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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Tsung-Yang Hsu | en |
| dc.contributor.author | 徐綜遠 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:03Z | - |
| dc.date.available | 2021-07-01T08:13:03Z | - |
| dc.date.issued | 2003 | |
| dc.identifier.citation | Brugnera E, Haney L, Grimsley C, Lu M, Walk SF, Tosello-Trampont AC, Macara IG, Madhani H, Fink GR, Ravichandran KS. (2002) Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. Nat Cell Biol. 2002 Aug;4(8):574-82. Cote JF, Vuori K. (2002). Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity. J Cell Sci. 2002 Dec 15;115(Pt 24):4901-13. Ellis, R., Jacobson, D. M., and Horvitz, H. R. (1991). Genes required for the engulfment of cell corpses during programmed cell death in C. elegans. Genetics 129, 79-94. Fadok, V. A., Bratton, D. L., Rose, D. M., Pearson, A., Ezekewitz, R. A., and Henson, P. M. (2000). A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature 405, 85-90. Fadok, V. A., McDonald, P. P, Bratton, D. L., and Henson, P. M. (1998). Regulation of macrophage cytokine prodution by phagocytosis of apoptotic and post-apoptotic cells. Biochem Soc Trans 26, 653-6. Hanayama R, Tanaka M, Miwa K, Shinohara A, Iwamatsu A, Nagata S. (2002) Identification of a factor that links apoptotic cells to phagocytes. Nature. May 9;417 (6885):182-7. Hanahan, D., and Weinberg, R. A. (2000). The hallmarkers of cancer. Cell 100, 57-70. Horvitz, H. R. (1999). Genetic control of programmed cell death in the nematode Caenorhabditis elegans. Cancer Res 59,1701s-1706s. Jacobson, M. D., Weil, M., and Raff, M. C. (1997). Programmed cell death in animal development. Cell 88, 347-54. Nakano, T., Ishimoto, Y., Kishino, J., Umeda, M., Inoune, K., Nagata, K., Ohashi, K., Mizuno, K., and Arita, H. (1997). Cell adhesion to phosphatidylserine mediated by a product of growth arrest- specific gene 6. J Biol Chem 272, 29411-4. Scott, R. S., Mcmahon, E. J., Pop, S.M., Reap E. A., Caricchio, R., Chohen, P. L., Ear, H. S., and Matsushima, G. K. (2001). Phagocytosis and clearance of apoptotic cells is mediated by MER. Nature 411, 207-11 Sulston, J. E., and Horvitz, H. R. (1977). Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Dev Biol 56, 110-56. Vijay Yajnik, Charles Paulding, Raffaella Sordella, Andrea I. McClatchey, Mako Saito, Doke C.R. Wahrer, Paul Reynolds, Daphne W. Bell, Robert Lake, Sander van den Heuvel, Jeff Settleman, and Daniel A. Haber (2003). DOCK4, a GTPase Activator, Is Disrupted during Tumorigenesis. Cell 2003 112: 673-684. Wu, Y. C., and Horvitz, H. R (1998). The C. elegans cell corpse engulfment gene ced-7 encodes a protein similar to ABC transporters. Cell. 1998 Jun 12;93(6):951-60. Wu, Y. C., and Horvitz, H. R (1998). C. elegans phagocytosis and cell-migration protein CED-5 is similar to human DOCK180. Nature. 1998 Apr 2;392(6675):501-4. Wu YC, Tsai MC, Cheng LC, Chou CJ, Weng NY. (2001). C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. Dev Cell. 2001 Oct;1(4):491-502. Zhou Z, Caron E, Hartwieg E, Hall A, Horvitz HR The C. elegans PH domain protein CED-12 regulates cytoskeletal reorganization via a Rho/Rac GTPase signaling pathway. Dev Cell. 2001 Oct;1(4):477-89 Zhou Z, Hartwieg E, Horvitz HR. CED-1 is a transmembrane receptor that mediates cell corpse engulfment in C. elegans. Cell. 2001 Jan 12;104(1):43-56. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75408 | - |
