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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Yung-Ting Lin | en |
| dc.contributor.author | 林詠婷 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:52Z | - |
| dc.date.available | 2021-07-01T08:11:52Z | - |
| dc.date.issued | 2000 | |
| dc.identifier.citation | 王敏鈺1995一株綠島海濱熱泉好熱性脫氮性細菌之研究。台灣大學海洋所碩士論文。 莊榮輝、蘇仲卿1986蛋白質膠體電泳檢定法。電泳分離技術研討會論文pp69-85。 陳家全、李家維、楊瑞森1991生物電子顯微鏡學。行政院國科會精密儀器中心編印。臺北。pp63-69。 張寶堂1995台灣溫泉與地熱探勘。能源、資源與環境8(3):35-42。 陳端青1992a台灣菌類資源調查之歷史與現況。「台灣生物資源調查及資訊管理研習會」論文集（彭鏡毅編），中央研究院植物研究所專刊十一號，pp119-130。 陳桂玉1992b台灣產嗜熱及耐熱性真菌分類學之研究台灣大學植物所博士論文。 楊麗芳、林俊宏2000台灣溫泉之旅。生活情報文化事業股份有限公司。臺北。pp5-10。 楊美桂、陳淵銓1995普通微生物學實驗。藝軒圖書出版社。臺北。pp63-69。 簡文達1993綠島沿岸熱泉中好氧性脫氮細菌之研究。台灣大學海洋所碩士論文。 簡文達1998綠島沿岸熱泉中度好熱性異營細菌之研究。台灣大學海洋所博士論文。 譚建安、蔡珊珊1985 Bacillus licheniformisB7的細胞外耐熱性澱粉液化?。中華微免雜誌。18(1):44-53。 Aguilar, A., T. lngemansson, and E. Magnien. 1998. Extremophile microorganisms as cell factories: support from the European Union. Extremophiles 2: 367-373. Barelli, H., J. P. Vincent, and F. Checler. 1988. Peripheral inactivation of neurotensin. Isolation and characterization of a metallopeptidase from rat ileum. Eur. J. Biochem. 175(3): 481-489. Barker, D., M. Hoff, A. Oliphant, and R. White. 1984. A second type II restriction endonuclease from Thermus aquaticus with an unusual specificity. Nucleic Acids Res. 12: 5567-5581. Beveridge, T. J. 1990. Mechanism of gram variability in select bacteria. J. Bacteriol. 172(3): 1609-1620. Bl?chl, E., R. Rachel, S. Burggraf, D. Hafenbradl, H. W. Jannasch and K. O. Stetter. 1997. Pyrolobus fumarii, gen. and sp. nov., represents a novel group of archaea, extending the upper temperature limit for life to 113℃. Extremophiles 1: 14-21. Bouthier de la Tour C., C. Portemer, M. Nadal, K. O. Stetter, P. Forterre, and M. Duguet. 1990. Reverse gyrase, a hallmark of the hyperthermophilic archaebacteria. J. Bacterial. 172: 6803-6808. Bradford, M. M. 1976. A rapid and sensitive method of the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248-254. Breccia, J. D., F. Sineriz, M. D. Baigori, G. R. Castro, and R.H. Kaul. 1998. Purification and characterization of a thermostable xylanase from Bacillus amyloliquefaciens. Enzyme Microb. Technol. 22: 42-49. Brock, T. D. and H. Freeze. 1969. Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J. Bacteriol. 98: 289-297. Brock, T. D. and K. L. Boylen. 1973. Presence of thermophilic bacteria in laundry and domestic hot water heaters. Appl. Microbiol. 25: 72-76. Cao, W., J. Lu, S. G. Welch, R. A. D. Williams, and F. Barany. 1998. Cloning and thermostability of Taql endonuclease isoschizomers from Thermus species SM32 and Thermus filiformis Tok6Al. Biochem. J. 333: 425-431. Cappuccino J. G. and N. Sherman. 1992. Microbiology : a laboratory manual. Benjamin/Cummings Publishing Company, Inc. Redwood city, California. Chien, A., D. B. Edgar, and J. M. Trela. 1976. Deoxyribonucleic acid polymerase from extreme thermophile Thermus aquaticus. J. Bacteriol. 127:1550-1555. Colman, P. M., J. N. Jansonius, and B. W. Matthews. 1972. The structure of thermolysin and electron density map at 2.3? resolution. J. Mol. Biol. 70: 701-724. Cowan, D. A. and R. M. Daniel. 1996. Rapid purification of two thermophilic proteinases using dye-ligand chromatography. J. Biochem. Biophys. Methods. 