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DC 欄位 | 值 | 語言 |
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dc.contributor.author | 陳金貝 | zh_TW |
dc.date.accessioned | 2021-07-01T08:11:44Z | - |
dc.date.available | 2021-07-01T08:11:44Z | - |
dc.date.issued | 1999 | |
dc.identifier.citation | Alderdice, D.F 1988. Osmotic and ionic regulation in teleost eggs and larvae. In “Fish physiology” Vol. XIA (W.S. Hoar and D.J. Randall, eds), pp. 163-251. Academic Press, New York. Avella, M. and M. Bornancin 1989. A new analysis of ammonia and transport through the gills of the freshwater rainbow trout (Salmo gairdneri). J. Exp. Biol. 142: 155-175. Avella, M. and M. Bomancin. 1990. Ion fluxes in the gills of freshwater and seawater salmonid fish. In “Animal nutrition and transport process. 2. Transport, - respiration and excretion: Comparative and environmental aspects” (J.P. Truchot and B. Lahlou, eds), pp. 1-13. Karger, Basel. Butler, D.G. and F.J. Carmichael. 1972. (Na+-K+)-ATPase activity in eel (Anguillia rostrata) gills in relation to changes in environmental salinity: Role of adrenocortical steroids. Gen. Comp. Endocrinol. 19: 421-427. Dunel-Erb, S. and P. Laurent. 1980. Ultrastructure of marine teleost gill epithelia: SEM and TEM study of the chloride cell apical membrane. J. Morphol. 165: 175-186. Filk, G. and S.F. Perry. 1989. Cortisol stimulates whole body calcium uptake and the branchial calcium pump in freshwater rainbow trout. J. Endocrinol. 120: 75-82. Franson, M.A.H. 1985. Standard methods for the examination of water and waste water, 16th ed. American Public Health Association, Washington DC. USA. Goss, G.G., P. Laurent and S.F. Perry. 1994. Gill morphology during hypercapnia in brown bullheaad (Ictalurus nebulosus); role of chloride cells and pavement cells in acid-base regulation. 3. Fish Biol. 45: 705-718. Goss, G.G., S.F. Perry, J.N. Ferry and P. Laurent. 1998 Gill morphology and acid-base regulation in freshwater fishes. Comp. Biochem. Physiol. 119A: 107-115. Goss, G.G., S.F. Perry, C.M. Wood and P. Laurent. 1992. Mechanisms of ion and acid-base regulation at the gills of freshwater fish. J. Exp. Zool. 263: 143-159. Greco, A.M., J.C. Fenwick and S.F. Perry. 1996. The effects of soft-water acclimation on gill structure in the rainbow trout Oncorhynchus mykiss. Cell Tissue Res. 285: 75-82. Handy, R.D. 1989. The ionic composition of rainbow trout body mucus. Comp. Biochem. Physiol. 93A: 571-575. Hootman, S.R. and C.W. Philpott. 1979. Ultracytochemical localization of Na, K- activated ATPase in chloride cells from the gills of a euryhaline teleost. Anat. Rec. 193: 99-130. Hootman, S.R. and C.W. Philpott. 1980. Accessory cells in teleost branchial epithelium. Am. J. Physiol. 238: R199-R206. Hwang, P.P. 1988a. Multicellular complex of chloride calls in the gills of freshwater teleosts. J. Morphol. 196: 15-22. Hwang, P.P. 1988b. Ultrastructural study on multicellular complex of chloride cells in teleosts. Bull. Inst. Zool., Acedemia Sinica 27: 225-233. Ishihara, A. and Y. Mugiya. 1987. Ultrastructural evidence of calcium uptake by chloride cells in the gills of goldfish, Carassius auratus. J. Exp. Zool. 242: 121-129. Jobling, M. 1995. Osmotic and ionic regulation-water and salt balance. In “Environmental biology of fishes” (M. Jobling, ed.), pp. 211-249. T. J. Press (Padstow) Ltd., Padstow, Cornwall, Great Britain. Kamiya, M. 1972. Sodium-potassium-activated adenosine triphosphatase in isolated chloride cells from eel gills. Comp. Biochem. Physiol. 