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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Yi-Hsing Lin | en |
| dc.contributor.author | 林薏欣 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:31Z | - |
| dc.date.available | 2021-07-01T08:12:31Z | - |
| dc.date.issued | 2001 | |
| dc.identifier.citation | 廖一久。1969。蝦類養殖試驗。中國水產學會五十八年年會專題演講。
楊仕音。2000。一種新的100kDa蝦白點症病毒蛋白質之研究。國立台灣大學動物學研究所碩士論文。 劉宛菁。2000。蝦白點症病毒蛋白激?基因之研究。國立台灣大學碩士論文。 蔡孟峰。2000。蝦白點症病毒之研究：病原性分析以及tk-tmk和rr基因特性之研究。國立台灣大學動物學研究所博士論文。 陳歷歷。2001。白點症病毒vp35及np1基因之表現及其性質鑑定。國立台灣大學動物學研究所博士論文。 Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., and Lipman D. J. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402. Bairoch A., Bucher P., and Hofmann K. (1997). The PROSITE database, its status in 1997. Nucleic Acids Res. 25, 217-221. Beaud G, and Beaud R. (1997). Preferential virosomal location of underphosphorylated H5R protein synthesized in vaccinia virus-infected cells. J. Gen. Virol. 78, 3297-3302. Blissard G. W., and Rohrmann G. F. (1990). Baculovirus diversity and molecular biology. Annu. Rev. Entomol. 35, 127-155. Boesen C. C., Motyka S. A., Patamawenu A., and Sun P. D. (2000). Development of a recombinant bacterial expression system for the active form of a human transforming growth factor beta type Ⅱ receptor ligand binding domain. Protein Expr. Purif. 20, 98-104. Chang Y. S., Lo C. F., Wang Y. C., and Kou G. H. (1996). Identification of white spot syndrome associated baculovirus (WSSV) target organs in shrimp, Penaeus monodon, by in situ hybridization. Dis. Aquat. Org. 27, 131-139. Chang Y. S., Lo C. F., Peng S. E., Liu K. F., Wang C. H., and Kou G. H. (2002). White spot syndrome virus (WSSV) PCR positive Artemia cysts yield PCR negative naupii that fail to transmit WSSV to shrimp post larvae. Dis. Aquat. Org. 49, 1-10. Chen J., Marechal V., and Levine A. J. (1993). Mapping of the p53 and mdm-2 interaction domains. Mol. Cell Biol. 13, 4107-4114. Chen S. N., Chang P. S., and Kou G. H. (1989). Observation on pathogenicity and epizootiology of Penaeus monodon baculovirus (MBV) in cultured shrimp in Taiwan. Fish Pathol. 24, 189-195. Chen L. L., Lo C. F., Chiu Y. L., Chang C. F., and Kou G. H. (2000). Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata. Dis. Aquat. Org. 40, 157-161. Chen L. L., Leu J. H., Huang C. J., Chou C. M., Chen S. M., Wang C. H., Lo C. F. and Kou G. H. (2002a). Identification of a nucleocapsid protein (VP35) gene of shrimp white spot syndrome virus and characterization of the motif important for targeting VP35 to the nuclei of transfected insect cells. Virology 293, 44-53. Chen L. L., Wang H. C., Huang C. J., Peng S. E., Chen Y. G., Lin S. J., Chen W. Y., Dai C. F., Yu H. T., Wang C. H., Lo C. F. and Kou G. H. (2002b). Transcription analysis of the DNA polymerase gene of shrimp white spot syndrome virus (WSSV). Viroilogy (in press). Chou H. Y., Huang C. Y., Wang C. H., Kou G. H., and Lo C. F. (1995). Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis. Aquat. Org. 23, 165-173. Couch J. A. (1974). Free and occluded virus, similar to Baculovirus, in hepatopancreas of pink shrimp. Nature 247, 229-231. Crook N. E., and Jarrett P. (1991). Viral and bacterial pathogens of insects. Soc. Appl. Bacteriol Symp. Ser. 20, 91S-96S. Dahl R., and Kates J. R. (1970). Intracellular structures containing vaccinia DNA: isolation and characterization. Virology 42, 453-462. Domi A., and Beaud G. (2000). The punctate sites of accumulation of vaccinia virus early proteins are precursors of sites of viral DNA synthesis. J. Gen. Virol. 