32: 1-6. DeRose M., A. Gambacorta, R. Huber, V. Lanzotti, B. Nicolaus, K. O. Stetter, and A. Trincone. 1989. Lipid structures in Thermotoga maritima. In Da Costa M. S., J. C. Duarte, and R. A. D. Williams (eds.). Microbiology of Extreme Environments and Its Potential for Biotenchnology. London and New York: Elsevier Applied Science, pp. 167-173. Edmonds C. G., P. F. Cram, R. Gupta, T. Hashizume, C. H. Hocart, S. C. Kowalak, S. C. Pomerantz, K. O. Stetter, and J. A. McCloskey. 1991. Posttranscriptional modification of tRNA in the thermophilic archaea (archaebacteria). J. Bacteriol. 173: 3138-3148. Grayling R. A., K. Sandman, and J. N. Reeve. 1994. Archaeal DNA binding proteins and chromosome structure. In Pfeifer F., P. Plam, and K. H. Schleifer (eds): Molecular Biology of Archaea. Stuttgar-Jena-New York: Gustav Fischer Verlag, pp. 82-90. Hase, C. C. and R. A. Finkelstein. 1993. Bacterial extracellular zinc-containing metalloprotease. Microbiol. Rev. 57(4): 823-837. Herbold, D. R. and L. Glaser. 1975a. Bacillus subtilis N-acetylmuramic acid L-alanine amidase. J. Bid. Chem. 250: 1676-1680. Herbold, D. R. and L. Glaser. 1975b. Interaction of N-acetylmuramic acid L-alanine amidase with cell wall polymers. J. Biol. Chem. 250: 7231-7238. Heussen, C. and E. B. Dowdle. 1980. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal. Biochem. 102: 196-302. Holmes, M. A. and B. W. Matthews. 1982. Structure of thermolysin refined at 1.6? resolution. J. Mol. Bid. 160: 623-639. Holt, J. G., editor. 1984. Bergey's manual of systematic bacteriology. 1 vol. Baltimore, Md.: Wialliams & Wilkins. pp. 333-337. Holt, J. G., editor. 1986. Bergey's manual of systematic bacteriology. 2 vol. Baltimore, Md.: Wialliams & Wilkins. pp. 1138. Horikoshi, K. and W. D. Grant. 1998. Extremophiles: Microbial life in extreme environments. Wiley-Liss, Inc. Canada. Jongeneel, C. V., J. Bouvier, and A. Bairoch. 1989. A unique signature identifies a family of zinc-dependent metallopeptidases. FEBS Lett. 242: 211-214. Kates, M. 1992. Archaebacterial lipids: structure, biosynthesis and function. In Danson M. J., D.W. Hough, and G. G. Lunt (eds.). The Archaebacteria: Biochemistry and Biotechnology. London and Chapel Hill: Portland Press, pp. 51-72. Kawai, G., T. Hashizume, M. Yasuda, T. Miyazawa, J. A. McCloskey, and S. Yokoyama. 1992. Conformational rigidity of N4-acetyl-2'-O-methylcytidine found in tRNA of extremely thermophilic archaebacteria (Archaea). Nucleosides Nucleotides 11(1-4): 759-771. Kikuchi, A. and K. Asai. 1984. Reverse gyrase-a topoisomerase which introduces positive superhelical turns into DNA. Nature 309: 677-681. Lin, L. L., M. R. Tsau, and W. S. Chu. 1994. General characteristics of thermostable amylopullulanase and amylases from the alkalophilic Bacillus sp. TS-23. Appl. Microbiol. Biotechnol. 42: 51-56. Lin, L. L., M. R. Tsau, and W. S. Chu. 1996. Purification and properties of a 140 kDa amylopullulanase from thermophilic and alkaliphilic Bacillus sp. strain TS-23. Biotechnol. Appl. Biochem. 24: 101-107. Lin, L. L., C. C. Chyau, and W. H. Hsu. 1998. Production and properties of a raw-starch-degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS-23. Biotechnol. Appl. Biochem. 28: 61-68. Lin, X., C. G. Lee, E. S. Casale, and J. C. H. Shih. 1992. Purification and characterization of a keratinase from a feather-degrading Bacillus lichen/form/s strain. Appl. Environ. Microbiol. 