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標題: | 荷花B群花部同源基因之選殖興特性研究 Isolation and Characterization of B-Class Floral Homeotic Genes in Nelumbo nucifera (Nelumbonaceae) |
作者: | Cheng-Chiang Wu 吳正江 |
出版年 : | 2003 |
學位: | 碩士 |
摘要: | 本研究從荷花(Nelumbo nucifera)選殖出兩個B群花部同源基因NnPI與NnAP3,並藉由基因表現模式與分子譜系分析探討荷花花被發育演化之遺傳機制。本研究中所得到的NnPI片段具有748個鹼基對,涵蓋MADS domain、I domain、K domain、C domain,與3'端非轉譯區域,共包含可轉譯為186個胺基酸的區域。NnPI的C端具有一個PI motif。NnAP3片段則具有744個鹼基對,涵蓋MADS domain、I domain、K domain、C domain,與3'端非轉譯區域,包含可轉譯為200個腰基酸。NnAP3的C端具有一個PI-derived motif與一個paleoAP3 motif。在使用M-I-K-C區域序列以進行的最高簡約原理、近鄰結合,與最大可能性等三種分子譜系分析中,NnPI與NnAP3大多與來自真雙子葉基群植物的B群花部同源基因有較近之親源關係,但支持度並不高。基因表現分析顯示這兩捆基因的表現模式與真雙子葉植物內的B群花部同源基因有所出入。在內花被與雄蕊中可以偵測到NnPI的表現,而NnAP3的表現則可以在外花被、內花被、雄蕊,與心皮中被偵測到。因此,NnPT與NnAP3的表現形式也許反映了B群花部同源基因在真雙子葉植物分化之前在表現區域與功能的彈性。 Two B-class floral homeotic genes, NnPI and NnAP3 were identified from Nelumbo nucfera (Nelumbonaceae) and characterized in this study. The obtained cDNA of NnPI was 748 bp in length with a PI-motif in the C-terminal region of its 186 amino acids predicted. The partial NnAP3 was 744 bp with a PI-derive motif and a paleoAP3 motif in the C-terminal region of its 200 amino acids predicted. The most parsimony, neighbor-joining, and maximum-likelihood analyses based on their nucleotide and amino acid sequences of the M-I-K-C domain revealed that NnPI and NnAP3 were grouped with other basal eudicot B-class lineages with low bootstrapping support. This is congruent with current phylogeny regarding the systematic position of N. nucifera. Expression analysis showed that the expression patterns of the two identified genes do not match exactly with the B-class genes of core eudicots. NnPI was expressed in the inner perianth and stamens, while NnAP3 was expressed in the outer perianth, inner perianth, stamens, and carpels. Therefore, the expression pattern of NeAP3 and NnPI may reflect the plasticity of B-class gene expression and function before the core eudicot lineages. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75365 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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