43B: 611-617. Karnaky, K.J., Jr. 1986. Structure and function of the chloride cell of Fundulus heteroclitus and other teleosts. Am. Zool. 26: 209-224. Karnaky, K.J., Jr. 1998. Osmotic and ionic regulation. In “The physiology of fishes” (D.H. Evans, ed.), pp. 157-176. CRC Press, New York. Keys, A.B. and E.N. Willmer. 1932. “Chloride secreting cells” in the gills of fishes, with special reference to the common eel. J. Physiol. Lond. 76: 368- 378. Krogh, A. 1938. The active absorption of ions in some freshwater animals. Z. Vergl. Physiol. 25: 335-350. Laurent, P. 1984. Gill internal morphology. In “Fish physiology Vol. XA.” (W.S. Hoar and D.J. Randall, eds.) pp. 73-184. Academic Press, Orlando. Laurent, P. and S. Dunel. 1980. Morphology of gill epithelia in fish. Am. J. Physiol. 238: R147-R159. Laurent, P. and N. Hebibi. 1989. Gill morphometry and fish osmoregulation. Can. J. Zool. 67: 3055-3063. Laurent, P. and S.F. Perry. 1990. Effects of cortisol on gill chloride cell morphology and ionic uptake in the freshwater trout, Salmo gaitdneri. Cell Tissue Res. 259: 429-442. Laurent, P. and S.F. Perry. 1991. Environmental effects on fish gill morphology. Physiol. Zool. 64: 4-25. Lee, T.H., P.P. Hwang and H.C. Lin. 1995. Mitochondria-rich cells in gills of the euryhaline teleost, Oreochromis mossambicus. Zool. Stud. 34: 239-240. Lee, T.H., P.P. Hwang, H.C. Lin. and H.L. Huang 1996. Mitochondria-rich cells in the branchial epithelium of the teleost, Oreochromis mossambicus, acclimated to various hypotonic environment. Fish Pphysiol. Biochem. 15: 513-523. Lee, T.H., J.C. Tsai, M.J. Fang, M.J. Yu and P.P. Hwang. 1998. Isoform expression of Na+-K+-ATPase α-subunit in gills of the teleost Oreochromis mossambicus. Am. J. Physiol. 275: R926-R932. Li, J., J. Eygensteyn, R.A.C., Lock, P.M. Verbost, A.J.H. van Der Heijden, S.E. Wendelaar Bonga and G. Flik. 1995. Branchial chloride cells in Larvae and juveniles of freshwater tilapia (Oreochromis mossambicus). J. Exp. Biol. 198: 2177-2184. Marshall, W.S. 1995. Transport processes in isolated teleost epithelia: Opercular epithelium and urinary bladder. In “Fish physiology Vol. XIV. lono-regulation: Cellular and molecular approaches” (C.M. Wood and T.J. Shuttleworth, eds.) pp. 1-23. Academic Press, New York. McCormick, S.D. 1990. Fluorescent labelling of Na+,K+-ATPase in intact cells by use of a fluorescent derivative of ouabain: Salinity and teleost chloride cells. Cell Tissue Res. 260:529-533. McCormick, S.D. 1995. Hormonal control of gill Na+,K+-ATPase and chloride cell function. In “Fish physiology Vol. XIV. Cellular and molecular approaches to fish ionic regulation” (C.M Wood and T.J. Shuttleworth, eds), pp. 285-3 15. Academic Press, New York. Olson, K.R. and P.O. Fromm. 1973. A scanning electron microscope study of secondary 1amellae and chloride cells of rainbow trout (Salmo gairdneri). Z. Zellforsch. Mikrook. Anat. 143: 439-449. Perry, S.F. 1997. The chloride cell: structure and function in the gills of freshwater fishes. Ann. Rev. Physiol. 59: 325-347. Perry, S.F. and G. Filk. 1988. Characterization of branchial transepithelial calcium fluxes in freshwater trout, Salmo gairdneri. Am. J. Physiol. 245: R491-R498. Perry, S.F. and G.G. Goss. 1994. The effects of experimentally altered gill chloride cell surface area on acid-base regulation in rainbow trout during metabolic alkalosis. J. Comp. Physiol. 164B: 327-336. Perry, S.F., G.G. Goss and J.C. Fenwick. 1992. The interrelationships between gill chloride cell morphology and calcium uptake in freshwater teleosts. Fish Physiol. Biochem. 