58(10): 3271-3275. Loginova, L. A. and L. A. Erorgova. 1975. An obligately thermophilic bacterium Thermus ruber from hot springs in Kamchatka. Mikrobiologia 44: 661-665. Lu, Y. M., J. J. Tsai and S. S. Tsay. 1991. Enzymatic saccharification alkaline-treated rice straw. J. Chinese Agric. Chem. Soc. 29(1): 33-41. Lu, Y. M. and S. S. Tsay. 1991. Hydrolysis of alkaline-treated rice straw by the adsorbed cellulases. J. Chinese Agric. Chem. Soc. 29(1): 74-86. Miyakawa, H., S. Kobayashi, S. Shimamura, and M. Tomita. 1991. Purification and characterization of an X-prolyl dipeptidyl aminopeptidase from Lactobacillus delbrueckliisp. bulgaricus LBU-147. J. Dariy. Sci. 74(8): 2375-2381. Neurath, H. 1984. Evolution of proteolytic enzymes. Science. 224: 350-357. Nongporn, H. T., P. Anongnat, and S. Prasert. 1999. Purification and characterization of an extracellular protease from alkaliphilic and thermophilic Bacillus sp. PS719. J. Biosci. Bioengin. 87(5): 581-587. Oshima, T. and K. Imahori. 1971. Isolation of an extreme thermophile and thermostability of its transfer ribonucleic acid and ribosomes. J. Gen. Appl. Microbiol. 17: 513-517. Oshima, T. and K. Imahori. 1974. Description of Thermus the thermophilic (Yoshida and Oshima) camb. nov. a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacterial. 24: 102-112. Pask-Hughes, R. A. and R. A. D. Williams. 1975. Extremely thermophilic Gram-negative bacteria from hot tap water.J. Microbiol. 88: 321-328. Pask-Hughes, R. A. and R. A. D. Williams. 1977. Yellow-pigmented strains of Thermus spp. from Icelanic hot springs. J. Gen. Microbiol. 102: 375-383. Pask-Hughes, R. A. and R. A. D. Williams. 1978. Cell envelope components of strains belong to the Genus Thermus. J. Gen. Microbiol. 107: 65-72. Ramaley, R. A. and J. Hixson. 1970. Isolation of a non-pigmented, thermophilic bacterium similar to Thermus aquaticus. J. Gen. Microbiol. 103: 527-528. Reddy T. and T. Suryanarayana. 1988. Novel histone-like DNA-binding proteins in the nucleoic from the acidothermophilic archaebacterium Sulfolobus acidocaldarius that protect DNA against thermal denaturation. Biochim. Biophys. Acta. 949: 87-96. Ricardo Chavira, J. T. J. Burnett, and J. H. Hageman. 1984. Assaying proteinase with azocoll. Anal. Biochem. 136: 446-450. Saiki, T., R. Kimura, and K. Arima. 1972. Isolation and characterization of extreme thermophilic bacteria from hot springs. Agric. Biol.Chem. 36: 2357-2366. Salvesen, G. and H. Nagase. 1989. In Beynon, R. J. and J. S. Bond, (ed.) Proteolytic enzymes: a practical approach. IRL Press, Oxford. Sandman K., J. A. Krzycki, B. Diorinski, R. Lurz, and J. N. Reeve. 1990. DNA binding protein HMf, isolated from the hyperthermophilic archaeon Methanothermus fervidus, is most closely related to histones. Proc. Natl. Acad. Sci. USA 87: 5788-5791. Sato. S., C. A. Hutchison and J. I. Harris. 1977. A themostable sequence specific endonuclease from Thermus aquaticus. Proc. Natl. Acad. USA 74: 542-546. Shinomiya. T. and S. Sato. 1980. A site specific endonuclease from Thermus thermophilus 111, Tth 111I. Nucleic Acids Res. 8: 43-56. Shinomiya. T., M. Kobayashi, and S. Sato. 1980. A second site specific endonuclease from Thermus thermophilus 111, Tth 111 II. Nucleic Acids. Res 8. 3275-3285. Stainthorpe, A. C. and R. A. D. Williams. 