請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75463完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 劉麗芳 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:20Z | - |
| dc.date.available | 2021-07-01T08:13:20Z | - |
| dc.date.issued | 1982 | |
| dc.identifier.citation | 1.Adhya,S.M.Gottseman,B.de—Crombrugghe, (1974). Release of polarity in Escherichia coil by gene N of phage laxmbda : termination and antitermination of transcription. Proc. Natl. Acad. Sci. U.S.,71,2534-2538. 2. Amemiya,K. ,B.Raboy,L.Shapiro, (1980). Involvement of the host RNA polymerase in the early transcription program of Caulobacter crescentus bacteriophage ?cdi DNA. Virology, 104,109-116 . 3. Ashley,R.D. ,J.Sambrook, (1980). Mapping viral mRNAs by sandwich hybridization . Methods in Enzymology, 65,468-478. 4. Brunovskis,I. ,W.C.Surnmers, (1972). The process of infection with Coliphage T7. VI. A phage gene controlling shutoff of host RNA synthesis. Virology,50,322-327. 5.Davis,R.U.,D.Botstein,J.R.Roth,(1980). A manual for genetic engineering :Advanced bacterial genetics • Cold Spring Harbor Laboratory,Cold Spring Harbor,New York, 11724, pp.234-235. 6. Denhardt,D.T.,(1966). A membrane-filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Cornmun.,23,641—646. 7.Falco,S.C.,K.V,Laan,L.B.Rothman-Denes,(1977). Virionassociated RNA polymerase required for bacterophage N4 development. Proc. Natl. Acda. Sci. U.S.,74,520—523. 8. Fox,T.D.,R.Losick,J.Pero.,(l976). Regulatory gene 28 of bacteriophage SPO1 codes for a phage-induced subunit of RNA polymerase. J. Mol. Biol.,10l,427-433. 9. Golomb,M. ,M.Chamberlin, (1974). A preliminary map of the major transcription units read by T7 RNA polymerase on the T7 and T3 bacteriophage chromosomes Proc. Nat. Acad. Sci. U.S.,71,760-764. 10. Golomb,M. ,M.Chamberlin, (1974). Characterization of T7— specific ribonucleic acid polymerase.IV. Resolution of the major in vitro transcripts by gel electrophoresis. J. Biol.Chem. ,249,2858-2863. 11. Haselkorn,R. M.Vo?ic1,R.D.Brown, (1969). Conservation of the rifampicin sensitivity to transcription during T4 development. Nature(London),221,836-838. 12. Hershowitz,I.,(1973). Control of gene expression in bacteriophage lambda. Annu. Rev. Genet. 7:289-324. 13. Hyman,R.W.,(1979). Physical mapping of T7 messenger RNA.,J. Mol. Biol.,6l,369—376. 14. Jolly,J.F.,(1979). Program of bacteriohage gh-l DNA transcription in infected Pseudomonas putida. J. Virol.,30,771-776. 15. Lean,K.V.,S.C.Falco,L.B.Rothman-Denes, (1977). The program of RNA synthesis in N4-infected Escherichia coli., Virology, 76, 596-601. 16. Laskey,R.A.,(l980). the use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods in Enzymology,65,363-37l. 17. Loening,U.E. (1969). the determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J.,113,13l—138. 18. Maritra,U.J.Hurwitz, (1965). The role of DNA in RNA synthesis,IX. Neucleoside triphosphate ternini in RNA polymerase products. Proc. Natl. Acad. 3d. U.S.,54, 815-822. 19. Naniatis,W.T.C.A.jeffery,D.G.Kleid, (1975).Nucleotide sequence of the rightward operator of phage. Proc. Natl. Acda. Sci. U.S.,72,1l04—l108. 