10: 327-337. Perry, S.F. and P. Laurent. 1989. Adaptational responses of rainbow trout to lowed external NaCl concentration of the branchial chloride cell. J. Exp. Biol. 147: 147-168. Perry, S.F. and P. Laurent. 1993. Environmental effects on fish gill structure and function. In “Fish ecophysiology”. (J.C. Rankin and F.B. Jensen, eds.), pp. 23 1-263. Chapman and Hall, London, UK. Pickering, A.D. 1974. The distribution of mucous cells in the epidermis of the brown trout (Salmo trutta L.) and the char (Salvelinus alpinus L.). J. Fish Biol. 6:111—118. Pisam, M., B. Auperin, P. Prunet and A. Rambourg. 1993. Effects of prolactin on a and f3 chloride cells in the gill epithelium of the saltwater adapted tilapia “Oreochromis niloticus”. Anat. Rec. 235: 275-284. Pisam, M., M. Chretien, A. Rambourg and Y. Clermont. 1983. Two anatomical pathways for the renewal of surface glycoproteins in chloride cells of fish gills. Anat. Rec. 207: 3 85-397. Pisam, M. and A. Rambourg. 1991. Mitochondria-rich cells in the gill epithelium of teleost fishes: An Ultrastructurral approach. Int. Rev. Cytol. 130: 191-228. Pisam, M., C. Le Moal, B. Auperin, P. Prunet and A. Rambourg 1995. Apical structures of “mitochondria-rich”α and βcells in euryhaline fish gill: Their behaviour in various living conditions. Anat. Rec. 241: 13-24. Pisam, M. P. Prunet and A. Rambourg. 1989. Accessory cells in the gill epithelium of the freshwater rainbow trout Salmo gairdneri. Am. J. Anat. 184: 311-320. Pisam, M. and A. Rambourg. 1991. Mitochondria-rich cells in the gill epithelium of teleost fishes: An ultrastructural approach. Int. Rev. Cytol. 130:101-232. Pisam, M., C. Sardet and J. Maetz. 1980. Polysaccharidic material in chloride cell of teleostean gill: Modifications according to salinity. Am. J. Physiol. 238: R213-R218. Powell, M. D., D.J. Speare and G.M. Wright. 1994.Comparative ultranstructural morphology of lamellar epithelial, chloride and mucous cell glycocalyx of the rainbow trout (Oncorhynchus mykiss) gill. J. Fish Biol. 44:725-730. Rojo, M.C., M.J. Bl?nquez and M.E. Gonzalez. 1996. A histochemical study of the distribution of lectin binding sites in the developing branchial area of the trout Salmo trutta. J. Anat. 189: 609-621. Rosen, M.W. and N.E. Cornford. 1971. Fluid friction of fish slimes. Nature Lond. 234: 49-51. Sardet, C., M. Pisam and J. Maetz. 1979. The surface epithelium of teleostean fish gills. Cellular and junctional adaptations of the chloride cell in relation to salt adaptation. J. Cell Biol. 80: 96-117. Shikano, T. and Y. Fujio. 1998a. Immunolocalization of Na+-K+-ATPase and morphological changes in two types of chloride cells in the gill epithelium during seawater and freshwater adaptation in a euryhaline teleost, Poecilia reticulata. J. Exp. Zool. 281: 80-89. Shikano, T. and Y. Fujio. 1998b. Immunolocalization of Na+,K+-ATPase in branchial epithelium of chum salmon fry during seawater and freshwater acclimation. J. Exp. Biol. 201: 303 1-3040. Scheffey, C. and J.K. Foskett. 1982. The chloride cell: definitive identification as the salt-secretory cell in teleosts. Science. 215: 164-166. Silva, P., R. Solomon, K. Spoke, and F.H. Epistein. 1977. Ouabain inhibition of gill Na-K-ATPase: relationship to active chloride transport. J. Exp. Zool. 99:4 19-426. Spry, D.J. and C.M. Wood. 1988. Zinc influx across the isolated, perfused head preparation of the rainbow trout (Salmo gaitdneri) in hard and soft water. Can. J. Fish Aquat. Sci. 45: 2206-2215. Sullivan, G.V., J. N. Fryer and S.F. Perry. 