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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Hsien-Jung Chen | en |
| dc.contributor.author | 陳顯榮 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:25Z | - |
| dc.date.available | 2021-07-01T08:14:25Z | - |
| dc.date.issued | 1988 | |
| dc.identifier.citation | 1. Belser, W. L., J. B. Murphy, D. P. Delmer and S. E. Mill. 1971 End product control of tryptophan biosynthesis in extracts and intact cells of the higher plant Nicotiana tabacum var Wisconsin 38.Biochim. Biophys. Acta 237:1-10. 2. Bickel, H., L. Palme and G. Schultz. 1978 Incorporation of shikimate and other precursors into aromatic amino acid and phenylquinones of isolated spinach chloroplasts. Phytochemistry 17:119-124. 3. Bourgin, J. P., J. Goujaud, C. Missonier and C. Pethe. 1985 Valine-resistance, a potential marker in plant cell genetics. 1. Distinction between two types of valine-resistant tobaco mutants isolated from protoplast-derived cells. Genetics 109:393-407. 4. Bright, S. W. J., J. S. H. Kueh and S. E. Rognes. 1983 Lysine transport in two barley mutants with altered uptake of basic amino acids in the root. Plant Physiol.72:821-824. 5. Bright, S. W. J., P. B. Norbury and B. J. Miflin. 1979 Isolation of a recessive barley mutant resistant to S-(2-aminoethyl)L-cysteine. Theor. Appl. Genet.55:1-4. 6. Brotherton, J. E., R. M. Hauptman and J. M. Widholm. 1986 Anthranilate synthase forms in plants and cultured cells of Nicotiana tabacum L. Planta 168:214-221. 7. Buchholz, B., B. Reupke, H. Bickel and G. Schultz. 1979 Reconstitution of amino acid synthesis by combining spinach chloroplasts with other leaf organelles. Phytochemistry 18:1109-1111. 8. Carlson, J.E. and J. M. Widholm. 1978 Separation of two forms of anthranilate synthase from 5-methyltryptophan-susceptible and resistant cultured Solanum tuberosum cells. Physiol. Plant. 44:251-255. 9. Cella, R. and P. Iadarola. 1983 Characterization of carrot cell lines resistant to 5-methyltryptophan obtained by irradiating suspension cultures with UV-light. Plant Sci. Lett.29:327-337. 10. Chen, C.C., C.R. Huang and K. Y. To. 1985 Anther cultures of four diploid Nicotiana species and chromosome numbers of regenerated plants. Bot. Bull. Acad. Sin.26:147-153. 11. Cook, A. R. and R. L. Bieleski. 1969 Fractionation of plant extracts by thin-layer electrophoretic and chromatographic procedures. Anal. Biochem. 28:428-435. 12. Curtiss, C. D., J. M. Widholm and R. A. Gonzales. 1987 Selection and characterization of methionine and tryptophan analog resistant Asparagus cells. J. Plant Physiol. 138:125-135. 13. d'Amato, T. A., R. J. Ganson, C. G. Gaines and R. A. Jensen. 1984 Subcellular localization of chorismate-mutase isozymes in protoplasts from mesophyll and suspension-cultured cells of Nicotiana Sylvestris. Planta 162:104-108. 14. Demoss, J. A. 1965 The conversion of shikimic acid to anthranilic acid by extracts of Neurospora crassa. J. Biol. Chem.248:1231-1235 15. Goers, S. K. and R. A. Jensen. 1984a Separation and characterization of two chorismate-mutase isozymes from Nicotiana sylvestris. Planta 162:109-116. 16. Goers, S. K. and R. A. Jensen. 1984b The differential allosteric regulation of two chorismate-mutase isozymes from Nicotiana sylvestris. Planta 162:117-124. 17. Huang, H.C. and C.C. Chen. 1988 Genome multiplication in cultured protoplasts of two Nicotiana species. J. Hered. 79:28-32. 18. Jacobsen, E., R. G. F. Visser and J. Wijbrandi. 1985 Phenylalanine and tyrosine accumulating cell lines of a diploid potato selected by resistance to 5-methyltryptophan. Plant Cell Reports 4:151-154. 19. Lien, R. and S. E. Rognes. 1977 Uptake of amino acids by barley leaf slices: kinetics, specificity, and energetics. Physiol. Plant. 41:175-183. 20. Maliga, P. 1982 Cell culture procedures for Nicotiana plumbaginifolia PMB Newsletter 3:88-94. 21. Moyed, H. S. 1960 False feedback inhibition: inhibition of tryptophan biosynthesis by 5-methyltryptophan. J. Biol. Chem. 235: 1898-1102. 22. Murashige, T. and F. Skoog. 