20. Nca11ister,W.T. ,C.L.Barrett, (1977). Hybridization mapping of restriction fragments from the early region of bacteriophage T7 DNA, Virology,32,275-237, 21. Mca11ister,W.T. ,C.L.Barrett, (1977). Roles of the early genes of bacteriophage T7 in shutoff of host macromolecular synthesis. J. Virol.,23,543-553. 22. Mcallister,U.T. ,R.J.Mccarron, (1977). Hybridization of the in vitro products of bacteriophage T7 RNA polymerase to restriction fragments of T7 DNA. Virology,82,288-298. 23. Merril,C.R. ,r4.L.Dunau,D.Goldman, (1981). A rapid sensitive silver stain for polypeptides in polyacrylamide gels. Anal. Biochem. ,1lO,201—207. 24. Roberts,J.W.,(1969). Termination factor for RNA synthesis. Nature, 224,1168—1174. 25. Rothman-Denes,L.B.G.C.Schito, (1974). Novel transcribing activities in N4 infected E. coli.,Virology,60,65—72. 26. Salvo,R.A.,P.R.Chakraborty,U..iiaiter, (1’73). Studies on T3—induced ribonucleic acid polymerase IV. transcription of denatured deoxyribonucleic acid preparations by T3 ribonucleic acid polymerase. J. Biol. Chem.,248,6647—6654. 27. Southern,E.M.,(1975). Detection of specific sequences among DNA fragements separated by gel electrophoresis.J. Mol. Biol.,98,503-5l7. 28. Southern,E.M.,(1979). Gel electrophoresis of restriction fragments. Methods in Enzymology,68,l52-176. 29. Spiegelman,G.B.H.R.Whiteley,(1974). Purification of ribonucleic acid polymerase from SP82-infected Bacillus subtilis. J. Biol. Chem. ,249,1476-1482. 30. Studier,F.U.,(1973). Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J. Mol. Biol.,79,237-248. 31. Studier,F.W,J.V.Nailzel,Jr., (1969). T7-directed protein synthesis. Virology, 39, 575-586. 32. Suromers,W.C.,(1969). The process of infection with coliphage T7 I. Characterization of T7 FNA by polyacrylamide gel electrophoretic analysis. Virology,39,175-182. 33. Summers,W.C. ,I.BrunovskiI, R.W.Hyman, (1973). The process of infection with coliphage T7. VII,Characterization and mapping of the major in Viyo transcription products of the early region.J. Mol.Biol.,74,291-300. 34. Summers,W.C. ,R.G.Siegel, (1970). Transcription of latephage RNA by T7 RNA polymerase. Nature,228,1160-1162. 35. Summers,W.C.,R.G.Siegel,(l969). Control of template specificity of E. coli.RNA polyrnerase by a phage-coded protein. Nature(London) ,223,ll1l-l113. 36. Switzer,R.C. III,C.R.Merril,S.Shifrin, (1979). A highly sensitive silver stain for deteting proteins and peptides in polyacrylamide gels. Anal. Biochem.,98,231-237. 37.Tove1,H.C.,J.F.Jolly,J.A.Boczi, (1975). Purification and characterization of bacteriophage gh-1-induced deoxyribonucleic acid-dependent ribonucleic acid polymerase from seudomonas putida. J. Biol. Chem.,250, 1723—1733 38 ．戴華、江昆生、郭宗德，( 1976）。柑桔潰瘍病病原細菌的綿狀噬菌體 C f 。總統蔣公逝世周年紀念論文集， 411 － 425 頁。中央研究院出版。 39 ．廖有地、郭宗德， ( 19 79 。噬菌體 X p 10 發育過程中核醣核酸聚合?之誘導和改變。師範大學生物研究所碩士論文。 40 ．宋德雯、郭宗德， ( 1980）。噬菌體 X p 10 感染之 Xanthomonas oryzae 中寄主 RNA 聚合?之抑制劑。師範大學生物研究所碩士論文。 41 ．施麗貞、郭宗德， ( 1981 ）。噬菌體 X p 10 所誘導負責關閉寄主細胞轉錄的抑制劑。台灣大學植物研究所碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75463 | - |
