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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75136完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Ting-Hsuan Chiang | en |
| dc.contributor.author | 江亭萱 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:57Z | - |
| dc.date.available | 2021-07-01T08:11:57Z | - |
| dc.date.issued | 2000 | |
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Ultrastructure of the testis and spermatogenesis in Brachymystax lenok (pallas). Acta Zoologica Sinica., 38(4), 355-358 72. Zirkin, B.R. (1975). The ultrastructure of nuclear differentiation during spermiogenesis in the Salmon. J.Ultrastructure Research., 50, 174-184 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75136 | - |
| dc.description.abstract | 本研究利用組織化學染色、超薄切片，以及透過Tanaka所開發出來的Osmium-DMSO-Osmium（O-D-O）冷凍斷裂技術等方法處理樣品，並在光學顯微鏡及電子顯微鏡下，觀察描述蓋斑門魚成熟精巢之形態結構。根據實驗觀察結果，蓋斑捫魚雄魚精巢為細長條狀，呈略帶透明的乳白色。內部由許多管狀構造（細精管）所組成，成樹枝狀排列。由於細精管中無次序散佈許多不同發育階段生殖細胞的包囊，因此判定蓋斑門魚精巢為小葉型（lobule type）。精子成熟的過程（spermatogenesis）可粗分為兩個階段：一為精細胞的發育（spermatocytogenesis），二為精子的變態（spermiogenesis）。蓋斑門魚精細胞的發育是由初、次級精原細胞（spermatogonia）經有絲分裂形成精母細胞（spermatocyte），而後再經減數分裂形成精細胞（spermatid），過程中細胞型態的變化包含細胞逐漸縮小，以及細胞質內胞器組成及結構上的改變。分裂完成後形成的精細胞再經過精子變態的過程，最後生成具有活力的成熟精子。關於精子變態的過程，依據精細胞中心粒所在的區域、鞭毛的生成、中心粒與細胞核間的相關位置及細胞質的脫落可區分成五個時期；第一期：早期精細胞。中心粒遠離細胞膜而與其他胞器共同存在於細胞質中。第二期：鞭毛形成期。中心粒向細胞膜移動並接觸，而後進行鞭毛生長。第三期：中心粒－細胞核複合體形成期。中心粒與細胞核相接觸乃至於陷入細胞核底部凹陷。第四期：細胞質的丟棄。精細胞細胞質中出現大量空泡，且Sertoli cell細胞質突起包圍精細胞，用以輔助其細胞質丟棄的進行。第五期：精子期。精子頭部為卵圓形，無頂體（acrosome），細胞核內染色質相當緻密聚集，尾部鞭毛為典型9＋2微管構造。另外，精巢間質組織（interstitial tissue）當中也存在著許多不同的細胞，其細胞類型包括有myoid boundary cells、萊氏細胞（Leydig cells）等，各細胞擁有不同的形態結構，則分別扮演著不同的生理機能。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
| dc.description.tableofcontents | 摘要………………………………………………………………i 目次……………………………………………………………iii 前言……………………………………………………………1 1．緒論…………………………………………………………1 2．精巢之外觀…………………………………………………2 3．精巢之組織構造……………………………………………3 4．精子生成……………………………………………………4 5．Sertoli cell………………………………………………9 6．間質組織區細胞……………………………………………10 7．蓋斑門魚簡介………………………………………………11 材料與方法……………………………………………………12 1．光學顯微鏡樣品製備及組織化學染色……………………12 2．穿透式電子顯微鏡之樣品製備……………………………13 3．掃描式電子顯微鏡之樣品製備……………………………13 4．生殖細胞之大小測量………………………………………14 結果……………………………………………………………15 1．蓋斑門魚雄性生殖腺之外觀………………………………15 2．精巢之組織結構……………………………………………15 3．精子生成……………………………………………………17 4．間質組織區細胞結構………………………………………25 討論……………………………………………………………27 1．精巢的構造…………………………………………………27 2．精細胞的發育………………………………………………27 3．精子變態……………………………………………………32 參考文獻………………………………………………………40 圖表及說明……………………………………………………49 | |
| dc.language.iso | zh-TW | |
| dc.title | 蓋斑門魚精巢結構：光學及電子顯微鏡之研究 | zh_TW |
| dc.title | Light and Electron Microscopic Study on the Testis of Paradise Fish，Macropodus opercularis | en |
| dc.date.schoolyear | 88-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 93 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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