1998. Isolation and properties of Clostridium thermocellum from Icelandic hot springs. J. Syst. Bacteriol. 38: 119-121. Stark, W., R. A. Pauptit, K. S. Wilson, and J. N. Jansonius. 1992. The structure of neutral protease from Bacillus cereus at 0.2 nm resolution. Eur. J. Biochem. 207: 781-791. Stetter, K. O. and W. Zillig. 1985. Thermoplasma and the thermophilic sulfurdependent archaebacteria. In Wolfe R. S. and C. R. Woese (eds.). The Bacteria, Volume VIII. New York: Academic Press, pp. 85-170. Stetter K. O., G. Fiala, G. Huber, R. Huber, and A. Segerer. 1990. Hyperthermophilic microorganisms. FEMS Microbial. Rev. 75: 117-124. Stetter, K. O. 1998. Hyperthermophiles: isolation, classification and properties. Extremophiles: Microbiol Life in Extreme Environments, Wiley-Liss, Inc. pp. 1-24. Stetter, K. O. 1999. Extremophiles and their adaptation to hot environments. FEBS. Lett. 452: 22-25. Sunna, A., S. G. Prowe, T. Stoffregen, and G. Antranikian. 1997. Characterization of the xylanase from the new isolated thermophilic xylan-degrading Bacillus thermoleovorans strain K-3d and Bacillus flavothermus strain LB3A. FEMS Microbiol. Lett. 148: 209-216. Sunna, A., S. Tokajian, J. Burghard, F. Rainey, G. Antranikian, and F. Hashwa. 1997. Identification of Bacillus kaustophilus, Bacillus thermocatenulatus and Bacillus strain HSR as members of Bacillus thermoleovorans. System Appl. Microbial. 20: 232-237. Tachibana, Y., A. Kuramura, N. Shirasaka, Y. Suzuki, T. Yamamoto, S. Fujiwara, M. Takagi, and T. Imanaka. 1999. Purification and characterization of an extremely thermostable cyclomaltodextrin glucanotransferase from a newly isolated hyperthermophilic archaeon, a Thermococcus sp. Appl. Environ. Microbiol. 65: 1991-1 997. Thayer, M. M., K. M. Flaherty, and D. B. Mckay. 1991. Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5A resolution. J. Biol. Chem. 266: 2864-2871. Valle, B. L. and D. S. Auld. 1990a. Active-site zinc ligands and activated H2O of zinc enzyme. Proc. Natl. Acad. Sci. USA 87: 220-224. Valle, B. L. and D. S. Auld. 1990b. Zinc coordination, function, and structure of enzymes and other proteins. Biochemistry. 29: 5647-5659. Viikari, L., A. Kantelinen, J. Sundquist, and M. Linko. 1994. Xylanase in bleaching: from an idea to the industry. FEMS Microbiol. Rev. 13: 335-350. Wandersman, C. 1989. Secretion, processing and activation of bacterial extracellular protease. Mol. Microbiol. 3: 1825-1 831. Wang, J. J. and J. C. H. Shih. 1999. Fermentation production of keratinase from Bacillus licheniformis PWD-1 and a recombinant B. subtilis FDB-29. J. Indust. Microbiol. & Biotechnol. 22: 608-616. Williams, C. M., C. S. Richter, J. M. Mackenzie, J. R. and J.C. H. Shih. 1990. Isolation, identification, and characterization of a feather-degrading bacterium. Appl. Environ. Microbiol. 56: 1509-1515. Williams, R. A. D., K. E. Smith, S. G. Welch, and J. Micallef. 1996. Thermus oshimai sp. nov., isolation from hot springs in Portugal Iceland, and the Azores, and comment on the concept of a limited geographical distribution on Thermus species. Int. J. Syst. Bacteriol. 46(2): 403-408. Wolz, R. L. and R. Zwilling. 1989. Kinetic evidence for copperative binding of two orthophenanthroline molecules to astacus protease during metal removal. J. Inorg. Biochem. 35(3): 157-167. Woese C. R., L. Achenbach, P. Roviere, and L. Mandelco. 1991. Archaeal phylogeny: reexamination of the phylogenetic position in light of certain composition-induced artifacts. System. Appl. Microbiol. 14: 364-371. Zarilla, K. A. and J. J. Perry. 1987. Bacillus thermoleovorans, sp. nov., a species of obligately thermophilic hydrocarbon utilizing endospore-forming bacteria. System. Appl. Microbiol. 9: 258-264. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75113 | - |
