請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75731完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林仲亮 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:59Z | - |
| dc.date.available | 2021-07-01T08:14:59Z | - |
| dc.date.issued | 1990 | |
| dc.identifier.citation | Andrews, P. (1965) The gel-filitraction behaviour of protein related to their molecular weights over a wide range. Biochem. J. 96: 959-960, Avruch, J.,and Wallach D. F. H. (1971) Preparation and properties of plasma membrane and endoplasmic reticulum fregments from isolated rat fat cells, Biochem. Biophys. Acta 233: 334-347. Bailyes, E. M. Luzio, J, P., and Newby, A. C. (1981) The use of a zwitterionic detergent in the solubilization and purification of the intrinsic membrane protein 5’-nucleotidase. Biochem. Soc. Trans. 9:140-141. Bailyes, E.M., Newby, A. C.,Siddle, K., and Luzio, J. P. (1982)Solubilization and purification of rat liver 5’-nucleotidase by use of a zwitterionic detergent and a monoclonal-antibody immunoadsorbent.Biochem. J. 203:245-251. Bailyes, E. M., Soos, M., Jackson, P., Newby A. C., Siddle K., and Luzio, J. P. (1984) The existence and properties of two dimers of rat liver ecto-5‘-nucleotidase. Biochem, J. 211: 369-377. Bailyes, E. M. Ferguson, M. A. J., Colaco, C. A. L. S., and Luzio, J, P. (1990) Inositol is a constituent of detergent-solubilized immunoaffinity-purified rat liver 5’-nucleotidase. Biochem. J. 265: 907-909. Baron, M. D., Pope, B., and Luzio, J. P. (1986) The membrane topography of ecto-5’-nucleotidase in rat hepatocytes. Biochem, J. 236: 495-502. Barons M. D. and Luzio, J. P. (1987) The synthesis and turnover of 5’-nucleotidase in primary cultured hepatocytes. Biochem. Biophys, Acta 927: 81-85. Bontemps, F., Vincent, M. F. Van den Bergh, F. van Waeg, G. and Van den Berghe, G. (1989) Stimulation by glycerate 2,3-biphosphate: a common property of cytosolic IMP-GMP 5‘-nucleotidase in rat and human tissues. Biochem. Biophys. Acta 997: 131-134. van den Bosch, R. A. du Maine, A. P. M., Geuze, H, J., van der Ende, A., and Strous, C. J. (1988) Recycling of 5’-nucleotidase in a rat hepatoma cell line. EMBO J. 7 3345-3351. Bushchette-Brambrink, B., and Gutensohn, W. (1989) Human plancental ecto-5‘-nucleotidase isoforms and chemical crosslinking products of membrane-bound and isolated enzyme. Biol. Chem. Hoppe-Seyler, 370: 65-74. Cammer, W.,and Tansey F. A. (1986) 5’-Nucleotidase localization in the brains and spinal cords of adult normal and dysmyelinating mutant (shlverer) mice. J. Neurol. Sci 73: 299-310. Casad V., Mallol, J., and Bozal, J. (1988) Localization of 5’-nucleotidase in bovine brain myelin fraction and myelin subfraction. Neurochem. Res. 13: 359-368. Centelles, J, J., Franco, R. and Canela, E. I. (1986) Kinetics of the 5’-nucleotidase nad the adenosine deaminase in subcellular fractions of rat brain. Neurochem. Res. 11: 471-479, Chrambach, A. Jovin, T. M., Svendsen, P. J. and Rodbard, D. (1976) in Methods of Protein Separation (Catsimpoolase, N. ed.) Vol.2 pp. 27-144. Plenum Press, New York. Chuang,N.