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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | HSUEH-KUAN LU | en |
dc.contributor.author | 呂學冠 | zh_TW |
dc.date.accessioned | 2021-07-01T08:14:53Z | - |
dc.date.available | 2021-07-01T08:14:53Z | - |
dc.date.issued | 1989 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75718 | - |
dc.description.abstract | 研究中為獲得淨化的藻體,採用的方法包括化學方法如次氯酸鈉,酒精等滅菌處理,物理方法如超音波振盪、紫外線照射等,以達到無菌,無雜藻污染的藻體,做為生物技術研究所使用的材料。 綠藻類如石蒪屬Ulva、石髮屬Enteromorpha之原生質體誘導與釋放僅需纖維素?,而誘導紅藻龍鬚菜屬Gracilaria原生質體釋放則需以九孔消化腺抽出物為主,添加纖維素?、果膠?及粗多醣分解?;本省養殖種龍鬚菜G.tenuistipitata var.liue 其原生質體之釋放條件如酵素組成、溫度、酸?度、離子濃度、時間及前暗處理等均加以比較,其最高的釋放率可達5.8×10^5 cells/ml/0.1g藻體;其他如石蒪(Ulva sp.)、石?(Enteromorpha spp.)紫菜(Porphyra spp.)等亦可達1×10^4 cells/ml/0.1g藻體以上。 石?Enteromorpha compressa原生質體培養於 P E S I 培養液中,可再生成癒合組織,並在無生長激素添加下,由癒合組織分化成為小株藻體;癒合組織與小株藻體之部份細胞,均可能再釋放出小型孢子,這些小型孢子可能長大萌發成小株藻體或再次放出小孢子。紫菜、龍鬚菜原生質體的培養尚待進一步之努力。 原生質體融合方面,曾嚐試紫菜Porphyra angusta 與石蒪Ulva pertusa 原生質體異種間融合,但並沒有成功,然石蒪Ulva pertusa原生質體同種間融合則可在37.5%的聚乙二醇(PEG 1500)的溶液中誘導原生質體互相靠近,並在鹼性溶液中含高濃度 (0.1M)鈣離子激發的情況下,相接觸的膜面破裂而促使兩細胞融合。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:53Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
dc.description.tableofcontents | I 、前言………………………………………1 II 、材料與方法…………………………………6 A 、一般材料方法 ……………………………6 1 .藻體與無菌培養…………………………6 2 .酵素的來源及製備………………………7 3 .原生質體定量………………………………8 4 .原生質體鑑定……………………………9 B 、原生質體釋放條件…………………………10 1 .酵素來源及組成……………………11 2 .海水及鎂、鈣離子濃度……………12 3 .藻重、藻體與藻種…………………12 4 .溫度、酸鹼度與時間………………13 5 .前暗處理………………………………14 C 、原生質體培養…………………………14 1.石?Enteromorpha compressa……………14 2.龍鬚菜Gracilaria tenuistipitata var lieu……14 D 、原生質體融合………………………15 III、結果與討論………………………15 A 、藻體與無菌處理…………………16 B 、原生質體鑑定……………………17 1 .活性…………………………………17 2 .細胞壁………………………………19 C 、原生質體釋放條件…………………19 1 .酵素來源及組成…………………19 2 .海水與鎂、鈣離子濃度……………22 3 .藻重、藻體與藻種…………………24 4 .溫度、酸鹼度與時間 …………25 5 .前暗處理………………………27 D 、原生質體培養…………………28 1 .石髮 Enteromorpha…………28 2 .龍鬚菜 Gracilaria…………29 E 、原生質體融合……………30 W 、結論………………………33 V 、參考文獻……………………35 VI 、表格…………………………45 VII、圖型…………………………60 VIII、附錄………………………78 | |
dc.language.iso | zh-TW | |
dc.title | 海藻原生質體:誘發、分離、培養及融合之研究 | zh_TW |
dc.title | STUDIES OF SEAWEED PROTOPLAST:INDUCTION, ISOLATION, CULTURE AND FUSION | en |
dc.date.schoolyear | 77-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 93 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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