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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Fu-Chen Hsu | en |
| dc.contributor.author | 許富誠 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:14Z | - |
| dc.date.available | 2021-07-01T08:12:14Z | - |
| dc.date.issued | 2001 | |
| dc.identifier.citation | Alfenito, M. R. and Birchler, J. A. (1993). Molecular characterization of a maize B chromosome centric sequence. Genetics 135, 589-597 Ananiev, E. V., Phillips, R. L. and Rines, H. W. (1998a). Complex structure of knob DNA on maize chromosome 9: Retrotransposon invasion into heterochromatin. Genetics 149, 2025-2037 Ananiev, E. V., Phillips, R. L. and Rines, H. W. (1998b). A knobassociated tandem repeat in maize capable of forming fold-back DNA segments: Are chromosome knobs megatransposons Proc. Natl. Acad. Sci. USA 95, 10785-10790 Ausubel, F. M., Brent, R., Moore, D. D., Sediman, J. G., Smith, J. A., and Struhl, K. (1987). Current Protocol in Molecular Biology (Greene Publishing associates and Wile-Interscience USA) Beadle, G. W. (1978). Teosinte and the origin of maize. In: Maize breeding and genetics. D. B. Walden, ed. (John Wiley & Sons, New York), pp. 113-128 Brown, W. L. (1949). Numbers and distribution of chromosome knobs in United States maize. Genetics 34, 524-536 Buckler IV, E. S., Phelps-Durr, T. L., Buckler, C. S. K., Dawe, R. K., Doebley, J. F., and Holtsford, T. P. (1999). Meiotic drive of chromosomal knobs reshaped the maize genome. Genetics 153, 415-426 Cai, M. and Davis, R. W. (1989). Purification of a yeast centromerebinding protein that is able to distinguish single base-pair mutations in its recognition site. Mol. Cell. Biol. 9, 2544-2550 Cai, M. and Davis, R. W. (1990). Yeast centromere binding protein CBF1, of the helix-loop-helix protein family, is required for chromosome stability and methionine prototrophy. Cell 61, 437-446 Chen, C. C., Chen, C. M., Hsu, F. C., Wang, C. J., Yang, J. T., and Kao, Y. Y. (2000). The pachytene chromosomes of maize as revealed by fluorescence in situ hybridization with repetitive DNA sequences. Theor. Appl. Genet. 101, 30-36 Chughtai, S. R. and Steffensen, D. M. (1987). Heterochromatic knob composition of commercial inbred lines of maize. Maydica 32, 171-187 Chughtai, S. R. and Steffensen, D. M. (1989). Knob constitution of inbred lines of maize (Zea mays L.) and its implications in maize breeding. Sabrao J. 21, 21-26 Dellaporta, S. (1994). Plant DNA miniprep and microprep: versions 2.1-2.3, In: The maize handbook, M. Freeling & V. Walbot, eds. (Springer-Verlag, New York), pp. 522-525 Dempsey, E. (1994). Traditional analysis of maize pachytene chromosomes. Fig. 66.1 In: The maize handbook, M. Freeling & V. Walbot, eds. (Springer-Verlag, New York), pp. 436 Dennis, E. S. and Peacock, W. J. (1984). Knob heterochromatin homology in maize and its relatives. J. Mol. Evol. 20, 341-350 de Wet, J. M. J. and Harlan, J. R. (1978). Tripsacum and the origin of maize. In: Maize breeding and genetics, D. B. Walden, ed. (John Wiley & Sons, New York), pp. 129-141 Fitzgerald, D. J., Dryden, G. L., Bronson, E. C., Williams, J. S., and Anderson, J. N. (1994). Conserved patterns of bending in satellite and nucleosome positioning DNA. J. Biol. Chem. 269, 21303-21314 Grellet, F., Delcasso, D., Panabieres, F., and Delseny, M. (1986) Organization and evolution of a higher plant alphoid-like satellite DNA sequence. J. Mol. Biol. 187, 495-507 Ibrahim, M. A. (1960). Asurrey of chromosome knobs in maize varieties. Genetics 45, 811-817 Ingham, L. D., Hanna, W. W., Baier, J. W., and Hannah, L. C. (1993). Origin of the main class of repetitive DNA within selected Pennisetum species. Mol. Gen. Genet. 