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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陶建英 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:33Z | - |
| dc.date.available | 2021-07-01T08:13:33Z | - |
| dc.date.issued | 1984 | |
| dc.identifier.citation | 1．中澤亮治、武田養人、中野政弘1937．日本農藝化學會誌，13,52-62 2．陳晚進1972.利用澱粉粕生產檸檬酸之研究，國立台尚大學農業化學研究所碩士論文。3．章麗雲1982．利用米酒廠廢液進行檸檬酸醱酵，國立臺灣大學植物科學研究所碩士論文。4．劉姮昭1980．蘋果酸－乳酸醱酵菌的篩選及其對葡萄酒酸度之影響，國立臺灣大學植物科學研究所碩士論文。 5．劉曜東1975．檸檬酸醱酵生產之研究，Ⅱ．甘蔗糖蜜生產檸檬酸酵母菌之篩選，台灣糖業研究所審定發表論文第R7-48號，55-66。 6.蔣宏隆1971.利用鳳梨廢汁生產檸檬酸之研究，國立臺灣大學農業化學研究所碩士論文。7．蘇鴻基、楊昭明、林良平1961醱酵微生物實瞼法，P. 371-377 台津省菸酒公賣局。 8. Anderson, J.G. and J. E. Smith. 1971. The production of conidiophores and conidia by newly germinated conidia of Aspergillus niger (Microcycle conidiation). J. Gen. Microbiol. 69: 185-197. 9. Berry, D.R., A.Chmiel and Z.A.Obaidi. 1977. “Genetics and Physiology of Aspergillus”, p.405-426, ed. by Smith,J.E. and J.A. Pateman, Academic Press. l0.Bloom, S.J. and M.J.Johnson. 1962. The pyruvate carboxylase of Aspergillus niger. J. Biol. Chem. 237: 2718-2720. 11. Carpenter, P.L.1975. “Immunology and Serology”, 3rd. ed., p.305-307, The National Book Company Press. 12. Hang, Y. D., D. F. Splittstoesser and E. E. Woodams. 1977. Citric acid fermentation of brewery waste. J. Food Sci. 42: 383-384. 13. Heding, L.G. and J. K. Gupta. 1975. Improvement of conditions for precipitation of citric acid from fermentation mash. Biotechnol. Bioeng. 17: 1363-1364. 14. Kobayashi, T., G. V. Deden and M. Mooyoung. 1973. Oxygen transfer into mycelial pellets. Biotechnol. Bioeng. 15: 27-45. 15. Kubicek, C.P. and M. R?hr. 1978. The role of the tricarboxylic acid cycle in citric acid accumulation by Aspergillus niger. European J. Appl. Microbiol. Biotechnol. 5: 263-271. 16. Lockwood, L.B. 1975. “The Filamentous Fungi” 1:140-157, ed. by Smith, J.E. and D. R. Berry, Edward Arnold Press, London. 17. Messing, R.A. 1975. “Immobilization enzymes for industrial reactors” l8. Methods of enzymatic food analysis, 1981. Boehringer Mannheim Biochemicals, Indianapolis, Indiana. 19. Mori, T., T. Sato, T. Tosa and I. Chibata. 1972. Studies on immobilized enzymes X. Preparation and properties of aminoacylase entrapped into acrylamide gel-lattice. Enzymologia 43: 213-226. 20. M?hller, H.M. 1975. Oxalate accumulation from citrate by Aspergillus niger. Arch. Microbiol. 103: 185-189. 21. Natelson, S., J. B. Pincus and J. K. Lugovoy. 1948. Microestimation of citric acid; A new colorimetric reaction for pentabromoacetone. J. Biol. Chem. 175: 745-750. 22. Ohison, S., P.0. Larsson and K. Moshach. 1979. Steroid transformation by living cells immobilized by calcium alginate. European J. Appl. Microbiol. Biotechnol. 7: 103-110. 23. Perlman, D. ,W. W. Dorrell and M. J. Johnson. 1946. Effect of metallic ions on the production of citric acid by Aspergillus niger. Arch. Biochem. 