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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75617完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Heng lee | en |
| dc.contributor.author | 李珩 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:13Z | - |
| dc.date.available | 2021-07-01T08:14:13Z | - |
| dc.date.issued | 1987 | |
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Abate,C.,Luk,D.,Gentz,R.,Rauscher III and F. J., Curran,T. (1990). Expression and purification of the leucine zipper,and DNA-binding domain of Fos and Jun: both Fos and Jun contact DNA directly. Proc. Natl. Acad. Sci. USA.87,1032-1036. 7. Abel,T. and Maniatis,T. (1989). Action of leucine zippers. Nature 341,24-25. 8. Ryder,K.,Lau,L. F. and Nathans,D. (1988). A gene activated by growth factors is related to the oncogene v-jun. Proc. Natl. Acad. Sci USA. 85,1487-1491. 9. Ryder,K.,Lanahan,A.,Perez-Albuerne,E. and Nathans,D. (1989). Jun-D a third member of the Jun gene family. Proc. Natl. Acad. Sci USA. 86,1500-1503. 10. Lamph,W. W.,Wamsley,P.,Sassone-Corsi,P. and Verma,I. M. (1988). Induction of proto-oncogene junAP-1 by serum and TPA. Nature 334,629-631. 11. Ryseck,R. -P.,Hirai,S. I.,Yaniv,M. and Bravo,R. (1988). Transcriptional activation of c-jun during the G0/ G1 transition in mouse fibroblast. Nature 334,535-537. 12. Quantin,B. and Breathnach,R. (1988). Epidermal growth factor stimulates transcription of the c-jun protooncogene in rat fibroblast. Nature 334,538-539. 13. Ryder,K. and Nathans,D. (1988). Induction of protooncogene c-jun by serum growth factors. Proc. Natl Acad. Sci. 85,8464-8467. 14. Wu,Bei-yue,Fodor,Eric J. B.,Edwards,R. H. and Rutter,W. J. (1989). Nerve growth factor induces the protooncogene c-jun in PC12 cells. J. Biol. Chem. 264,9000-9003. 15. Brenner,D. A.,O?Hara,M.,Angel,P.,Chojkier,M. and Karin,M. (1989). Prolonged activation of jun and collagenase genes by tumour necrosis factor alpha. Nature 337,661-663. 16. Rittling,S. R.,Coutinho,L.,Amram,T. and Kolbe,M.(1989). AP-1/jun binding sites mediate serum inducibility of the human vimentin promoter. Nucl. Acids Res. 17,1619-1632. 17. Sonnenberg,J. L.,Rauscher III,F. J.,Morgan,J. I. and Curran,T. (1990). Regulation of proenkephalin by fos and jun. Science 246,1622-1625. 18. Weiner,F. R.,Czaja,M. J.,Jefferson,D. M.,Giambrone,M-A.,Tur-Kaspa,R.,Reid,L. M. and Zern,M. A. (1987). The effects of dexamethasone on in vitro collagen gene expression. J. Biol. Chem. 262,6955-6958. 19. Frisch,S. M.,Ruley,H. E.,(1987). Transcription from the stromelysin promoter is induced by interleukin-1 and repression by dexamethasone. J. Biol. Chem. 262,16300-16304. 20.Drouin,J.,Charron,J.,Gagner,J.P.,Jeannotte,L.,Nemer,M.,Plante,R. K. and Wrange,O. (1987). The proopiomelanocortin gene: a model for negative regulation of transcription by glucocorticoids. J. Cell. Biochem. 35,293-304. 21. Sakai,D. D.,Helms,S.,Carlstedt-Duke,J.,Gustafsson,J. A.,Rottman,F. M. and Yamamoto,K. R. (1988). Hormone-mediated repression of transcription: a negative glucocorticoid response element from the bovine prolactin gene. Genes Dev.2,1144-1154. 22. Akerblom,I. E.,Slater,E. P.,Beato,M.,Baxter,J. D. and Mellon,P. L. (1988). Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer. Science 241,350-353. 23. Goustin,A. S.,Leof,E. B.,Shipley,G. D. and Moses,H.L. (1986). Growth factors and cancer. Cancer Res. 46,1015- 1029. 24. Sachs,L. (1986). Growth,differentiation and the reversal of malignancy. Sci. Ame. 254(1),30-37. 25. J. Cell. Biochem.33,213-218. 26. Ausubel,F. M.,Brent,R.,Kingston,R. E.,Moore,D. D.,Seidman,J. G.,Smith,J. A. and Struhl,K. (1987). Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley-lnterscience,ed. 27. Chomczynski,P. and Sacchi,N. (1987). Single-step method of RNA isolation by acid quanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochem. 162,156-159. 28. Gorman,C. (1985). in DNA cloning vol. II --- a practical aproach. Glover,D. M.,ed.,155-158. 