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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 葉德銘(Der-Ming Yeh) | |
dc.contributor.author | Chyun-Chien Liang | en |
dc.contributor.author | 梁群健 | zh_TW |
dc.date.accessioned | 2021-06-17T08:42:06Z | - |
dc.date.available | 2024-01-18 | |
dc.date.copyright | 2021-02-22 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2021-01-19 | |
dc.identifier.citation | 行政院農委會. 2013. 觀賞鳳梨主題館. 2 Dec 2016. <htttp://kmweb.coa.gov.tw/subject/mp.asp?mp=281>. Benzing, D.H. 2000. Bromeliaceae: Profile of an adaptive radiation. Cambridge University Press, Cambridge, U.K. Cabral, J.R.S., G. Coppens d'Eeckenbrugge, and A.P. de Matos 2000. Introduction of selfing in pineapple breeding. Acta Hort. 529:165-168. Cao, Z., S. Sui, Q. Yang, and Z. Deng. 2017. A single gene controls leaf background color in caladium (Araceae) and is tightly linked to genes for leaf main vein color, spotting and rugosity. Hort. Res. 4:1-9. Deng, Z. and B.K. Harbaugh. 2009. Leaf blotching in Caladium (Araceae) is under simple genetic control and tightly linked to vein color. HortScience 44:40-43. Deng, Z., F. Goktepe, and B.K. Harbaugh. 2008. Inheritance of leaf spots and their genetic relationships with leaf shape and vein color in caladium. J. Amer. Soc. Hort. Sci. 133:78-83. Florida Council of Bromeliad Societies. 1977. Problems with breeding and interpreting results. 10 May 2010. <http://fcbs.org/articles/interpreting.htm>. Foster, M. 1958. Neoregelia-Nidularium? A perennial question. Bromeliad Soc. Bull. 8:67-68. Foster, M. 1996. Pollination and hybridization. Florida Council Bromeliad Soc. Nwsl. 16:3-5. Griffith, L.P. 2006. Tropical foliage plants: A grower’s guide. Ball Publishing, West Chicago, IL, U.S.A. Guerra, M.P. and L.L.D.Vesco. 2010. Strategies for the micropropagation of bromeliads, p.47-66. In: S.M. Jain and S.J. Ochatt (eds.). Methods in molecular biology. Vol. 589. Protocols for in vitro propagation of ornamental plants. Humana Press, Totowa, NJ, U.S.A. Hanley, M.E., B.B. Lamont, M.M. Fairbanks, and C.M. Rafferty. 2007. Plant structural traits and their role in anti-herbivore defence. Perspectives Plant Ecol. Evol. Syst. 8:157-178. Henny, R.J. 1986. Single locus, multiallelic inheritance of foliar variegation in Aglaonema. J. Hered. 77:214-215. Heslop-Harrison, Y. and K.R. Shivanna. 1977. The receptive surface of the angiosperm stigma. Ann. Bot. 41:1233-1258. Parton, E., I. Vervaeke, R. Deroose, and M.P. De Proft. 2001. Interspecific and intergeneric fertilization barrier in Bromeliaceae. Acta Hort. 522:43-53. Soneji, J.R., P.S. Rao, and M. Mhatre. 2002. Suitability of RAPD for analyzing spined and spinless variant regenerants of pineapple (Ananas comosus L., Merr.). Plant Mol. Biol. Rpt. 20:307. (Abstract) Souza, E.H., S.M. Carmello-Guerreiro, F.V.D. Souza, M.L. Rossi, and A.P. Martinelli. 