| dc.description.abstract | 中文摘要 為瞭解噬菌體 X p 10 感染寄主後在寄主細胞中轉錄的情形，曾在生體及試管的條件下，探求其轉錄的產物－ mRNA ，並進一步的利用 Southern 的方法探求其基因轉錄的安排。在生體條件的試驗中，將生體內的 X p 10 mRNA 以“ H － uridine 標幟，經 hot phenol 及酒精萃取，以 polyacrylamide 凝膠電泳分析法及凝膠放射活性測定法分析，得知其早期基因之轉錄產物可以產生 5 種 mRNA ，其中有一種會迅速被分解；中、晚期基因之轉錄開始於感染後 10 － 20 分鐘之間，可以產生 6 種 mRNA 。其中似乎尚有早期基因的 mRNA ，其原因可能是由於早期基因轉錄產物中的一個負責抑止寄主 RNA 聚合?之抑制劑作用不完全，或是新誘導的轉錄系統亦有轉錄早期基因的能力。 利用感染菌中純化出來的噬菌體 RNA 聚合?及感染前、後之寄主 RNA聚合?，於試管中進行轉錄，用凝膠放射活性測定法分析，發現感染後的寄主 RNA 聚合酪活性很低，原寄主 RNA 聚合?可產生 4 種，而噬菌體所誘導的 RNA 聚合?產生 3 種 mRNA 。此結果與生體條件下做出來的不甚相同。這種差異可能很正常，因為在生體條件下有更複雜的調節系統存在，而在試管條件下作用較單純，故一定會有所差別。為進一步瞭解基因表現的安排，另用 Southern 的 DNA 和 RNA 雜交分析法，將 X p l0 感染細胞內用 32P 標幟的 m R NA 與 nitrocellulose membrane 上經各種不同核酸鑑識?切成的 X p 1 0 DNA 片段進行雜交，因在方法上尚有待改善之處，故未得良好結果。 | zh_TW |
| dc.description.abstract | In order to understand the transcription Xpl0 phage on one in infected ce11, the transcription products—mRNA crc ctudicd with gel electrophoresis, and the gene arrange— lent of Xpl0 genome were also explored with Southern blotting nalysis. In vivo study phage infected cells were fed with H-uridine, and mRNA was extracted with phenol and precipitated with ethanol, and the labeled mRNA were analyzed with gel electro) horesis. For early gene 3 species of mRNA was detected, among these, one mRNA was rather unstable. For late gene 6 species of mRNA were obtained. Among these, early gene transcripts were also detected. In vitro study the purified phage - specific RNA polymerase was used and Xpl0 DNA used as template. For the comparison purified host RNA polymerase and modified host RNA polymerase were also used. Phage induced RNA polymerase produced 5 species of mRNA, host RNA polymerase produced 4 species of mRNA, and modified host RNA polymerase produced nothing. Based on the size of mRNA species the pattern of in vitro system was completely different from that of in vivo system. In order to explored the arrangement of gene expression, the Southern blotting analysis method was developed. The whole system seems to working well. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:20Z (GMT). No. of bitstreams: 0 Previous issue date: 1982 | en |
| dc.description.tableofcontents | I 言緒 . . . . . . . . . . . . . . 1 II.材料與方法. . . . . . . . . . . . . 9 III.結果. . . . . . . . . . . . . 26 一、 生體內 mRNA 之研究. . . . . . . . . . . . . 26 1. Xpl0DNA 分子量之測定 . . . . . . . . . . . . . 26 2. 生體內 mRNA 在 polyacrylamide 凝膠上之電泳分析 . . . . . . . . . . . 28 二、試管中合成之mRNA 在polyacrylamide 凝膠上之電泳分析 . . . . . . . 33 三、以Southern氏圖法分析生體內之 mRNA . . . . . . . . . . . 36 1. DNA 片段轉移到nitocellu1ose membrane . . . . . . . . . . . 36 2. DNA 與生體內mRNA之核酸雜交 . . . . . . . . . . . 38 3. Human /3j- globulin gene DNA － DNA 核酸雜交. . . . . . . . . . . 43 VI．討論. . . . . . . . . 45 V .參考資料 . . . . . . . . . 55 | |
| dc.language.iso | zh-TW | |
| dc.title | 噬菌體XP10基因體轉錄之研究 | zh_TW |
| dc.title | Studies on transcription of phage Xp10 genome | en |
| dc.date.schoolyear | 70-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 66 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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