請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75091
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 王子銘 | zh_TW |
dc.date.accessioned | 2021-07-01T08:11:46Z | - |
dc.date.available | 2021-07-01T08:11:46Z | - |
dc.date.issued | 2000 | |
dc.identifier.citation | Ahmad, I. (1995). Mash-l is expressed during ROD photoreceptor differentiation and binds an E-box, Eopsin-1 in the rat opsin gene. Developmental Brain Research 90, 184-189.
Archer, S. N. and Hirano, J. (1998). Rod opsin sequence in John Dory: further evidence for the spectral tuning of rhodopsin. Journal of Fish Biology 52, 209-212. Baehr, W., Falk, J. D., Bugra, K., Triantaafyllos, J. T. and McGinnis, J. F. (1988). Isolation and analysis of the mouse opsin gene. FEBS Letters 238, 253-256. Batni, S., Scalzetti, L., Moody, S. A. and Knox, B. E. (1996). Characterization of the Xenopus rhodopsin gene, The Journal of Biological Chemistry 271, 3179-3186. Berne, R. M. and Levy, M. N. (1996). In: Principles of Physiology, 2nd ed, Mosby-Year Book, Missouri, USA. pp. 113-115. Bowmaker, J. K., Dartnall, H. J. A. and Mollon, J. D. (1980). Micro-spectrophotometric demonstration of four classes of photoreceptors in an old world primate Macaca fascicularis. Journal of Physiology (London) 298, 131-143. Chen, N., Ma, J. X., Corson, D. W., Hazard, E. S. and Grouch, R. K. (1996). Molecular cloning of a rhodopsin gene from salamander rods. Investigative Ophthalmology & Visual Science 37, 1907-1913. Chen, S., Wang, Q. L., Nie, Z., Sun, H., Lennon, G., Copeland, N. G., Gilbert, D. J., Jenkins, N. A. and Zack, D. J. (1997). Crx, a novel Otx-like paired-homeodomain protein, binds to and transactivates photoreceptor cell-specific genes. Neuron 19, 1017-1030. Chen, S. and Zack, D. J. (1996) Ret 4, a positive acting rhodopsin regulatory element identified using a bovine retina in vitro transcription system. The Journal of Biological Chemistry 271, 28549-28557. DesJardin, L. E. and Hauswirth, W. W. (1996). Developmentally important DNA elements within the bovine opsin upstream region. Invesigative Ophthalmology & Visual Science. 37, 154-165. DesJardin, L. E., Timmers, A. M. and Hauswirth, W. W. (1993). Transcription of photoreceptor genes during fetal retinal development. Evidence for positive and negative regulation. The Journal of Biological Chemistry 268, 6953-6960. Dryja, T. P., McGee, T. L., Reichel, E., Hahn, L. B., Cowley, G. S., Yandell, D. W., Sandberg, M. A. and Berson, E. L. (1990). A point mutation of the rhodopsin gene in one form of retinitis pigmentosa. Nature 343, 364-366. Fei, Y., Matragoon, S., Smith, S. B., Overbeek, P. A., Chen, S., Zack, D. J. and Liou, G. I. (1999). Functional dissection of the promoter of the interphotoreceptor retinoid-binding protein gene: the cone-rod-homeobox element is essential for photoreceptor-specific expression in vivo. The Japanese Biochemical Society 125, 1189-1199. Hall, M. D., Hoon, M. A., Ryba, N. J. P., Pottinger, J. D. D., Keen, J. N., Saibil, H. R. and Findlay, J. B. C. (1991). Molecular cloning and primary structure of squid (Loligo forbesi) rhodopsin, a phospholipase C-directed G-protein-linked receptor. Biochemistry Journal 274, 35-40. Hisatomi, O., Iwasa, T., Tokunaga, F. and Yasui, A. (1991). Isolation and characterization of lamprey rhodopsin cDNA. Biochemical and Biophysical Research Communications 174, 1125-1132. Hsiung, H. C. (2000). Molecular cloning and structure analysis of the rhodopsin genes of tuna (Tunnus tonggol) and deep-sea black dragonfish (Idiacanthus antrostmus). Master thesis, National Taiwan University, Taipei, Taiwan. Inoue, H., Nojima, H. and