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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Jih-Yau Chang | en |
dc.contributor.author | 張繼堯 | zh_TW |
dc.date.accessioned | 2021-07-01T08:13:25Z | - |
dc.date.available | 2021-07-01T08:13:25Z | - |
dc.date.issued | 1983 | |
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Gen. Virol. 7: 47-57. 38. Moss, L.H., and N. Gravell. 1969. Ultrastructure and Sequential Development of Infectious Pancreatic Necrosis Virus. J. Virol. 2: 52-58. 39. Nicholson, B. L. 1971. Macromolecule Synthesis in RTG-2 Cells Following Infection with Infectious Pancreatic Necrosis (IPN) Virus. J. Gen. Virol. 13: 369-372. 40. Nicholson, B. L., and C. Byrne. 1973. An Established Cell Line from the Atlantic Salmon(Salmo salar).. J. Fish. Res. Board Can. 30: 913-916. 41. Nicholson, B. L., and J. Dunn. 1974. Homologous Viral Interference in Trout and Atlantic Salmon Cell Cultures Infected with Infectious Pancreatic Necrosis Virus J. Virol. 14: 180-182. 42. Nicholson, B. L., G. W. Thorne, C. Janicki and A. Hanson. 1979. Studies on a Host Range Variant from Different Isolate5 of Infectious Pancreatic Necrosis Virus (IPNV). J. Fish Diseases 2: 367-379. 43. Nishimura, T., M. Toba, F. Ban, N. Okamoto, and T. Sano 1981a. Eel Rhabdovirus, EVA, EVEX and Their Infectivity to Fishes. Fish Pathol. 15: 173-184. 44. Nishimura, T.., H. Fukuda, H. Yamazaki, and T. Sano. 1981b.Concentration and Purification of Eel Virus, EVE. Fish Pathology 16: 75-83. 45. Nims, L., J. L. Fryer, and K. S. Pilcher. 1970. Studies of Replication of Four Selected Viruses in Two Cell Lines Derived from 5mb&dFjsh Proc. Sock Exp. Biol. Med. 135: 6-12. 46. Okamoto, N., T. Sano., R. P. Hedrick and J. L. Fryer. 1983. Antigenic Relationships of Selected Strains of Infectious Pancreatic Necrosis Virus and European Eel Virus. J. Fish Diseases6: 19-25. 47. Persson, R. H., and R. D. Macdonald. 1982. Evidence that Infectious Pancreatic Necrosis Virus Has a Genome-Linked Protein J. Virol 44: 437-443. 48. Pfitzner, I. & G. Schubert. 1969. Ein Virus aus dem Blut Mit Blumenkohlkrankheit Behafteter Aale. Z. Naturforsch. 24b: 790a-790b. 49. Reed, L, J. and H. Muench. 1938 A Simple Method of Estimating Fifty Percent Endpoints. Amer. Jour. Hyg.: 493_497. 50. Reich, E., R. N. Franklin, A. J. Shatkin, and E. L. Tatum, 1961. Effect of Actinomycin-D on Cellular Nucleic Acid Synthesis and Virus Production. Science 134: 556-557. 51. Rovozzo, G. C., and C. N. Burke. 1973. A Manual of Basic Virological Techniques. Prentice-Hall, Inc., Englewood Cliffs, N. J. pp. 87-93. 52. Salzman, N. P., A. J. Shatkin, and E.D. Sebring. 1964. The Synthesis of a DNA-like RNA in the Cytoplasm of Hela Cells Infected with Vaccinia Virus. Amer. Jour. Public Health.44: 563-570. 53. Sano, T. 1976. Viral Diseases of Cultures Fishes in Japan. Fish Pathol.10: 221-226. 54. Sano, T. N. Okanoto, and T. Nishimura. 1981 A New Viral Epizootic of Anguilla japonica Temminck and Schlegel. J. Fish Diseases 4: .127-139. . 55. Sidwell, R. W., J. H. Huffman, G. P. Khare, L. B. Allen, J. T. Witkowski, and R. K. Robins. 1972. Broad-spectrum Antiviral Activity of Virazole: 1-β-D-Ribofuranosyl- 1,2,4,-triazole-3-carboxamide. Science.177: 705-706. 6. Silverstein, S. C., J. K. Christman and G. Acs. 1976. The Reovirus Replicative Cycle. Ann. 