2016. Stigma structure and receptivity in Bromeliaceae. Scientia Hort. 203:118-125. Souza, F.V.D., E.H. de Souza, J.A. dos Santos-Serejo, and R.S. Cabral. 2019. BRS Anauê and BRS Boyrá: The first cultivars of ornamental pineapple developed in Brazil. Crop Breed. Appl. Biotechnol. 19:382-386. Van Tuyl, J.M., T.P. Straathof, R.J. Bino, and A.A.M. Kwakkenbos. 1988. Effect of three pollination methods on embryo development and seedset in intra- and interspecific crosses between seven Lilium species. Sex. Plant Reprod. 1:119-123. Vervaeke, I., E. Parton, L. Maene, R. Deroose, and M.P. De Proft. 2001. Prefertilization barriers between different Bromeliaceae. Euphytica 118:91-97. Vervaeke, I., E. Parton, L. Maene, R. Deroose, and M.P. De Proft. 2002. Pollen tube growth and fertilization after different in vitro pollination techniques of Aechmea fasciata. Euphytica 124:75-83. Vervaeke, I., J. Wouters, L. Stichelbout, E. Londers, R. Deroose, and M.P. De Proft. 2003. Inheritance of spineless leaves in Aechmea (Bromeliaceae). Euphytica 134:47-49. Yeh, D.M., W.J. Yang, F.C. Chang, M.C. Chung, W.L.Chen, and H.W. Huang. 2007. Breeding and micropropagation of Aglaonema. Acta Hort. 755:93-98. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74549 | - |
dc.description.abstract | 彩葉鳳梨(Neoregelia)是戶外景觀及室內觀賞的重要植物,葉色多變且低維護管理,新品種有其市場潛力;然目前對彩葉鳳梨授粉、雜交障礙等文獻有限。本研究觀察彩葉鳳梨花朵器官,調查授粉結實特性,探討雜交障礙,與應用切除花柱處理克服雜交障礙,取得雜交後代並調查其性狀,進而瞭解葉斑及葉緣小刺性狀之遺傳,以期有助育種進行。 彩葉鳳梨之花序自葉杯中抽出,總梗粗短,白色、紫色或薰衣草色小花著生於花序末端上形成短總狀花序。雌蕊為三枚心皮合生,螺旋狀柱頭。彩葉鳳梨花朵壽命不長,晴日約於0700 HR開花,1250 HR過後花瓣開始萎凋,1600 HR花瓣已向內捲曲萎縮。取彩葉鳳梨‘Gold Fever’為母本與‘Pink Sensation’為父本,於0700-0950 HR、1000-1250 HR、1300-1550 HR、1600-1800 HR四個時段雜交授粉,結果顯示僅於中午前授粉才能獲得雜交種子。另取‘Gold Fever’及‘Little Rose’為母本,與多個品種進行雜交授粉,應證上午與下午授粉之差異,仍顯示以中午前授粉為宜。彩葉鳳梨授粉時間短之特性與柱頭可授性相關,於花開前一天、開花當日不同時段及開花後一天取彩葉鳳梨‘Gold Fever’雌蕊,於柱頭滴加一滴0.88 M (約3%)過氧化氫,觀察3分鐘內反應,結果顯示開花前一天至開花當天0400-0650 HR皆無反應或極微弱,開花當天0700-1250 HR期間則有明顯氣泡反應,開花當天1300-1550 HR亦有氣泡反應,但已減弱,花後一天者仍有微弱氣泡反應。另於花開前一天、花開當天及花後一天取數個彩葉鳳梨品種之花粉培養於25℃、黑暗下,以含10%蔗糖之MBK培養基培養12小時,結果顯示彩葉鳳梨品種花粉萌芽率是否隨開花日程而變化有品種差異,部分品種在開花後一天仍有一定花粉萌芽率。 取12個彩葉鳳梨品種行自花授粉、同株異花或營養繁殖系異花自交授粉,皆無法產生種子。觀察參試7個彩葉鳳梨品種之雌蕊,依其長度可大致分為兩群,長花柱品種之花柱長度介於2.6-3.0 cm,而短花柱品種之花柱長度介於1.9-2.0 cm。取參試7個彩葉鳳梨品種於3年自然花期間,陸續進行長花柱品種間、短花柱品種間、短花柱為母本與長花柱為父本、長花柱為母本與短花柱為父本等共19個雜交組合(含6個正反交),以短花柱品種為母本,較易獲得雜交種子,而長花柱品種為母本則無法產生種子。 將自交、短花柱品種間雜交、短花柱品種為母本與長花柱品種為父本、長花柱品種為母本與短花柱品種為父本、長花柱品種間雜交等組合之雌蕊於授粉後1至5天取下,以螢光染色法觀察花粉萌發與花粉管生長。結果顯示自交授粉後,花粉雖於柱頭表面萌發,但花粉管無法進入柱頭組織。親和的雜交組合中(短花柱×短花柱、短花柱×長花柱),花粉管約於授粉後3至5天進入胚珠、完成受精。不親和的雜交組合中(長花柱×短花柱),花粉管僅能生長至花柱中段或2/3處,即發生先端膨大或生長停止等異常,導致無法受精。長花柱品種間雜交,雖可見花粉管進入胚珠,但並未獲得種子,可能有受精後障礙。取長花柱‘Meyendorffii’、‘Perfecta Tricolor’、‘Skotak’s Orange Crush’為母本,以‘Gold Fever’為父本,進行去除一半長度花柱再授粉,並以未切除花柱為對照組。去除花柱再授粉可以克服長花柱品種為母本之雜交障礙並取得種子。螢光染色觀察顯示授粉後4天可見花粉管進入胚珠、已完成受精。 取葉片有斑塊及無斑塊之彩葉鳳梨品種進行雜交,後代於栽培4-6年後成熟,表現出穩定葉色性狀。彩葉鳳梨葉片斑塊性狀由單一基因座控制,參試之有斑塊品種為異質結合顯性(Bb),無斑塊品種為同質結合隱性(bb) (χ2 = 0.18-3.00)。因無刺‘Deroose’s Medusa’為長花柱品種,故取葉緣有刺品種為母本與之雜交。結果顯示葉緣無刺性狀相對於葉緣有刺性狀為顯性遺傳。葉緣無刺後代生長緩慢且易早夭。 | zh_TW |