Okayama, H. (1990). High efficiency transformation of Escherichia coli with plasmids. Gene 96, 23-28. Iwamatsu, T. (1994). Stages of normal development in the medaka Oryzias latipes. Zoological Society of Japan 11, 825-839. Johnson, R. L., Grant, K. B., Zankel, T. C., Boehm, M. F., Merbs, S. L., Nathans, J. and Nakanishi, K. (1992). Cloning and expression of goldfish opsin sequences. Biochemistry 32, 208-214. Kawamura, S. and Yokayama, S. (1994). Cloning of the rhodopsin-encoding gene from the rod-less lizard Anolis carolinensis. Gene 149, 267-270. Khorana, H. G. (1992). Rhodopsin, photoreceptor of the rod cell. The Journal of Biological Chemistry 267, 1-4. Kinoshita, M. and Ozato, K. (1995). Cytoplasmic microinjection of DNA into fertilized medaka (Oryzias latipes) eggs. The Fish Biology Journal MEDAKA 7, 59-64. Knox, B. E., Schlueter, C., Sanger, B. M., Green, C. B. and Besharse, J. C. (1998). Transgene expression in Xenopzis rods. FEBS Letters 423, 117-121. Kuhn, H. and Wilden, U. (1987). Deactivation of photoactivated rhodopsin by rhodopsin-kinase and arrestin. Journal of Receptor Research 7, 283-298. Kumar, R., Chen, S., Scheurer, D., Wang, Q. L., Duh, E., Sung, C. H., Rehemtulla, A., Swaroop, A., Adler, R. and Zack, D. J. (1996). The bZIP transcription factor Nrl stimulates rhodopsin promoter activity in primary retinal cell cultures. The Journal of Biological Chemistry 271, 29612-29618. Lee, J. E. (1997). Basic helix-loop-helix genes in neural development. Current Opinions in Neurobiology 7, 13-20. Lem, J., Applebury, M. L., Falk, J. D., Flannery, J. G. and Simon M. I. (1991). Tissue-specific and developmental regulation of rod opsin chimeric genes in transgenic mice. Neuron 6, 201-210. Lim, J., Chang, J. L. and Tsai, H. J. (1997). A second type of rod opsin cDNA from the common carp (Cyprinus carpio). Biochimica et Biophysica Acta 1352, 8-12. Li, X., Chen, S., Wang, Q., Zack, D. J., Snyder, S. H. and Borjigin, J. (1998). A pineal regulatory element (PIRE) mediates transactivation by the pineal/retina-specific transcription factor CRX. Proceedings of the National Academy of Science of the United States of America 95, 1876-1881. Ma, G. C. (1999). Characterization of the regulatory elements of the common carp (Cyprinus carpio). Master thesis, National Taiwan University, Taipei, Taiwan. Morabito, M. A., Yu, X. and Barnstable, C. J. (1991). Characterization of developmentally regulated and retina-specific nuclear protein binding to a site in the upstream region of the rat opsin gene. The Journal of Biological Chemistry 266, 9667-9672. Nathans, J. (1992). Rhodopsin: structure, function and genetics. Biochemistry 31, 4923-4931. Nathans, J. and Hogness, D. S. (1983). Isolation, sequence analysis, and intron-exon arrangement of the gene encoding bovine rhodopsin. Cell 34, 807-814. Nathans, J. and Hogness, D. S. (1984). Isolation and nucleotide sequence of the gene encoding human rhodopsin. Proceedings of the National Academy of Science of the United States of America 81,4851-4855. Nickells, R. W., Burgoyne, C. F., Quigley, H. A. and Zack, D. J. (1995). Cloning and caracterization of rod opsin cDNA from the old world monkey, Macaca fascicularis. Investigative Ophthalmology & Visual Science 36, 72-82. Nie, Z., Chen, S., Kumar, R. and Zack, D. J. (1996). RER, an evolutionarily conserved sequence upstream of the rhodopsin gene, has enhancer activity. The Journal of Biological Chemistry 271, 2667-2675. Petersen-Jones, S. M., Sohal, A. K. and Sargan, D. R. (1994). Nucleotide sequence of the canine rod-opsin-encoding gene. Gene 143, 