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Role of Gene 59 of Bacteriophage T4 in Repair of UV-irradiated and alkylated DNA in vivo. J. Virol. 16: 5-16. .63. Tomasec,. J, and N. Fijan. 1971. Virusne Bolesti Riba (Viral Diseases of Fish). Final Report on Research under a Part of Project, 6n/1966, Zagreb. 64. Ueno, Y., and S. N. Chen. 1982. Studies of Virus Isolated from Japanese Eel (Anguilla Japonica) with. Nephroblastoma,, Institute of Zoology, National Taiwan University, Master Thesis, 65. Winton, .J R., C. N. Lannan, J. L. Fryer, and T. Kimura. 1981. Isolation of a New Reovirus from Chum Salmon in Japan. Fish Pathology 15 155-162. 66. Wolf, K., and N. C. Quimby. 1962. Established Eury-thermic Line .of Fish Cells in vitro. Science. 135: 1065-1066. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75478 | - |
dc.description.abstract | 由具有鰭部充血、體表潰爛、肝臟腫大壞死和體軀凹凸等病徵之日本鰻的肝臟、脾臟和腎臟,都可分離出病毒。利用細胞培養病毒,發現其在CHSE-214細胞中增殖最好。代謝抑制物FUDR和Actinomycin D試驗,結果顯示病毒之遺傳物質為核醣核酸。電子顯微鏡觀察下,病毒存在細胞質中靠近內質網處。負染色之純化病毒,顯示本病毒為不具外套膜,且祇含單層外套之二十面體,直徑約為59.8nm。在氯化銫中的浮力密度為1.33g/ml•SDS-polyacrylamide膠體電泳分析純化病毒之蛋白質和核醣核酸,顯示病毒顆粒含有分子量分別為90x10 3(VP 90),46x10 3(VP 46)和32x10 3(VP 32) daltons之蛋白質帶,以14C-uridine標示之病毒經電泳分析,由自動放射攝影中顯示出兩段核醣核酸。在低能量紫外線照射下,病毒之殘存率呈肩形曲線遞減,顯示其核醣核酸為雙股。中和試驗結果顯示其血清型和EVE(Eel Virus,European)密切相關,由以上結果證實本病毒屬於Birnaviridae病毒中的EVE病毒.但是進一步比較14C-amino acid標示之此分離病毒和Sano分離的EVE病毒的蛋白質,發現其在電泳膠體中的泳動距離和含量百分比略有不同。而利用紫外線照射和virazole處理抑制病毒增殖之實驗,發現在15,000 J.m-2劑量的紫外線下,可有效降低病毒感染力10 7TCID50/ml.1μg/ml劑量的virazole即可抑制病毒感染力10 4.5 TCID 50/ml。經由14C-uridine和14C-amino acid標示研究結果,virazole是抑制在病毒核醣核酸的合成。 | zh_TW |
dc.description.abstract | The virus was isolated from the liver, kidney and sp1 of diseased Japanese eels. Affected eels showed ulcer lesio over the body, congestion of fins, hypertrophy and necrosis of the liver, atrophic muscle and deformed trunk. Among the multiplication test of virus in various cell lines, we found that CHSE-214 cell line was the best one for viral multiplication. The metabolic inhibition test indicated that the viral nucleic acid was RNA. Electron microscopic observation revealed that the virus was presented in the cytoplasm near endoplasmic reticulum. It was unenveloped icosahedral, approximately 59.8 nm in diameter, and only had single layer capsid. The buoyant desity of the isolated virus in CsCl was 1.33 g/ml. By using polyacrylamide gel electrophoresis, three protein bands could be observed from the isolated virus, there were VP90 (M.W. 90,000 daltons), 46 (M.W. 46,000 daltons) and VP32 (M.W. 32,000 daltons). Autoradiograph showed that the virus possessed two segments of RNA genome. Under TJV light irradiation at low fluences, survial curve of virus displaied minor shoulder which revealed that the viral RNA was double stranded. The neutralization test indicated that the isolated virus had similar serotype with EVE (Eel Virus, European), and could be classified into EVE group of Binaviridae. But there were some differences in protein mobility and composition percentages between the isolated virus and EVE. The viral multiplication was inhibited by UV irradition and virazole treatment. Viral infectivity was reduced by 10 7 TCID50/ml under 15,000 J. m-2 tensity of UV irradiation, and was inhibited by 10 4.5TCID50/ml when 1 μg/ml of virazole was treated. Virazole inhibited the viral ribonucleic acid synthesis which was confirmed by using 14C-uridine and acid tracing. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:25Z (GMT). No. of bitstreams: 0 Previous issue date: 1983 | en |