81, 1231-1235. Du X., and Thiem S. M. (1997). Responses of insect cells to baculovirus infection: protein synthesis shutdown and apoptosis. J. Virol. 71, 7866-7872. Dutta S, Daugherty JR, Ware LA, Lanar DE, and Ockenhouse CF. (2000). Expression, purification and characterization of a functional region of the Plasmodium vivax Duffy binding protein. Mol. Biochem. Parasitol. 109, 179-184. Forhman M. A., Dush M. K., and Martin G. R. (1988). Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. Franken K. L., Hiemstra H. S., van Meijgaarden K. E., Subronto Y., den Hartigh J., Ottenhoff T. H., and Drijfhout J. W. (2000). Purification of his-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent. Protein Expr. Purif. 18, 95-99. Fuchs L. Y., Woods M. S., and Weaver, R. F. (1983). Viral transcription during Autographa californica nuclear polyhedrosis virus infection: a novel RNA polymerase induced in infected Spodoptera frugiperda cells. J. Virol. 43, 641-646. Fulk M., and Main K. L. (1992). Introduction. In: W. Fulks and K. L. Main (Eds.). Proceedings of a workshop of the international conference on diseases of cultured penaeid shrimp in Asia and the United States. Honolulu, Hawaii, U.S.A., p3-33. Grula M. A., Buller P. L., and Weaver R. F. (1981). -Amanitin-resistant viral RNA synthesis in nuclei isolated from nuclear polyhedrosis virus-infected Heliothis zea larvae and Spodoptera frugiperda cells. J. Virol. 38, 916-921. Hara-Kuge S., Ohkura T., Seko A., and Yamashita K. (1999). Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1-->2 mannosyl residues in MDCK cells. Glycobiology 9, 833-839. Hara-Kuge S., Ohkura T., Ideo H., Shimada O., Atsumi S., and Yamashita K. (2002). Involvement of VIP36 in intracellular transport and secretion of glycoproteins in polarized Madin-Darby canine kidney (MDCK) cells. J. Biol. Chem. 3, 16332-16339. Hopp T. P., and Woods K. R. (1983). A computer program for predicting protein antigenic determinants. Mol. Immunol. 20, 483-489. Hooft van Iddekinge B. J. L., Smith G. E., and Summers M. D. (1983). Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology. 131, 561-565. Huh N. E., and Weaver R. F. (1990). Categorizing some early and late transcripts directed by the Autographa californica nuclear polyhedrosis virus. J. Gen. Virol. 71, 2195-2200. Klein P., Somorjai R. L., and Lau P. C. (1988). Distinctive properties of signal sequences from bacterial lipoproteins. Protein Eng. 2, 15-20. Kozak M. (1989). The scanning model for translation: an update. J. Cell Biol. 108, 229-241. Kozak M. (1991). An analysis of vertebrate mRNA sequences: intimations of translational control. J. Cell Biol. 115, 887-903. Kozak M. (1995). Adherence to the first-AUG rule when a second AUG codon follows closely upon the first. Proc. Natl. Acad. Sci. USA 92, 2662-2666. Kuzio J., Rohel D. Z., Curry C. J., Krebs A., Carstens E. H., and Faulkner P. (1984). Nucleotide sequence of the p10 polypeptide gene of Autographa californica nuclear polyhedrosis virus. Virology 139, 414-418. Liao I. C. (1989a). Penaeus monodon culture in Taiwan: Through two decades of growth. Int. J. Aquacult. Fish. Technol. 