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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Ken,Ruey | en |
| dc.contributor.author | 耿瑞 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:05Z | - |
| dc.date.available | 2021-07-01T08:14:05Z | - |
| dc.date.issued | 1985 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75595 | - |
| dc.description.abstract | 摘要 因B型肝炎病毒（HBV）感染而引起的病毒性肝炎在台灣是一個嚴重的公共衛生問題。以此病毒的表面抗原（HBsAg）製成疫苗，以核心抗原（HBcAg）製成檢定試劑，皆為解決此病毒為害人類的醫療策略。 本實驗乃是嘗試利用基因表現載體（expression vector): pLG669Z, pHC624-iqI-tac，及基因重組（recombinant DNA）技術，分別在酵母系統及大腸桿菌系統中，選殖表面抗原基因及核心抗原基因，並測其表現，以為生產此二抗原之先導實驗。 | zh_TW |
| dc.description.abstract | Summary Hepatitis B virus (HBV) infection is a serious public health problem in Taiwan. Hepatitis B surface antigen (HBsAg) has been successfully used as vaccine for preventing hepatitis B. Both HBsAg and HBcAg are useful reagents for the screening and diagnosis of HBV infection. The current state of art of obtaining sufficient amounts of hepatitis B surface antigen and hepatitis B core antigen is cloning and expressing the corresponding genes in suitable vectors. In this research, HBsAg gene-containing DNA fragment prepared by various restriction endonuclease digestion and/or Bal 31 exonuclease treatment has been cloned into E. coli and yeast shuttle vector pLG669Z. HBcAg gene-containing DNA fragment prepared by the similar methods has been cloned into E. coli vector pHC624-iqI-tac. The expression of both HBsAg and HBcAg is going to be analyzed elsewhere. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:05Z (GMT). No. of bitstreams: 0 Previous issue date: 1985 | en |
| dc.description.tableofcontents | 目錄 英文摘要. . . . . . . . . . . . . . . .l 中文摘要. . . . . . . . . . . .. . . .2 緒言. . . . . . . . . . . . . .3 試藥. . . . . . . . . . .. . .11 方法 大量抽取大腸桿菌中Plasmid DNA . . . . . . . . . . .. . . 18 少量大腸菌DNA快速鹼解法. . . . . . . . . . .. . . 19 限制?反應以分離DNA片段. . . . . . . . . . .. . . 20 限制?作用以篩選菌株. . . . . . . . . . .. . . 21 Bal 31 exonulease切割. . . . . . . . . . .. . . 22 DNA Polymerase I large fragment反應. . . . . . . . . . .. . . 22 Blunt end DNA片段與linker之連接及切成cohesive end . . . . . . 22 載體之處理. . . . . . . . . . .. . . 23 Cohesive end DNA片段與載體之連接. . . . . . . . . . .. . . 24 大腸桿菌competent cell之製備. . . . . . . . . . .. . . 24 大腸桿菌之transformation . . . . . . . . . . .. . . 25 酵母菌的transformation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 少量快速抽取酵母菌DNA法. . . . . . . . . . . . . . . 27 Nick-translation反應. . . . . . . . . . . . . . .27 Southern blot . . . . . . . . . . . . . . 28 測HBc Ag 之表現. . . . . . . . . . . . . . 29 測HBs Ag之表現. . . . . . . . . . . . . . 30 結果. . . . . . . . . . . . . .31 討論. . . . . . . . . . . . . . 53 參考資料. . . . . . . . . . . . . . 59 | |
| dc.language.iso | zh-TW | |
| dc.title | B型肝炎病毒表面抗原及核心抗原基因之選殖 | zh_TW |
| dc.title | Cloning of Hepatitis B Surface Antigen and Core Antigen Genes | en |
| dc.date.schoolyear | 74-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 67 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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