dc.description.abstract | Neoregelia has been widely used for outdoor landscaping and indoor decoration for their leaf color variegation and low maintenance. Developing new cultivar are required for market demands. However, there has been limited information regarding pollination habits and hybridization barriers is presently limited. This study aimed to 1) observe floral organ, 2) test pollination habits, 3) understand reporductive barriers, 4) overcome hybridization barrier through cut-style pollination, 5) obtain hybrid progenies, and 6) study inheritance of leaf blotching and spinulose. The knowledge would assist breeding of new Neoregelia cultivars. Neoregelia cultivars have terminal inflorescences with a thick and short peduncle emerge from the leaf tank. Inflorescence, a short raceme with many small white, violet or lavender flowers appear in the socket centre of the rosetted leaves that are placed at the same level and almost hidden at the bottom of the cavity that usually bloom in the water stored there. Pistil is composed of three carpels and has conduplicate-spiral dry stigma. Neoregelias have rather short anthesis time frame. Anthesis starts at around 0700 HR, corolla lobes begin to wilt at around 1250 HR, and completely wilted at around 1600 HR under sunny conditions. Neoregelia ‘Gold Fever’ was used as female parent and crossed with ‘Pink Sensation’ as male parentand pollinations were made at 0700-0950 HR, 1000-1250 HR, 1300-1550 HR, and 1600-1800 HR. Results showed that pollination should be made before noon to obtain hybrid seeds. Further confirmation was made using ‘Gold Fever’ and ‘Little Rose’ as female parents, and crossed with other cultivars. Subsequent results support that cross-pollination should be made before noon. The short pollination time of Neoregelia is related to the stigma receptivity. Stigma receptivity was tested using hydrogen peroxide (H2O2) method in order to elucidate cause of short pollination time frame. A single drop of 0.88 M (3%) H2O2 was applied to the stigma of pistil collected at one day before anthesis, various time at the day of anthesis, and one day after anthesis, and then observed for three mins. Little on no response to H2O2 was observed one day before anthesis to 0400-0650 HR at antehsis, While significant response was observed at anthehsis (0700-1250 HR), then gradually decreased at 1300-1550 HR. and weak response at one day after anthesis. Pollen of several cultivars were collected at one day before anthesis, the day of anthesis, and one day after anthesis. The collected pollen was incubated at 25oC, in the dark, using modify BK medium containing 10% sugar for 12 h. Pollen germination percentage differs among cultivars and time of