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請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75218
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dc.contributor.authorJang-Yi Chenen
dc.contributor.author陳正繹zh_TW
dc.date.accessioned2021-07-01T08:12:15Z-
dc.date.available2021-07-01T08:12:15Z-
dc.date.issued1981
dc.identifier.citationBourgin, J. P. and J. D. Nitsch. 1967. Obtention de Nicotiana haploides a partir detamines cultivees in vitro. Ann. Physiol. Veg. 9: 377-382.
Chen, C. C. 1978. Effect of sucrose concentration on plant production in anther culture of rice. Crop. Sci. 18: 905-906.
Chen, C. M., C. C. Chen and M. H. Lin. 1981. Genetic analysis of anther drived plants of rice. in prepration.
Clapham, D. 1971. In vitro development of callus from the pollen of Lolium and Hordeum. Z. Pflanzenz?cht. 65: 285-292.
Clapham, D. 1973. Haploid Hordeum plants from anthers in vitro. Z. Pflanzenz?cht. 69: 142-155.
Devreux, M. and D. de Nettaucout. 1974. Screening mutation in haploid plants. In: Haploid in higher plants- advances and potential. (ed. K. J. Kasha) pp. 309-322. Guelph: The University of Guelph.
Dunwell, J. M. and N. Sunderland. 1973. Anther culture of Solanum tuberosum L. Euphytica 22: 317-323.
Dunwell, J. M. 1976. A comparative study of environmental and development factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environ. Exp. Bot. 16: 109-118.
En elman, J. and A. D. Hanson. 1971. Sucrose supression of chlorophyll synthesis in carrot callus culture. Planta 98: 150-156.
Gamborg, O. L. and D. E. Eveleigh. 1968. Culture methods and detection of glucanases in suspension cultures using dichromate oxidation. Can. J. Biochem. 46: 417-421.
Gresshoff, P. M. and C. H. Doy. 1972. Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 197: 161-170.
Guha, S. and S. C. Maheshwari. 1964. In vitro production of embryos from anthers of Datura. Nature 204: 497-499.
Guha, S. and S. C. Maheshwari. 1966. Cell division and differentiation of embryos in the pollen grain of Datura in vitro. Nature 212: 97-98.
Guha-Mukherjee, S. 1973. Genotypic differences in the in vitro formation of embryoids from rice pollen. J. Exp. Bot. 24: 139-144.
Heberle-Bors, E. and J. Reinert. 1979. Androgenesis in isolated pollen cultures of Nicotiana tabacum: dependence upon pollen development. Protoplasma 99: 237-245.
Horner, M. and H. E. Street. 1978. Pollen dimorphism-origin and significance in pollen plant formation by anther culture. Ann. Bot. 42: 763-771.
Kameya, T. and K. Hinata. 1970. Induction of haploid plants from pollen grains of Brassica. Jap. J. Breed. 20: 82-87.
Kasperbauer, M. J. and G. B. Collins. 1972. Reconstitution of diploids from leaf tissue of anther- drived haploids in tabacco. Crop. Sci. 12: 98-101.
Keller, W. A. and K. C. Armstrong. 1977. Embryogenesis and plant regeneration in Brassica napus anther cultures. Can. J. Bot. 55: 1383-1388.
Keller, W. A. and K. C. Armstrong. 1978. High frequency production of microspore-drived plants from Brassica napus anther cultures. Z. Pflanzenz?cht. 80: 100-108.
Keller, W. A. and K. C. Armstrong. 1979. Stimulation of embryogenesis and haploid production in Erassica campestris anther culture by elevated temperature treatments. Theor. Appl. Genet. 55: 65-67.
Keller, W. A., T. Rajhathy and J. Iacapra. 1975. In vitro production of plants from pollen In Brassica campestris. Can. J. Genet. Cytol. 17: 655-666.
Keller, W. A. and R. G. Stringam. 1978. Producing and utilization of microspore-derived haploid plants. In: Frontiers of plant tissue culture (ed. : Thorpe, T.A.), PP. 113-122. Calgary: IAPTCV.
Miao, S. H., C. S. Kuo, Y. L. Kwei, A. T. Sun, S. Y. Ku, W. L. Lu and Y. Y. Wang. 1978. Induction of pollen plants of maize and observations on their progeny. In: Proceedings of Symposium on Plant Tissue Culture. Peking, pp. 23-28. Peking: Science Press. Mitchell. A. Z., M. R. Hanson, R. C. Skvirsky and F. M. Ausubel. 1980. Anther culture of Petunia: genotypes with high frequency of callus, root, or plantlet formation. Z. Pflanzenphysiol. 100: 131-146.
Mondeil, F. 1974. Irradiation de microspores en culture d’anthers: essai d’une novelle technique d’obtention de mutations imm?diatement decelable et fixables (application a Nicotiana tabacum). Ann. Am?lior. Plantes 24: I-II.
Moore, P. and A. Maretzki. 1974. Anther culture. Annu Rep., Hawaii Sugar Planters’ Assoc. 1974: 31-32.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473-497.
Nakata, K. 1971. Competition among pollen grains for baploid tabacco plant formation by anther culture. I. Analysis with leaf color character. Jap. J. Breed. 21: 29-34
Nakata, K. and T. Kurinara. 1972. Competition among pollen grains for haploid tabacco plant formation by anther culture. II. Analysis with resistance to tacacco mosaic virus(TMV) and wildfire diseases, leaf color and leaf base shape characters. Jap. J. Breed. 22: 92-98.
Niizeki, H. and K. Oono. 1968. Induction of haploid rice plant from anther culture. Pro. Jap. Acad.
44: 554-557.