dc.description.tableofcontents | 一、學位論文審查合格證明書. . . . . . . . . . . . . . . . . . . . i 二、縮寫表. . . . . . . . . . . . . . . . . . . . ii 三、摘要. . . . . . . . . . . . . . . . . . . . iv 四、謝辭. . . . . . . . . . . . . . . . . . . . vi 五、目錄. . . . . . . . . . . . . . . . . . . . vii 六、表次. . . . . . . . . . . . . . . . . . . . x 七、圖次. . . . . . . . . . . . . . . . . . . . xi 八、內容 第一章 緒論. . . . . . . . . . . . . . . . . . . . 1 第二章 材料與方法. . . . . . . . . . . . . . . . . . . . 7 2-1 魚類細胞株、初級細胞與病毒. . . . . . . . . . . . . . . . . . . 7 2-2 化學試劑. . . . . . . . . . . . . . . . . . . . 7 2-3 培養基與緩衝溶液. . . . . . . . . . . . . . . . . . . . 9 2-4 細胞之培養. . . . . . . . . . . . . . . . . . . . 10 2-5 病毒感染力之分析. . . . . . . . . . . . . . . . . . . . 11 2-6 鰻魚病變症狀. . . . . . . . . . . . . . . . . . . . 12 2-7 病毒之分離. . . . . . . . . . . . . . . . . . . . 13 2-8 病毒之增殖. . . . . . . . . . . . . . . . . . . . 14 2-9 病毒在不同細胞中之增殖. . . . . . . . . . . . . . . . . . . . 14 2-10 代謝抑制物試驗. . . . . . . . . . . . . . . . . . . . 15 2-11 病毒之純化. . . . . . . . . . . . . . . . . . . . 16 2-12 病毒密度之測定. . . . . . . . . . . . . . . . . . . . 18 2-13 電子顯微鏡之觀察. . . . . . . . . . . . . . . . . . . . 18 2-13-1 CHSE-214細胞內病毒顆粒之觀察. . . . . . . . . . . . 18 2-13-2 純化病毒之負染色. . . . . . . . . . . . . . . . . . . . 19 2-14 SDS-polyacrylamide平板膠體電泳. . . . . . . . . . . . . . . 20 2-14-1 蛋白質與核醣核酸之製備. . . . . . . . . . . . . . . . . . 20 2-14-2 放射性物質含量之測定. . . . . . . . . . . . . . . . . . . . 21 2-14-3 蛋白質與核醣核酸之電泳. . . . . . . . . . . . . . . . . . 22 2-14-4 苦抹藍染色. . . . . . . . . . . . . . . . . . . . 24 2-14-5 自動放射攝影. . . . . . . . . . . . . . . . . . . . 24 2-14-6 病毒蛋白質百分比之測定. . . . . . . . . . . . . . . . . 25 2-15 中和試驗. . . . . . . . . . . . . . . . . . . . 25 2-16 紫外線照射處理. . . . . . . . . . . . . . . . . . . . 26 2-17 Virazole抑制處理. . . . . . . . . . . . . . . . . . . . 27 2-18 Virazole處理對病毒核醣核酸合成之試驗. . . . . . . . 27 2-19 Virazole處理對病毒蛋白質合成之試驗. . . . . . . . . . 29 第三章 結果 3-1 病毒之分離. . . . . . . . . . . . . . . . . . . . 31 3-2 病毒在不同魚類細胞中之增殖. . . . . . . . . . . . . . . . 31 3-3 代謝抑製物試驗. . . . . . . . . . . . . . . . . . . . 37 3-4 病毒之純化. . . . . . . . . . . . . . . . . . . . 37 3-5 病毒之密度. . . . . . . . . . . . . . . . . . . . 39 3-6 電子顯微鏡之觀察. . . . . . . . . . . . . . . . . . . . 41 3-7 蛋白質與核醣核酸之電泳分析. . . . . . . . . . . . . . . . . 47 3-8 中和試驗. . . . . . . . . . . . . . . . . . . . 58 3-9 紫外線照射對病毒感染力之抑制. . . . . . . . . . . . . . 58 3-10 Virazole對病毒之抑制. . . . . . . . . . . . . . . . . . . . 61 3-11 Virazole處理對病毒核醣核酸和蛋白質合成之效應. . . . . . . . . . . . . . . 61 第四章 討論. . . . . . . . . . . . . . . . . . . . 69 九、參考文獻. . . . . . . . . . . . . . . . . . . . 78 十、英文摘要. . . . . . . . . . . . . . . . . . . . 85 | |
dc.language.iso | zh-TW | |
dc.title | 日本鰻所分離出兩段核醣核酸病毒之特性研究 | zh_TW |
dc.title | Characteristics of A Birnavirus Isolated from Japanese Eel (Anguilla japonica) | en |
dc.date.schoolyear | 71-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 86 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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