11: 131-143. 24. Porges, N. 1932. Citric acid production by Aspergillus niger. Am, J. Botany 19: 559-567. 25. Prescott, S.G. and C. G. Dunn. 1959. Industrial Microbiology，p.533-577, 3rd ed. McGraw: Hill, New York. 26. Raper, K.B. and D. I. Fennell. 1973. “The Genus Aspergillus”, Robert E. Krieger Publishing Company Press. 27. Reed, G. 1981. “Prescott & Dunn's Industrial Microbiology”， p.709-747, 4th ed. McGraw Hill, New York. 28. R?hr, M. and C. P. Kubicek. 1981. Regulatory aspects of citric acid fermentation by Aspergillus niger. Process Biochem. June/July, 37-43. 29. Smith, J.E. and D. R. Berry. 1974. “An Introduction to Biochemistry of Fungal Development”, p.85-93, 30. Sodeck, G., J. Kominek and W. Salzbrunn. 1981. Production of citeic acid according to the submerged fermentation process. Process Biochem. October/November, 9-11. 31. Shu, P. and N. J. Johnson. 1948. Citric acid production by submerged fermentation with Aspergillus niger. Ind. Eng. Chem. 40: 1202-1205. 32. Symposium on immobilized microbial cells, p.l-1l, ed.by Venkatsubramarplan, K., 176th Annual Meeting of American Chemical Society, 1978. 33. Tomlinson, N., J. J. L. Champbell and P. C. Trussell. 1950. The inf1uence of zinc, iron, copper, and manganese on the production of citric acid by Aspergillus niger. J. Bacteriol. 59: 217-227. 34. Tosa, T. ,T. Sato, T. Mori, K. Yamamoto, I. Takata, Y. Nishida and I. Chibata. 1979. Immobilization of enzymes and microbial cells using carregeenan as matrix. Biotechnol. Bioeng. 21: 1697-1709. 35. Wold, W.S.M. and I. Suzuki. 1976. The citric acid fermentation by Aspergillus niger: regulation by zinc of growth and acidogenesis. Can. J. Microbiol. 22: 1083-1092. 36. Woronick, C.L. and M. J. Johnson. 1960. Carbon dioxide fixation by cell free extracts of Aspergillus niger. J. Biol. Chem. 235: 9-15. 37. Worthington Enzyme Mannual, l972. Worthington Biochem. Coop. Freehold. N. J. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75503 | - |
| dc.description.abstract | 利用Aspergillus aceutus TMB 2022 之分生孢子固定於calcium alginate之膠體上，進行檸檬酸醱酵。含2.32×10^8個分生孢子之懸浮液1 ml，包埋於Sodium alginate水溶液中，以製成3% Na-alginate (W/V),然後逐滴加入0.1 M CaCl2溶液中，膠體在室溫(20°-22℃)下靜置3小時，便形成3% Ca-alginate。將20 g 3% Ca-alginate之固定化分孢子培養在人工合成培養基，內含14%蔗糖，0.25% (NH4) 2S04, 15% KH2PO4，0.025% MgSO4•7H2O，另外加上0.03 mg/のCuSO4•5H2O, 0.5 mg/のZnCl2, 1.3 mg/のFeCl3•PH2O, PH 3.8。於35℃下進行表面培養，10天後可以獲得無水檸檬酸65.42 g/の，同樣條件下，在分批式培養，發現經固定後的分生孢子，可以維持較長的產酸期(31天)，無水檸綜酸產量從第2批到第4批均超過70 g/の，最高達77.02 g/の;對消耗糖收率約在65.65-69.83%之間。相反的，遊離分化孢子維持較短的產酸期，約17天，在分批式培養下，無水檸檬酸的最大收量為71.07 g/の(第2批)，但隨著批數的增加而檸檬酸量銳減。 | zh_TW |