Nitsch, J. P. 1969. Experimental androgenesis in Nicotiana. Phytomorphology 19: 389-404.
Ono, H. and E. N. Larter. 1976. Anther culture of tritieale. Crop. Sci. 16: 120-122.
Onyang, T., H. Hu, C. Chang and C. Tsen. 1973. Induction of pollen plants from anthers of Triticum aestivum L. cultured in vitro. Sci. Sinica 16: 79-95.
Pamplin, E. J. and J. M. Chapman. 1975. Sucrose supression of chlorophyll synthesis in tissue culture: changes in the activity of the enzymes of chlorophyll biosynthetic pathway. J. Exp. Bot. 24: 212-220.
Reinert, J. and Y. P. S. Bajaj. 1977. Anther culture: haploid production and its significance. In: Applied fundamental aspects of plant cell, tissue ant organ. culture. (ed. J. Reinert and Y. P. S. Bajaj) pp. 251-267. Springer-Verlag, Berlin.
Sharp, W. R., D. K. Dougall and E. F. Paddock. 1971. Haploids plantlets and callus from immature pollen grains of Nicotiana and Lycopersicon. Bull. Torrey Bot. Club. 98: 219-222.
Sopory, S.K. 1979. Effect of sucrose, hormones and metabolic inhibitors on the development of pollen embryoids in anther culture of dihoploid Solanum tuberosum. Can J. Bot. 57:2691-2694.
Sopory, S. K., E. Jacobsen and G. Wenzel. 1978. Production of monohaploid embryoids and plantlets in cultured anthers of Solanum tuberosum. Plant Sci. Lett. 12: 47-54.
Sunderland, N. 1971. Anther culture: a progress report. Sci. Prog. Oxford 59: 527-549.
Sunderland, N. 1974. Anther culture as means of haploid induction. In: Haploid in higher plants- advances and potential. (ed. K. J. Kasha) pp. 91-122 Guelph: The University of Guelph.
Sunderland, N. and J. M. Dunwell. 1977. Anther and pollen culture. In: Plant tissue and cell culture. 2nd. □. (ed. H. E. Street) pp. 223-365. Oxford- London- □inburgh- Melbourne: Blackwell.
Sunderland, N. and F. M. Wicks. 1971. Embryoid formation in pollen grains of Nicotiana tabacum. J. Exp. Bot. 22: 213-226.
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75218-
dc.description.abstract以含3%、6%及9%蔗糖濃度的培養基培養菸草栽培 品種Wisconsin 38 (Nicotiana tabacum L. cv. Wisconsin 38 2n=48)的花藥,及含3%、6%、9%及12%蔗糖濃度培養基培養油菜栽培品種Tower (Brassica napus L. cv. Tower 2n=38)的花藥,以試驗培養基中蔗糖濃度對花粉植株形成的影響。在菸草中發現,當蔗糖濃度提高時:(1)各時期花粉發育成植株的速度普遍延遲。(2)較早期花藥(含早期單核至第一次有絲分裂的花粉)的反應百分率升高,但較晚期花藥(含雙核花粉)的反應百分率降低。(3)花藥內之植株數顯著減少。(4)癒合組織、畸形及白化苗的頻率增多。在油菜中發現,(l)當蔗糖濃度增高,由花絲產生癒合組織的頻率顯著下降,但培養基中蔗糖濃度高達12%時,仍有約20%的花藥產生花絲癒合組織。(2)有三個花藥在蔗糖濃度高於9%的培養基上產生了擬胚,其中兩個來自含晚期單核花粉的花藥,一個來自含第一次有絲分裂花粉的花藥;擬胚移至低蔗糖濃度的分化培養基,才能繼續發育。zh_TW
dc.description.abstractAnthers of Nicotiana tabacum L. cv. Wiscenuin 38 (2n=48) and Brassica napus L. cv. Tower (2n=38) were excised at Various developmental stages anc cultures on media containing various concentrations of sucrose. Within the range tested (3-9%), each increase in the level of sucrose in the tabacco anther culture medium caused: (1) an apparent delay in the time of plantlet emergence from the cultured anthers, (2) an increase in the frequency of responsive anthers when cultured at early uninucleate-first mitosis microspore stages and a decrease in the frequency of responsive anthers when cultured at the binucleate microspore stages,
(3) a reduction in the number cf plantlets per responsive anthers, and (4) the development of more callus, albino or abnormal plantlets from the microspores, Higher sucrose concentrations (9-12﹪) appeared to be essential for successful anther culture of Brassica napus, because they had promotive effects on embryoid formation from the microspores and inhibited callus formation from somatic tissues of the anther.
en
dc.description.provenanceMade available in DSpace on 2021-07-01T08:12:15Z (GMT). No. of bitstreams: 0
Previous issue date: 1981
en
dc.description.tableofcontentsⅠ 中文摘要………………………………………………………………………………1
Ⅱ 英文摘要………………………………………………………………………………2
Ⅲ 緒論………………………………………………………………………………3
Ⅳ 材料與方法………………………………………………………………………………7
Ⅴ 結果………………………………………………………………………………13
Ⅵ 討論………………………………………………………………………………26
Ⅶ 參考文獻………………………………………………………………………………33
dc.language.isozh-TW
dc.title培養基中蔗糖濃度對菸草及油菜花粉植株形成的影響zh_TW
dc.titleEffects of Sucrose Concentration on Plant Formation in Anther Cultures of Nicotiana tabacum and Brassica napusen
dc.date.schoolyear69-2
dc.description.degree碩士
dc.relation.page39
dc.rights.note未授權
dc.contributor.author-dept生命科學院zh_TW
dc.contributor.author-dept植物科學研究所zh_TW
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