281-284. Rehemtulla, A., Warwar, R., Kumar, R., Ji, X., Zack, D. J. and Swaroop, A. (1996). The basic motif-leucine zipper transcription factor Nrl can positively regulate rhodopsin gene expression. Proceedings of the National Academy of Science of the United States of America 93,191-195. Robinson, J., Schmitt, E.A. and Dowling J. E. (1995). Temporal and spatial patterns of opsin gene expression in zebrafish (Danio rerio). Visual Neuroscience 12, 895-906. Rose, M. H., Romrell, L. J. and Kaye, G. I. (1995). In: Histology, a text and atlas, 3rd ed, Williams & Wilkins, Baltimore, USA, pp. 740-767. Saha, M. S. and Grainger, R. M. (1993). Early opsin expression in Xenopus embryos precedes photoreceptor differentiation. Molecular Brain Research 17, 307-318. Sakamoto, K., Oishi, K., Okada, T., Onuma, Y., Yokoyama, K., Sugimoto, K. and Ishida, N. (1999). Molecular cloning of the cone-rod homeobox gene (Crx) from the rat and its temporal expression pattern in the retina under a daily light-dark cycle Neuroscience Letters 261, 101-104. Sheshberadaran, H. and Takahashi, J. S. (1994). Characterization of chicken rhodopsin promoter : identification of retina-specific and glass-like protein binding domains. Molecular and Cellular Neurosciences 5, 309-318. Su,C. Y. (1998). Molecular structure of retinal photoreceptor protein gene of common carp (Cyprinus carpio). Master thesis, National Taiwan University, Taipei, Taiwan. Su, C. Y., Lim, J. and Tsai, H. J. (2000). Structural characterization and transcriptional pattern of two types of carp rhodopsin gene. Comparative Biochemistry and Physiology 125B, 37-45. Sung, C. H., Makino, C., Baylor, D. and Nathans, J. (1994). A rhodopsin gene mutation responsible for autosomal dominant retinitis pigmentosa results in a protein that is defective in localization to the photoreceptor outer segment. .Journal of Neuroscience 14, 5818-5833. Takao, M., Yasui, A. and Tokunaga, F. (1988). Isolation and sequence determination of the chicken rhodopsin gene. Vision Research 28, 471-480. Treisman, J. E., Morabito, M. A. and Barnstable, C. J. (1988). Opsin expression in the rat retina is developmentally regulated by transcriptional activation. Molecular and Cellular Biology 8, 1570-1579. Tsai, H. J., Shih, S. R., Kuo, C. M. and Li, L. K. (1994). Molecular cloning of the common carp (Cyprinus carpio) rhodopsin cDNA. Comparative Biochemistry & Physiology Part B 109, 81-88. Wilden, U., Hall, S. W. and Kuhn, H. (1986). Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments. Proceedings of the National Academy of Science of the United States of America 83, 1174-1178. Yu, X., Chung, M., Morabito, M. A. and Barnstable, C. J. (1993). Shared nuclear protein binding sites in the upstream region of the rat rod opsin gene. Biochemical and Biophysical Research Communications 191, 76-82. Yu, X., Leconte, L., Martinez, J. A. and Barnstable C. J. (1996). Ret 1, a cis-acting element of the rat opsin promoter, can direct gene expression in rod photoreceptors. Journal of Neurochemistry 67, 2494-2504. Zack, D. J,. Bennett, J., Wang. Y., Davenport, C,. Klaunberg, B., Gearhart, J. and Nathans, J. (1991). Unusual topology of bovine rhodopsin promoter-lacZ fusion gene expression in transgenic mouse retinas. Neuron 6, 187-199. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75091 | - |