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497. 23. Ranch, P. J., S. Rick, J. E. Brotherton and T. M. Widholm. 1983 Expression of 5-methyltryptophan resistance in plants regenerated from resistant cell lines of Datura innoxia. Plant Physiol. 71:136-148. 24. Sung, Z. R. 1979 Relation of indole-3-acetic acid and tryptophan concentrations in normal and 5-methyltryptophan-resistant cell lines of wild carrots. Planta 145:339-345. 25. Wakasa, K. and J. M. Widholm. 1987 A 5-methyltryptophan resistant rice mutant, MTR1, selected in tissue culture. Theor. Appl. Genet. 74:49-54. 26. Widholm, J. M. 1971 Control of tryptophan biosynthesis in plant tissue cultures: lack of repression of anthranilate and tryptophan synthases by tryptophan. Physiol. Plant. 25:75-79. 27. Widholm, J. M. 1972a Tryptophan biosynthesis in Nicotiana tabacum and Daucus carota cell cultures: site of action of inhibitory tryptophan analogs. Biochim. Biophys. Acta 261:44-51. 28. Widholm, J. M. 1972b Cultured Nicotiana tabacum cells with an altered anthranilate synthase which is less sensitive to feedback inhibition. Biochim. Biophys. Acta 261:52-58. 29. Widholm, J. M. 1972c Anthranilate synthase from 5-methyltryptophan-susceptible and -resistant cultured Daucus carota cells. Biochim. Biophys. Acta 279:48-57. 30. Widholm, J. M. 1974a Cultured carrot cell mutants: 5-methyltryptophan-resistance trait carried from cell to plant and back. Plant Sci. Lett. 3:323-330. 31. Widholm, J. M. 1974b Evidence for compartmentation of tryptophan in cultured plant tissues: free tryptophan levels and inhibition of anthranilate synthase. Physiol. Plant. 30:323-326. 32. Widholm, J. M. 1977a Relation between auxin autotropy and tryptophan accumulation in cultured plant cells. Planta 134:103-108. 33. Widholm, J. 1977b Selection and characterization of amino acid analog resistant plant cell cultures. Crop Sci. 17:597-600. 34. Widholm, J. M. 1978 Selection and characterization of a Daucus carota L. cell line resistant to four amino acid analogues. J. Exp. Bot. 29:1111-1116. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75649 | - |
| dc.description.abstract | 利用單倍體Nicotiana plumbaginifolia Viviani (2n＝20)的葉肉原生質體，碟養在含有抑制生長濃度的5-甲基色氨酸(5-methyltryptophan)培養基，共篩選出8個抗性品系。抗性品系再生植株維持在蔗糖濃度為3％的MS培養基，會有頂芽優勢丟失，葉片發育受阻，莖切口處形成癒合組織及不長根等現象。抗性品系與野生型癒合組織內芳香族氨基酸的含量並無顯著差異。以14C標定的色氨酸(tryptophan)做吸收試驗，發現抗性品系的葉片對色氨酸的吸收量比野生型顯著降低（約為野生型的50?64％）。這些結果顯示抗性品系對5－甲基色氨酸的抗性可能與5－甲基色氨酸的吸收能力降低有關。 | zh_TW |
| dc.description.abstract | Eight 5-methyltryptophan-resistant lines were isolated from Micotiana plumbaginifolia Vivinia (2n=20) upon plating mesophyll protoplasts of haploid plants in agarose medium containing a growth inhibitory concentration of the analogue. The resistant plants lost apical dominance, showed impeded leaf development, formed callus at the cut end of the stem, and could not form root in MS medium containing 3% sucrose. There were no significant differences in the content of aromatic amino acids between resistant and wild-type callusse. In a (14C) tryptophan uptake experiment, leaf slices of resistant plants uptook significantly reduced amount of tryptophan than those of wild-type (about 50 ? 64% of wild-type). These results showed that the resistance of the variants was related to a reduced uptake of 5-methyltryptophan. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:25Z (GMT). No. of bitstreams: 0 Previous issue date: 1988 | en |
| dc.description.tableofcontents | Ⅰ．中文摘要……………………………………………………1 Ⅱ．英文摘要……………………………………………………2 Ⅲ．緒 言……………………………………………………3 Ⅳ．材料與方法…………………………………………………11 Ⅴ．結 果……………………………………………………20 Ⅵ．討 論……………………………………………………35 Ⅶ．參考文獻……………………………………………………41 | |
| dc.language.iso | zh-TW | |
| dc.title | Nicotiana plumbaginifolia抗5－甲基色氨酸變異體之篩選及定性 | zh_TW |
| dc.title | Selection and characterization of 5-methyltryptophan-resistant variants in Nicotiana plumbaginifolia | en |
| dc.date.schoolyear | 76-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 47 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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