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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳俊宏 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:24Z | - |
| dc.date.available | 2021-07-01T08:13:24Z | - |
| dc.date.issued | 1983 | |
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Bull. Jap. Soc. Sci. fish., 44(5), 415-418. 41. watanabe, T., M. Sano, Y. Ishida, Y. Mizusawa and N. Michikawa (1980): Establishment and Properties of the Continuous Cell Line Derived from the Embryonated Eggs of Chum Salmon, Oncorhynchus keta. Bull. Jap. Soc. Sci. Fish., 46(10), 1203-1209. 42. Wolf, K. and C. E. Dunbar (1957): Cultivation of Adult Teleost Tissues in vitro. Proc. Soc. Exp. Biol. Med., 95, 455-458. 43. Wolf, K. and J. A. Mann (1980): Poikilothermic Vertebrate Cell Lines and Viruses: A Current Listing for Fishes. In Vitro, 16(2), 168-179. 44. Wolf, K. and M. C. Quimby (1962): Established Eurythermic Line of Fish Cells in vitro. Sci., 135, 1065-1066. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75475 | - |
| dc.description.abstract | 本研究之目的乃在於建立鯉魚及泥鰍之鰭細胞株，並研究此二細胞株之酵素染色及細胞表面顯微構造。 鯉魚及泥鰍鰭細胞在15個月間，各培養了105代和67代，並分別命名為CF及LF。此二細胞株最適宜的生長條件為含20％牛犢血清之Leibovitz'sL-15培養液，溫度在28?31℃之間。細胞表面附著效應值，LF為23?28％,CF為11?22%。LF及CF細胞皆為表皮樣細胞，CF細胞的細胞密度為120,000cells/平方公尺，而LF細胞則為48,000cells/平方公尺。CF及LF細胞株對於魚類病毒EVEX和EVA有感受性，而對其他包括EVE、IPNV、IHNV和VHSV等病毒無感受性。 CF及LF細胞在培養過程中，酸性磷酯?、鹼性磷醋?、腺?三磷醋?、琥珀酸去氫?、葡萄糖一六一磷酯?及細胞色素C氧化?等六種酵素的呈色反應會隨培養時間的增加而加深。其中以酸性磷酯?在CF細胞自體溶蝕時的反應最顯著。 CF及LF細胞在培養過程中，細抱外型會改變：CF細胞會有自發性的自禮溶蝕現象，而培養在37℃時，細胞會重疊且加速退化；LF細胞則由多樣化的外型趨於單一的多角型。這些細胞表面微細構造之連續變化情形，已分別由掃描電子顯微鏡，得到具體的觀察。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:24Z (GMT). No. of bitstreams: 0 Previous issue date: 1983 | en |
| dc.description.tableofcontents | 一、摘要…………………………………………Ⅰ 二、謝辭…………………………………………Ⅲ 三、目錄…………………………………………Ⅳ 四、內容…………………………………………1 第一章緒言………………………………………1… 第二章材料與方法………………………………5 第一節材料…………………………………………5 2.1-1、鯉魚及泥鰍……………………………………5 2.1-2、魚類細胞株及魚類病毒…………………5 2.1-3、磷酸?緩衝溶液…………………………5 2.1-4、組織洗滌液…………………………………………6 2.1-5、胰蛋白?溶液…………………………………………7 2.1-6、培養液…………………………………………7 2.1-7、受?質混合液…………………………………………8 第二節方法…………………………………………11 2.2-1、鯉魚鰭細胞之初級培養…………………………………………11 2.2-2、泥鰍鰭細胞之初級培養…………………………………………13 2.2-3、細胞繼代培養…………………………………………13 2.2-4、溫度對細胞生長速率之影響…………………………………………13 2.2-5、牛犢血清濃度對細胞生長速率之影響…………………………………14 2.2-6、不同培養液對細胞生長速率之影響……………………………14 2.2-7、細胞對溫度的忍受性…………………………………………14 2.2-8、CF及LF細胞之表面附著效應…………………………………15 2.2-9、CF及LF細胞對病毒的感受性…………………………………………16 2.2-10、CF及LF細胞內酵素染色…………………………………………16 2.2-11、掃描電子顯微鏡觀察…………………………………………17 2.2-12、污染檢驗…………………………………………18 第三章結果 3.1鯉魚及泥鰍鰭細胞之初級培養…………………………………………21 3.2細胞繼代培養…………………………………………21 3.3溫度對CF及LF細胞生長速率之影響…………………………………………22 3.4牛犢血清濃度對CF及LF細胞生長速率之影響……………………………23 3.5不同培養液對CF及LF細胞生長速率之影響…………………………………23 3.6CF及LF細胞對溫度之忍受性…………………………………………24 3.7CF及LF細胞之表面附著效應…………………………………………25 3.8CF及LF細胞對病毒之感受性…………………………………………25 3.9CF及LF細胞內酵素染色…………………………………………26 3.10掃描電子顯微鏡觀察…………………………………………27 3.11污染檢驗…………………………………………29 第四章討論…………………………………………30 圖表…………………………………………35 參考文獻…………………………………………57 | |
| dc.language.iso | zh-TW | |
| dc.title | 鯉魚和泥鰍鰭細胞株之建立及其生物學上之研究 | zh_TW |
| dc.title | Two Fish Cell Lines Derived from Carp Cuprinus carpio and Loach Misgunus anguillicaudatus Fins and Studies of Their Biological Characteristics | en |
| dc.date.schoolyear | 71-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 61 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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