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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Chih-Ying Chao | en |
| dc.contributor.author | 趙芷瑩 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:50Z | - |
| dc.date.available | 2021-07-01T08:12:50Z | - |
| dc.date.issued | 2003 | |
| dc.identifier.citation | 李文靜．2002．蝴蝶蘭屬植物核醣體RNA基因的選殖及實質定位．國立台灣大學植物科學研究所碩士論文．
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75359 | - |
| dc.description.abstract | 17?18S-5.8S?25?28S rDNA為聚集型的重複性序列，每個重複單位包含轉錄區，ITS及IGS。其中ITS序列長度短，在物種間差異很大，且其兩側具高度保留性的轉錄區很容易擴增，因此常用於物種間親緣關係的分析。本實驗以P.aphrodite之18S和26S基因轉錄區設計一對專一性的引子，利用聚合酵素連鎖反應擴增28種蝴蝶蘭及1種朵麗蘭植物之ITS序列，分析種間的親緣關係。由con-NJ樹可將植物區分成6群，其中第2群包括P.stobartiana和P.parishii，第3群包括P.lued-pulehra、P.pallens和P.fasciata，皆與5S rDNA之IGS序列分析結果一致。另外P.amboinensis、P.venosa和P.violacea屬於同一群，與核型觀察及5S rDNA之IGS序列分析結果一致。而P. equestris、P.amabilis、P.aphrodite、P.sanderiana、P.stuarttana和P.schilleriana具小型染色體的蝴蝶蘭區分為三群，與5S rDNA之IGS序列分析的結果並不一致，可能是同一種植株因種源不同，導致ITS序列呈多型性所致。 | zh_TW |
| dc.description.abstract | l7?l8S-5.8S-25S rDNA are organized into clusters of tandem repeat with each repeat consisting of transcribed regions, two small ITS, and an IGS. Because the small size and the presence of highly conserved sequences flanking each of the two ITS make this region easy to amplify. The ITS sequences are variable among species and useful for phylogenetic analyses. In this study, ITS of 28 Phalaenopsis species were amplified by the polymerase chain reaction, cloned, and sequenced. Phylogenetic analysis of ITS consensus sequences could be divided into six groups. Among them the second group containing P.stobartiana and P.parishii and the third group containing P. lued-pulchra, P. pallens, and P. fasciata were consistent with IGS of 5S rDNA analysis. Similarly, P. amboinensis, P. venosa and P. violacea were also grouped together. Whereas P. equestris, P. mabilis, P. aphrodite, P. sanderiana, P. stuartiana, and P. schilleriana were divided into three groups, it might be the result of polymorphism in ITS sequences from different source of plants. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2003 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………………………………………………………………i 英文摘要………………………………………………………………………………………………………………………ii 簡寫字對照表…………………………………………………………………………………………………………………iii 壹、前言………………………………………………………………………………………………………………………1 貳、材料與方法 一、植物材料……………………………………………………………………………………………………………7 二、DNA的抽取 …………………………………………………………………………………………………………7 三、ITS序列的擴增 ……………………………………………………………………………………………………8 四、探針的標定…………………………………………………………………………………………………………9 五、選殖體的篩選………………………………………………………………………………………………………10 六、ITS序列之定序及分析 ……………………………………………………………………………………………13 七、親緣關係分析………………………………………………………………………………………………………13 參、結果 一、185-5.85-26S rDNA之ITS序列的選殖及定序……………………………………………………………………15 二、蝴蝶蘭屬植物ITS序列的比較 ……………………………………………………………………………………15 三、親緣關係之分析……………………………………………………………………………………………………16 肆、討論………………………………………………………………………………………………………………………19 伍、參考文獻…………………………………………………………………………………………………………………22 圖次 圖1、依蝴蝶蘭P.aphrodlte 18S-5.8S-26S