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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 王聖予 | zh_TW |
dc.date.accessioned | 2021-07-01T08:13:46Z | - |
dc.date.available | 2021-07-01T08:13:46Z | - |
dc.date.issued | 1985 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75542 | - |
dc.description.abstract | 本實驗的目的,在於建立大肚魚(Top-minnow)之細胞株,並對此細胞株進行一些生物學特性之研究。 此細胞株在含10%牛犢血清的Leibovitz's L-15培養液中,於31℃的條件之下已繼代培養了84次,並命名為TM。細胞之形態呈纖維母細胞狀,表面附著效率值約為11.7%,分析48代及60代分裂中期細胞的染色體數目,顯示其分佈在21到68之間,而以含38個染色體的細胞最多。TM細胞之生長在一定的限度之內會隨著血清濃度的增加而上昇,在完全不含血清的培養液中,生長停頓,細胞甚至不會附著在培養瓶上。TM細胞經過一段時間的培養後,細胞會形成單層細胞層(monolayer),而不會有重疊或堆積成塊的現象,表示此細胞具有接觸性抑制作用(contact inhibition),證明此細胞並未變性(Transformation)。掃描式電子顯微鏡下觀察TM細胞的表面微細形態,顯示在正常狀況下的細胞在表面會生出許多的微絨毛(microvilli),這些微絨毛長度很長,當環境欠佳或細胞老化時,微絨毛會漸漸變短,進而被一些乳狀突起(bleb)所取代,而後此乳狀突起亦會消失,細胞會有凹陷、穿孔的現象,最後細胞發生自體溶解(autolysis)。 實驗顯示TM細胞對於魚類病毒EVE、EVEX、EVA、IPNV及LV-1均具有感受性,而且複製量很高,在經過液態氮長期的保存下,仍具有很好的生存能力,TM細胞經過鑑定沒有細菌、?菌及黴漿菌(mycoplasma)的感染。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:46Z (GMT). No. of bitstreams: 0 Previous issue date: 1985 | en |
dc.description.tableofcontents | 一、摘 要………………………………………………………………………………………………I 二、謝 辭………………………………………………………………………………………………III 三、目 錄………………………………………………………………………………………………IV 四、內 容 第一章 緒 論………………………………………………………………………………………1 第二章 材料與方法…………………………………………………………………………………3 第一節 材 料 2.1-1 大肚魚…………………………………………………………………………………3 2.1-2 磷酸?緩衝溶液………………………………………………………………………3 2.1-3 組織洗滌液……………………………………………………………………………4 2.1-4 胰蛋白?溶液…………………………………………………………………………4 2.1-5 培養液…………………………………………………………………………………4 2.1-6 Colcemid………………………………………………………………………………5 2.1-7 Carnoy's固定液………………………………………………………………………5 2.1-8 低張溶液………………………………………………………………………………5 2.1-9 Giemsa染料溶液………………………………………………………………………6 2.1-10 電子顯微鏡標本製備試劑……………………………………………………………6 2.1-11 TSA 培養基……………………………………………………………………………8 2.1-12 PDA 培養基……………………………………………………………………………8 2.1-13 Mycoplasma stain kit………………………………………………………………8 2.1-14 Mounting medium ……………………………………………………………………9 第二節 方 法 2.2-1 大肚魚細胞之初期培養………………………………………………………………9 2.2-2 細胞繼代培養…………………………………………………………………………10 2.2-3 染色體數目之分析……………………………………………………………………10 2.2-4 牛犢血清濃度對細胞生長速率之影響………………………………………………11 2.2-5 溫度對細胞生長速率之影響…………………………………………………………12 2.2-6 不同培養液對細胞生長速率之影響…………………………………………………12 2.2-7 掃描電子顯微鏡之觀察………………………………………………………………12 2.2-8 細胞之冷凍貯存………………………………………………………………………13 2.2-9 細胞之表面附著效應…………………………………………………………………14 2.2-10 細胞對於病毒之感受性………………………………………………………………14 2.2-11 TCID50…………………………………………………………………………………15 2.2-12 細胞污染檢驗…………………………………………………………………………16 第三章 結 果 3.1 大肚魚細胞之初期培養………………………………………………………………………18 3.2 細胞繼代培養…………………………………………………………………………………18 3.3 染色體數目之分析……………………………………………………………………………19 3.4 牛犢血清濃度對細胞生長速率之影響………………………………………………………19 3.5 溫度對細胞生長速率之影響…………………………………………………………………20 3.6 不同培養液對細胞生長速率之影響…………………………………………………………20 3.7 掃描電子顯微鏡之觀察………………………………………………………………………20 3.8 細胞的冷凍貯存………………………………………………………………………………21 3.9 細胞之表面附著效應…………………………………………………………………………21 3.10 細胞對於病毒之感受性………………………………………………………………………22 3.11 細胞污染鑑定…………………………………………………………………………………22 第四章 討 論…………………………………………………………………………………………23 圖 表………………………………………………………………………………………………………28 參考文獻……………………………………………………………………………………………………41 | |
dc.language.iso | zh-TW | |
dc.title | 大肚魚細胞株之建立及其生物學特性之研究 | zh_TW |
dc.title | Studies on the Biological Characteristics of A Cell Line Derived from Top-minnow (Gambusia Patruelis) | en |
dc.date.schoolyear | 73-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 45 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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