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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75541完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Hsi-Chieh Wang | en |
| dc.contributor.author | 王錫傑 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:46Z | - |
| dc.date.available | 2021-07-01T08:13:46Z | - |
| dc.date.issued | 1985 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75541 | - |
| dc.description.abstract | 近年來環境污染日趨嚴重，為測量河川污染程度，選用魚類的存活率是一個很好的指標，然而不同的魚對於污染物有著不同程度的忍受力。本實驗乃是以本省農地使用量甚多的三種除草劑：丁基拉草（Butachlor）、巴拉刈（Paraquat）、殺丹（Saturn），及一種殺蟲劑：亞素靈（Azodrin），探討及比較對三種魚類細胞株：泥鰍鰭細胞株（Loach fin cell line, LF）、吳郭魚腎臟細胞株（Tilapia kidney cell line, TK）及大肚魚細胞株（Top-minnow cell line, TM）的毒性反應，並藉以對細胞的生理情況有進一步的瞭解。 1．在細胞毒性反應中，三種除草劑皆有毒性，且隨農葯之濃度昇高，細胞的存活率降低。殺蟲劑Azodrin在800μM內，不具明顯的毒性效應。 2．四種農葯對三種魚類細胞株的毒性比較皆為Butachlor的毒性大於Paraquat大於Saturn大於Azodrin。 3．細胞對農葯的抵抗性（resistance），Butachlor為TK＞LF＞TM，Paraquat為TK＞TM＞LF，Saturn為TM＞LF＞TK。 4．對LF細胞DNA合成速率之影響，Butachlor、Paraquat及Saturn皆有50％?70％之抑制效果，當葯物移除則可完全回復，而對TK細胞亦有50％?80％之抑制，但為不可逆。TM細胞全部農葯皆有40％?60％之抑制效果，亦可回復。 5．四種農葯對TM細胞之RNA合成速率皆有影響，三種除草劑對TK細胞亦有20％?60％的抑制作用，對LF細胞只有Saturn有20％?30％的抑制效果。 6．Butachlor及Saturn對三種細胞株之蛋白質合成速率有不同程度的影響。LF細胞最大（60％?80％），TK細胞次之（30％?40％），TM細胞最小（10％?30％），Paraquat在TM細胞略有抑制（20％）。 7．從Superoxide dismutase （SOD）活性之測定顯示LF細胞對Paraquat最為敏感，可能與其SOD活性在高濃度Paraquat處理後降低有關。 8．Paraquat 10μM及50μM處理LF細胞一小時，對LF細胞細胞週期沒有明顯的影響。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:46Z (GMT). No. of bitstreams: 0 Previous issue date: 1985 | en |
| dc.description.tableofcontents | 一、學位論文審查合格證明書………………………………………………………………………i 二、摘要………………………………………………………………………………………………ii 三、謝辭………………………………………………………………………………………………iv 四、目錄………………………………………………………………………………………………v 五、內容 壹、緒言……………………………………………………………………………………………1 貳、材料與方法 一、細胞株與細胞培養………………………………………………………………………7 二、農葯來源…………………………………………………………………………………8 三、農葯對細胞的毒性反應…………………………………………………………………9 四、農葯對細胞內大分子合成速率的影響…………………………………………………10 a、DNA合成速率…………………………………………………………………………10 b、RNA合成速率…………………………………………………………………………11 c、蛋白質合成速率………………………………………………………………………12 五、Superoxide dismutase酵素活性檢定…………………………………………………12 a、蛋白質濃度之測定 …………………………………………………………………13 b、Superoxide dismutase 檢定………………………………………………………13 六、巴拉刈對泥鰍細胞細胞週期的影響……………………………………………………14 三、結果 一、農葯處理對細胞存活的影響……………………………………………………………17 二、農葯處理對細胞DNA合成速率的影響 …………………………………………………19 三、農葯處理對細胞RNA合成速率的影響 …………………………………………………20 四、農葯處理對細胞蛋白質合成速率的影響………………………………………………21 五、巴拉刈處理後對細胞內SOD酵素活性的影響 …………………………………………22 六、巴拉刈對泥鰍細胞之細胞週期的影響…………………………………………………23 肆、討論 一、農葯處理對細胞存活的影響……………………………………………………………24 二、農葯處理對細胞DNA合成速率的影響 …………………………………………………26 三、農葯處理對細胞內RNA及蛋白質合成速率的影響 ……………………………………28 四、巴拉刈處理後對細胞內SOD酵素活性的影響 …………………………………………31 五、巴拉刈對泥鰍細胞之細胞週期的影響…………………………………………………33 六、圖表………………………………………………………………………………………………38 七、參考文獻…………………………………………………………………………………………71 | |
| dc.language.iso | zh-TW | |
| dc.title | 比較四種農藥對三種魚類細胞株的毒性反應 | zh_TW |
| dc.title | Toxic Effect of Four Pesticides on Three Fish Cell Lines | en |
| dc.date.schoolyear | 73-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 83 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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