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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Jun-Hsiang Lin | en |
| dc.contributor.author | 林潤庠 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:51Z | - |
| dc.date.available | 2021-07-01T08:13:51Z | - |
| dc.date.issued | 1986 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75558 | - |
| dc.description.abstract | 本實驗是利用一種從真泥鰍(Misqurnus anquillicandatus)所培養出來的泥鰍鰭細胞株(loach fin cell line; LF cell)，全盤性探討紫外線對泥鰍細胞的影響，並和哺乳類細胞作比較。本實驗概括地可分為四部分：(1)紫外線對細胞存活率的影響。(2)紫外線對於細胞DNA複製的幹擾。(3)紫外線造成細胞DNA上傷害的修補一一包括切除式修補(excision repair)和光活化修補(photoreactivation repair)。(4)DNA合成抑制劑對切除式修補方法的影響。 結果發現泥鰍細胞對於紫外線相當敏感，進一步推測其原因與下列現象有密切關係：(一)經紫外線照射後，泥鰍細胞的DNA複製遭受相當大的幹擾，這表現在DNA合成速率的急速下降和新合成DNA分子(nascent DNA)的大小分佈的明顯變化上，需要長時間的培養後，才能慢慢恢復正常。(二)對於紫外線在DNA上造成的傷害，泥鰍細胞的切除式修補能力很差，無法用一般傳統方法偵測到，需要改用靈敏度相當好的擬核沉降法(Nucleoid sedi-mentation)才能測出。至於泥鰍細胞的複製後修補(post-replication repair)能力與哺乳類細胞近似。 泥鰍細胞的光活化修補能力非常旺盛，細胞經過紫外線照射後，再予以可見光處理，則細胞存活率顯著提升，而DNA合成速率的被抑制程度則明顯減小。最後，發現泥鰍細胞的DNA合成和切除式修補對於某些DNA合成抑制劑，具有相當的抵抗性(resistance)。 | zh_TW |
| dc.description.abstract | Cell line derived from loach (Misgurnus anguillicandatus) fins has been shown to be hypersensivity to ultraviolet light (UV) radiation Compare with normal human skin fibroblast cells the colony formation ability, the rate of DNA synthesis ,the inhibition mode of nascent DNA distribution and recorvery of normal-sized nascent DNA replication intermediates all showed great retardation after UV damage. However the rate of forming high molecular weight-replicative intermediates after initiation (postreplicaton repair) is not affected. UV-induced excision repair could hardly be shown by coventional means; only through one of the most sensitive methods--nucleoid sedimentation, which measures the relaxation of DNA supercoils in chromatin--could the single strand breakage be detected. The defective incision ability may render th loach cells is sensitive to UV and other UV-like damages. Both replicative DNA synthesis and repair DNA synthesis of loach cells are resistant to inhibitors of DNA synthesis-- hydroxyure (HU) and arabinofuranosylcytosine (araC), but another specific inhibitor--aphidicolin exerts the drastic inhibition effect. Loach cells have efficient photoreactivation repair ability. Subsequent expoure to visible light would enhance the cell survival and rate of DNA synthesis after UV irradiation. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:51Z (GMT). No. of bitstreams: 0 Previous issue date: 1986 | en |
| dc.description.tableofcontents | 一、中文、英文摘要………I, II, III 二、謝辭………………………IV 三、內容 壹、緒言…………………………1 貳、材料與方法………………11 2-1 細胞株與細胞培養……………11 2-2 紫外線處理後的細胞存活率………12 2-3 紫外線照射後DNA合成速率的變化……13 2-4 紫外線照射後新合成DNA分子大小分佈的變化………15 2-5 複製後修補能力的測定……………………………17 2-6 切除式修補能力的測定……………………………17 2-6-1 鹼性蔗糖梯度中DNA解鏈速率測定法………17 2-6-2 中性蔗糖梯度擬核沉降法…………………………18 2-7 DNA合成抑制劑對DNA合成總量的影響…………21 2-8 DNA合成抑制劑對切除式修補的影響……………22 2-9 光活化修補能力的測定………………………………22 2-9-1 光活化作用對紫外線照射後細胞存活率的影響………23 2-9-2 光活化作用對紫外線照射後DNA合成速率的影響………23 三、結果………24 肆、討論………………38 四、圖表……………51 五、參考文獻………70 | |
| dc.language.iso | zh-TW | |
| dc.title | 探討對紫外線高度敏感的泥鰍鰭細胞 | zh_TW |
| dc.title | Hypersensitiviy of A Loach Fin Cell Line | en |
| dc.date.schoolyear | 74-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 74 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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