1995. Immunolocalization of proton pump (H+-ATPase) in pavement cells of rainbow trout. J. Exp. Biol. 2619- 2629. Sullivan, G.V., J.N. Fryer and S.F. Perry. 1996. Localization of mRNA for proton pumps (H+-ATPase) and Cl-/HCO3- exchanger in rainbow trout gill. Can. J. Zool. 74: 2095-2103. Tasi, J.C. and P.P. Hwang. 1998a. The wheat germ agglutinin binding sites and development of the mitochondria-rich cells in gills of tilapia (Oreochromis mossambicus). Fish Physiol. Biochem. 19: 95-102. Tsai, J.C. and P.P. Hwang. 1998b. Effects of wheat germ agglutinin and colchicine on microtubules of the mitochondria-rich cells and Ca2 uptake in tilapia (Qreochromis mossambicus). J. Exp. Biol. 201: 2263-2271. van der Heijden, A.J.H., P.M. Verbost, J. Eygensteyn,.S.E. Wendallaar Bonga and G. Flik. 1997. Mitochondria-rich cells in gills of tilapia (Oreochromis mossambicus) adapted to freshwater or seawater: Quantification by confocal laser scanning microscopy. J. Exp. Biol. 220: 55-64. Verbost, P.M., TH.J.M. Schoenmakers, G. Flik and S.E. Wendelaar Bonga. 1994. Kinetics of ATP-and Na+ gradient driven Ca+2 transport in basolateral membranes from gills of freshwater- and seawater-adapted tilapia. 3. Exp. Biol. 196: 95-108. Wade, J.B. 1976. Membrane structural specialization of the toad urinary bladder revealed by the freeze-fracture technique. II. The mitochondria-rich cell. J. Membr. Biol. 29: 111-126. Wendelaar Bonga, S.E., G. Flik, P.H.M. balm and J.C.A. van der Meij. 1990. The ultrastucture of chloride cells in the gills of the teleost Oreochromis mossambicus during exposure to acidified water. Cell Tissue Res. 259: 575-585. Wendelaar Bonga, S.E. and J.C.A. van der Meij. 1989. Degeneration and death, by apoptosis and necrosis, of the pavement and chloride cells in the gill of teleost Oreochromis mossambicus. Cell Tissue Res. 255: 235-243. Witters, H., P. Berckmans and C. Vangenechten. 1996. Immunolocalozation of Na+,K+-ATPase in the gill epithelium of rainbow trout, Oncorhynchus mykiss. Cell Tissue Res. 283: 461-468. Zadunaisky, J.A. 1984. The chloride cell: The active transport of chloride and parcellular pathways. In “Fish physiology Vol. X B” (W.S. Hoar and D.J. randall, eds), pp. 129-174. Academic Press, New York. Zadunaisky, J.A., S. Cardona, L. Au, D.M. Roberts, E. Fisher, B. Lowenstein, E.J. Cragoe, Jr. and K.P. Spring. 1995. Chloride transport activation by plasma osmolarity during rapid adaptation to high salinity of Fundulus heteroclitus. J, Memb. Biol. 143: 207-217. 李宗翰( 1995 )吳郭魚鰓表皮 MR 細胞的型態與功能研究。台灣大學動物學研究所博士論文, 93 頁。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75079 | - |
dc.description.abstract | MR 細胞( mitochondria-rich cell )為鰓上皮細胞中,主要負責離子、滲透壓調節的細胞。在淡水環境中, MR 細胞負責鈉、氯等離子的吸收。依掃瞄式電子顯微鏡觀察其頂部表面的結構,可將 MR 細胞區分成三型:突起型(wavy-convex)、淺盆型(shallow-basin)及深洞型(Deep-hole)。本實驗為了更進一步確認三型細胞在淡水環境下的角色,首先以缺氯水(F-Cl)、高鈉水(H-Na)及高氯化鈉水(H-NaCl),確認鈉、氯離子與三型MR細胞的關係。結果顯示,在缺乏氯離子的環境中(F-Cl)主要為突起型MR細胞,若在缺乏氯離子的環境中,外加 10mM 的鈉離子時(H-Na),出現之MR細胞,仍以突起型細胞為主;另外,在環境中外加 10mM 的氯化鈉離子時( H -NaCl),則突起型MR細胞消失,深洞型MR細胞產生。故突起型細胞的產生與環境中缺乏氯離子有關而與環境中的鈉離子無關。因此推論,突起型 MR 細胞為淡水環境中主要負責氯離子吸收的細胞。此外,缺氯環境下,鰓薄板上發現的 MR 細胞,還有大量表現的多細胞組合體,增加 MR 細胞頂部表面與外界接觸的面積,加強對外界環境離子吸收的能力。進一步以植物性凝集素 ConcanavalinA(ConA)配合 Na+,K+-ATPase做雙重染色,確定 Con A 確實染在 MR 細胞頂部表面的結構上,依 Con A 的染色特性將 MR 細胞區分成突起型(wavy-convex MR cell)及非突起型(non wavy-convex MR cell)。且兩型 MR 細胞皆有Na+,K+- ATPase 的表現,皆為淡水環境下,負責滲透壓離子調節之細胞。計算兩型 MR 細胞在 F-C1 、H-Na 和 H-NaCl 中的數量表現,結果發現,F-Cl、H-Na 中,主要表現突起型細胞;在 H-NaCl 主要表現非突起型細胞,此結果與 SEM 下所觀察到的結果相似,由此可知,Con A 為標定 MR 細胞極佳的標誌。Na+ , K+-ATPase西方墨點法的實驗證實,三種低張環境下,吳郭魚鰓上皆有Na+,K+-ATPase的表現,其中又以缺氯環境下(F-Cl 和H-Na)Na+,K+-ATPase的表現量最多。此意味著 MR 細胞為了調節對不同環境中各離子的吸收,進而調整Na+,K+-ATPase的表現,以利於 MR 細胞對不同離子調節之功能。 | zh_TW |
