messing. 1982. The pUC plasmids, and M13mp7-derived system for insertion mutangenesis and sequencing with synthetic universal primers. Gene 19:259-268. Winnacker, E.-L. 1987. From Genes to Clones, Introductin to Gene Technology. VCH, Verlagsgesellschaft. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75760 | - |
dc.description.abstract | 質體pUC4-KISS所攜帶的Aminoglycoside 3'-phosphotransferase(APHI)基因在大腸桿菌中的表現量可高達全部蛋白質的百分之四十.然而,發現在不同的方向,不同的載體上,APHI基因的表現量顯著的降低,故推測質體pUC4-KISS中基因上游的某些核酸序列會影響APHI基因的表現.因此在本實驗中利用限制?切割及Exolll-SI方法刪除DNA片段;確實基因上游的核酸序列會影響APHI的表現.此外核酸定序分析發現lacP是位在APHI基因的上游,此發現與前人發表的圖譜不同.因此APHI基因之高表現可能是由於lacP及APHI基因產生Operon fusion之結果.除了lacP外,在更上游的核酸序列中,有兩個區域似乎也能影響APHI的表現,刪除其中41 bp的DNA使得APHI基因表現降低百分之八十.但是如果此41 bp之序列連同上游650 bp之序列一同刪除後,APHI之表現則維持在40%.顯示在41 bp上游序列,可能對APHI基因之表現有抑制作用,本研究主要是證實,基因的表現除了與本身的起動子有關外,基因上游的起動子和核酸序列也扮演著重要的角色. | zh_TW |
dc.description.abstract | The expression level of the gene encoding aminoglycoside 3'-phosphotransferase (APHI) in pUC4-KISS reached approximately 40% of total protein in Escherichia coli. The expression level decreased, however, when the orientation of the gene in pUC4-KISS was reverted or when the gene cartridge was cloned into the Pstl site of pUC19. Sequencing analysis revealed that the lac promoter was situated upstream from the APHI gene. This finding indicated that the map of pUC-4-KISS reported previously was incorrect. The high expression of APHI was probably due to an operon fusion between lacZ and APHI gene. The P5 promoter of pBR322 which was located upstream from the lac promoter also affected the expression of APHI. Deletions in this promoter region lowered the gene expression by 25%. However, the DNA upstream from the P5 promter seemed to inhibit the expression. Restriction deletion studies revealed that deletions in this region actually increased the expression of APHI gene in pUC4-KISS. This study demonstrated that the upstream sequence of the APHI was essential for the high expression of the gene. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:11Z (GMT). No. of bitstreams: 0 Previous issue date: 1990 | en |
dc.description.tableofcontents | 中文摘要……………………………………1 英文摘要……………………………………2 材料與方法……………………………………11 結果……………………………………27 討論……………………………………51 參考資料……………………………………59 | |
dc.language.iso | zh-TW | |
dc.title | Aminoglycoside 3'-phosphotransferase基因在質體pUC4-KISS中表現之探討 | zh_TW |
dc.title | Expression of Aminoglycoside 3'-phosphotransferase Gene of pUC4-KISS in E. coli | en |
dc.date.schoolyear | 78-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 68 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
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