238, 350-356 Kreike, C. M., de Koning, J. R. A., and Krens, F. A. (1990) Nonradioactive detection of single-copy DNA-DNA hybrids. Plant Mol. Biol. Rep. 8, 172-179 Longley, A. E. (1937). Morphological characters of teosinte chromosomes. J. Agric. Res. 54, 835-862 Longley, A. E. (1938). Chromosomes of maize from North American Indians. J. Agric. Res. 56, 177-195 Longley, A. E. (1939). Knob positions on corn chromosomes. J. Agric. Res. 59, 475-490 Longley, A. E. (1941). Chromosome morphology in maize and its relatives. Bot. Rev. 7, 263-289 Longley, A. E. (1945). Abnormal segregation during megasporogenesis in maize. Genetics 30, 100-113 Mangelsdorf, P. C. (1974). Corn, its origin, evolution and improvement. (Harvard University Press, Cambridge, Massachusetts) Martinez-Balbas, A., Rodriguez-Campos, A., Garcia-Ramirez, M., Sainz, J., Carrera, P., Aymami, J., and Azorin, F. (1990). Sattellite DNAs contain sequences that induce curvature. Biochemistry 29, 2342-2348 McClintock, B. (1929). Chromosome morphology in Zea mays. Science 69, 629 McClintock, B. (1941). The stability of broken ends of chromosome in Zea mays. Genetics 26, 234-282 Peacock, W. J., Dennis, E. S., Rhoades, M. M., and Pryor, A. J. (1981) Highly repeated DNA sequence limited to knob heterochromatin in maize. Proc. Natl. Acad. Sci. USA 78, 4490-4494 Reeves, R. G. (1944). Chromosome knobs in relation to the origin of maize. Genetics 29, 141-147 Riera-Lizarazu, O., Rines, H. W., and Phillips, R. L. (1996). Cytological and molecular characterization of oat ×maize partial hybrids. Theor. Appl. Genet. 93, 123-135 Rhoades, M. M. (1942). Preferential segregation in maize. Genetics 27, 395-407 Rhoades, M. M. and Dempsey, E. (1966). The effect of abnormal chromosome 10 on preferential segregation and crossing over in maize. Genetics 53, 989-1020 Rhoades, M. M. and Dempsey, E. (1973). Chromatin elimination induced by the B chromosome of maize. J. Hered. 64, 12-18 Rhoades, M. M. and Vilkomerson, H. (1942). On the anaphase movement of chromosomes. Genetics 28, 433-436 Rhoades, M. M., Dempsey, E., and Ghidoni, A. (1967). Chromosome elimination in maize induced by supernumerary B chromosomes. Genetics 57, 1626-1632 Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press). Solomon, M. J., Strauss, F., and Varshavsky, A. (1986) A mammalianhigh mobility group protein recognizes and stretch of six A-T base pairs in duplex DNA. Proc. Natl. Acad. Sci. USA 83, 1276-1280 Strauss, F. and Varshavsky, A. (1984). A protein binds to a satellite DNARepeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome. Cell 37, 889-901 Westneat, D. F., Noon, W. A., Reeve, H. K., and Aquadro, C. F. (1988). Improved hybridization conditions for DNA fingerprints probed with M13. Nucl. Acids Res. 16, 4161 Yu, H. G., Hiatt, E. N., Chan, A., Sweeney, M., and Dawe, R. K. (1997). Neocentromere-mediated chromosome movement in maize. J. Cell Biol. 139, 831-840 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75210 | - |