dc.description.abstract | 視紫質(rhodopsin)是位於視網膜視桿細胞(rod cell)中的一種受光蛋白。目前在水生生物方面,並無視紫質基因調控機制方面的研究。為了瞭解鯉魚(Cyprinus carpio)視紫質基因其分子調控的詳細機制,利用由不同長度上游序列、以及突變某些鹼基的啟動子(promoter)來驅動水母綠螢光蛋白(EGFP)基因,並檢視其在轉殖魚的表現狀態。結果顯示,啟動子-6 kb?+74 bp,-1.2 kb?+94 bp,-138 bp?+94 bp,以及-76 bp?+94 bp的DNA片段皆能驅使轉殖基因在視網膜表現綠色螢光,而以-58 bp?+94 bp為啟動子的DNA片段則否。這代表-76 bp?+94 bp序列之中含有促使視紫質基因在視網膜表現的必要元件。在鯉魚視紫質基因上游-76 bp序列之內,包含了可能和轉錄因數Nrl及Crx結合的cis-elements。若將-76 bp?+94 bp啟動子中的-73 bp?-68 bp (putative Nrl response element)加以突變或是將其中-52 bp?-46 bp之間的序列(putative Crx binding site)移除,皆會致使此啟動子失去驅動轉殖基因在視網膜表現的能力。故此二cis-elements的共同存在對於鯉魚視紫質基因在視網膜中的表現是十分重要的。另外,注射以-6 kb?+74 bp和-1.2 kb?+94 bp為啟動子的轉殖魚,其綠色螢光在視網膜的表現率分別為62.3%及69.2%;相對之下,注射以-138 bp?+94 bp以及-76 bp?+94 bp為啟動子的轉殖魚,其表現率則分別為4.3%及3.6%。所以推測在-1.2 kb?-138 bp之間可能有強化子(enhancer)的存在。 | zh_TW |
dc.description.abstract | Rhodopsin is one of the photoreceptor proteins, which locates at rod photoreceptors in the retina. Studying on the regulatory mechanisms of rhodopsin gene in aquatics is extremely limited. To further characterize the regulatory mechanisms of carp (Cyprinus carpio) rhodopsin gene, transgenic medaka (Oryzias latipes) carrying various deletions and mutations of rhodopsin gene promoters fused with enhanced green fluorescence protein (EGFP) cDNA were examined. The results showed that promoters ranged from -6 kb to +74 bp, from -1.2 kb to +94 bp, from -138 bp to +94 bp, and from -76 bp to +94 bp were able to direct retinal expression, whereas promoter ranged from -58 bp to +94 bp was not. This suggests that upstream sequence from -76 bp to +94 bp contains elements required for retinal expression. Within the upstream -76 bp sequence, there exist putative binding sites for transcription factors Nrl and Crx. The -76 bp to +94 bp promoter with mutations in -73 bp to -68 bp sequence (putative Nrl response element) failed to drive retinal expression. The same result was obtained by removing the -52 bp to -46 bp sequence (putative Crx binding site) from the -76 bp to +94 bp promoter. Thus, both of these two cis-elements were essential for rhodopsin gene expression in retina. Moreover, the rates of green fluorescence appeared in the eyes among transgenic fish were 62.3% and 69.2% for embryos injected with -6 kb to +74 bp and -1.2 kb to +94 bp fragments, respectively. In contrast, the eye-expression rates were 4.3% and 3.6% for embryos injected with -1.2 kb to +94 bp and -138 bp tp +94 bp fragments, respectively. Therefore, there may exist enhancer(s) of rhodopsin gene within upstream sequence from -1.2 kb to -138 bp. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
dc.description.tableofcontents | Abstract……………………………………………1 Introduction………………………………………3 Materials and methods……………………………10 Carp type I rhodopsin promoter-EGFP chimeric constructs……10 Generation of transgenic medaka (Oryzias latipes)……………18 Immunohistochemistry of carp retina……………………………21 Results Expression pattern of pCMVm-injected embryos………………26 Specific expression of EGFP driven by rhodopsin gene promoter……………………………26 Retinal expression rates of embryos carrying different chimeric constructs…………27 Expression patterns of pSpM2- and pSpM3-injected embryos………………28 Expression pattern of pNCSE-76-injected embryos…………………………28 Immunohistochemical detection of Nrl and Crx in carp retina………………28 Discussion……………………………………………………………………30 References……………………………………………………………………34 Figures………………………………………………………………………39 Appendixes…………………………………………………………………46 | |
dc.language.iso | zh-TW | |
dc.title | 利用基因轉殖魚探討鯉魚(Cyprinus carpio)視紫質基因的調控機制 | zh_TW |
dc.title | Studying the Regulatory Elements of Carp (Cyprinus carpio) Rhodopsin Gene by Using Transgenic Medaka (Oryzias latipes) | en |
dc.date.schoolyear | 88-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 51 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
沒有與此文件相關的檔案。
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。