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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Chih-Chung Lin | en |
| dc.contributor.author | 林志忠 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:40Z | - |
| dc.date.available | 2021-07-01T08:12:40Z | - |
| dc.date.issued | 2002 | |
| dc.identifier.citation | 李文靜．2002．蝴蝶蘭屬植物核醣體RNA基因的選殖及實質定位．國立台灣大學植物科學研究所碩士論文． 李宜學．2000．利用螢光原位雜交探討微衛星體序列在蝴蝶蘭的實質分佈．國立台灣大學植物科學研究所碩士論文． 高玉馨．2001．以5S rDNA基因間空白區序列探討蝴蝶蘭屬植物之親緣關係．國立台灣大學植物科學研究所碩士論文． 黃建豪．1999．蝴蝶蘭兩種重複性DNA序列的分離與定性．國立台灣大學植物科學研究所碩士論文． Anamthawat-J?nsson K. and Heslop-Harrison J. S. 1994. Establishing relationships between closely related species using total genomic DNA as a probe. Meth. Mol. Biol. 50:209-225. Anamthawat-J?nsson K., Schwarzacher T., and Heslop-Harrison J. S. 1993. Behaviour of parental genomes in the hybrid Hordeum vulgare and H. bulbosum. J. Hered. 84: 78-82. Anamthawat-J?nsson K., T. Schwarzacher, A. R. Leitch, M. D. Bennett, and J. S. Heslop-Harrison. 1990. Discrimination between closely related Triticeae species using genomic DNA as a probe. Theor. Appl. Genet. 79: 721-728. Appels R., C. Driscoll, and W. J. Peacock. 1978. Heterochromatin and highly repeated DNA sequences in rye (Secale cereale). Chromosoma 70: 67-89. Arends J. C., 1970. Cytological observations on genome homology in eight interspecific hybrids of Phalaenopsis. Genetica 41: 88-100. Bennett S.T., Kenton A.Y., and Bennett M.D., 1992. Genomic in situ hybridization reveals the allopolyploid nature of Milium montianum (Gramineae). Chromosoma 101: 420-424. Cao M., Sleper D.A., Dong F., and Jiang J., 2000. Genomic in situ hybridization (GISH) reveals high chromosome pairing affinity between Lolium perenne and Festuca mairei. Genome 43: 398-403. Dressler R. L., 1993. Phylogeny and classification of the orchid family. Cambridge University Press, Cambridge. Flavell R., 1982. Sequence amplification, deletion and rearrangement: major sources of variation during species divergence. In: Dover GA, Flavell RB, eds. Genome evolution. London: Academic Press, 301-323. Fu Y. M., W. H. Chen, W. T. Tsai, Y. S. Lin, M. S. Ghyou, and Y. H. Chen. 1997. Phylogenetic studies of taxonomy and evolution among wild species of Phalaenopsis by random amplified polymorphic DNA markers. Rept. Taiwan Sugar Res. Inst. 157: 27-42. Fukui K., Shishido R., and Kinoshita T., 1997. Identification of the rice D-genome chromosomes by genomic in situ hybridization. Theor. Appl. Genet. 95: 1239-1245. Gawel N. J., and R. L. Jarret, 1991. A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Mol. Biol. Rep. 9: 262-266. Jiang J., and Gill B.S., 1993. A 'zebra' chromosome arising from multiple translocations involving nonhomologous chromosomes. Chromosoma 102: 612-617 Kao Y. Y., S. B. Chang, T. Y. Lin, C. H. Hsieh, Y. H. Chen, W. H. Chen, and C. C. Chen, 2001. Differential accumulation of hetrochromatin as a cause for karyotype variation in Phalaenopsis orchids. Ann. Bot. 87: 387-395. Kenton A., Parokonny A.S., Gleba Y.Y., and Bennett M.D., 1993. Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics. Mol. Gen. Genet. 240: 159-169. Leitch A. R., T. Schwarzacher, D. Jackson, and I. J. Leitch. 1994. In Situ Hybridization: A practical guide. BIOS Scientific Publishers, Oxford, UK. Mukai Y., and Gill B.S., 1991. Detection of barley chromatin added to wheat by genomic in situ hybridization. Genome 34: 448-452. Murray M. G., and W. F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325 Porebski S., L. G. Bailey, and B. R. Baum, 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep. 15: 8-15. Puizina J., B. Javornik, B. Bohanec, D. Schweizer, J. Maluszynska, and D. Papes, 1999. Random amplified polymorphic DNA analysis, genome size, and genomic in situ hybridization of triploid viviparous onions. Genome 42: 1208-1216. Raina S. N., and Rani V., 