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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Ming-Sheng Hsu | en |
| dc.contributor.author | 徐明聖 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:08Z | - |
| dc.date.available | 2021-07-01T08:13:08Z | - |
| dc.date.issued | 2003 | |
| dc.identifier.citation | 李孟惠．溫度、光度及肥料濃度對文心蘭花序發育之影響．國立臺灣大學園藝學研究所碩士論文．1997． 李哖．台灣農家要覽農作篇．1995． 李哖、李嘉慧．蝴蝶蘭花芽誘引和花序發育時之碳水化合物變化．中國園藝．1996． 李泰昌．鈣肥濃度和通風處理對文心蘭生育與開花品質之影響．國立中興大學園藝學系碩士論文．1998． 沈碧君、李哖．春化作用與GAs對植物抽苔開花的影響．中國園藝．1983．29(4):243-249． 徐懷恩．不同光照，氮源屋料及花梗修剪對文心蘭開花之影響．國立中興大學園藝學系碩士論文．1996． 張允瓊．溫度光度及肥料濃度對文心蘭生長與開花之影響．國立臺灣大學園藝學研究所碩士論文．1995． 張允瓊、李哖．溫度對文心蘭（Oncidium Gower Ramsey）假球莖生長及花序發育之影響．中國園藝．2000．46(2):221-230． 張清安．蘭花病毒病害．2001． 蔡佩芬．溫度、光度、栽培介質及肥料濃度對文心蘭苗生育之影響．國立臺灣大學園藝學研究所碩士論文．1999． Aharoni A, Keizer LC, Bouwmeester HJ, Sun Z, Alvarez-Huerta M, Verhoeven HA, Blaas J, Van Houwelingen AM, De Vos RC, Van Der Voet H, Jansen RC, Guis M, Mol J, Davis RW, Schena M, Van TAJ, O'Connell AP. Identification of the SAAT gene involved in strawberry flavor biogenesis by use of DNA microarrays. Plant Cell. 2000. 12:647-662. Aoki K, Kragler F, Xoconostle-Cazares B, Lucas WJ. A subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75424 | - |
| dc.description.abstract | 文心蘭開花期的決定似乎與其營養狀態有相當大的關聯；而假球莖是文心蘭貯藏水分及養分的主要器官，本論文即以扣減式基因庫結合cDNA微陣列晶片技術，以二種不同發育階段：A時期，花軸2?3公分；及B時期，花軸7?8公分之文心蘭假球莖為材料進行扣減式篩選，期望能夠得到影響開花時間之基因。在A減B扣減式基因庫中，獲得了45個在A時期表現倍數大於B時期2倍的cDNA片段序列，在比對已知蛋白質序列的結果顯示，11個基因產物與光合作用相關，另外11個基因可能參與了蛋白質的合成分解及運送，其他還包括了似植物乳膠蛋白（latex-like proteins）、過氧化物?（peroxidases）、甘露糖結合活性的植物凝集素（mannose-binding lectins）等。本論文並對其中之一的過氧化物脢，在分類上屬於植物第三群（class III），初步探討其在開花過程中所扮演的角色。 | zh_TW |
| dc.description.abstract | The pseudobulb of Oncidium Gower Ramsey is the primary storage organ for water and nutrients preservation. The determination of flower trigger of Oncidium seems to have a strong relationship with the nutritional status of pseudobulbs. In order to obtain the genes specifically involved in the determination of flower trigger of Oncidium, we used PCR-select cDNA subtraction and cDNA microarray techniques to screen genes differentially expressed between two different developmental stages of pseudobulbs. Stage A represented the pseudobulbs with floral bud of 2?3 cm length, and stage B represented the pseudobulbs with floral bud of 7?8 cm length. We obtained 45 cDNA clones that expressed two folds higher in the A stage than those in the B stage, after screening the A minus B subtraction library. There are 11 clones found to be involved in photosynthesis process, and another 11 clones were found to be involved in protein synthesis and transportation. The others are latex-like proteins, peroxidases and mannose-binding lectins. On the other hand, we study further one of the peroxidases gene, OPOXl, which belongs to plant class III peroxidase. The gene expressions of OPOXl in Oncidium during different developmental stages were investigated, and the role of OPOXl involved in the flowering process has been discussed. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2003 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………2 英文摘要………………………………………………………………3 前言……………………………………………………………………4 材料與方法……………………………………………………………17 結果……………………………………………………………………47 討論……………………………………………………………………56 圖表……………………………………………………………………63 參考文獻………………………………………………………………77 附錄……………………………………………………………………86 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用扣減法與基因微陣列技術篩選文心蘭假球莖中花序發育初期專一表現之基因 | zh_TW |
| dc.title | Using cDNA microarray techniques to select differentially expressed genes from pseudobulb during initial floral development of Oncidium Gower Ramsey | en |
| dc.date.schoolyear | 91-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 95 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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