rDNA的基因序列設計兩個引子之位置及序列………………………28 圖2、蝴蝶蘭屬植物和一種朵麗蘭屬植物之ITS序列選殖體的篩選…………………………………………………29 圖3、P.lowii五個選殖體序列之對齊排列……………………………………………………………………………30 圖4、P.stobartiana五個選殖體序列之對齊排列……………………………………………………………………31 圖5、P.fuscata五個選殖體序列之對齊排列…………………………………………………………………………32 圖6、P.viridis五個選殖體序列之對齊排列…………………………………………………………………………33 圖7、P.amabilis五個選殖體序列之對齊排列 ………………………………………………………………………34 圖8、P.aphrodite七個選殖體序列之對齊排列………………………………………………………………………35 圖9、P.sanderiana五個選殖體序列之對齊排列 ……………………………………………………………………36 圖10、P.stuartiana五個選殖體序列之對齊排列……………………………………………………………………37 圖11、P.schilleriana五個選殖體序列之對齊排列…………………………………………………………………38 圖12、P.equestris 五個選殖體序列之對齊排列……………………………………………………………………39 圖13、P.celebensis五個選殖體序列之對齊排列……………………………………………………………………40 圖14、P.amboinensis五個選殖體序列之對齊排列 …………………………………………………………………41 圖15、P.gigantea六個選殖體序列之對齊排列………………………………………………………………………42 圖16、P.micholitzii六個選殖體序列之對齊排列 …………………………………………………………………43 圖17、P.venosa五個選殖體序列之對齊排列…………………………………………………………………………44 圖18、P.modesta五個選殖體序列之對齊排列 ………………………………………………………………………45 圖19、P.maculata六個選殖體序列之對齊排列………………………………………………………………………46 圖20、P.mariae六個選殖體序列之對齊排列…………………………………………………………………………47 圖21、P.pallens五個選殖體序列之對齊排列 ………………………………………………………………………48 圖22、P.fasciata六個選殖體序列之對齊排列………………………………………………………………………49 圖23、P.lueddemanniana五個選殖體序列之對齊排列………………………………………………………………50 圖24、P.lued-pulchra五個選殖體序列之對齊排列…………………………………………………………………51 圖25、P.violacea五個選殖體序列之對齊排列………………………………………………………………………52 圖26、P.sumatrana五個選殖體序列之對齊排列 ……………………………………………………………………53 圖27、P.cornu-cervi五個選殖體序列之對齊排列 …………………………………………………………………54 圖28、P.mannii五個選殖體序列之對齊排列…………………………………………………………………………55 圖29、P.parishii五個選殖體序列之對齊排列………………………………………………………………………56 圖30、P.lobbii五個選殖體序列之對齊排列…………………………………………………………………………57 圖31、Doritis pulcherrima五個選殖體序列之對齊排列 …………………………………………………………58 圖32、P.equestris 五個選殖體序列之對齊排列……………………………………………………………………39 圖33、28種蝴蝶蘭與一種朵麗蘭屬植物的ITS一致性序列之對齊排列 ……………………………………………59 圖34、蝴蝶蘭屬植物之ITS-NJ樹………………………………………………………………………………………66 圖35、蝴蝶蘭屬植物ITS序列的659個最佳MP樹之一…………………………………………………………………67 圖36、蝴蝶蘭屬植物ITS一致性序列之親緣關係樹 …………………………………………………………………68 圖37、P.amabilis、P.aphrodite和P.lueddemanniana三種蝴蝶蘭分別由不同植株所得ITS序列之NJ樹………69 表次 表1、本實驗所使用的28種蝴蝶蘭原生種與一種朵麗蘭植物 ………………………………………………………70 表2、PCR反應混合液的成分……………………………………………………………………………………………72 表3、不同蝴蝶蘭植物進行PCR的黏合溫度……………………………………………………………………………73 表4、接合反應之混合液成分 …………………………………………………………………………………………74 表5、本實驗所使用的28種蝴蝶蘭及一種朵麗蘭屬植物之代號及篩選之選殖體數目 ……………………………75 表6、本實驗所使用的28種蝴蝶蘭與一種朵麗蘭屬植物之ITS1，5.8S及ITS2序列長度和GC含量之比較 ………76 表7、蘭科植物ITS區域長度及GC含量之比較…………………………………………………………………………78 表8、被子植物ITS1和ITS2長度及GC含量之比較 ……………………………………………………………………79 表9、18S-5.8S-26S rDNA之ITS序列在各種植物種內及種種間的相似度 …………………………………………81 附錄 附錄1、29種植物選殖體間的相似度 …………………………………………………………………………………83 附錄2、蝴蝶蘭屬植物ITS序列的矩陣距離……………………………………………………………………………88 | |
| dc.language.iso | zh-TW | |
| dc.title | 以rDNA之ITS序列探討蝴蝶蘭屬植物之親緣關係 | zh_TW |
| dc.title | Phylogeny of Phalaenopsis species based on ITS sequences of rDNA | en |
| dc.date.schoolyear | 91-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 93 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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