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DC 欄位 | 值 | 語言 |
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dc.contributor.author | Chiu-Ju Huang | en |
dc.contributor.author | 黃秋茹 | zh_TW |
dc.date.accessioned | 2021-07-01T08:12:25Z | - |
dc.date.available | 2021-07-01T08:12:25Z | - |
dc.date.issued | 2001 | |
dc.identifier.citation | 周紀源,國立臺灣大學漁業科學研究所碩士論文:不同外來基因的組成對基因轉殖魚的嵌入性及其表現的影響,八十八年 林哲揚,國立臺灣大學漁業科學研究所碩士論文:吳郭魚c-ski基因的選殖、定序及比較,八十五年 鍾至青,國立臺灣大學漁業科學研究所碩士論文:應用水母綠螢光蛋白研究基因在胚胎發育早期的表現動態,八十六年 Akiyoshi, S., Inoue, H., Hanai, J., Kusanagi, K., Nemto, N., Miyazono, K. and Kawabata, M. (1999) c-Ski Acts as a transcriptional co-repressor in transforming growth factor-13 signaling through interaction with Smads. J. Biol. Chem.274: 35269-35277. Barkas, A. E., Brodeur, D. and Stavenzer, E. (1986) Polyprotein containing a domain encodes by the v-ski oncogene. Virology 151: 13 1-138 Berk, M., Desai, S.Y., Heyman, H.C. and Colmenares, C. (1997) Mice lacking the ski proto-oncogene have defects in neurulation, craniofacial patterning, and skeketal muscle development. Genes Dev.11: 2029-2039. Bishop, M. J. (1994) Guide to Human Genome Computing. Editor. Academic Press, San Diego. Bowen, R. A., Reed, M. L., Schnieke, A., Seidel, G. E. JR., Stacey, A., Thomas, W. K. and Kajikawa, O. (1994) Transgenic castle resulting from biopsied embryos: expression of c-ski in a transgenic calf. Biol. Reprod. 50:664-668. Chou, P. Y. and Fasman, G. D. (1978) Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47: 45-148. Colmenares, C and Stavnezer, E. (1989) The ski oncogene induces muscle differentiation in quail embryo cells. Cell 59: 293-303. Colmenares, C., Sutrave, P., Hughes, S., Stavnezer, E. (1991) Activation of the c-ski oncogene by overexpression. J Virol 65: 4929-4935. Dahl, R., Kinslinger, M., Beug, H. and Hayman, M. (1998) Transformation of hematopoietic cells by Ski oncoprotein involves repression of retinoic acid receptor signling. Proc. Natl. Acad. USA 95:11187-11192. Eisenberg, D., Sweet, R. H. and Terwilliger, T. C. (1984) The hydrophobic moment detects periodicity in protein hydrophobicity. Proc. Natl. Acad. Sci. U.S.A. 81: 140-144. Engert, J. C., Servaes, S., Sutrave, P., Hughes, S. H. and Rosenthal, N. (1995) Activation of muscle-specific enhancer by the ski protooncogene. Nucleic Acids Res. 23: 2988-2994. Gamier, J., Osguthorpe, D. J. and Robson, B. (1978). Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120: 97-120. Grimes, H. L., Szente, B. E. and Goodenow, M. M. (1992) c-ski cDNA are encoded by eight exons, six of which are closely linked within the chicken genom. Nucleic Acids Res. 20: 1511-1516. Grimes, H. L., Ambrose, M. R. and Goodenow, M. M. (1993) c-ski transcripts with and without exon 2 are expressed in skeletal muscle and throughout chick embryogenesis. Oncogene 8: 2863-2868. Higgins, D.G. and Sharp, P. M. (1988) CLUSTAL: A package for performing multiple sequence alignment on microcomputer. Gene 73:237-244. Heyman, H. C. and Stavnezer, E. (1994) Caroxyl-terminal region of the ski oncoprotein mediates homodimerization as well as heterodimerization with the relate protein SnoN. J. Biol. Chem. 269: 26996-27003 Ichikawa, K., Nagase, T., Ishii, S. and Mimura, N. (1997) Trans-regulation of myogenin promoter/enhancer activity by c-ski during skeletal- muscle