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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75682完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 溫秋明 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:39Z | - |
| dc.date.available | 2021-07-01T08:14:39Z | - |
| dc.date.issued | 1989 | |
| dc.identifier.citation | 1. 王聖予 (1985) 大肚魚細胞株之建立及其生物學特性之研究，國立臺灣大學動物學研究所碩士論文。 2. 周西平 (1988) 鎘對魚類細胞株和哺乳類細胞之作用，國立臺灣大學動物學研究所碩士論文。 3. Aaronson, S.A. & Todaro, G.J. (1968) Basis for the definition of malignant potential by mous cells cultivated in vitro. Science 162: 1024-102 4. Adams, R.L.P. (1980) Cell culture for biochmists. Vork, T.S. and Burdon, R.H. eds. Elsevier / North-Halland, Biomedical Press. 5. Ahne (1979) Fish cell culture : a fibroblast line (PG) from ovaries of juvenile pike (Esox lucius). In. Vitro 15: 839-840. 6. Balls, M. & Worley, R.S. (1973) Amphiban cells in vitro. II. Effects of variations in medium osmolarity on a permanent cell line isolated from xenopus. Exp. Cell Res. 76: 333-336. 7. Bartholomew, J.C., Neff, N.T. & Ross, P.A. (1976) Stimulation of WI-38 cell cycle transit : effect of serum concentration and cell density. J. Cell. Physiol. 89: 251-258. 8. Bibor-Hardy, V. & Bernard, M. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75682 | - |
| dc.description.abstract | 本實驗利用添加20%-10%胎牛血清的L-15培養基建立了花身雞魚(Therapon jarbua FORSK?L)胸鰭來源的永久性細胞株(JF)，並針對此細胞株做各種特性的研究。 此株化細胞經顯微，超微及染色等觀察顯示，細胞形態兼具纖維狀及表皮狀。細胞在基質上貼平鋪展，細胞表面平滑，鮮有微絨毛等突起。細胞間有絲狀或葉狀偽足接觸。細胞內，細胞核呈不規則形，粒線體，內質網及高氏體均很發達，柱狀的粒線體以近核區為中心散射，內質網內含有許多合成物質，顯示細胞的代謝功能或分泌功能都很旺盛。各種液態培養環境中，JF細胞株最適合的環境下，其細胞數目倍增的時間約2天。pH 6-8或滲透壓200 mmol/Kg-650 mmol/Kg的條件下細胞均可生長。以pH 6.5-7.5和滲透壓450 mmol/Kg左右的環境下最適合生長。不同的pH，滲透壓，血清濃度和培養溫度對細胞的生長速率，附著效率都有影響。細胞的大小會隨pH增高而增大，在高溫(37℃)培養時細胞也會變大。pH及參透壓過高或過低均會造成細胞病變。染色體數目的分佈從10幾條至90幾條都有而具48條的細胞最多。群落形成率在1.7%以下。 由實驗結果顯示此株化細胞具停泊依賴性，血清依賴性密度，依賴性等正常細胞的特性。對於Infectious Pancreas Necrosis Virus (IPNV) 病毒AB, VR-299, Sp等株及CSV病毒均有感受性，平均每個細胞的病毒產量IPNV約420-1327 TCIDoo/cell, CSV約9 TCIDoo/cell。細胞在液態氮中貯存8個月仍有95%的回收率，且尚未被細菌、黴菌、黴漿菌或病毒污染。本細胞株適合細胞生物學，病毒學或其他方面的研究。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:39Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
| dc.description.tableofcontents | 摘 要一一一一一一一一一一一一一一一一一一一Ⅰ 誌 謝一一一一一一一一一一一一一一一一一一一Ⅱ 目 錄一一一一一一一一一一一一一一一一一一一Ⅲ 第一章 緒論一一一一一一一一一一一一一一一一1 第二章 材料及方法一一一一一一一一一一一一一5 第一節 材 料一一一一一一一一一一一一一一一一5 第二節 方 法一一一一一一一一一一一一一一一一5 2.1 建立細胞株一一一一一一一一一一一一一一一5 2.1-1 初級培養－一一一一一一一一一一一一一一5 2.1-2 繼代培養一一一一一一一一一一一一一一一 5 2.2 細胞形態的觀察一一一一一一一一一一一一一6 2.2-1 顯微觀察一一一一一一一一一一一一一一一6 2.2-2 超微觀察一一一一一一一一一一一一一一一6 2.3 染色體及其數目分佈的觀察一一一一一一一一7 2.4 生長曲線的測定一一一一一一一一一一一一一8 2.5 環境因數對細胞的影響一一一一一一一一一一8 2.5-1 溫度對細胞的影響一一一一一一一一一一一 8 2.5-2 pH值對細胞的影響一一一一一一一一一一一9 2.5-3 血清濃度對細胞的影響一一一一一一一一一10 2.5-4 滲透壓對細胞的影響一一一一一一一一一一11 2.6 細胞群落形成率一一一一一一一一一一一一一11 2.7 病毒實驗一一一一一一一一一一一一一一一一12 2.7-1 感受病毒的測試一一一一一一一一一一一一12 2.7-2 病毒效價的測定一一一一一一一一一一一一12 2.7-3 病毒產量的測定一一一一一一一一一一一一12 2.7-4 細胞病變的觀察一一一一一一一一一一一一13 2.8 長期保存一一一一一一一一一一一一一一一一13 2.8-1 細胞冷凍一一一一一一一一一一一一一一一13 2.8-2 細胞解凍及存活率一一一一一一一一一一一14 2.9 污染檢驗一一一一一一一一一一一一一一一一14 2.9-1 細菌檢驗一一一一一一一一一一一一一一一15 2.9-2 黴菌檢驗一一一一一一一一一一一一一一一15 2.9-3 黴漿菌檢測一一一一一一一一一一一一一一15 第三章 結果一一一一一一一一一一一一一一一一16 3.1 細胞株一一一一一一一一一一一一一一一一一16 3.1-1 初級細胞株一一一一一一一一一一一一一一16 3.1-2 永久細胞株一一一一一一一一一一一一一一16 3.2 細胞形態一一一一一一一一一一一一一一一一17 3.3 染色體的形態及數目的分佈一一一一一一一一18 3.4 生長曲線一一一一一一一一一一一一一一一一18 3.5 環境因數對細胞的影響一一一一一一一一一一18 3.5-1 溫度的影響一一一一一一一一一一一一一一18 3.5-2 pH值的影響一一一一一一一一一一一一一一19 3.5-3 血清濃度的影響一一一一一一一一一一一一20 3.5-4 滲透壓的影響一一一一一一一一一一一一一20 3.6 細胞群落形成率一一一一一一一一一一一一一20 3.7 病毒的感受範圍 產量及造成的病變一一一一一21 3.8 細胞的保存一一一一一一一一一一一一一一一21 3.9 污染鑑定一一一一一一一一一一一一一一一一22 第四章 討論一一一一一一一一一一一一一一一一 23 附 錄一一一一一一一一一一一一一一一一一一一一29 參考文獻一一一一一一一一一一一一一一一一一一34 圖 表一一一一一一一一一一一一一一一一一一一一41 | |
| dc.language.iso | zh-TW | |
| dc.title | 花身雞魚(Therapon jarbua FORSK?L)鰭細胞株之建立及其生物學特性研究 | zh_TW |
| dc.title | The Biological Characteristics of A Cell Line Derived from Jarbua (Therapon jarbua FORSK?L) Fin | en |
| dc.date.schoolyear | 77-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 74 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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