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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | 曾冬筍 | zh_TW |
dc.date.accessioned | 2021-07-01T08:15:06Z | - |
dc.date.available | 2021-07-01T08:15:06Z | - |
dc.date.issued | 1990 | |
dc.identifier.citation | 1. Amin J. J Ananthan, R Voellmy 1988. Key features of heat shock regulatory elements. Mol Cell Biol 8:3761-3769. 2. Ausubel FM, R Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, K Struhl (eds): 1987. Current protocals in molecular biology. John Wiley & Sons, NY. 3. Baszczynski CL, DB Walden 1982. Regulation of gene expression in corn (Zea mays L.) by heat shock. Can J Biochem 60:569-579. 4. Campbell W, G Gowri 1990. Codon usage in higher plants, green algae, and Cyanobacteria. Plant Physiol 92:1-11. 5. Chou M, YM Chen, CY Lin 1989. Thermotolerance of isolated mitochondria associated with heat shock proteins. Plant Physiol 89:617-621. 6. Cooper P, THD Ho, RM Hauptmann 1984. Tissue specificity of the heat-shock response in maize. Plant Physiol 75:431-441. 7. Corce V, A Pellicer, RA Vel, M Meseleson 1981. Integration, transcription, and control of a Drosophilla heat shock gene in mouse cells. Proc Natl Acad Sci USA 78: 7038-7042. 8. Czarnecka E, L Edelman, F Sch?ffl, JL Key 1984. Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs. Plant Mol Biol 3:45-58. 9. Czarnecka E, RT Nagao, JL Key, WB Gurley 1988. Characterization of Gmhsp26-A, a stress gene encoding a divergent heat shock protein of soybean: Heavy-metal-induced inhibition of intron processing. Mol Cell Biol 8:1113-1122. 10. Czarnecka E, WB Gurley, RT Nagao, LA Mosquera, JL Key 1985. DNA sequence and transcript mapping of a soybean gene encoding a small heat shock protein. Proc Natl Acad Sci USA 82:3726-3730. 11. Dulder R, AA Travers 1984. Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies. Cell 38:391-398. 12. Ernst JF 1988. Codon usage and gene expression. TibTech 6:196-199. 13. Feinberg AP, B Nogelstein 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6-13. 14. Feinberg AP, B Nogelstein 1984. ADDENDUM A technique for radiolabeling DNA restriction endonuclease fragmentsto high specific activity. Anal Biochem 137:266-267. 15. Gubler V, BJ Hoffman 1983. Asimple and very efficient method for generating cDNA libraries. Gene 25: 263-269. 16. Gurley WB, E Czarnecka, RT Nagao, JL Key 1986. Upstream sequences required for efficient expression of a soybean heat shock gene. Mol Cell Biol 6:559-565. 17. Heikkila JJ, JET Papp, GA Schultz, JD Bewley 1984. Induction of heat shock protein messenger RNA in maize mesocotyls by water stress, abscisic acid, and wounding. Plant Physiol 76:270-274. 18. Helm KW, NS Petersen, RH Abernethy 1989. Heat shock response of germinating embryos of wheat. Plant Physiol 90:598-605. 19. Hickey E, SE Brandon, R Poter, G Stein, J Stein, LA Weber 1986. Sequence and organization of genes encoding the human 27 kDa heat shock protein. Nucleic Acids Res 14:4127-4145. 20. Ingolia TD, EA Craic 1982. Four small Drosophila heat shock proteins are related to each other and to mammalian α-crystallin. Proc Natl Acad Sci USA 79:2360-2364. 21. Jinn TL, YC Yeh, YM Chen, CY Lin 1989. Stabilization of soluble proteins in vitro by heat shock proteins-enriched ammonium sulfate fraction from soybean seedlings. Plant Cell Physiol 30: 463-469. 22. Johnston RN, BL Kucey 1988. Competitive inhibition of hsp 70 gene expression causes thermosensitivity. Science 242:1551-1554. 23. Jones KA, RC Findly 1986. Indusion of heat shock proteins by canavanine in Tetrahymena. J Biol Chem 261:3703-3707. 24. Kelly PM, MJ Schlesinger 1978. The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblast. Cell 15:1277-1286. 25. Key JL, CY Lin, YM Chen 1981. Heat shock proteins of higher plants. Proc Natl Acad Sci USA 78:3526-3530. 26. Kloppstech K, G Meyer, G Schuster, IOhad. 1985. Synthesis, transport and localization of a nuclear encoded 22-kd heat-shock protein in the chloroplast membranes of peas and Chlamydomonas reinhardi. EMBO J 4:19012-1909. 27. Kozak M 1983. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev 47:1-45. 