-N. Newby,A. C. and Luzio J. P. (1984) Characterization of different molecular forms of 5’-nucleotidase in normal serum and in serum from cholestatic patients and bile-duct-ligated rats. Biochem. J. 224: 689-695. DePierre, J,W., and Karnovsky, M. L. (1973) Plasma membranes of mammalian cells. J. Cell Biol 56: 275-303. Dixon, M.,Webb, E. C., Thorne, C. J. R.9 and Tipton K. F. (1979) in Enzymes. 3rd ed.pp. 47-206 Longmans,Green & Co. London and Academic Press, New York. Dornand, J., Bonnafous, J.-C.,and Mani, J.-C. (1978)Purification and properties of 5’-nucleotidase from lymphocyte plasma membranes. Eur. J. Biochem. 87: 459-465. Emmelot, P., Bos, C. J., Benedetti E. L., and Rumke, P. H. (1964) Studies on plasma membranes. Biochem. Biophys. Acta 90: 126-145. Fastbom, J., Pazos, A. and Palacios, J. M. (1987) The distribution of adenosine Al receptors and 5‘-nucleotidase in the brain of some commonly used experimental animals. Neuroscience 22: 813-826. Ferguson, K.A. (1964) Starch-gel electrophoresis-application to the classification of pituitary proteins and polypeptides. Metabolism 13: 985-1002. Fijisawa,K. and Yoshino, M. (1987) Activities of adenylate-degrading enzymes in muscles from vertebrates and invertebrates. Comp. Biochem. Physiol. 86B: 109-112. Gutensohn, W.,and Jahn, H. (1988) Qualitative and quantitative differences between burkitt lymphoma and lymphoblastoid cell lines in expression of membrane ecto-nucleotidases and in the response to againsts of the A2 type adenosine receptor. In J.Cancer 41:436-441. Hedrick,J. L.,and Smith, A. J. (1968) Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrohoresis. Arch. Biochem. Biophys. 126:155-164. Henke,W, Lang, M., Dubiel, W., Holzhutter, H.-G., and Gerber C. (1989) Identification and characteristics of a norvel mitochondrial 5’-nucleotidase in rat liver. Biochem. Int. 181:833-844. Heymann, D.Reddington, M.and Kreutzberg, C. W. (1984) Subcellular localization of 5’-nucleotidase in rat brain. J. Neurichem. 43: 971-978. Itami,H.,Sakai,Y., Shimamoto, T. Hama, H. Tsuda, M., and Tsuchiya, T. (1989) Purification and characterization of membrane-bound 5’-nucleotidase of Vibro paraemolyticus. J. Biochem. 105: 785-789. Lachant, N. A., Zerez, C, R. and Tanaka, K. R. (1989) Pyrimidine nucleotidase impair phosphoribosylpyrophosphate (PRPP) synthetase subunit aggregation by sequestering magnesium. Biochem. Biophys. Acta 994: 81-88. Laemmli, U.K.(1970)Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. Lanes L. C. (1978) A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis. Anal Biochem. 86: 655-664. Le Hir, M., Gandhi, R., and Dubach, U. C. (1989) Purification and properties of a 5’-nucleotidase from rat renal membranes, Enzyme 41: 87-93. Low, M.G., and Prasad, A. R. S. (1988) A phospholipase D specific for the phosphatidyalinositol anchor of cellsurface proteins is abundant in plasma. Proc. Natl. Acad. Sci. USA 85: 980-984. Low,M.