| dc.description.abstract | 細胞的自然性死亡，apoptosis或programmed cell death發生於多細胞的生物體內，訊號機制啟動時會避免發炎反應的產生與自體免疫的產生，以降低對生物體的傷害。利用線蟲（Caenorhabditis. elegans）為模式動物，細胞凋亡的形態學特徵包括有：染色質濃縮（pyknosis）、細胞質濃縮、細胞形態變得不規則而且有段裂（fragmentation）、及由凋亡細胞脫落出細胞凋亡體（apoptotic body）研究細胞計劃性死亡是指活體生物中的某些特定細胞會在特定的時間與地點會進行細胞死亡，而這種細胞死亡的過程是由生物體內的基因來控制，而細胞死亡時的屍體被吞噬細胞或臨近細胞所吞噬。 在此探討於物種間為高度保守基因的phosphatiylserine receptor(PSR)與receptor tyrosine kinase, MER；以線蟲（C. elegans）基因，c-psr與c-mer為目標基因。在之前的遺傳學研究中發現，於活體內中將c-psr mutation的背景中，將已知的CED-2/CrkII、CED-5/Dock180、CED-10/Rac過量表現，其可以援救細胞吞噬作用的缺失。而在非活體的條件中，實驗發現PSR會與CED-5和CED-12結合，因此推論cPSR可能為該訊號傳遞路徑的接受器。另一高度保守基因，receptor tyrosine kinase-----cMER，其座落於吞噬細胞（phagocyte），細胞膜表面的接受器，在與c-psr突變的實驗中，發現有相同的實驗結果；推論其一樣參與相同的訊息路徑，影響吞噬作用中細胞骨架的移動。今探討PSR與MER蛋白之間是否有相互作用，而形成heterodimmer的型式；PSR的磷酸化作用產生是否受到MER的參與。另外以遺傳學方式尋找線蟲（C. elegans）體中，是否有其他新的tyrosine kinase receptor也參與細胞吞噬作用。 | zh_TW |
| dc.description.abstract | The apoptosis or programmed cell death is the physical mode to remove unwanted cells by specific phagocytes and non-professional phagocyte. It is to avoid inflammation and autoimmunity. When apoptosis proceed; the macrophages are recognized phosphatidylserine, which was sent to out site of membrane of apoptotic cells. In our lab research, the in vivo genetic result that c-psr mutant background will reduce cell engulfment function, but in overexpression condition; the CED-2/CrkII, CED-5/Dock180, CED-10/Rac-1 and CED-12/ELMO-1 could rescue defect phenotype. And in vitro experiments, cPSR will interaction with CED-5. Then we suggest that c-psr take part in this signal pathway to affect cell cytoskeleton morphology. And receptor tyrosine kinase, cMER, in double mutant condition also have the same result with c-psr, so it think that c-mer also regulate CED-2/CrkII, CED-5/Dock180, CED-10/Rac-1, CED-12/ELMO-1 signal pathway, affect cytoskeleton arrangement. The gold is detected psr and mer relationship, observe whether have protein-protein interaction or not; And research whether RTK receptor take part in cell engulfment mechanism or not. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2003 | en |
| dc.description.tableofcontents | 壹：中文摘要…………………………………………………………1 貳：Abstrate…………………………………………………………3 參：導言 一．細胞凋亡（apoptosis）………………………………………4 二．凋亡小體（apoptotic bodies的吞噬作用）…………………5 三．線蟲中細胞屍體的吞噬作用……………………………………7 肆：結果 一．psr與mer在細胞吞噬功能性…………………………………12 二．c-psr和c-mer之間的protein-protein interaction………13 三．PSR的tyrosine磷酸化機制的產生……………………………14 四．遺傳學方法搜尋其他Receptor Tyrosine Kinase…………16 伍：討論……………………………………………………………17 陸：方法與材料……………………………………………………19 柒．參考文獻：……………………………………………………22 捌．附圖與表格……………………………………………………25 | |
| dc.language.iso | zh-TW | |
| dc.title | 探討線蟲（C. elegans）接受器cPSR和cMER以及找尋有關吞噬作用的tyrosine kinase接受器 | zh_TW |
| dc.title | Biochemical analysis of phagocytotic receptors cPSR and cMER and screen for receptor tyrosine kinases for new phagocytotic receptors in C. elegans | en |
| dc.date.schoolyear | 91-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 33 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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