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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.author | 蕭悅臣 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:50Z | - |
| dc.date.available | 2021-07-01T08:14:50Z | - |
| dc.date.issued | 1989 | |
| dc.identifier.citation | 1.Schaubert,D.,Heinemann,S.,Carlisle,W.Tarikas,H.,Kimes,B.,Patrick,J.,Steinbach,H.,Culp,W. and Brandt,B. L. (1974).Clonal cell lines from the rat central nervous system. Nature. 249,224-227. 2. Shih,C.,Padhy,L. C.,Murray,M. and Weinberg,R. A.(1981).Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature. 290,261-264. 3. Gorman,C.,Padmanabhan,R. and Howard,B. H. (1983)High efficiency DNA-mediated transformation of Primate cells. Science. 221,551-553. 4. Schechter,A. L.,Stern,D. F.,Vaidyanathan,L.,Decker,S. J.,Drebin,J. A.,Greene,M. I. and Weinberg,R. A.(1984).The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature. 312,513-516. 5. Shechter A. L.,Hung,M. C.,Coussens,L. (1985). The neu gene: an erbB-homologous gene distinct from and unlinked to the gene encoding the EGF receptor. Science. 229,976-978. 6. Hung,M. C.,Schechter,A. L.,Vaidyanathan,L.,Stern,D. F. and Weinberg,R. A. (1986). Isolation of the molecular clone of the neu oncogene from the B103 rat neuro/glioblastoma cell line.BMEC.391-395. 7. Hung, M. C., Schechter,A. L., Chevray,P-Y, M., Stern D. F. and Weinberg,R. A. (1986).Molecular cloning of the neu gene: absence of gross structural alteration in oncogenic alleles. Proc. Natl. Acad. Sci. USA. 83,261-264. 8. Bargmann,C. I.,Hung,M-C. and Weinberg,R. G.(1986).The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature. 319,226-230. 9.Yamamoto,T.,Ikawa,S.,Akiyama,T.,Semba,K.,Nomura,N.,Miyajima,N.,Saito,T. and Toyoshima,K. (1986).Similarity of protein encoded by the human c-erb B-2 gene to epidermal growth factor receptor. Nature. 319,230-234. 10. Maniatis T,Fritsch E,Sambrook J: “Molecular Cloing,A Laboratory Manual.Cold Spring Harbor NY: Cold spring Harbor Labortary,1982. 11. Kokai,Y.,Cohen,J. A.,Derbin,J. A. and Geene,M. I. (1987). Stage- and tissue-specific expression of neu oncogene in rat development.Proc. Natl. Acad. Sci. USA. 84,8498-8501. 12. Welder,J. (1988).Antisense DNA and RNA: progress and prospects. Genes & Development. 2,502-504. 13. Green,N. R. (1989).When the products of oncogenes and anti-oncogenes meet.Cell. 56,1-3. 14. Hudziak,R. M.,Schlessinger,J. and Ullrich,A. (1987) Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells. Proc. Natl. Aced. Sci. USA. 84,7159-7163. 16. Spiegelman,B. M.,Frank,N. and Green,H.(1983).Molecular cloning of mRNA from 3T3 adipocytes. J. B. C. 258,10083-10089. 17. Stuart G. W.,Searle,P. F.,Chen,H. Y.,Brinster,R. L. and Palmiter,R. D. (1984). A 12-base-pair DNA motif tjat is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene. Proc. Natl. Acad. Sci. USA. 81,7318-7322. 18. Davis,L. G.,Dibner,M. D. and Battey,J. F. (1986).Basic methods in molecular biology. 19. Melton,D. A. (1985).Injected anti-sense RNAs specifically block messenger RNA translation in vivo. Proc. Natl. Aced. Sci. USA. 82,144-148. 20. Yokoyama,K. and Imainoto,F. (1987). Transcriptional control of the endogenous MYC protooncogene by antisense RNA.Proc. Natl. Acad. Sci. USA. 84,7363-7367. 21. Holt,J. T.,T. V. Gopal,A. D. Moulton,and A.W. Nienhuis. (1986).Inducible production of c-fos anti-sense RNA inhibits 3T3 cell proliferation.Proc. Natl. Aced. Sci. USA. 83,4794-4798. 22. Nishikura,K.,and J. M. Murray. (1987). Antisense RNA of proto-oncogene c-fos blocks renewed growth of quiescent 3T3 cells. Mol. Cell. Biol.7,639-649. 23. Wickstrom ,E. L.,T. A. Bacon,A. Gonzalez.,D.L. Freeman.,G. H. Lyman.,and E. Wickstrom.(1988).Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhihted by an antisense penladecadeoxynucleolidet targeted against c-myc mRNA.Proc. Natl. Acad. Sci. USA. 85,1028-1032. 24. Thomposon, T. C.,J. Southgate., G. Kitchener, and H. Land. (1989).Multistage carcinogenesis induced by ras and myc oncogenes in a reconstituted organ. Cell. 56,917-930. 25. Helmut. (1987).Revertants of v-fos-transformed fibroblasts have mutations in cellular genes essential for transformation by other oncogenes.Cell. 51,357-369. 26. Bargmann,C. I.,and Weinberg,R. A. (1988). Oncogenic activation of the neu-encoded receptor protein by point mutation and deletion.EMBO. 7,2043-2052. 27. Ratner,M. (1989.).Can the antisense message be delivered Bio/Technology. 7,207. 28. Weiner,D. B.,jing,L.,Cohen,J. A.,Geene,M. I.(1989).A point mutation in the neu oncogene mimics ligand induction of receptor aggregation. Nature. 339,230-234. 29. Kokai,Y.,Bobashi,K.,Geene,M.(1988). Phosphorylation process induced by epidermal growth factor alters the oncogenic and cellular neu (NGL) gene products.Proc. Natl.. Acad. Sci. USA. 85,5389-5393. 30. Slamon,D. J.,Clark,G. M.,wong,S. G.(1987). Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science. 235,943-945. 31. Slamon,D. J.(1989).Studies of the HER-2/neu Proto-oncogene in Human Breast arid Ovarian Cancer. Science. 244,707-712. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75711 | - |
| dc.description.abstract | 目前對癌症的研究，已知癌細胞的形成和oncogene有密切的關係，本實驗以B104-1 cell為model利用Antisense RNA techingue去抑制癌細胞的oncogene功能，以期瞭解是否可將癌化細胞轉變為正常的纖維細胞（NIH3T3) ．進一步更以Native癌細胞作為觀察對象，是否也能經由抑制其oncogene的功能而轉化為正常細胞． 我們先construct antisense DNA vector，再將antisense vector送入癌細胞，挑選其transfectonts．由southern blot analysis知道Antisense DNA已插入癌細胞的Genomic DNA上．將挑選到的transfectants 做in vitro Biologioal assay(Soft agar assay) ，發現有partially revert現象，另外又發現一種類似revertant cell line．進一步以Native cancer cell做soft agar assay，也發現有partially revert甚至有些transfectant有fully revert回normal cell的現象． | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
| dc.description.tableofcontents | 一．中文摘要………………1 二．緒言……………………2 三. 實驗過程簡圖………………7 四．實驗步驟…………………8 五．實驗材枓，試劑和儀器……………13 六．結果…………………18 七．討論………………………32 八. 參考文獻………………………35 九．謝辭………………………………38 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用反順序核糖核酸技術將癌化的癌細胞轉變?正常的細胞 | zh_TW |
| dc.title | Reversion of Cancer Cells to Normal Cells By Antisense-Oncogene | en |
| dc.date.schoolyear | 77-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 41 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| Appears in Collections: | 生化科學研究所 | |
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