| dc.description.abstract | 異染色質結（heterochromatic knobs）是玉米細胞分裂前期（prophase）染色體上比較膨大、染色深的地方。其對於玉米遺傳上的影響主要是（1）具有新中節活性（neocentromere activity）以及（2）造成染色質丟失（chromatin elimination）。前人由玉米基因組中分離出兩種銜接排列的重複性序列（tandemly repeated sequences），180-bp序列及TR-1序列（350-bp），皆聚集在異染色質結。這兩種重複性序列中有兩段（分別為12 bp及31 bp）相似度在60％以上。而且發現180-bp與B染色體中節序列中的90 bp有72％的相似度，因此推測180-bp重複性序列可能與異染色質結具新中節活性有關。由於180-bp序列已經在玉米以及其近緣種中被詳細研究過，而有關TR-1的資料卻很少。因此本研究從不同的玉米品系及其近緣種分離出TR-1重複性序列，分析其分子結構。發現TR-1是由A、B/B'及C序列所組成的複合體，並且是以180 bp而不是350 bp為重複單位。B/B'與180-bp重複性序列有相似性的片段，推測B/B'的來源是180-bp序列。A、C序列由於：（1）兩者有高相似度的片段；（2）C序列的後面都接著A序列；（3）兩者A十T的含量都大約是70％；所以將C十A視為另一種180 bp的重複性序列。A、B/B'及C序列與B染色體中節序列也有許多相似性的片段，所以TR-1複合體在新中結活性上扮演重要的角色。另外，論文中也討論了TR-1複合體形成的可能機制。 | zh_TW |
| dc.description.abstract | Heterochromatic knobs are enlarged and deeply stained regions in prophase chromosomes of maize. The main genetic effects of knobs are (1) neocentromere activity and (2) chromatin elimination. Two classes of tandemly repeated sequences associated with knobs have been isolated from the maize genome, the 180-bp repeat and TR-1 element (350-bp). Two segments (12 bp and 31 bp) in these repeats show homology of more than 60%, and the 180-bp repeat has a 72% similarity to a 90-bp stretch of the maize B chromosome centromere sequence. It has been suggested that the 180-bp repeat may be responsible for neocentromere activity. The 180-bp repeat has been extensively studied in maize and its relatives, but the molecular structure of the TR-1 element is not clear. In this study, we isolated TR-1 elements from various lines of maize and its relatives and analysed their structures. Our results demonstrates that TR-1 element is composed of A, B/B', C repeats, and the unit length is 180 bp but not 350 bp as previously reported. There are some homology between segments of B/B' and the 180-bp repeat, so we presume that 180-bp repeat is the ancestor of B/B'. The following reasons suggest that C+A may be actually a class of repetitive sequence with a unit length of 180 bp: (1) A and C show high homology in nucleotide sequences; (2) C is always before A in the clones analysed; (3) A and C have the same percentage (approximately 70%) of A+T. The role of TR-1 in neocentromere activity and the mechanism of the formation of TR-1 are discussed. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2001 | en |
| dc.description.tableofcontents | 中文摘要…………………………………………………………………………………i 英文摘要…………………………………………………………………………………ii 壹、前言…………………………………………………………………………………1 貳、材料與方法…………………………………………………………………………5 一、植物材料……………………………………………………………………………5 二、植物總DNA的抽取……………………………………………………………………5 三、質體DNA的抽取………………………………………………………………………7 四、TR-1重複性序列的選殖與定序……………………………………………………8 五、南方氏雜交…………………………………………………………………………11 參、結果…………………………………………………………………………………13 一、TR-1是由A、B、C三種序列組成的複合體…………………………………………13 二、A序列的分析…………………………………………………………………………13 三、B序列的分析…………………………………………………………………………18 四、C序列的分析…………………………………………………………………………25 五、A、B、C三序列與其他序列的比對…………………………………………………25 六、基因組南方氏雜交…………………………………………………………………32 肆、討論…………………………………………………………………………………35 一、TR-1是由不同重複性序列組成的複合體…………………………………………35 二、TR-1在玉米演化過程中出現的時序………………………………………………35 三、TR-1是如何形成的…………………………………………………………………36 四、異染色質結中重複性序列與新中結活性的關係…………………………………39 五、異染色質結形成的原因……………………………………………………………41 伍、參考文獻……………………………………………………………………………42 附錄1、各選殖體A1序列之對齊排列……………………………………………………47 附錄2、各選殖體A2序列之對齊排列……………………………………………………48 附錄3、各選殖體B序列之對齊排列……………………………………………………49 附錄4、各選殖體2/3 B序列之對齊排列………………………………………………53 附錄5、各選殖體C序列之對齊排列……………………………………………………55 | |
| dc.language.iso | zh-TW | |
| dc.title | 玉米及其近緣種中TR-1重複性序列之分子結構 | zh_TW |
| dc.title | Molecular structures of TR-1 repetitive DNA sequences in maize and its relatives | en |
| dc.date.schoolyear | 89-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 55 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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