請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75622完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陸知慧 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:15Z | - |
| dc.date.available | 2021-07-01T08:14:15Z | - |
| dc.date.issued | 1986 | |
| dc.identifier.citation | 參考文獻 Beyer, K. F. and Samuel, L. T. (1956). Distribution of steroids-3β-ol-dehydrogenase in cellular structure of the adrenal gland. J. Biol. Chem. 219: 69-76. Bulter, D.G. (1973) Structure and function of the adrenal gland of fishes. American Zoologist 13(3): 839-679. Chester Jones, I. Chan, K. D. D., Handerson, I. W. and Ball, J. N. (1969). The Adrenocortical steroids, adrenocorticotropin and the corpuscles of Stannius. In W. S. Hoar and D. J. Randal (eds.) “Fish Physiology”, Academic press New York, 2 321-376. Colombo, L.., LLLBern, H.A., Pieprzyk, J. and Johnson, D.W. (1972) Corticosteroidogenesis in; vitro by the head kidney of Tilapia Mossambica (Cichlidae, Teleostei). Endocrin; ology 91:450-462. Ferguson, Jr. J. J. (1972). The mechanism of action of adrenocorticotropic hormone. In Litwack, C. (ed.) “Biochemical Actions of Hormones” Academic press New York, London. 2: 317-335. Goldberg, B., Jones, C. E. S. and Borkowf, H. I. (1964). A histochemical study of substrate specificity for the steroid-3-ol-dehydrogenase and isomerase systems in human ovary and testis. J. Histochem. Cytochem. 12: 880-869. Gwynne, J.T., Manaffee, D., Brewer, J.R.H.B. and Ney R.L. (1976) Adrenal cholesterol uptake from plasma lipoprotein: regulation by corticotropin. Proc. Natl. Acad. Sci. U.S.A. 73:4329-4336. Hiwatoshi, A. and Ichikawa, Y. (1981). Purefication and reconstitution of the steroid 21-hydroxylase system (cytochrome p-450-linked mixed function oxidase system) of bovine adrenocortical microsome. Biochem. Biophys. Acta. 664: 33-48. Jaffe, R. B., Seron-ferre, M., Crickard, K., Koritnik, D., Mitchell, B. F. and Huhtaniemi, I. T. (1981). Regulation and function of the primate fetal adrenal gland and gonad. Recent Prog. Horm. Res. 37: 41-104. Ilan, Z. and Yaron, Z. (1976) Stimulation of carp interrenal function by adrenocorticotropin. J. Endocrinol. 68:269-277 Kawauchi, H., Abe, K.I. and Tadahashi, A. (1980) Corticotropin like intermediate lobe peptide in the salmon pituitary. Bull. Jap. Soc. Sci. Fish. 46:743-747 Li, C.H., Dixon, J.S. and Chung, D. (1961) Adrenocorticotropins XXI. The amino acid sequence of bovine adrenocorticotropin. Biochim. Biophys. Acta. 46:324-334 Lowry, P.J.., Hugh, P., Bennett, J. and McMartin, C. (1974) The isolation and amino acid sequence of an adrenocorticotropin from the pars distalis and a corticotropin-like-intermediate-lobe-peptide from the neurointermediate lobe of the pituitary of the Dogfish Shi Biochem. .J. 141: 47-437. Nakano, H., Takemoto, C., Sato, H. and Tamaoki, B. I. (1968). Location of hydroxy groups introduced to steroid molecules by adrenal and testicular enzyme I. Biochem. Biophys. Acta. 152: 186-196. Nandi, J. and Bern, H.A. (1959) In vitro production of corticosteroids by the interrenal tissue of teleost fishes. Anat. Rec. 134:614-615. Nandi. J. and Bern, ILA. (1960) Corticosteroid production by the interrenal tissue of teleost fishes. Endocrinology 66:295-303 Phillips, J.G. and Bellamy, D. (1963) Adrenocortical Hormones. In Euler, V. and Heller, H. (eds.)“Comparative Endocrinology” Academic press, New York, 1: 208-257. Pickford, G. E. and Atz, .J. W. (1957). “The Physiology of the Pituitary Gland of Fishes”. New York, Zoological Scociety. Ramachandra, J. (1975). The structure and function of adrenocorticotropin. In Li, C. H. (ed.)”Hormonal proteins and peptides”. Academic press, New York, San Francisco, London. 2: 1-28. Rance, T. A. and Baker, B. I. (1981). The in vitro response of the trout .interrenal to various fragments of ACTH. Gen. Comp. Endocrinol. 45: 497-503. Sayers, G., Swallow, R. L. and Giordano, N. B. (1971). An improved technique for the preparation of isolated rat adrenal cells, A sensitive, accuate and specific method for the assay of ACTH. Endocrinology 88: 1063-1068. Sandor, T. and Mrndi, A. Z. (1979). Steroids and evolution. In Barrington, E. J. W. (ed.) “Hormones and Evolution” Academic press, London, New York, San Francisco. 1: 1-72. Williams, R. H. (1981). The adrenals. “Textbook of Endocrinology” (6th edition) pp. 249-290. Igaku-Shoin/Saunders International Edition. 柯逢春(1983) 鯉魚頭腎皮質固酮之研究(碩士論文) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75622 | - |
| dc.description.abstract | 摘要 本實驗的目的在於分離草魚腦下腺腎上腺皮質刺激素（Adrenocorticotropin, ACTH)。 草魚腦下腺以0.1N醋酸萃取，將上層液冷凍乾燥，利用HPLC做初次分離，分別以階梯式滌流，分別收取10％、40％、60％、80％之氰甲烷（含0.07％ TFA)滌流物，其中10?40％氰甲烷（含0.07％ TFA）滌流物具最強之皮質刺激素活性。將此部分冷凍乾燥後，再以HPLC做第二次分離。 第二次分離時，以10?40％氰甲烷（含0.07％ TFA）展開，滌流1.5小時，使最終氰甲烷濃度達於40％。每5分鐘收取一分液，冷凍乾燥後做生物活性測定，其中以F7、F8、F9、F10之活性最強，經劑量效應，以4倍及16倍稀釋，再測生物活性以及測F7?F10之間各吸收?之活性，發現以F8、F9具最強的皮質刺激素活性。分別收集此二部分，匯在一起，冷凍乾燥後以HPLC做第三次分離。 第三次分離時，同樣以第二次HPLC解析條件解析之。將各吸收?回收做生物活性測定，結果得一活性極強且單一之吸收?的物質。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:15Z (GMT). No. of bitstreams: 0 Previous issue date: 1986 | en |
| dc.description.tableofcontents | 目錄 壹、摘要.....................l 貳、緒言.....................3 參、材料與方法.....................7 一、化學藥品.....................7 二、儀器裝置.....................7 三、草魚腦下腺來源.....................8 四、草魚腦下腺醋酸萃取操作.....................8 五、腦下腺醋酸萃取物的HPLC解析.....................9 六、頭腎組織之製備.....................9 七、離體組織培育.....................9 八、固酮類之萃取.....................10 九、HPLC解析固酮類及定量.....................10 肆、結果.....................12 一、鯉魚頭腎之反應.....................12 二、初次純化.....................12 三、二次純化.....................17 四、三次純化.....................17 伍、討論.....................30 陸、參考文獻.....................33 | |
| dc.language.iso | zh-TW | |
| dc.title | 草魚腦下腺腎上腺皮質刺激素之初步純化 | zh_TW |
| dc.date.schoolyear | 75-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 37 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。