differentiation: the c-terminal of the c-Ski protein is essential for transcriptional regulatory activity in myotubes. Biochem. J. 328:607-613. Kaufman, C. D., Maruines-Rodrigues, G. and Hackett P. B. JR. (2000) Ectopic expression of c-ski disrupts gastrulation and neural patterning in zebrafish. Mech. Dev. 95: 147-162. Kozak, M. (1978) How do eucaryotic ribosomes select initiation regions in messenger RNA. Cell 15: 1109-1123. Lana, D. P., Leferovich, j. M., Kelly, A. M. and Hughes, S. H. (1996) Selective expression of a ski transgene affects Jib fast muscles and skeletal structure. Dev. Dyn. 205:13-23. Larsen, J., Meyer, S., Steinlein, P., Beug, H. and Hayman, M. J. (1993) Transformation of chicken bone marrow cells by the v-ski oncogene. Oncogene 8: 3221-3228. Larsen, J., Beug, H. and Hayman, M. J. (1993) The v-ski oncogene cooperates with the v-sea oncogene in erythroid transformation by blocking erythroid differentiation. Oncogene 7: 1903-1911. Leferovich, J. M., Lana, D. P., Sutrave, P., Hughes, S. H. and Kelly, A. M. (1995) Regulation of c-ski transgene expression in developing and mature mice. J. Neuri. Sci. 15: 596-603. Li, Y., Turk, C. M., Teumer, J. K. and Stavnezer, E. (1986) Unique sequence, ski, in Sloan-Kettering avian retroviruses with properties of a new cell-derived oncogene. J. Virol. 57: 1065-1072. Liu, Y. J., Zhu, Z. Y., Roberg, K., Faras, A., Guise, K., Kapusinski, R. and Hackett, P. B. (1990) Isolation and characterization of beta-actin gene of carp (Cyprinus carpio). DNA Seq. 1: 125-136. Ludolph, D. C., Neff, A.W., Parker, M. A., Mescher, A. L., Smith, R.C. and Malacinski, G. M. (1995) Cloning and expression of the axolotl proto-oncogene ski. Biochim Biophys Acta 1260: 102-104. Lyons, G. E., Micales, B. K., Herr, M. J. Horrigan, S. K., Namciu, S., Shardy, D. and Stavnezer, E. (1994) Proto-oncogene c-ski is expressed in proliferating and postmitotic neuronal populations. Dev. Dyn. 201:354-365. Nagase, T., Mizuguchi, G., Nomura, N., Ishizaki, R., Ueno, Y. and Ishii. S. (1990) Requirement of protein co-factor for the DNA-binding function of human ski proto-oncogene product. Nucleic Acids Res. 8: 337-343. Nagase, T., Nomura, N. and Ishii, S. (1993) Complex formation between proteins encoded by the ski gene family. J. Biol. Chem. 268: 13710-13716. Namciu, S., Lieberman, M. A. and Stavnezer, E. (1994) Induction of the cski proto-oncogene by phorbol ester correlates with induction of megakaryocyte differentiation. Oncogene 9: 1407-1416. Namciu, S., Lyons, G. E., Micales, B. K., Heyman, H. C., Colmenares, C. and Stavnezer, E. (1995) Enhanced expression of mouse c-ski accompanies terminal skeletal muscle differentiation in vivo and in vitro. Dev. Dyn. 204: 291-300. Nicol, R., and Stavnezer, E. (1998) Transcriptional repression by v-Ski and c-Ski mediated by a specific DNA binding site. J. Biol. Chem. 273:3588-3597. Nomura, T., Khan, M. M., Kaul, S. C., Dong, H. D., Wadhwa, R., Colmenares, C., Kohno, I. and Ishii, S. (1999) Ski is a compoenet of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor. Genes Dev. 13: 412-423. