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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 湯智斌 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:14Z | - |
| dc.date.available | 2021-07-01T08:11:14Z | - |
| dc.date.issued | 1998 | |
| dc.identifier.citation | 天野景從 1937. ハマグリ斑紋の變異。 Venus, Ⅶ: 151-158。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74997 | - |
| dc.description.abstract | 花蛤(Gomphina venerifrmis Lamarck,1818)的傳統鑑種方式以殼色及殼紋為主,但殼色及殼紋在花蛤種內的變異相當大,常有鑑種困難的問題發生;因此本研究分別於彰化伸港、雲林臺西、福建平潭及金門等四個地區採集花蛤為材料,以外殼形質的測量、軟體部份的解剖、加上RAPD電泳分析,比較臺灣海峽兩岸的花蛤族群在形態及遺傳上的變異,並找出可供鑑種參考的形質。 研究結果發現花蛤外殼形質中的殼長與殼寬、殼長與殼高、及殼長與套線彎入長度等三種關係之間,均呈現顯著的正相關性(相關係數皆趨近於1),故殼長與這三種形質之關係或比值等應可作為鑑種的參考。統計結果顯示臺灣與福建的花蛤在殼長與上述其他三種形質的比值上皆達到顯著差異的水準,可將臺灣與福建沿海的花蛤族群區分為不同的系群(group);但是這四個採樣地點的花蛤在軟體解剖上的特徵並無任何顯著差異之處,顯然由軟體解剖無法證明臺灣海峽兩岸的花蛤是否有進一步族群分化的現象。 本研究再以RAPD技術探討花蛤的族群基因組成,發現不論以何種RAPD標幟進行分析,臺灣與福建兩地的花蛤在每一標幟的個體出現頻率上均有明顯的差異存在。在所選用的14個遺傳標幟(marker)中,有6個(42.9%)標幟呈現出臺灣產的花蛤特有的現象;而有1個標幟(7.0%)是福建產的花蛤所獨有的,顯然地,專一性(specific)的標幟共佔了近50%的比例。由主成份分析發現, U70-400、U70-1100和U45-700等三個標幟可明顯地將臺灣與福建的花蛤區分為不同系群,但臺灣的伸港與臺西的花蛤無法加以區分,同樣地福建的金門與平潭的花蛤亦無法加以區別。 將RAPD電泳資料以PAUP程式集計算個體之間對每一標幟的差異性,進一步進行UPGMA類聚分析,結果顯示地理上較為接近的花蛤群之間的遺傳變異程度很低,在枝狀圖上被歸屬於同一主軸之下;而臺灣與福建兩地的花蛤則分別歸屬於不同的分支。伸港與金門、平潭兩採樣點之間的遺傳距離(Nei's genetic distance)為0.34及0.33,臺西與金門、平潭之間則為0.35及0.34。在比較其他不同科的雙殼貝類遺傳距離後,本研究結果認為臺灣海峽兩岸所產的花蛤可能為不同的種類(species)。伸港與臺西之間的遺傳距離為0.07,金門與平潭之間則為0.08,顯示同種內不同採樣點的花蛤之間其遺傳變異程度很低,種內之間並無族群分化的現象出現。 | zh_TW |
| dc.description.abstract | Traditional species identification of short-necked clam, Gomphina veneriformis, has mainly relied on morphological characters and color-pattern of shell. However, differences within species in shell characters may be greater than among species, causing problems in species identification. In the present study, four groups of short-necked clam were collected from bath side of the Taiwan Strait. Shell characters, soft-body characters and RAPD markers variation at 7 selected primers were examined to find reliable characters for species identification. The data demonstrate that the ratios of shell length to shell width,shell length to shell height,shell length to the length of pallial sinus are important taxonomic characters. In the anatomy study, there is no significant structural differences between the four groups. However, morphological characters were unable to respond the population diversity. The population genetics was studied by using RAPD method. Using RAPD data, we were able to separate the short-necked clam collected from Taiwan and Mainland China to different species. U70-400,U70-1100 and U45-700 are important genetic markers. This result,together with morphological characters supports above conclusion.In addition, little genetic variation is found among different populations of each of the four sampling sites. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:14Z (GMT). No. of bitstreams: 0 Previous issue date: 1998 | en |
| dc.description.tableofcontents | 中文摘要.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 英文摘要Abstract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 緒論.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 材料與方法.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 結果... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 討論.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 結論. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33 參考文獻. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 謝辭.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 附圖. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43 附表.. . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 | |
| dc.language.iso | zh-TW | |
| dc.title | 臺灣海峽產花蛤(Gomphina veneriformis Lamarck,1818)分類地位暨族群判定上的研究 | zh_TW |
| dc.title | The Taxonomy and Population Analysis of Gomphina veneriformis Lamarck,1818 from Taiwan Straits | en |
| dc.date.schoolyear | 86-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 80 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位: | 漁業科學研究所 | |
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