| dc.description.abstract | 本實驗由台灣北部溫泉和其他地熱環境分離得到80株嗜熱細菌。將這些菌株純化後，先做初步外泌酵素（蛋白?、澱粉水解?、脂質水解?）測試，選取其中9株做形態及生理特性之分析，並依據165 rDNA基因序列加以鑑定。其中7株會產生內抱子的被鑑定為 Bacillus，另有兩株不產生內孢子的則是Thermus。一株自烏來溫泉中篩選出的好氧嗜熱菌，Bacillus thermocatenulatu, WL65，被桃選來進行本實驗。將此菌株在添加typtone和yeast extract於Thermus基本培養基，於55℃下培養，會產生外泌性蛋白?。此株嗜熱菌具有周圍鞭毛和革蘭氏染色呈現Gram variable的現象。將此菌大量培養去除菌體後，將粗酵素液以硫酸銨沉澱、疏水性管柱層析、陰離子交換樹脂管柱層析進行純化。得到此蛋白?的比活性為25.83U/mg蛋白質，其分子量約65kDa；酵素最適當作用的酸鹼值在pH9,最適作用溫度在37℃；即使將溫度提高到50℃時，酵素仍保有95%以上的活性。此蛋白?活性會被EDTA所抑制，添加1 mM Cu(上標 2+)會使酵素活性增加。由這些實驗結果得知，此酵素為熱穩定型之鹼性蛋白?，銅的添加可以促進酵素活性。 | zh_TW |
| dc.description.abstract | Eighty strains of thermophilic bacteria were isolated from hot springs and geothermal environment of Northern Taiwan. Nine strains of these isolates were selected by preliminary analysis of extracellular enzymes (protease, lipase, and amylase) productions. Conventional morphological and physiological characteristics as well as analysis based on 16S rDNA gene sequences were examined for the identification of these microorganisms. Seven endospore producing strains were identified as Bacillus. The other two non-spore forming isolates were identified as Thermus. Bacillus thermocatenulatus WL 65, isolated from a hot spring in Wu-lai, was selected for this study. This strain produced protease into extracellular medium when grown on Thermus medium containing tryptone and yeast extract at 55℃. This aerobic thermophile had peritrichous flagella and stained Gram-variable. Protease was purified from the culture supernatant by using ammonium sulfate precipitation, hydrophobic interaction chromatography, and anion exchange adsorption. The specific activity of the purified protease was 25.83 U/mg protein. The molecular weight determinted by 10% SDS-PAGE was about 65 kDa. The enzyme had maximum activity at pH 9.0 and 37℃, and more than 95% enzymatic activity was retained when temperature was raised to 50℃. The proteolytic activity was inhibited by EDTA. The activity was stimulated in the presence of 1 mM Cu(superscript 2+). These experimental results indicated that the enzyme was a thermostable alkaline protease, the addition of copper will stimulate enzyme activity. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………Ⅰ 英文摘要………………………………………………………………Ⅱ 前言……………………………………………………………………1 材料與方法…………………………………………………………8 結果……………………………………………………………………29 討論……………………………………………………………………37 結論……………………………………………………………………44 參考文獻………………………………………………………………45 圖表……………………………………………………………………54 附錄一…………………………………………………………………79 附錄二…………………………………………………………………82 | |
| dc.language.iso | zh-TW | |
| dc.title | 台灣鳥來溫泉嗜熱細菌Bacillus thermocatenulatus WL 65之篩選 | zh_TW |
| dc.title | Isolation and Characterization of a thermophile Bacillus thermocatenulatus WL 65 form a hot spring in Wu-lai, Taiwan | en |
| dc.date.schoolyear | 88-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 85 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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