29. Rosenthal,N. (1987). in Method in Enzymology vol. 152, Berger,S. L. and Kimmel,A. R.,ed.,Academic Press,717-719. 30.. Ochman,H.,Ajioka,J. W.,Garza,D. and Harti,D. L. (1989). in PCR Technology --- principle and application for amplification,Erlich,H. A.,ed.,Stockton Press. 31. Hattori,K.,Angel,P.,Le Beau,M. M. and Karin,M.(1988). Structure and chromosomal locolization of the functional intronless human jun proto-oncogene. Proc. Natl. Acad. Sci USA. 85,9148-9125. 32. Angel,P.,Hattori,K.,Smeal,T. and Karin,M. (1988). The jun proto-oncogene is positively autoregulated by its product,jun,AP-1. Cell 55,875-885. 33. Ham,J. and Parker,M. G. (1989). Regulation of gene expression by nuclear hormone receptors. Current Opinion in Cell biology 1,503-511. 34. Beato,M.,(1989). Gene regulation by steroid hormones. Cell 56,335-344. 35. Levine,M. and Manley,J. L. (1989). Transcriptional repression of eukaryotic promoters. Cell 59,405-408. 36. Mordacq,J. C. and Linzer,I. H. (1989). Co-localization of elements required for phorbol ester stimulation and glucocorticoid repression of proliferin gene expression. Genes Dev. 3,760-769. 37. Collins,F. S. and Weissman,S. M. (1984). Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method. Proc. Natl. Acad. Sci.81,6812-6816. (for review) 38. Marx,J. L. (1988). jun is bustin’ out all over. Science 242,1377-1378. 39. Curran,T. and Franza,B. R. (1988). Fos and jun: the AP-1 connection. Cell55,395-397. 40.Ball,Jr.,A.R.,Bos,T.J.,Loliger,C.,Nagata,L.P.,Nishimura,T,Su,H.,Tsuchie,H. and Vogt,P. K. (1988).jun oncogene and transcriptional regulator,Cold Spring Habor Symposia on Quantitative Biology vol LIII,687-693. 41. Ransone,L. J. and Verma,I. M. (1989). Association of nuclear oncoproteins fos and jun Current Opinion in Cell Biology 1,536-540. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75617 | - |
| dc.description.abstract | 致癌基因jun在1987年被發現於禽類肉瘤病毒 (avian sarcoma virus 17)．目前已知其產物可與另一致癌基因fos之產物形成異質雙體（heterodimer),結合於AP-1位置上，遂行轉錄活化的作用．由於c-jun 的基因表現受到許多生長因數的影響（例如表皮生長因數，神經生長因數等等），我們預期其亦可接受一些荷爾蒙的調控，結果發現在加入類皮質糖荷爾蒙後4-12小時間產生約2倍的抑制效果，且此種抑制並不受到轉譯抑制劑的影響，為一原級反應．此種抑制的產生可能由於類皮質糖荷爾蒙受體與AP-1相互競爭結合位的結果，經由CAT檢定，發現該受體在AP-1位置附近有一弱結合位，此結果初步證明瞭這個假設． | zh_TW |
| dc.description.abstract | The Jun oncogene has been identified in avian sarcoma virus 17 in 1987. The products of jun and fos form a heterodimer which can bind to AP-1 site and function as a transcriptional activator. Expression of c-jun proto-oncogene is generally regulated by various growth factors (e.g. epidermal growth factor, nerve growth factor). The first result of our experiments shows that after the treatment of glucocorticoid hormone, a twofold repression of transcriptional activity of c-jun can be observed within a 4-12 hours period. Further experiments prove such repression as a primarily response since it is not influenced by the presence of cycloheximide--a translation inhibitor. The repression may be due to a competitive binding between glucocorticoid receptor and Jun—Fos complex. This assumption is primarily proved by CAT assay which indicates a weak binding of this hormone receptor surrouding AP-1 site. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:13Z (GMT). No. of bitstreams: 0 Previous issue date: 1987 | en |
| dc.description.tableofcontents | 壹．中文摘要........................第 1 頁 貳．英文摘要........................第 2 頁 參．緒言............................第 3 頁 肆．實驗步驟........................第 5 頁 伍．試劑............................第 10 頁 陸．結果............................第 13 頁 柒．討論............................第 24 頁 捌．英文縮寫表......................第 30 頁 玖．參考資料........................第 31 頁 謝辭 ...............................第 35 頁 | |
| dc.language.iso | zh-TW | |
| dc.title | 類皮質糖荷爾蒙對 c-jun 基因表現之影響 | zh_TW |
| dc.title | The Effects of Glucocorticoid Hormone on the Expression of c-jun in NIH 3T3 Cells | en |
| dc.date.schoolyear | 75-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 38 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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