28. Krieg PA, DA Melton 1984. Functional messenger RNAs are produced by SP6 in vitro transcription of colned cDNAs. Nucleic Acids Res 12:7057-7070. 29. Krishnan M, HT Nguyen, JJ Bruke 1989. Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiol 90:140-145. 30. Kyte J, RF Doolittle 1982. A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105-132. 31. Leonard EE, E Czarbecjam JL Key 1986. Induction and accumulation of heat shock spectic poly(A+) RNAs and proteins in soybean seedlings during Arsenite and cadmium treatments. Plant Physiol 86: 1048-1056. 32. Li GC, A Laszlo 1985. Amino acid analogs while inducing heat shock proteins sensize CHO cells to thermal damage. J Cell Physiol 122:91-97. 33. Lin CY, JK Roberts, JL Key 1984. Acquisition of thermotolerance in soybean seedlings. Plant Physiol 74:152-160. 34. Lindquist S 1986. The heat-shock response. Ann Rev Biochem 55:1151-1191. 35. Lindquist S, EA Craig 1988. The heat-shock proteins. Ann Rev Genet 22:631-677. 36. Maniatis T, EF Fritsch, J Sambrook 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor NY. 37. Mans RJ, G Novelli 1961. Measurement of the incorporation of radioactive amino acids proteins by a filter paper disk method. Arch Biochem Biophys 94: 48-53. 38. Mansfield MA, JL Key 1987. Synthesis of the low molecular weight heat shock proteins in plants. Plant Physiol 84:1007-1017. 39. McDevitt MA, MJ Imperiale, H Ali, JR Nevins 1984. Requirement of a downstream sequence for generation of a poly(A) addition site. Cell 37: 993-999. 40. McElwain EF, S Spiker 1989. A wheat cDNA clone which is homologous to the 17kd heat-shock protein gene family of soybean. Nucleic Acids Res 17:1764. 41. Melton DA, PA Krieg, MR Rebagliati, T Maniatis, K Zinn, MR Green 1984. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res 12:7035-7056. 42. Murray EE, J Lotzer, M Eberle 1989. Codon usage in plant genes. Nucleic Acids Res 17:477-498. 43. Nagao RT, E Czarnecka, WB Gurley, F Sch?ffl, JL Key 1985. Genes for low-molecular-weight heat shock pro teins of soybean: Sequence analysis of a multigene family. Mol Cell Biol 5:3417-3428. 44. Nagao RT, JL Key 1989. Heat shock protein genes of plants. In: Cell Culture and Somatic Cell Genetics of Plants. Academic Press Vol 6, pp297-328. 45. O'Farrell PH 1975. High resolution two-dimentional electrophoresis of proteins. J Biol Chem 250:4007-4021. 46. Patricia ST 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77; 5201-5205. 47. Pelham HRB 1982. A regulatory upstream promoter element in the Drosophila HSP 70 heat-shock gene. Cell 30:517-528. 48. Raschke E, G Baumann, F Schoffl 1988. Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families. J Mol Biol 199:549-557. 49. Rougvie AE, JT Lis 1988. The RNA polymerase II molecule at the 5' end of the unduced hsp70 gene of D. melanogaster is transcriptionally engaged. Cell 54: 795-804. 50. Sanger F, S Nicklen, AR Coulson 1977. DNA sequencing with chain-termination inhibitors. Proc Natl Acad Sci USA 74: 5463-5467. 51. Sch?ffl F, E Rashke, RT Nagao 1984. The DNA sequence analysis of soybean heat-shock genes and identification of possible regulatory promoter elements. EMBO J 3:2491-2497. 52. Sch?ffl F, JL Key 1982. An analysis of mRNAs for a group of heat shock proteins of soybean using cloned cDNAs. J Molec Appl Genet 1:301-314. 53. Schuster G, D Even, K Kloppstech, I Ohad 1988. Evidence for protection by heat-shock proteins against photoinhibition during heat-shock. EMBO J 7:1-6. 54. Sorger PK, HRB Pelham 1988. Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation. Cell 54:855-864. 55. Southgate R, A Ayme, R Voellmy 1983. Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B. J Mol Biol 165:35-57. 56. Takahashi T, Y Komeda 1989. Characterization of two genes encoding small heat-shock proteins in Arabidopsis thaliana. Mol Gen Genet 219:365-372. 57. Vierling E, ML Mishkind, GW Schmidt, JL Key 1986. Specific heat shock proteins are transported into chloroplasts. Proc Natl Acad Sci USA 83:361-365. 