1, 16-24. Liao I. C. (1989b). Taiwanese shrimp culture: A molting industry. In: K. Chauvun, P. Menesses, W. Chauvin and A. Cuccia, eds., Proc. Shrimp World TV (The Fourth Shrimp World Marketing Conference), Shrimp World Incorporated, New Orleans, Louisiana, U.S.A., p55-83. Liu W. J., Yu H. T., Peng S. E., Chang Y. S., Pien H. W., Lin C. J., Huang C. J., Tsai M. F., Wang C. H., Lin J. Y., Lo C. F., and Kou G. H. (2001). Cloning, characterization, and phylogenetic analysis of a shrimp white spot syndrome virus gene that encodes a protein kinase. Virology 289, 362-377. Lo C. F., Ho C. H., Peng S. E., Chen C. H., Hsu H. C., Chiu Y. L., Chen Y. T., Chang C. F., Liu K. F., Su M. S., Wang C. H., and Kou G. H. (1996a). Infection of white spot syndrome associated virus (WSBV) in cultured and wild-caught shrimps, crabs and other arthropods. Dis. Aquat. Org. 27, 215-225. Lo C. F., Leu J. H., Ho C. H., Chen C. H., Peng S. E., Chen Y. T., Chou C. M., Yeh P. Y., Huang C. J., Chou H. Y., Wang C. H., and Kou G. H. (1996b). Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Dis. Aquat. Org. 25, 133-141. Lo C. F., Ho C. H., Chen C. H., Liu K. F., Chiu Y. L., Yeh P. Y., Peng S. E., Hsu H. C., Liu H. C., Chang C. F., Su M. S., Wang C. H., and Kou G. H. (1997). Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Dis. Aquat. Org. 30, 53-72. Lo C. F., Hsu H. C., Tsai M. F., Ho C. H., Peng S. E., Kou G. H., and Lightner D. V. (1999). Specific genomic DNA fragment analysis of different geographical clinical samples of shrimp white spot syndrome virus. Dis. Aquat. Org. 35, 175-185. Luckow A. V., and Summers M. D. (1989). Trends in the development of baculovirus expression vector. Bio/Technology 6, 47-55. Lupas A. (1996). Coiled coils: new structures and new functions. Trends Biochem. Sci. 21, 375-382. Lupas A. (1997). Predicting coiled-coil regions in proteins. Curr. Opin. Struct. Biol. 7, 388-393. Makkerh J. P. S., Dingwall C., and Laskey R. A. (1996). Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids. Curr. Biol. 6, 1025-1027. Marston F. A. (1986). The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 15, 1-12. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., and Summer M. D. (1995). Classification and nomenclature of viruses. Arch. Virol., suppl. 10, 1-586. Pearson W. R., and Lipman D. J. (1988). Improved tools for biological sequence analysis. Proc. Natl. Acad. Sci. USA 85, 2444-2448. Possee R. D., and Howard S. C. (1987). Analysis of the polyhedrin gene promoter of the Autographa calfornica nuclear polyhedrosis virus. Nucl. Acids Res. 15, 10233-10248. Rohrmann G. F. (1986). Polyhedrin structure. J. Gen. Virol. 73, 749-761. Rosenquist G. L., and Nicholas H. B. Jr. (1993). Analysis of sequence requirements for protein tyrosine sulfation. Protein Sci. 2, 215-222. Sambrook J., Fritsch E. F., and Maniatis T. (1989). Molecular cloning: A laboratory manual, 2nd edition. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. Shmulevitz M, and Duncan R. (2000). A new class of fusion-associated small transmembrane (FAST) proteins encoded by the non-enveloped fusogenic reoviruses. EMBO J. 19, 902-912. Thiem S., and Miller L. K. (1989). Identification, sequence, and transcriptional mapping of the major capsid protein gene of the baculovirus Autographa californica nuclear polyhedrosis virus. J. Virol. 63, 2008-2018. Tolonen N., Doglio L., Schleich S., and Krijnse Locker J. (2001). Vaccinia virus DNA replication occurs in endoplasmic reticulum-enclosed cytoplasmic mini-nuclei. Mol. Biol. Cell 12, 2031-2046. Tsai M. F., Lo C. F., van Hulten M. C. W., Tzeng H. F., Chou C. M., Huang C. J., Wang C. H., Lin J. Y, Vlak J. M., and Kou G. H. (2000a). Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus. Virology, 277, 92-99. Tsai M. F., Yu H. T., Tzeng H. F., Leu J. H., Chou C. M., Huang C. J., Wang C. H., Lin J. Y., Kou G. H., and Lo C. F. (2000b). Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology, 277, 100-110. van Hulten M. C., W., Tsai M. F., Schipper C. A., Lo C. F., Kou G. H., and Vlak J. M. (2000a). Analysis of a genomic segment of white spot syndrome virus of shrimp containing ribonucleotide reductase genes and repeat regions. J. Gen. Virol. 81, 307-316. van Hulten M. C., Westenberg M., Goodall S. D., and Vlak J. M. (2000b). Identification of two major virion protein genes of white spot syndrome virus of shrimp. Virology 266, 227-236. van Hulten M. C., Witteveldt J., Snippe M., and Vlak J. M. (2001a). White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology 285, 228-233. van Hulten M. C., Witteveldt J., Peters S., Kloosterboer N., Tarchini R., Fiers M., Sandbrink H., Lankhorst R. K., and Vlak J. M. (2001b). The white spot syndrome virus DNA genome sequence. Virology 286, 7-22. van Hulten M. C., Reijns M., Vermeesch A. M., Zandbergen F., and Vlak J. M. (2002). Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins. J. Gen. Virol. 83, 257-265 Wang C. H., Lo C. F., Leu J. H., Chou C. M., Yeh P. Y., Chou H. Y., Tung M. E., Chang C. F., Su M. S., and Kou G. H. (1995). Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Dis. Aquat. Org. 23, 239-242. Wilson M. E., Mainprize T. H., Friesen P. D., and Miller L. K. (1987). Location, transcription, and sequence of baculovirus gene encoding a small arginine-rich polypeptide. J. Virol. 61, 661-666. Wychowski C., Benichou D., and Girard M. (1986). A domain of SV40 capsid polypeptide VP1 that specifies migration into the cell nucleus. EMBO J. 5, 2569-2576. Wychowski C., Benichou D., and Girard M. (1987). The intranuclear localization of simian virus 40 polypeptides Vp2 and Vp3 depends on a specific amino acid sequence. J. Virol. 61, 3862-3869. Yang F., Wang W., Chen R. Z., and Xu X. (1997). A simple and efficient method for purification of prawn baculovirus DNA. J. Virol. Methods 67, 1-4. Yang F., He J., Lin X., Li Q., Pan D., Zhang X., and Xu X. (2001). Complete genome sequence of the shrimp white spot bacilliform virus. J. Virol. 75, 11811-11820. Zhang X., Xu X., and Hew C. L. (2001). The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus. Virus. Res. 79, 137-144. Zhang X., Huang C., Xu X., and Hew C. L. (2002a). Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus. J. Gen. Virol. 83, 471-477. Zhang X., Huang C., Xu X., and Hew C. L. (2002b). Identification and localization of a prawn white spot syndrome virus gene that encodes an envelope protein. J. Gen. Virol. 83, 1069-1074. Zhang X., Huang C., Xu X., and Hew C. L. (2002b). Identification and localization of a prawn white spot syndrome virus gene that encodes an envelope protein. J. Gen. Virol. 83, 1069-1074. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75285 | - |