(1989) Glycerosyl-phosphatidyalinositol: a versatile anchor for cell surface proteins. FASEB 3: 1600-1608. Lowery, O, H. Rosebrough, N. J., Farr A. L. and Randall R. J, (1951) Protein measurement with the folio phenol reagent. J. Biol. Chem. 193: 265-275. Maguire, G. A. and Luzio, J. P. (1984) The presence and orientation of ecto-5’-nucleotidase in rat liver lysosomes FEBS Letter 180: 122-126. McCormick, A., Miless L. E. M. and Chrambach A. (1976) Selective elution of zones form preparative isoelectric focusing gels by ampholytes or buffers. Anal. Biochem. 75:314-324. Naito,Y., and Lowenstein, M. (1981) 5’-Nucleotidase from rat heart. Biochemistry 20: 5188-5194. Nakamura S.(1976) Effect of sodium deoxycholate on 5’-nucleotidase. Biochem. Biophys. Acta 426: 339-347. Newby, A. C., Luzio, J. P. and Hales, C. N. (1975) The properties and extracellular location of 5’-nucleotidase of the rat fat-cell plasma membrane. Biochem. J. 146: 625-633. Newby, A. C., Rodbell M., and Charmbach, A. (1978) Adenylate cyclase in polyacrylamode gel electrophoresis: solubilized but active. Arch. Biochem. Biophys. 190: 109-117. Richardson, P. J, Brown, S. J., Bailyes, E. M., and Luzio, J. P. (1987) Ectoenzymes control adenosine modulation of immunoisolated cholinergic synapases. Nature 327: 232-234. Risse, C., Stochaj, U., Elasser, K.,H.C. and von der Mark. K. (1989) the 68 kDa lamninin-binding protein chicken muscular sources: evidance against a gross structural similarity of both proteins. Biochem. Biophys. Acta 994:258-263. Rodbard, D., Chrambach, A. (1971) Estimation of molecular radius, free mobility, and valence using polyacrylamide gel electrophoresis. Anal. Biochem. 40: 95-134. Senesi, S., Freer, C., Gasperini, M., Agioni M. R., and Nario, C. (1987)Mouse lymphocyte enzymatic marker: a rapid method for achieving selective solubilization and efficient recovery of the membrane-bound 5’-nucleotidase. J. Immunol. Methods 100: 197-209. Spychala, J.,and Makarewicz, W. (1987) Adenosine-5’-monophosphate catabolism in frog liver. Comp. Biochem. Physiol. 81B: 481-483. Stanley, K. K., Edwadrs, M. R., and Luzio. J. P. (1980) Subcellular distribution and movement of 5’-nucleotidase in rat cells, Biochem. J. 186: 59-69. Switzer, R, C., Merril, C, R., and Shifrin, S, (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 98: 231-237. Thomopson, L. F., Ruedi, J. M., Low, M. C., and Clement L. T. (1987) Distribution of ecto-5’-nucleotidase on subset of human T and B lymphocytes as detected by indirect immunofluoresence using goat antibodies. J. Immunol. 139:4042-4048. Truong, V. L., Collinson, A. R., and Lowe n stein, J. M. (1988) 5’-Nucleotidase in rat heart: evidance for the occurrence of two soluble enzymes with different substrate specificities. Biochem. J, 253: 117-121. Wada, I., Eto, S., Himeno, M. and Kato, K. (1987) 5‘-nucleotidase in rat liver lysosome. J, Biochem., 101:1077-1085. Wada, I., Himeno, M., Furuno, K. and Kato, K. (1986) Biosynthesis and intracellular transport of rat liver 5’-nucleotidase. J. Biol. Chem. 261: 2222-2227. Widnell, C. C.,Schneider, Y.-J.,Pierre, B. Baudhuin P. and Trouet, A. (1982) Evidence for a continual exchange of 5’-nucleotidase between the cell surface and cytoplasmic membranes in cultured rat fibroblasts. 