2001. GISH technology in plant research. Methods in Cell Science 23: 83-104 Sagawa Y., 1962. Cytological studies of the genus Phalaenopsis. Am. Orchid Soc. Bull. 31: 459-465. Sanchez-Moran E., Benavente E., and Orellana J., 1999. Simultaneous identitication of A,B,D and R genomes by genomic in situ hybridization in wheat-rye derivatives. Heredity 83: 249-252 Schwarzacher T., Leitch A.R., Bennett M.D., and Heslop-Harrison J.S., 1989. In situ localization of parental genomes in a wide hybrid. Ann. Bot. 64: 315-324. Shindo K., and H. Kamemoto, 1963. Karyotype analysis of some species of Phalaenopsis. Cytologia 28: 390-398. Shioda M., and K. Marakami-Muofushi, 1987. Selective inhibition of DNA polymerase by a polysaccharide purified from slime of Physarum polycephalum. Biochem. Biophys. Res. Commun. 146: 61-66 Snowdon R.J., K?hler W., Friedt W., and K?hler A., 1997. Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids. Theor. Appl. Genet. 95: 1320-1324. Sweet H. R., 1980. The genus Phalaenopsis. Day Printing Corp., Pomon, California. Woodard. J. W., 1951. Some chromosome numbers in Phalaenopsis. Am. Orchid Soc. Bull. 20: 356-358. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75321 | - |
| dc.description.abstract | 八種蝴蝶蘭雜交種進行基因組螢光原位雜交的結果，四種雜交種Phalaenopsis mannii x P. stuartiana、P. violacea x P. equestris、P. stuartiana x P. amboinensis、P. equestris x Doritis pulcherrima，不使用覆蓋DNA即可清楚區分兩親本的染色體，顯示大小染色體所含重複性DNA的種類及數量不同，且小染色體中節處含特有的重複性序列，因此具大染色體的蝴蝶蘭與具小染色體的蝴蝶蘭同源性較低，親緣關係較疏遠。雜交種P. mannii x P. lueddemanniana使用50倍覆蓋DNA時才能區分兩親本染色體，兩者同源性較高，親緣關係較接近。雜交種P. sanderiana x P. aphrodite及P. mannii x P. violacea，在加或不加覆蓋DNA的情況下，均無法區分兩親本的染色體，顯示P. sanderiana與P. aphrodite間以及P. mannii與P. violacea間其重複性DNA序列有極高之同源性。雜交種P. violacea x D．pulcherrima不使用覆蓋DNA即可辨別兩親本染色體，添加50倍覆蓋DNA，更可清楚區分，顯示此兩種蝴蝶蘭之重複性DNA序列稍有差異。 | zh_TW |
| dc.description.abstract | Genomic in situ hybridization was used to investigate the genome organization and their relationships of eight Phalaenopsis hybrids. The result revealed that (1) In hybrids P. mannii x P. stuartiana, P. violacea x P. equestris, P. amboinensis x P. stuartiana, P. equestris x D. pulcherrima, the parental genomes could be distinguished without blocking DNA as result of differences in kind and amount of repetitive sequences. Species with small chromosomes had their own specific sequences at the centromeric regions. Thus it is considered that these species were remotely related. (2) Except 50-fold blocking DNA the parental chromosomes of hybrid P. mannii x P. lueddemanniana could not be identify. It is suggested that these two species were closely related. (3) In hybrids P. mannii x P. violacea, P. aphrodite x P. sanderiana, the parental chromosomes could not be discriminated even with 100-fold blocking DNA. It is indicated that species with small genomes or with large genomes contained high homology of repetitive sequences respectively. (4) In hybrid P. violacea x D. pulcherrima, the two genomes could be differentiated with or without blocking DNA. The relationships between these two species seemed distinctly. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2002 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………i 英文摘要……………………………………………………ii 壹、前言……………………………………………………1 貳、材料與方法 一、植物材料……………………………………………………7 二、蝴蝶蘭總DNA的抽取……………………………………………………7 三、探針的標定……………………………………………………8 四、覆蓋DNA的製備……………………………………………………8 五、基因組螢光原位雜交……………………………………………………9 參、結果……………………………………………………14 肆、討論……………………………………………………25 伍、參考文獻……………………………………………………29 | |
| dc.language.iso | zh-TW | |
| dc.title | 以基因組螢光原位雜交探討蝴蝶蘭雜交種之基因組組成 | zh_TW |
| dc.title | Investigation of genome organization in Phalaenopsis hybrids by GISH | en |
| dc.date.schoolyear | 90-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 32 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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