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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Nei-Yin Weng | en |
| dc.contributor.author | 翁乃茵 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:13Z | - |
| dc.date.available | 2021-07-01T08:12:13Z | - |
| dc.date.issued | 2001 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75207 | - |
| dc.description.abstract | CED-10屬於Rho family GTPases的成員。而線蟲的ced-2, ced-5, ced-10, 和ced-12基因調控了細胞屍體吞噬過程以及生殖器官頂端細胞（distal tip cells）的遷移過程。雖然活體外的實驗顯示，活化的Rac會引發Swiss 3T3纖維母細胞的細胞膜表面皺褶的形成，然而對於活體內Rac的功能尚有許多探討的空間。當異位且過量表達constitutively activated ced-10（ced-10V12）時，線蟲的六個感受機械性感覺細胞（mechanosensory touch receptor neurons）會不正常的生長；而當表達過量的ced-10基因時，則會出現不同於ced-10V12的突變表型，如在尾部出現多餘的感覺細胞。這可能是由於細胞譜系（cell lineage）遭到改變所致。 unc-73在C. elegans的細胞遷移過程（cell migration）以及指引神經走向的過程（axon guidance）扮演著重要的角色。藉由生化實驗，我們證明瞭UNC-73的第一個DH domain對CED-10/Rac1具有催化GDP置換成GTP的能力。除此之外，也證明瞭在活體外，UNC-73可以和CED-5結合，再加上我們的遺傳分析，我們推測，UNC-73可能連接上游的訊息，進一步去活化CED-10，促使C. elegans活體內細胞遷移的過程。 | zh_TW |
| dc.description.abstract | CED-10 belongs to Rho family GTPases. In C. elegans, ced-2, ced-5, ced-10 and ced-12 control phagocytosis of cell corpses and migration of gonadal distal tip cells. Although in vitro function of Rac has been shown to regulate a signal transduction pathway in Swiss 3T3 cells that links extracellular signals to the formation of lamellipodia, the in vivo function of Rac remains to be explored. I found that ectopic expression of constitutively activated ced-10 (designated ced-10V12) results in many defects in six touch cells in C. elegans. Interestingly, ced-10 overexpression causes different phenotype from that of ced-10V12, such as extra PLM-like cell body. This may due to the alteration of cell lineage. unc-73 is required for cell migrations and axon guidance in C. elegans. Biochemical experiment suggests that the first DH domain of UNC-73 activates CED-10. In addition, we showed that UNC-73 and CED-5 physically interact in vitro, together with our genetic data, raising the possibility that UNC-73 may link upstream signaling complexes and CED-10, mediates cell migration in C. elegans. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2001 | en |
| dc.description.tableofcontents | 致謝 中文摘要…………………………………………………1 英文摘要…………………………………………………2 Introduction……………………………………………3 Materials and Method…………………………………18 Results…………………………………………………23 Discussion………………………………………………31 References………………………………………………39 Figure……………………………………………………51 Table……………………………………………………56 | |
| dc.language.iso | zh-TW | |
| dc.title | UNC-73的in vitro功能及ced-10V12在線蟲touch cells所扮演的角色 | zh_TW |
| dc.title | In vitro function of UNC-73 and the role of ced-10V12 in C. elegans touch cells | en |
| dc.date.schoolyear | 89-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 58 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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