harvest, however, some cultivars did germinate even at one day after anthesis. Twelve Neoregelia cultivars were received autogamous, geitonogamous, and vegetative-geitonogamous self-pollination, and results showed none produced seeds. Pistil lengths were observed in seven tested Neoregelia cultivars, and could be divided into two groups based on style length, one with relative long style lengths (2.6-3.0 cm), and another with relative short style lengths (1.9-2.0 cm). These seven cultivars were cross-pollinated when naturally-flowered to form 1) crosses between long-style cultivars, 2) crosses between short-style cultivars, 3) short-style (♀) × long-style (♂), and 4) long-style (♀) × short style (♂) across three years and a total of 19 cross combinations. Hybrid seeds were obtained only when short-style cultivars were used as female parent. Pistils of self-pollination and cross combinations between cultivars with different style lengths were collected after pollination and observed for in vivo pollen tube growth using fluorescence staining. Results showed that pollen germinated but could not grow into stigma tissue after self-pollination. In contrast, pollen tube grew into ovule and fertilization took place in compatible crosses (short-style × short-style, short-style × long-style) at 3 to 5 days after pollination. Nevertheless, pollen tube could grow into style until half to two-thirds length of the style where abnormal pollen tube tip occurred in the incompatible cross (long-style × short-style). Cross between long-styled cultivars showed that pollen tube did grow into ovule but no seed could be harvested, indicating a post-ferilization barrier. Half the length of the long-style ‘Meyendorffii’, ‘Perfecta Tricolor’, ‘Skotak’s Orange Crush’ were removed using sharp scissors and subsequently pollinated with short-style ‘Gold Fever’, while style of control remain intact. Cut-style pollination could overcome hybridization barrier of long-style cultivars and obtain hybrid seeds. Corresponding in vivo observation also showed that pollen tube grew into ovules and fertilization took place at four days after pollination. Crosses between Neoregelia cultivars with and without blotching were made. These progenies took 4-6 years to reach maturity with stable leaf variegation. Results show that leaf blotching in Neoregelia was governed by single locus, cultivars with blotching were heterozygous dominant (Bb), and non-blotching cultivars were homozygous recessive (bb) (χ2 = 0.18-3.00). Since non-spinulose ‘Deroose’s Medusa’ has long style, unilateral crosses between spinulose and non-spinulose cultivars were made. Results show that entire margin was dominant to spinulose margin. Progenies with entire margins grew slowly and tended to die when young. