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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Yang Fure-Lin | en |
| dc.contributor.author | 楊福麟 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:03Z | - |
| dc.date.available | 2021-07-01T08:12:03Z | - |
| dc.date.issued | 1981 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75162 | - |
| dc.description.abstract | Based upon its ability to inhibit the contractions of electrically stimulated ileum, opioid peptide and its precursor have been isolated from porcine pituitary glands by extraction into acetone, partial fractionation by NaCl, ion-exchange and high performance liquid chromatography. When the component V-2 (with a molecular weight around 14,000) is subjected to trypsinization, its tryptic products cause a profound, shortlasting inhibitory effect on guinea pig ileum. The inhibitory effect can be completely antagonized by naloxone, an antagonist to narcotics. Moreover, this component possesed oxytocic activity on the rat uterus, a target organ of oxytocin. The active fragments for opioid activity are isolated and the amino acid composition is presented. Although additional work is required to confirm their biological activity, components XIII-d and XIII-c have amino acid compositions close to that of ACTH. In this study high performance liquid chromatography has been used for the isolation and purification of proteins and peptides with a wide range of molecular weights. The results are very satisfactory and demonstrate the great potential of this technique in protein chemistry. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:03Z (GMT). No. of bitstreams: 0 Previous issue date: 1981 | en |
| dc.description.tableofcontents | List of Abbreviations……(iii) List of Tables……(iv) List of Figures……(v) Summery……(vii) 1 Introduction I Enkephalin……１ II Endorphin……6 III β-lipotnopin……9 IV Commor, Preoursors for ACTH, β-LPH, β-MSH, CLIF, ＆-MSH, r-LPH, β-Endorphin, β-MSH and r-MSH……10 V The Precunsons for Methionine-enkephelin and Leucine-enkephalir……12 VI Methods of Assaying Opioid Activity……16 2 Materials and Methods I Meterials and equipments……17 II Prereration and Fartiel Fractionation of Acidacetone Fowder……18 III Chromatography……21 IV Electrophoresis (1) Disoontinuous Polyacrylamide Gel Electophoresis……22 (2) SDS-Polyacrylamide Gel Electrophoresis For Detenmination of Molecular Weight……23 V High Perfonmance Liquid Chromatography……26 VI Trypsinization……28 VII Assay System for Opioid Activity……29 VIII Analysis of Amino Acid Composition……30 3 Results I Preparation of Acid-acetone Powder (AAF) and Partial Fractiontion of AAF……32 II Fractionation of Fraction C-D by Ion-exchange Chromatography on Carboxymethyl Cellulose Column ……32 III Purification of fraction V from CMC Chromatography by High Performance Liquid Chromatography……36 IV Purification of Opicid Peptides (active fragments) after Trypsinization of Component V-2……51 V Purifioation of Fraction XIII from CMC Chrometogranphy by High Performance Liquid Chromatography……59 4 Disoussion……63 5 Summary in Chinese……69 6 Acknonledgments……70 7 References……71 | |
| dc.language.iso | zh-TW | |
| dc.title | 從豬腦下腺分離腎上腺皮質劑刺激素和類嗎啡作用勝?之研究 | zh_TW |
| dc.title | STUDIIES ON THE ISOLATION OF ACTH AND OPIOID PEPTIDES FROM PORCINE PITUITARY GLANDS | en |
| dc.date.schoolyear | 69-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 76 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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