58. Vierling E, RT Nagao, AE DeRocher, LM Harris 1988. A heat shock protein localized to chloroplasts is a member of a eukaryotic superfamily of heat shock proteins. EMBO J 7:575-581. 59. Voellmy R, D Rungger 1982. Transcription of a Drosophila heat shock gene is heat-induced in Xenopus oocytes. Proc Natl Acad Sci USA 79:1776-1780. 60. Wu CH, T Caspar, J Browse, S Lindquist, C Somerville 1988. Characterization of an HSP70 cognate gene family in Arabidopsis. Plant Physiol 88:731-740. 61. Xiao CM, JP Mascarenhas 1985. High temperature-induced thermotolerance in pollen tubes of Tradescantia and heat-shock proteins. Plant Physiol 78:887-890. 62. Yost HJ, S Lindquist 1986. RNA splicing is inter-rupted by heat shock and is rescued by heat shock protein synthesis. Cell 45:185-193. 63. Yost HJ, S Lindquist 1988. Translation of unspliced transcripts after heat shock. Science 242:1544-1548. 64. Zurfluh LL, TJ Guiltoyle 1982. Auxin-Induced changes in the population of translatable messenger RNA in elongating sections of soybean hypocotyls. Plant Physiol 69:332-337. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75748 | - |
dc.description.abstract | 以pCE53作為DNA探針(pCE53為大豆15-18kd低分子量熱休克蛋白質cDNA clone;由Dr. Joe L. Key, Department of Botany, the University of Georgia Athens, G.A. USA所贈),從水稻熱休克蛋白質cDNA庫中,共篩選到6個λclones。其中4個,cDNA inserts大小分別是0.5, 0.8, 0.7, 0.7kb。 以水稻RNA blot分析,顯示這幾個clones對水稻熱休克RNA (HS RNA)有專一性的雜合反應,而且這些雜合的熱休克RNA大略都是0.9kb。 這四個λ clones在次選殖(subcloned)到pGEM 3Z DNA之後,選擇了pTS1及pTS4 (cDNA inserts分別是0.8及0.5kb),進行hybrid-select in vitro translation以及cDNA定序等實驗。 pTS1及pTS4 hybrid-select in vitro translation的結果得到了同一群由7個蛋白質所組成的16-20kd的低分子量熱休克蛋白質,以及另一個30kd的熱休克蛋白質。 而pTS1及pTS4 cDNA序列比對的結果,彼此有70%的同源性(homology)。同時,這兩個clones,在DNA序列上,也與大豆LMW hsp class I基因有68%以上的同源性。 pTS1的胺基酸序列與GmHSP 18.5-C及Wheat C5-8分別有69.5%,80.4%的相同(idenity);而pTS1的hydropathy profile也顯示與其他已知的低分子量熱休克蛋白質有相似的圖形(pattern),不過pTS1的厭水性區域(hydrophobic domains)較多。 從以上的實驗結果,我們可以肯定pTS1及pTS4確實是水稻低分子量熱休克蛋白質cDNA clones。 | zh_TW |
dc.description.abstract | We have constructed a cDNA library from heat shocked rice seedlings; six HS spcific cDNA clones were isolated with pCE53 as a DNA probe (a low molecular weight soybean heat shock protein cDNA clone provided by Dr. Joe L. Key, Botany Dept., The Univ. of Georgia, Athens,GA. U.S.A.). The cDNA inserts of 4 λ clones were identified as 0.5, 0.8, 0.7, 0.7 kb long respectively; the Northern blot analysis indicated that these clones hybridized specifically to RNA from 41℃ treated rice seedlings,and no detectable signals appeared on RNA from controle (28℃) seedlings. The λ clones subsequently subcloned to pGEM 3Z were designated as pTS1, pTS2, pTS3, pTS4 (with 0.8, 0.7, 0.7, 0.5 kb cDNA inserts respectively). The hybrid-select in vitro translation of 41℃ poly(A)+RNA with pTS1 and pTS4,showed that these two clones hybrid-selected a group of HS poly(A)+ RNA,and translated to 7 peptides in the range of 16-20 kd, and one with 30 kd. The nucleotides of pTS4 and the full length cDNA clone -- pTS1 were sequenced with Sanger's dideoxynucleotide chain termination method, and the results showed 70% identity between these two cDNAs; also both clones have a high homology (above 68%) with soybean LMW hsp class I genes. The derived amino acid sequence of pTS1 showed 69.5% idenity with GmHSP 18.5-C and 80.4% idenity with wheat C 5-8 , the hydropathy profile of pTS1 showed a similar but more hydrophobic pattern with other LMW HSPs。 | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:06Z (GMT). No. of bitstreams: 0 Previous issue date: 1990 | en |
dc.description.tableofcontents | 中文摘要……………………………………………………I 英文摘要……………………………………………………II 前言……………………………………………………1 材料與方法……………………………………………………10 結果……………………………………………………34 圖表……………………………………………………43 討論……………………………………………………65 參考文獻……………………………………………………72 | |
dc.language.iso | zh-TW | |
dc.title | 水稻低分子量熱休克蛋白質cDNA clones之篩選與定序 | zh_TW |
dc.title | Screening and Sequencing of Rice Low Molecular Weight Heat Shock Proteins cDNA Clones | en |
dc.date.schoolyear | 78-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 78 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
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