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) In: Nolan, C. editor. Molecular Cloning. Cold Spring Harbor Press, New York. p9.1-9.58. Sanger, F., Nicklen, S., Coulson, A. R. (1977) DNA sequence with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74: 5463-5467. Sleeman, J. P. and Laskey, R. A. (1993) Xenopus c-ski contains a novel coiled-coil protein domain, and is maternally expressed during development. Oncogene 8: 67-77. Stavnezer, E., Gerhard, D. S., Binari, R. C. and Batzas, I. (1981) Generation of transforming viruses in cultures of chicken fibroblast infected with avian leukosis virus. J. Virol. 39: 920-934. Stavnezer, E., Barkas, A.E., Brennan, L. A., Brodeur, D. and Li, Y. (1986) Transforming Sloan-Kettering viruses generated from the cloned v-ski oncogene by in vitro and in vivo recombinations. J. Virol. 57: 1073-1083. Stavnezer, E., Brodeur, D. and Brennan, L. A. (1989) The v-ski oncogene encodes a truncated set of c-ski coding exons with limited sequebce and structural relatedness to v-myc. Mol. Cell. Biol. 9: 4038-4045. Sutrave, P. and Hughes, S. H. (1989) Isolation and characterization of three distinct cDNAs for the chicken c-ski gene. Mol. Cell Biol. 9: 4046-4051. Sturave, P., Copeland, T. D., Showlter, S. D. and Hughes, S. H. (1990) Characterization of chicken c-ski oncogene products expressed by retrovirus vectors. J. Cell. Biol. 10: 3137-3144. Sutrave, P., Kelly, A. M. and Hughes, S. H. (1990) Ski can cause selective growth of skeletal muscle in transgenic mice. Genes Dev. 4:1462-1472. Sutrave, P. and Hughes, S. H. (1991) The ski oncogene. Oncogene 6: 353-356. Sutrave, P., Leferovich, J. M., Kelly, A. M. and Hughes, S. H. (2000) the induction of skeletal muscle hypertrophy by a ski transgene is promoter-dependent. Gene 241: 107-116. Tarapore, P., Richmond, C., Zheng, G., Cohen, S. B., Kelder, B., Kopchick, J., Kruse, U., Sippel, A. E., Colmenares, C. and Stavnezer, E., (1997) DNA binding and transcriptional activation by the Ski oncoprotein mediated by interaction with NFI. Nucleic Acids Res. 25: 3895-3903. Tokitou, F., Nomura, T., Khan, M. M., Kual, S. C., Wadhwa, R., Yasukawa, T., Kohno, I. and Ishii, S. (1999) Viral ski inhibits retinoblastoma protein (Rb)-mediated transcriptional repression in a dominant negative fashion. J. Biol. Chem. 19: 4485-4488. Zheng, G., Blumenthal, K. M., Ji, Y., Shardy, D. L., Cohen, S. B., and Stavnezer, E. (1997) High affinity dimerization by Ski involves parallel pairing of a novel bipartite x-helical domain. J. Biol. Chem.272: 31855-31864. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75260 | - |
dc.description.abstract | 本論文對於兩型吳郭魚c-ski基因(tski1, tski2)的研究內容包括有解析c-ski基因的序列及結構;分析c-ski mRNA在吳郭魚不同組織及發育過程中的表現,還有初步分析這兩型基因?動子的性質三部份。 在基因結構的部份;由16公分長的吳郭魚所萃取出的mRNA中定義出兩型的c-ski cDNA,命名為tski1、tski2,分別可轉譯出687及714個氨基酸序列,和在雞、人、爪蟾所發表過的序列各有60%、57%、57% (tski1)和百分之67%、63%、61% (tski2)的相似度。從genomic DNA library選殖出的tski1、tski2基因在染色體中至少包含16kb與20kb的genomic DNA序列,其中分別具有3kb及2kb的?動子區域,1.5 kb及1.3 kb的exon1,並有大於10 kb及2 kb的序列介於exon1、exon2之間,之後exon2到exon7兩種型的tski都一樣是約2kb大小的genomic DNA,而最後的exon7,前段是可轉譯區域(coding region)後接著0.4kb (tski1)、0.8 kb(tski2)的非轉譯區。 在基因表現的部份;對吳郭魚成魚的各種組織進行mRNA表現的觀察發現tski1和tski2廣泛的表現在各組織,其中腦表現最多,骨骼肌則表現最少。而tski1在卵巢及成熟卵細胞的表現量較tski2高出許多;tski2在精巢的表現量較tski1高出許多。發育早期過程中,不同時期tski1的表現是母體遺傳(maternally-inherited),從未授精的卵開始到四公分小魚,皆有表現。tski2則是從胚胎到四公分小魚,皆有表現。而tski1和tski2表現程度的比值有逐漸下降的趨勢。在?動子分析的部份;將tski1現有的3 kb起動子區域與tski2現有的2kb起動子區域進行EGFP的斑馬魚顯微注射,tski1起動子區域在斑馬魚授精後58小時可清楚觀察到脊索(notochord)、骨骼肌、心肌、尾鰭、眼部細胞的螢光;而相同時期所觀察到的tski2起動子所驅動的表現位置大致與tski1相同但螢光表現是零星分佈且微弱很多。 | zh_TW |
