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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.author | Shau-Chi Chi | en |
| dc.contributor.author | 齊肖琪 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:13Z | - |
| dc.date.available | 2021-07-01T08:13:13Z | - |
| dc.date.issued | 1982 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75440 | - |
| dc.description.abstract | 本報告乃在?述有關吳郭魚卵巢細胞株To-2之建立。此細胞株在添加了10%牛犢血清之Lei-bovitz’s L-15培養液中，於31±1℃的條件下已繼代培養了75次。To-2細胞株的形態為單核及多核之表皮樣細胞。分析100個分裂中期To-2細胞的染色體數目，結果顯示染色體數目的分佈範圍自24至76呈雙向分佈，而以含40個染色體之細胞最多。於低密度細胞培養時，細胞表面附著效應(Plating Effeciency)約12.5%。比較To-2細胞在含2%,5%,10%,20%等四種牛犢血清濃度的培養液中之生長情況，結果顯示其生長速率隨著血清濃度的升高而增高。To-2細胞可生長的溫度範圍自15℃至37℃，而於31℃的條件下生長情況最好。本實驗亦證實了To-2細胞株可接受EVA、EVE、EVEX及IPNV四種病毒，且EVE及IPNV在To-2細胞內之複製情形良好。To-2經鑑定沒有細菌、真菌及黴菌質(Mycoplasma)的污染。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:13Z (GMT). No. of bitstreams: 0 Previous issue date: 1982 | en |
| dc.description.tableofcontents | 一、謝辭. . . . . . . . . . . . . . . . . . . . . . . . . . i 二、目錄. . . . . . . . . . . . . . . . . . . . . . . . . . ii 三、摘要. . . . . . . . . . . . . . . . . . . . . . . . . . v 四、內容 第一章 緒言. . . . . . . . . . . . . . . . . . . . . . . . . . 1 第二章 材料與方法. . . . . . . . . . . . . . . . . . . . . . . . . 4 2.1 材料 2.1.1 吳郭魚. . . . . . . . . . . . . . . . . . . . . . . . 4 2.1.2 胰蛋白溶液. . . . . . . . . . . . . . . . . . . . . . . . 4 2.1.3 培養液. . . . . . . . . . . . . . . . . . . . . . . . 5 2.1.4 Giemsa染料溶液. . . . . . . . . . . . . . . . . . . . . . . 6 2.1.5 電子顯微鏡標本製備試劑. . . . . . . . . . . . . . . . 6 2.1.6 病毒來源. . . . . . . . . . . . . . . . . . . . . . . . 8 2.1.7 TSA培養基. . . . . . . . . . . . . . . . . . . . . . . . 8 2.1.8 Mycoplasma Stain Kit . . . . . . . . . . . . . . . . . . . . 9 2.1.9 Carnoy’s固定液. . . . . . . . . . . . . . . . . . . . . . . . 9 2.1.10 儀器及溶液的消毒. . . . . . . . . . . . . . . . . . . . . 9 2.2 方法 2.2.1 吳郭魚初級細胞培養之建立. . . . . . . . . . . . . 10 2.2.2 繼代培養. . . . . . . . . . . . . . . . . . . . . 11 2.2.3 染色體數目之分析. . . . . . . . . . . . . . . . . . . . . 11 2.2.4 牛犢血清濃度對細胞生長速率之影響. . . . . 12 2.2.5 細胞表面附著效應. . . . . . . . . . . . . . . . . . . . . 13 2.2.6 細胞貯存. . . . . . . . . . . . . . . . . . . . . 14 2.2.7 不同溫度對細胞生長速率之影響. . . . . . . . . 14 2.2.8 細胞對病毒感受性試驗. . . . . . . . . . . . . . . . 15 2.2.9 污染鑑定. . . . . . . . . . . . . . . . . . . . . 18 第三章 結果 3.1 吳郭魚初級細胞培養之建立. . . . . . . . . . . . . 20 3.2 繼代培養. . . . . . . . . . . . . . . . . . . . 20 3.3 染色體數目之分析. . . . . . . . . . . . . . . . . . . . . 21 3.4 牛犢血清濃度對細胞生長速率之影響. . . . . . 21 3.5 細胞表面附著效應. . . . . . . . . . . . . . . . . . . . . 22 3.6 細胞貯存. . . . . . . . . . . . . . . . . . . . . 22 3.7 不同溫度對細胞生長速率之影響. . . . . . . . . 22 3.8 細胞對病毒感受性試驗. . . . . . . . . . . . . . . . . 23 3.9 細胞污染鑑定試驗. . . . . . . . . . . . . . . . . . . . . 24 第四章 討論. . . . . . . . . . . . . . . . . . . . . 39 五、文獻. . . . . . . . . . . . . . . . . . . . . 44 | |
| dc.language.iso | zh-TW | |
| dc.title | 吳郭魚卵巢細胞株之建立 | zh_TW |
| dc.title | A Cell Line Derived from Tilapia (Tilapia mossambica X Tilapia nilotica) Ovary | en |
| dc.date.schoolyear | 70-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 47 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| Appears in Collections: | 動物學研究所 | |
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