請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75056完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 楊欣雅 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:37Z | - |
| dc.date.available | 2021-07-01T08:11:37Z | - |
| dc.date.issued | 1999 | |
| dc.identifier.citation | Barry, S.T., and D.R. Critchley, (1994) The rho-A dependent assembly of focal adhesion in Swiss 3T3 cell is associated with increased tyrosine phosphorylation and the recruitment of both pp125FAK and protein kinase C-d to focal dhesions. J. Cell. Sci., 107:2033-2045. Bruns, R. F., (1990) Adenosine receptors: roles and pharmacology. Ann. N. Y. Acad. Sci., 603: 211-226. Burridge, K. et al., (1997) Focal adhesion assembly. Trends. Cell. Biol., 7:342-347. Chen, J. H., (1992) The possible role of protein kinase C in hemocyte aggregation and adhesion in the California mussel (Mytilus californianus). In The Cell Activation Model of Hemocyte Aggregation And Adhesion in The California Mussel (Mytilus californianus). Chap.3. pp. 91-108., PH.D Thesis. Oregon State University. Chen, J. H., and C. J. Bayne, (1995 a) Bivalve mollusc hemocyte behaviors: characterization of hemocyte aggregation and adhesion and their inhibition in the California mussel (Mytilus californianus). Biol. Bull., 188: 255-266. Chen, J. H., and C. J. Bayne, (1995 b) Hemocyte adhesion in the California mussel (Mytilus californianus) regulation by adenosine. BBA, 1268: 178-184. Chen, J. H., et al. (1996) Characterization of abalone (Haliotis diversicolor) hemocyte in vitro. Biol. Bull. NTNU., 31(1): 31-38. Daly, J. W., (1985) Adenosine receptors. Advance in Cyclic Nucleotide and Protein Phosphorylation Research., 19:29-46. Doherty, C. M., et al. (1995) Characterization of two second messenger pathways and their interactions in eliciting the human sperm acrosome reaction. J. Andro., 16:36-46. Dyrynda, E., and N. Ratcliffe, (1998) Invertebrate immune defence. The Biochemist, 1998 Feb: 6-11. Eppler, C. M., et al. (1986) Structure studies on a family of cAMP-binding protein in the nervous system of Aplysia. J. Cell. Biol., 102: 320-331. Feng, S.Y., (1988) Cellular defense mechanisms of oysters and mussels. In Disease Processes in Marine Bivalve Molluscs. edited by W. S. Fisher. pp.153-168. American Fisheries Society Special Publication 18, USA. Guan, J.-L., (1997) Focal adhesion kinase in integrin signaling. Matrix. Biol., 16:195-200. Hegde, A. E., et al. (1993) Regulatory subunits of cAMP-dependent protein kinases are degraded after conjugation to ubiquitin: a molecular mechanism underlying long-term synaptic plasticity. Proc. Natl. Acad. Sci. USA, 90: 7436-7440. Hynes, R. O., (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell., 69:11-25. Haller, H. et al., (1998) Integrin-induced protein kinase Cα and Cε translocation to focal adhesion mediates vascular smooth muscle cell spreading. Circ. Res. 82:157-165. Lewis, J.M. et al., (1996) Protein kinase C regulates avb5-dependent cytoskeletal association and focal adhesion kinase phosphorylation. J. Cell. Biol., 134(5): 1323-1332. Liliental, J. and D. D. Chang, (1998) RACK1, a receptor for activated protein kinase C interacts with integrin β subunit. J. Biol. Chem., 273(4):2379-2383. Litchfield, D.W. and E.H. Ball, (1986) Phosphorylation of cytoskeletal protein talin by protein kinase C. Biochem. Biophys. Res. Comm. 134(3): 1276-1283. Mau, S. E., et al., (1997) Cross-talk between cellular signaling pathways activated by substance P and vasoactive intestinal peptide in rat lactotroph-enriched pituitary cell cultures. Endocrinology, 138: 1704-1711. Mobley, P. L., et al., (1994) Decreased phosphorylation of four 20-Kda proteins precedes staurosporin-induced disruption of the actin/myosin cytoskeleton in rat astrocytes. Exp. Cell. Res., 214: 55-66. Newton, A. C., (1997) Regulation of protein kinase C. Curr. Opin. Cell. Biol., 9:161-167. Ogreid, D., et al. (1985) Activation of protein kinase isozymes by cyclic nucleotide analogs used singly or in combination. Eur J. Biochem., 150: 219-227. Ogreid, D., et al. (1989) Comparison of the two classes of binding sites (A and B) of type I and type II cyclic-AMP-dependent protein kinases by using cyclic nucleotide analogs. Eur J. Biochem., 181: 19-31. Olah, M.E., and G.L.Stiles, (1995) Adenosine receptor subtypes:characterization an therapeutic regulation. Annu. Rev. Pharmacol. Toxicol. 