| dc.description.abstract | 白點症病毒(white spot syndrome virus, WSSV)台灣分離株Taiwanisolate,AF440570)所預測的開放譯讀區WSSV067經由研究，命名為p150基因，並將此基因進行分析比對。p150基因全長3,906 bp，可轉錄出1,301個胺基酸，經由電腦分析P150蛋白是屬於酸性蛋白，預測的分子量大小為143.9 kDa，其蛋白胺端(N-terminus)具有兩個glutamic acid-rich domain，羧端(C-terminus)具有三個穿越膜區域(transmembrane region)。p150基因的轉錄本(transcript)大小約為4.3 kb，此基因於白點症病毒感染後12小時的蝦體才能被檢測出，是屬於晚期表現基因，其加聚腺嘌呤位置(Poly A addition site)在加聚腺嘌呤訊號下游第15個核?酸處，與目前已知的白點症病毒基因rr1、DNApol以及預測的開放譯讀區WSSV202、WSSV217比較，在這個區域內可以找到一個保守性序列(T/A)ACATGTA(T/A)A(T/A)，其中包含了一段可折疊相對應的序列ACATGT。經由分析白點症病毒基因p150在轉錄、轉譯方面的序列特性，推測其基因的表現可能是利用病毒本身所特有的機制調控。另外經由試管內轉錄轉譯實驗，表現p150全長基因，可得分子量約150 kDa的蛋白產物。以昆蟲細胞株Sf9進行轉染作用，可觀察到P150蛋白存在於細胞質中，並可能與某種胞器結合，經由共軛焦顯微鏡觀察，確認與溶小體及粒線體無關，但可能具有與細胞內質網結合的特性，推測白點症病毒p150基因可能和病毒顆粒的形成有關。 | zh_TW |
| dc.description.abstract | One of the white spot syndrome virus (WSSV) genes, p150, that encodes an acidic protein was identified and sequenced from the predicted open reading frame, WSSV067, from the Taiwan isolate. The p150 gene that contains 3,906 nucleotides potentially encodes a protein of 1,301 amino acids with two glutamic acid-rich domains in the N-terminus and three transmembrane regions in the C-terminus. The predicted molecular weight of P150 is 143.9 kDa. The transcript of p150, which was detected at 12 hr post-infection and reached a maximal level at 18 hr, belonged to a late gene of WSSV. The mRNA size of p150 gene is about 4.3 kb, and the poly A addition site is 15 bp downstream of the poly A signal. When compare the region between poly A signal and poly A addition site of known WSSV genes of p150, rr1, DNApo1 and the predicted open reading frame WSSV202 and WSSV217, a consensus sequence was found, (T/A)ACATATA(T/A)A(T/A). The product of in vitro transcription and translation of p150 gene was detected as a 150 kDa protein. Immunofluorescent staining of P150 protein in insect cell line showed that the P150 localized in cytoplasm, and might bind to some organelles. P150 did not associate with lysosome and mitochondria according to the observation under Confocal microscope, but might be colocalized with endoplasmic reticulum (ER) under fluorescence microscope. The P150 of WSSV may participate in virus assembly. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2001 | en |
| dc.description.tableofcontents | 緒論……………………………………………………………………………………………………………………………1 前言……………………………………………………………………………………………………………………………5 材料與方法 一、核?酸序列及胺基酸序列分析………………………………………………………………………………………7 二、檢體RNA的萃取 ………………………………………………………………………………………………………7 三、北方轉印雜合法(Northern blot hybridization) ………………………………………………………………8 四、快速增殖cDNA末端(Rapid amplification of cDNA ends, RACE)………………………………………………12 五、重組質體DNA的構築 …………………………………………………………………………………………………14 六、以桿狀病毒表現系統生產重組蛋白…………………………………………………………………………………18 七、試管內轉錄和轉譯反應(in vitro transcription and translation)…………………………………………24 八、蝦體全蛋白的粗萃取…………………………………………………………………………………………………25 九、西方轉印雜合法(Western blot hybridization)…………………………………………………………………25 十、螢光重組蛋白表現位置………………………………………………………………………………………………26 結果 一、白點症病毒p150基因核?酸序列及胺基酸序列分析………………………………………………………………31 二、白點症病毒p150基因轉錄特性分析…………………………………………………………………………………33 三、P150部分蛋白表現及純化方式………………………………………………………………………………………33 四、P150蛋白表現分析……………………………………………………………………………………………………34 五、P150蛋白於細胞中之定位……………………………………………………………………………………………35 討論……………………………………………………………………………………………………………………………37 參考文獻………………………………………………………………………………………………………………………44 圖表……………………………………………………………………………………………………………………………56 | |
| dc.language.iso | zh-TW | |
| dc.title | 白點症病毒晚期基因p150表現之研究 | zh_TW |
| dc.title | Expression analysis of white spot syndrome virus (WSSV) late gene p150 | en |
| dc.date.schoolyear | 90-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 67 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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