26: 61-70. Zekri, M., Harb, J., Bernard S., and Meflah, K. (1988) Purification of bovine liver cytosolic 5’-nucleotidase kinetic and structural studies as compared to the membrane isoenzyme. Eur. J. Biochem. 172: 93-99. Zerki, M. Harb, J., Bernard, S., Poirier, C., Devaux, C., and Meflah, K. (1989) Differences in the release of 5‘-nucleotidase and alkaline phosphatase from plasma membrane of several cell types by PI-PLC. Comp, Biochem. Physiol. 93B:673-679. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75731 | - |
| dc.description.abstract | 五核甘酸?(5’-nucleotidase, 5'NT)是一種細胞膜上的標記酵素(Marker enzyme)，廣泛存在於細胞膜上，其主要的作用機制是將五腺核甘單磷酸(Adenosine 5’-monophosphate)水解成腺嘌呤(Adenosine)和單磷酸(monophosphate)。本實驗是利用介面活性劑Sulphobetaine14 (SB-14)來萃取並純化腦細胞膜上的五核甘酸?，利用兩支以100mM和10mM Tris所平衡的DE-52陰離子交換管柱(Anion exchanger colume)和一支AcA-34膠體過濾管柱(Gel filitraction colume)進行酵素純化。 經過純化所得到的腦的五核甘酸?以SDS-PAGE、自然膠體電泳(Native gel electrophoresis)、和膠體過濾法(Gel filitraction chromatograph)等方法所測得的分子量均在65,000-75,000 Dalton之間，約是同時純化得到的肝臟細胞膜的五核甘酸?(其分子量?150,000 Dalton)的一半。因?肝臟的五核甘酸??雙原分子，故推測腦的五核甘酸?應以單原分子形式存在。 此外，在比較這兩種不同組織的酵素性質時，可發現兩者有很大的差別。例如：腦的5'NT的Km值?12μM，肝臟?20μM；而腦的5'NT的Vmax則?82nm Pi/min，肝臟?151 nm Pi/min。比較兩者的等電點(Isoelectric point, pI)時發現：腦的5'NT的pI值在6.5到8.0之間，而肝臟的5'NT的pI則在4.8到5.2之間。但兩者有相同的作用酸鹼值(Optimal pH)?7.5，並皆可?磷脂?D所作用，表示兩者都是以聚醣磷脂醯肌醇(Glycosyl-phosph-atidylinositol, GPI)接合於細胞膜上。 | zh_TW |
| dc.description.abstract | 5’-Nucleotidase (5’NT, EC 3.1.3.5) was extracted from the rat brain membrane by using the zwitterionic Sulphobetaine 14 (SB-14). After two ion-exchange filitraction chrpmatpgraphies the 5’NT was purified with a final specific activity of 8.25 units/mg of protein. Based on the estimations by gel filitraction, native gel electrophoresis sand SDS-PAGE, I concluded the enzyme with a molecular weight of 65 kDa-75 kDa, Its isoelectric point is between 6.5-8.0. The enzyme has a Km value of 12 μM by using 5‘AMP as substrate, and has an optimal enzyme activity at pH 7.5. The treatment with phospholipase D could shift the enzyme to a more basic posit ion in the native gel electrophoresis system, It is concluded that the 5’-nucleotidase is anchored on the membrane of brain with the glycosyl-phosphatidylinositol (GPI). | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:59Z (GMT). No. of bitstreams: 0 Previous issue date: 1990 | en |
| dc.description.tableofcontents | 圖目錄. . . . . . . . . . . . . . . . . ii 表目錄. . . . . . . . . . . . . . . . . iv 摘要. . . . . . . . . . . . . . . . . l 一、導言. . . . . . . . . . . . . . . . . . 2 二、材料與方法. . . . . . . . . . . . . . . 5 三、結果. . . . . . . . . . . . . . . . . . l2 四、討論. . . . . . . . . . . . . . . . . . 18 五、參考文獻. . . . . . . . . . . . . . . . 21 六、圖表. . . . . . . . . . . . . . . . . . 29 七、英文摘要. . . . . . . . . . . . . . . . 63 八、謝辭. . . . . . . . . . . . . . . . . 64 | |
| dc.language.iso | zh-TW | |
| dc.title | 純化與定性大白鼠腦細胞膜的五核甘酸? | zh_TW |
| dc.title | Purification and Characterization of 5'-Nucleotidase from the Plasma Membrane of Rat Brain | en |
| dc.date.schoolyear | 78-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 64 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。