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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林啟萬(Chii-Wann Lin) | |
| dc.contributor.author | Lequn Chen | en |
| dc.contributor.author | 陳樂群 | zh_TW |
| dc.date.accessioned | 2021-06-17T09:07:09Z | - |
| dc.date.available | 2019-12-25 | |
| dc.date.copyright | 2019-12-25 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-12-11 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74763 | - |
| dc.description.abstract | 生物感測器是近年來研究的熱門領域，表面電漿子共振應用於生物感測對生物分子進行偵測，由於實驗操作相對複雜，受限於操作人員培訓之繁瑣，無法真正普及商業用途。同時生物檢測方法不斷的發展，根據不同的檢測需求可以找到不同的檢測方法進行優化，但生物檢測往往受限於檢測時間較長且操作繁瑣，對於日益擴大的病患需求，加快檢測速度的方式顯得更為急迫。
本研究提出以共振角度位移量測的表面電漿子共振與LAMP生物檢測方法相結合以加快檢測之速率。本論文對表面電漿子共振原理進行研究應用，嘗試利用LAMP檢測核酸分子的方式與表面電漿子共振結合，提出SPR-LAMP的機構，並以此機構應用於抗甲氧西林金黃色葡萄球菌的檢測。本研究從機構設計和生物設計兩方面進行介紹，並對二者結合所作之機構改進，設計了一系列的實驗對系統性能進行驗證。本研究從機構設計和生物設計兩方面進行介紹，並對二者結合所作之機構改進，設計了一系列的實驗對系統性能進行驗證。該系統架構具有較好的穩定性，通過實驗量測系統之角度解析度為0.0154°，對應系統之偵測極限為1.23×10-4 RIU，敏感度為125°/RIU。LAMP於SPR機台之訊號可大致在15 min判定結果。針對實驗結果，對存在污染的原因與解決方式進行討論並提出了未來改進之方向。 | zh_TW |
| dc.description.abstract | Surface Plasmon Resonance (SPR) biosensor is a widely used from biological studies to clinical diagnosis application. In the situation of growing needs for real time detection, the SPR system has high potential in the early diagnosis. We proposed a SPR system based on angle shift which is more stable and easier to be used when different samples were tested. The SPR system integrated with isothermal amplification can be used in real time detection for DNA amplification.
The mechanism of SPR was discussed and the design of our system is well explained in this thesis. Also, we made an intensive theoretical study of Loop mediated isothermal amplification (LAMP) and attempted to conduct the LAMP on SPR. Finally, we adapted the SPR system to amplify the gene mecA which is the marker of Methicillin-Resistant Staphylococcus Aureus (MRSA) within an hour. The angular resolution of our system is 0.0154°, the limit of detection is 1.23×10-4 RIU and the sensitivity is 125°/RIU. The signal of the LAMP can be detected by our SPR system. According to the problems showed during the experiment, reasons were discussed, conclusions were summarized and so are the future work. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T09:07:09Z (GMT). No. of bitstreams: 1 ntu-108-R06945044-1.pdf: 14536306 bytes, checksum: 4c3201ded54e993bc86f4bd49ef0e60d (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 致 謝 I
摘 要 II ABSTRACT III 目 錄 IV 圖目錄 VI 表目錄 X 第1章 緒 論 1 1.1 研究背景 1 1.1.1 醫療領域的細菌感染 1 1.1.2 抗生素濫用與細菌耐藥性 2 1.1.3 生物感測器的發展 3 1.2 研究動機 4 1.3 研究貢獻 5 1.4 論文架構 6 第2章 基本原理與文獻回顧 7 2.1 MRSA的耐藥機制與檢測 7 2.1.1 醫療領域的細菌感染 7 2.1.2 基因組機制 7 2.1.3 MRSA的檢測方式 8 2.1.4 生物被膜 9 2.2 LAMP檢測原理和優勢與缺陷 11 2.2.1 LAMP原理 11 2.2.2 LAMP與其他DNA擴增方式比較 15 2.3 SPR生物感測技術 17 2.4 共振角度位移量測 23 2.5 SPR-LAMP 25 第3章 系統設計 26 3.1 感測器軟硬體系統設計 26 3.1.1 光學系統設計 26 3.1.2 晶片、載台與流體系統設計 31 3.1.3 加熱模組與溫度控制 35 3.2 LabVIEW程式設計 35 3.3 LAMP反應設計 37 3.3.1 DNA模板獲取 37 3.3.2 LAMP反應設計 38 3.3.3 凝膠電泳 39 第4章 量測與功能實現 41 4.1 光學系統測試 41 4.1.1 影像測試 41 4.1.2 系統穩定性、敏感度和解析度 41 4.2 Primer性能測試 44 4.3 SPR-LAMP於研究系統上的測試 45 4.4 LAMP污染問題的討論 48 4.5 LAMP污染排除 49 第5章 結論與展望 51 參考文獻 52 | |
| dc.language.iso | zh-TW | |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | 恆溫環狀擴增法 | zh_TW |
| dc.subject | 抗甲氧西林金黃色葡萄球菌 | zh_TW |
| dc.subject | 共振角度位移 | zh_TW |
| dc.subject | 表面電漿子共振 | zh_TW |
| dc.subject | 生物被膜 | zh_TW |
| dc.subject | Surface plasmon resonance | en |
| dc.subject | Angle-shift | en |
| dc.subject | Biosensor | en |
| dc.subject | Loop mediated isothermal amplification | en |
| dc.subject | Methicillin-resistant staphylococcus aureus | en |
| dc.subject | Biofilm | en |
| dc.title | 基於共振角度位移量測之表面電漿子共振生物感測器應用於抗青霉素金黃色葡萄球菌的恆溫擴增即時偵測 | zh_TW |
| dc.title | An Angle Shift-Based Surface Plasmon Resonance System for Real-Time Methicillin-Resistant Staphylococcus Aureus Detection by Loop-Mediated Isothermal Amplification | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林致廷(Chih-Ting Lin),施博仁(Po-Jen Shih) | |
| dc.subject.keyword | 表面電漿子共振,共振角度位移,生物感測器,恆溫環狀擴增法,抗甲氧西林金黃色葡萄球菌,生物被膜, | zh_TW |
| dc.subject.keyword | Surface plasmon resonance,Angle-shift,Biosensor,Loop mediated isothermal amplification,Methicillin-resistant staphylococcus aureus,Biofilm, | en |
| dc.relation.page | 55 | |
| dc.identifier.doi | 10.6342/NTU201904178 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2019-12-11 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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