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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74745完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張英?(Ing-Feng Chang) | |
| dc.contributor.author | Sih-Sian Wu | en |
| dc.contributor.author | 吳思賢 | zh_TW |
| dc.date.accessioned | 2021-06-17T09:06:51Z | - |
| dc.date.available | 2021-12-26 | |
| dc.date.copyright | 2019-12-26 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-12-20 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74745 | - |
| dc.description.abstract | 在過去研究中，LEA蛋白家族被認為參與在像是乾旱、寒害、鹽分逆境或是離層素處理等的植物逆境反應中。阿拉伯芥第四群中的lea4-1/4-2雙突變株與lea4-5 單突變株被發現在鹽逆境處理下與野生型相較有較低的萌芽率。但是是否有其他第四群LEA也有參與在鹽逆境或是離層酸反應中還不是很清楚。此外，研究室先前發現ABF3會結合ABI5的啟動子並正向調控ABI5表現。另一方面，abf3突變株的DNA晶片微陣列的實驗結果顯示LEA4-3可能是ABF3的下游目標。為了探討在阿拉伯芥中ABF3和LEA4-3之間的轉錄關係與LEA4-3在鹽逆境和離層酸影響下的功能，本研究利用qPCR發現在鹽與離層酸處理下，abf3突變株中的LEA4-3基因表現與野生型相比有較低的趨勢。在染色質免疫沉澱實驗中，發現ABF3會與LEA4-3的啟動子結合。在離層酸處理中，lea4-3突變株與野生型相比有較低的萌芽率，且LEA4-3過量表現株在離層酸處理下主根根長與野生型相比也有較長的性狀，表示lea4-3突變株相較於野生型對於離層素較敏感。而在鹽處理中lea4-3突變株有較高的萌芽率，lea4-3突變株在鹽處理中主根根長與野生型相比也有較長的性狀，表示lea4-3突變株相較於野生型對於鹽逆境較不敏感。綜合上述結果，推斷LEA4-3參與在阿拉伯芥鹽逆境與離層酸反應中。 | zh_TW |
| dc.description.abstract | Late embryogenesis abundant (LEA) proteins are known to be associated with stress responses in plants, such as dehydration, cold, high salinity, or abscisic acid (ABA) treatment. A previous study showed that group four lea mutants, lea 4-1/4-2 double mutant and lea 4-5 single mutant have lower germination rate in comparison to wild type in salt stress. LEA overexpression plants show tolerant phenotypes under many abiotic stresses. However, it is unclear whether other group four LEAs function during salt stress and ABA responses. On the other hand, our microarray data show that LEA4-3 might be a candidate target of ABF3. I aim to investigate the relationship between ABF3 and LEA4-3 in the transcriptional network, and the function of LEA4-3 in salt stress and ABA responses in Arabidopsis. In the abf3 mutant, LEA gene expression is lower than wild type under salt and ABA treatment. Chromatin immunoprecipitation (ChIP) analysis results showed that ABF3 binds to the promoter of LEA4-3. Phenotypic analysis of lea4-3 mutant lines displayed lower seed germination rate than wild type in ABA treatment, and the seed germination rate of the lea4-3 mutant line was higher than wild type in salt treatment. In addition, the primary root length of LEA4-3 overexpression lines was longer than WT in ABA and salt stress condition. Taken together, these reveal that LEA4-3 may participate in ABA and salt stress response in Arabidopsis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T09:06:51Z (GMT). No. of bitstreams: 1 ntu-108-R05B42014-1.pdf: 3203601 bytes, checksum: fd191a28c698241477d7ec8d8382a1df (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 論文口試委員會審定書 I
致謝 II 摘要 III Abbreviation V Contents VI List of figures IX Supplementary data X Appendixes XI 1. Introduction 1 1.1 Abscisic acid 1 1.2 Seed germination 1 1.3 ABA metabolism in seed germination 2 1.4 ABA signaling in seed germination 2 1.5 Environmental stresses 3 1.6 Biotic and abiotic stresses 3 1.7 Salt stress and the impact to plants 3 1.8 Transcriptional regulatory networks in abiotic stress responses 4 1.9 ABA-dependent pathway 4 1.10 ABA-independent pathway 5 1.11 Stress-responsive transcription factors in plants 5 1.12 bZIP transcription factors 6 1.13 Abscisic acid responsive elements-binding factor 3 (ABF3) 6 1.14 Late embryogenesis abundant (LEA) protein 7 1.15 LEA protein functions 7 1.16 LEA protein in seed germination 8 1.17 LEA protein in abiotic stress response 8 1.18 Project goal 9 2 Materials and Methods 11 2.1 Plant materials 11 2.2 Growth conditions of seedlings 11 2.3 Generation of constructs 12 2.4 Arabidopsis transformation by floral dipping 12 2.5 Isolation of T-DNA insertional mutants 13 2.6 RNA extraction and gene expression analysis 13 2.7 Semi-quantitative and quantitative real-time PCR analysis 14 2.8 Phenotyping of primary root length, seed germination rate and survival rate measurement 15 2.9 Chromatin immunoprecipitation (ChIP) Assays 15 3 Results 17 3.1 Transcript level of LEA4-3 in abf3 mutant affected by salt treatment 17 3.2 LEA4-3 gene expression in abf3 mutant under ABA treatment 17 3.3 Transcript level of LEA4 family in wild type, abf3 and abi5 mutants 18 3.4 Isolation of lea4-3 T-DNA insertional mutant lines 18 3.5 Isolation of ABF3 and LEA4-3 overexpression lines 19 3.6 Seed germination rate in ABA response 19 3.7 Seed germination rate in salt stress response 20 3.8 Primary root growth in ABA response 20 3.9 The primary root growth in response to salt stress 21 3.10 Survival rate comparison under salt stress condition 22 3.11 ABF3 can bind to the promoter of LEA4-3 in vivo 22 4 Discussion 23 4.1 LEA 4-3 involves in salt stress and ABA responses 23 4.2 The LEA4-3 promoter is regulated by ABF3 binding 26 References 28 Figures 35 Supplementary data 50 Appendixes 65 | |
| dc.language.iso | zh-TW | |
| dc.subject | 離層酸 | zh_TW |
| dc.subject | ABF3 | zh_TW |
| dc.subject | LEA4-3 | zh_TW |
| dc.subject | 根 | zh_TW |
| dc.subject | 種子萌芽率 | zh_TW |
| dc.subject | 鹽分逆境 | zh_TW |
| dc.subject | ABF3 | en |
| dc.subject | LEA4-3 | en |
| dc.subject | ABA | en |
| dc.subject | salt stress | en |
| dc.subject | seed germination | en |
| dc.subject | root | en |
| dc.title | 阿拉伯芥基因LEA4-3在鹽分逆境和離層酸反應之功能性研究 | zh_TW |
| dc.title | Functional study of LEA4-3 in salt stress and ABA responses in Arabidopsis thaliana | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 靳宗洛(Tsung-Luo Jinn),張孟基(Men-Chi Chang),鄭萬興(Wan-Hsing Cheng),謝明勳(Ming-Hsiun Hsieh) | |
| dc.subject.keyword | ABF3,LEA4-3,離層酸,鹽分逆境,種子萌芽率,根, | zh_TW |
| dc.subject.keyword | ABF3,LEA4-3,ABA,salt stress,seed germination,root, | en |
| dc.relation.page | 70 | |
| dc.identifier.doi | 10.6342/NTU201904410 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2019-12-23 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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