| dc.description.abstract | Conidia of Aspergillus aculeatus TMB 2022 were immobilized in calcium alginate beads for use in the production of citric acid. A 1.0 ml conidia suspension containing about 2.32×10^8 conidia were entrapped into sodium alginate solution in order to prepare 3% Na-alginate(w/v), and then added drops to a 0.l M CaCl2 solution; the beads were allowed to stand at room temperature(20-22℃) for 3 hours to form 3% Ca-alginate. 20 grams of 3% Ca-alginate immobilized conidia were inoculated into synthetic medium containing 14% sucrose, 0.25% (NH4)2SO4, 0.15% KH2PO4, 0.025% MgSO4.7H2O with the addition of 0.03mg/l CuSO4.5H2O, 0.5mg/l ZnCl2, 1.3mg/l FeCl3.6H2O, pH 4.3. After fermentation at 35℃ for 10 days by surface culture, it can produce 65.42g/l anhydrous citric acid. Under the same condition at the batch-wise culture, it is found that immobilized conidia could maintain a longer period for citric acid production(31 days), over 70g/l of anhydrous citric acid at each run from No. 2 to No. 4, maximum yield for anhydrous citric acid reached 77.02g/l at run No. 2; in Contrast, free conidia maintained shorter period of acid producing phase, about 17 days, maximum yield for anhydrous citric acid was 7l.07g/l at run No. 2 but dropped down quickly as the run No. increased. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:33Z (GMT). No. of bitstreams: 0 Previous issue date: 1984 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………1 英文摘要……………………………………………3 Ⅰ緒言……………………………………………4 Ⅱ材料與方法……………………………………………12 一、檸檬酸生產菌之來源……………………………………………12 二、培養基……………………………………………12 三、菌種之鑑定……………………………………………14 四、菌種之保存……………………………………………14 五、分生孢子懸浮液之製備……………………………………………14 六、分生孢子數目之計算……………………………………………15 七、培養方法……………………………………………15 八、分析方法……………………………………………16 九、產酸率表示法……………………………………………21 十、分生孢子之固定……………………………………………21 十一、掃瞄式電子顯微鏡之觀察……………………………………………23 十二、實驗試藥……………………………………………24 Ⅲ結果……………………………………………25 一、各種檸檬酸定量法之比較……………………………………………25 二、沉澱法中各種回收處理之探討……………………………………………25 三、檸檬酸生產菌之節選……………………………………………30 四、菌種之鑑定……………………………………………30 五、各種固定化方法之比較……………………………………………33 六、固定化孢子之觀察……………………………………………36 七、硬化劑濃度對產酸之影響……………………………………………39 八、擔體濃度對產酸之影響……………………………………………39 九、接種量對產酸之影響……………………………………………42 十、膠體量對產酸之影響……………………………………………42 十一、固定化孢子之檸檬酸醱酵……………………………………………45 十二、培養溫度對固定化孢子產酸之影響……………………………………………48 十三、培養基之pH值對固定化孢子產酸之影響……………………………………………48 十四、不同碳源對固定化孢子產酸之影響……………………………………………52 十五、蔗糖濃度對固定化孢子產酸之影響……………………………………………52 十六、氮源對固定化孢子產酸之影響……………………………………………55 十七、磷酸二氫鉀添加量對固定化孢子產酸之影響…………………………………………55 十八、硫酸鎂添加量對固定化孢子產酸之影響……………………………………………58 十九、添加微量元素對固定化孢子產酸之影響……………………………………………58 二十、甲醇添加量對固定化孢子產酸之影響……………………………………………63 二十一、各種有利條件的配合……………………………………………65 二十二、分批式培養……………………………………………65 二十三、檸檬酸之定性分析……………………………………………69 Ⅳ討論……………………………………………75 Ⅴ參考文獻……………………………………………87 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用黑麴菌固定化分生孢子進行檸檬酸生? | zh_TW |
| dc.title | CITRIC ACID PRODUCTION USING IMMOBILIZED CONIDIA OF ASPERGILLUS ACULEATUS TMB 2022 | en |
| dc.date.schoolyear | 72-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 90 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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