dc.description.abstract | Two classes of tilapia c-ski cDNA (Accession number: AJ012011, AJ012012), designated as tski1 and tski2, respectively encoded a 687 and a 714 AA protein and shared a 57% AA identity. Comparison with the Ski proteins of chickens, humans and Xenopus, tilapia TSki polypeptides shared a 60, 57 and 57% (TSki1) and 67, 63 and 61% (TSki2) AA identity, respectively. The tski1 and tski2 gene extend a minimum of 16 kb and 20kb in the tilapia genome. The exon 1 comprising 1.5 kb (tski1) and 1.3kb (tski2) of cDNA sequence have been mapped with screening tilopia genomic DNA library. Then exons 1 and 2 are separated by more than 10 kb (tski1) and 2kb (tski2) of genomic sequence. Using SSP-PCR (single specific primer-polymerase chain reaction) and PCR with genomic DNA templates, it is clear that tski1 and tski2 cDNA are encoded seven exons. In this two genes, the exons 2 through 7 are closely linked within 2 kb in the tilopia genome. The long 3’ untranslated region is contiguous in the genome with the distal portion of the open reading frame such that the exon 7 is composed of both coding and noncoding sequence. The most and the least abundant c-ski mRNAs are located in the brain and the skeletal muscle, respectively. Both tski1 and tski2 were widely expressed in the adult tissues examined, but tski2 transcripts were at higher levels except in the ovary and oocytes: tski1 transcripts were predominant in the ovary, whereas tski2 transcripts were predominant in the testes. In the oocytes, the tski1 mRNA was a maternally-inherited stockpile that subsequently was degraded, so that the expression ratio of tski1 to tski2 transcripts declined gradually as the fish developed from oocyte to 4-cm fry. The notochord, eye, muscle fiber, tail fin and heart transient expression pattern showed that the 3 kb tski1 promoter promotes tski1 gene wildly in zebrafish. As to the pT2EGFP, had the same EGFP expression pattern with pT1EGFP but weak and mosaic extremely. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2001 | en |
dc.description.tableofcontents | 摘要. . . . . . . . . . . . . . . . . . . . . . 1 Abstract . . . . . . . . . . . . . . . . . . . . . . . 3 緒論. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1. c-ski基因的起源. . . . . . . . . . . . . . . . . . . . 5 2. c-ski基因的序列. . . . . . . . . . . . . . . . . . 6 3. c-Ski蛋白質的性質. . . . . . . . . . . . . . . . . . . . . 8 4. c-ski基因的表現. . . . . . . . . . . . . . . . . 9 5. c-ski基因的功能. . . . . . . . . . . . . . . . . . . . . . 10 6. c-ski基因的轉殖動物. . . . . . . . . . . . . . . . . 11 7. c-ski基因所參與的作用機制. . . . . . . . . . . . . . . . . . . . 13 8. 本實驗的簡介. . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 材料與方法. . . . . . . . . . . . . . . . . . . . . . . . . . 16 結果. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 討論. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 參考文獻. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 附圖. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 | |
dc.language.iso | zh-TW | |
dc.title | ?郭魚的兩型c-ski基因 | zh_TW |
dc.title | Two Distinct c-ski Gene of Fish, Tilapia (Oreochromis aurea) | en |
dc.date.schoolyear | 89-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 62 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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