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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陸惠雯 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:43Z | - |
| dc.date.available | 2021-07-01T08:11:43Z | - |
| dc.date.issued | 1999 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75075 | - |
| dc.description.abstract | 在真核細胞中，hnRNP 蛋白質是一群結合到 hnRNA 上的巨大多蛋白複合體，這些 hnRNP蛋白質參與了pre-mRNA 的切割和 mRNA 的運送過程，其中 hnRNPK 與 hnRNPEl/E2是主要結合到poly-C 核酸序列的蛋白質。 急性發炎反應時，C/EBPβ是最主要調控急性反應基因表現的轉錄因數，而 hnRNP K 在 C/EBPβ調控的基因活化上是作為一負調節者，在這篇論文中，我找到另一個poly-C 結合蛋白－mCBP也可以抑制 C/EBPβ誘發的基因活化現象，並且發現登革熱病毒的核心蛋白可以與 hnRNP K 或 mCBP 產生專一性的交互作用，此外，登革熱核心蛋白可以減少 mCBP 對 C/EBPβ誘發的基因活化的抑制效果，但不會影響 hnRNP K抑制C/EBPβ之效果。 | zh_TW |
| dc.description.abstract | In eukaryotic cells, nascent RNA transcripts are associated with Large, multiprotein complex called heterogeneous nuclear ribonucleoprotein complexes (hnRNPs). These hnRNPs participate in the processing of pre-mRNA and in the export of mRNAs from the nucleus. Among these hnRNPs, hnRNP K and hnRNP E1/E2 are known to be the major poly-C binding proteins. Transcription factor C/EBPβhas been known to regulate a wide array of genes including those involved in the acute-phase response. It has been shown that hnRNP K is a negative regulator of C/EBPβ-mediated agp gene activation. In this report, I demonstrated that mCBP is another negative regulator of C/EBPβ-mediated agp gene activation. I also characterized the interaction between Dengue virus core protein and hnRNP K or mCBP. Furthermore, Dengue virus core protein can relieve the suppression effect of mCBP but not hnRNP K on the C/EBPβ-mediated agp gene activation. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:43Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
| dc.description.tableofcontents | 縮寫表............................................................1 英文摘要................................................................2 中文摘要................................................................3 第一章、緒論(Introduction) .............................................4 第二章、材料與方法(Materials and Methods) ..........10 一、質體之抽取............ .......................10 二、製備勝任細胞與細胞轉型............ ...11 二、聚合?連鎖反應得到老鼠CBP 基因.........................13 四、質體的構築. .............................................................. 15 五、表現GST 融合蛋白質..................18 六、Glutathione (GSH) 親和性管柱純化融合 蛋白質............................................18 七、SDS 聚丙醯胺膠體電泳.............19 八、蛋白質 mCBP 多源抗體的製備...................21 九、西方墨點法.......................................................22 十、細胞培養..................................23 十一、全細胞液之抽取......................................24 十二、細胞轉染 (Transfection):採用磷酸鈣與 DNA 共沈澱法...........................24 十三、氯黴素乙醯基轉換?分析...............26 十四、β-galactosidase activity..............................27 十五、固相激?活性測定法.........................27 十六、GST 蛋白質結合反應..........29 十七、免疫螢光細胞染色法.....................30 第三章、結果(Results) .........................32 第四章、討論(Discussion) .........................38 | |
| dc.language.iso | zh-TW | |
| dc.title | 多聚胞甘酸交互作用的蛋白質之研究 | zh_TW |
| dc.title | Identification and Characterization of Poly C Binding Proteins | en |
| dc.date.schoolyear | 87-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 71 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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