35:581-606. Palazzolo, M., et al. (1989) Mutiple cAMP-binding proteins in Aplysia tissues. J. Neurobiol., 20(8): 746-761. Panara, F., et al., (1996) Characterization and immunocytochemical localization of actin and fibronectin in haemocytes of the mussel Mytilus galloprovincialis. Histochem. J., 28:123-131. Quest, A. F. G. (1996) Regulation of protein kinase C: a tale of lipids and proteins. Enzyme Protein, 49: 231-261. Sawynok, J., et al, (1993). Caffeine as analgestic adjuvant: a review of pharmacology and mechanisms of action. Pharmacol. Rev. 45(1):43-85. Schwartz, M.A. et al., (1995) Integrins: emerging paradigms of signal transduction. Annu. Rev. Cell. Dev. Biol., 11:549-599. Spark, A. K., et al., (1988) Inflammation and wound repair in bivalve molluscs. In Disease Processes in Marine Bivalve Molluscs. edited by W. S. Fisher. pp.139-152. American Fisheries Society Special Publication 18, USA. Tasken, K., et al. (1997) Structure, function, and regulation of human cAMP-dependent protein kinases. Advances in Second Messenger and Phophoprotein Research., 31: 191-204. Taylor, S. S., et al. (1990) cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes. Annu. Rev. Biochem., 59: 971-1005. Vouri, K and E. Ruoslahti, (1993) Activation of protein kinase C precedes a5bl integrin-mediated spreading on fibronectin. J. Biol. Chem., 268:21459-21462. Watson, P.A., et al. (1994) Molecular cloning and characterization of the type VII isoform of mammalian adenylyl cyclase expressed widely in mouse tissues and in S49 mouse lymphoma cells. J. Biol. Chem., 269(46):28893-28898. Woods, A and J.R. Couchman, (1992) Protein kinase C involvement in focal adhesion formation. J. Cell. Sci., 101:277-290. Wray, W., et al., (1981). Silver staining of proteins in polyacrylamide gels. Anal. Biochem., 118:197-203. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75056 | - |
| dc.description.abstract | 由九孔（Haliotis diversicolor）血液細胞附著的實驗結果顯示，protein kinase抑制物staurosporin會抑制九孔血液細胞附著量降至70％，而PKC專一性抑制物calphostin C可顯著加強staurosporin的抑制作用，使細胞附著減少為60％，因此PKC可能參與調控九孔血液細胞的附著反應。但以caffeine抑制九孔血液細胞內的PKA活性及細胞附著，卻能藉由PMA活化PKC而抵消，由此推測PKC和PKA對於血液細胞附著反應的調控可能產生交談作用。此外，鈣離子通道A23187存在時，也會抵消caffeine對細胞附著的抑制作用，也可能存在其他Ca2＋-dependent的訊息傳遞系統參與調控細胞附著。另外，血液細胞以PKA專一性抑制物KT5720處理可顯著加強caffeine的抑制作用，由實驗結果推測除了PKC以外，PKA也參與了九孔血液細胞的附著反應。而控制細胞附著的分子位於細胞膜上，本實驗中觀察到細胞膜上35、33.5、26.5 kDa的三種蛋白質在活化PKA或PKC時，蛋白質的磷酸化程度加強；但抑制PKA或PKC活性時，蛋白質的磷酸化程度則減弱。因此推測這三種蛋白質與PKA和PKC啟動的細胞附著的反應有關。但這些細胞膜蛋白分子在九孔血液細胞附著過程中實際所扮演的角色仍有待進一步的實驗探討。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:37Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
| dc.description.tableofcontents | 一、前言……………………………………………………………………………………1 二、材料與方法…………………………………………………………………………7 1．九孔……………………………………………………………………………………7 2．化學試劑………………………………………………………………………………7 3．九孔血液細胞的附著…………………………………………………………………8 4．測量九孔血液細胞內的PKA及PKC活性………………………………………………9 5．SDS-PAGE………………………………………………………………………………9 6．蛋白質轉漬（Western blotting）…………………………………………………10 7．膠片染色：銀染法（silver staining）…………………………………………11 8．免疫轉漬（Immunoblotting）………………………………………………………11 9．細胞膜蛋白生物素標定（Biotinylation of the plasma proteins）…………11 10．Peroxidase conjugated streptavidin staining………………………………12 三、結果…………………………………………………………………………………13 1．Protein kinase對九孔血液細胞附著的影響………………………………………13 2．PKC對九孔血液細胞附著的影響……………………………………………………13 3．PKA對九孔血液細胞附著的影響……………………………………………………14 4．九孔血液細胞內PKA活性的測定……………………………………………………15 5．九孔血液細胞附著過程中細胞膜蛋白質絲胺酸磷酸化的變化……………………16 四、討論…………………………………………………………………………………18 五、參考文獻……………………………………………………………………………23 | |
| dc.language.iso | zh-TW | |
| dc.subject | 九孔血液細胞 | zh_TW |
| dc.subject | 細胞附著 | zh_TW |
| dc.subject | 訊息傳遞 | zh_TW |
| dc.title | 蛋白質激脢調控九孔（Haliotis diversicolor）血液細胞的附著反應 | zh_TW |
| dc.title | The Regulation of Hemocyte Adhesion by Protein Kinases in Abalone (Haliotis Diversicolor) | en |
| dc.date.schoolyear | 87-2 | |
| dc.description.degree | 碩士 | |
| dc.subject.keyword | 九孔血液細胞,細胞附著,訊息傳遞, | zh_TW |
| dc.relation.page | 55 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。