dc.description.abstract | In gill epithelium, mitochondria-rich cells (MR cells) are considered to be the major cell type for ion-and osmo-regulation. In fresh water, gill MR cells are suggested to be responsible for ion uptake. Based on SEM (scanning electron microscope) observations on the apical surface, MR cells are classified into three types, wavy-convex, shallow-basin, and deep-hole types. Fish were acclimated to three hypotonic medium, F-Cl (chloride-free) ,H-Na (high Na2SO4) and H-NaCl (high NaCl), and the relative densities of the three types of MR cells were examined. The major cell type in chloride-free medium (F-Cl and H-Na) was wavy-convex, while that in H-NaCl was deep-hole. The major function of wavy-convex MR cells was suggested to be associated with uptake of Cl- but not Na+. Moreover, numerous multicellular complexes of MR cells developed in the chloride-free medium, suggesting to increase the apical surface area of MR cells for the enhancement of Cl- uptake. According to the results of double-labeling of Con A and Na+,K+-ATPase, the patterns of the relative abundance of MR cells labelled with Con A were similar to those examined by SEM, indicating that Con A is another suitable indicator to distinguish different types of MR cells. Western boltting analysis indicated that the amount of gill Na+,K+-ATPase was higher in the tilapia acclimated to F-Cl and H-Na than those acclimated to H-NaC1, suggesting that gill MR cells expressed various levels of Na+,K+-ATPase to perform the transport functions for different ions. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:44Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
dc.description.tableofcontents | 中文摘要. . . . . . . . . . . . . . . 1 英文摘要. . . . . . . . . . . . . . . 3 前言. . . . . . . . . . . . . . . 4 一、魚類的滲透壓調節機制. . . . . . . . . . . . . . . 4 二、鰓的形態和鰓上皮細胞. . . . . . . . . . . . . . . 5 三、 MR 細胞的形態. . . . . . . . . . . . . . . 7 四、 MR 細胞的功能. . . . . . . . . . . . . . . 10 五、研究目的. . . . . . . . . . . . . . . 13 材料與方法. . . . . . . . . . . . . . . 14 一、實驗動物. . . . . . . . . . . . . . . 14 二、研究方法. . . . . . . . . . . . . . . 14 (一)馴養環境用水的配製. . . . . . . . . . . . . . . 14 (二)水質測定. . . . . . . . . . . . . . . 15 (三)掃瞄式電子顯微鏡. . . . . . . . . . . . . . . 15 (四)西方墨點法. . . . . . . . . . . . . . . 15 (五) MR 細胞的螢光染色 . . . . . . . . . . . . . . . 17 (六) MR 細胞大小、密度的計算. . . . . . . . . . . . . . . 18 三、試驗方法步驟. . . . . . . . . . . . . . . 19 結果. . . . . . . . . . . . . . . 21 一、 MR 細胞的分佈、形態. . . . . . . . . . . . . . . 21 二、低張環境下, MR 細胞的數量、型態及分佈位置. . . . . . . . . . . . . . . 21 三、 Con A標定MR細胞的頂部表面. . . . . . . . . . . . . . . 22 四、鰓表皮上Na,K-ATPase的免疫定位. . . . . . . . . . . . . . . 24 討論. . . . . . . . . . . . . . . 27 一、突起型 MR 細胞與氯離子的調節. . . . . . . . . . . . . . . 27 二、 Con A的染色與MR細胞的關係. . . . . . . . . . . . . . . 28 三、淡水環境下,Na+,K+-ATPase的表現. . . . . . . . . . . . . . . 30 四、鰓薄板上的MR細胞及淡水多細胞組合體. . . . . . . . . . . . . . . 32 結語. . . . . . . . . . . . . . . 34 謝辭. . . . . . . . . . . . . . . 36 參考文獻. . . . . . . . . . . . . . . 38 表與圖. . . . . . . . . . . . . . . 50 | |
dc.language.iso | zh-TW | |
dc.title | 吳郭魚鰓 MR 細胞之研究:形態及運輸蛋白之表現 | zh_TW |
dc.title | Study on Gill Mitochondria - rich ( MR ) Cells of Tilapia ( Oreochromis mossambicus ) : Morphology and Transporter Expression | en |
dc.date.schoolyear | 87-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 78 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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