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:42:06Z (GMT). No. of bitstreams: 1 U0001-1801202113484500.pdf: 5839545 bytes, checksum: cbf121b5a716eb967a440821e02f3e54 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 目錄 表目錄……………………………………………………………………………………ii 圖目錄…………………………………………………………………………………iii 中文摘要…………………………………………………………………………………vi 英文摘要………………………………………………………………………………viii 第一章 前言………………………………………………………………………1 第二章 前人研究……………………………………………………………………6 一、觀賞鳳梨產業概況與分類、習性………………………………………6 二、彩葉鳳梨屬之簡介…………………………………………………………8 三、影響觀賞鳳梨開花因子………………………………………………………9 四、觀賞鳳梨授粉時間………………………………………………………….10 五、生殖系統…………….…… ………………………………………………10 六、觀葉植物葉斑性狀之遺傳…………………………………………………14 七、植物硬刺性狀之發生與遺傳………………………………………………18 第三章 彩葉鳳梨花器與授粉時間……………………………………………61 第四章 彩葉鳳梨切花柱授粉、受精與結實特性………………………………67 第五章 彩葉鳳梨雜交後代斑塊及葉緣小刺性狀之遺傳…………………………111 第六章 綜合討論及結論…………………………………………………………139 附錄……………………………………………………………………………………148 表 目 錄 表3.1. 授粉時間對彩葉鳳梨雜交種子數及雜交種子發芽率之影響………………56 表3.2.於不同開花階段以3%雙氧水檢測彩葉鳳梨‘Gold Fever’與[‘Gold Fever’ × ‘Pink Sensation’]柱頭可授性.………………………59 表3.3. 取樣時間對彩葉鳳梨花粉培養於含10%蔗糖BK培養基中、25 ℃下萌芽率變化之影響………………………………………………………62 表 4.1. 參試彩葉鳳梨品種親緣資料…………………………………………………88 表4.2. 彩葉鳳梨品種自花授粉、同株異花及營養繁殖異株授粉之種子數……89 表4.3. 彩葉鳳梨不同花柱長度親本之雜交組合所得種子數……………………91 表4.4. 長花柱及短花柱彩葉鳳梨雜交之種子數……………………………………92 表4.5. 不同花柱長度親本之正反交所得種子數……………………………………93 表4.6. 切除50%花柱處理對三個彩葉鳳梨雜交組合產生種子數之影響…………94 表5.1. 參試八個彩葉鳳梨品種親緣背景資料及葉片斑塊、葉緣有無小刺性狀…136 表5.2. 卡方檢定彩葉鳳梨品種雜交後代不規則斑塊性狀之遺傳模式…………137 表5.3. 卡方檢定彩葉鳳梨品種雜交後代葉緣性狀之遺傳模式…………………138 圖 目 錄 圖2.1. 臺灣常見觀賞鳳梨屬別簡介……………………………………………59-60 圖3.1. 彩葉鳳梨雜交授粉流程….……………………………………………………52 圖3.2. 彩葉鳳梨花序之組成………….………………………………………………53 圖3.3. 彩葉鳳梨‘Meyendorffii’之雌蕊構造…..……………………………….…54 圖3.4. 彩葉鳳梨[‘Gold Fever’ בPink Sensation’]於晴日下一日內花朵開放之變化……………………………………………………………………………55 圖3.5. 彩葉鳳梨[‘Gold Fever’ × ‘Pink Sensation’]開花前一天(A)、開花當天(B)及花後一天(C)之花器變化..………………………………………………...57 圖3.6. 以3%雙氧水檢測彩葉鳳梨[‘Gold Fever’ × ‘Pink Sensation’]開花前一天至花後一天柱頭可授性……………………………………………………58 圖3.7. 三品種彩葉鳳梨花粉於含10%蔗糖B K培養基中、25℃下萌芽率之變化………………………………………………………………………60 圖3.8. 彩葉鳳梨‘Barbarian’於含10%蔗糖BK培養基中、25℃下,培養(A) 2小時、(B) 4小時、(C) 6小時、(D) 8小時後花粉萌芽情形……………………61 圖4.1. 彩葉鳳梨長花柱‘Meyendorffii’ (左)及短花柱‘Gold Fever’ (右)雌蕊外觀……90 圖4.2. 自交授粉後6小時,彩葉鳳梨‘Barbarian’花粉萌發情形..…………………95 圖4.3. 以短花柱彩葉鳳梨‘Barbarian’為母本與短花柱‘Gold Fever’為父本雜交授粉1天後,‘Gold Fever’花粉於‘Barbarian’柱頭萌發………………96 圖4.4. 以短花柱彩葉鳳梨‘Barbarian’為母本與短花柱‘Gold Fever’為父本雜交授粉2天後,‘Gold Fever’花粉管於‘Barbarian’花柱內生長……………97 圖4.5. 以短花柱彩葉鳳梨‘Barbarian’為母本與短花柱‘Gold Fever’為父本雜交授粉3天後,‘Gold Fever’花粉管於‘Barbarian’子房內生長…………………98 圖4.6. 以短花柱彩葉鳳梨‘Barbarian’為母本與短花柱‘Gold Fever’為父本雜交授粉5天後,‘Gold Fever’花粉管由珠孔進入‘Barbarian’胚珠……………99 圖4.7. 以短花柱彩葉鳳梨‘Gespacho’為母本與長花柱‘Deroose’s Medusa’為父本雜交授粉6小時後,‘Deroose’s Medusa’花粉管於‘Gespacho’花柱內生長………………………………………………………………………………100 圖4.8. 以短花柱彩葉鳳梨‘Gespacho’為母本與長花柱‘Deroose’s Medusa’為父本雜交授粉1天後,‘Deroose’s Medusa’花粉管於‘Gespacho’花柱及子房內生長………………………………………………………………………101 圖4.9. 以短花柱彩葉鳳梨‘Gespacho’為母本與長花柱‘Deroose’s Medusa’為父本雜交授粉3天後……………………………………………………102 圖4.10. 以短花柱彩葉鳳梨‘Gespacho’為母本與長花柱‘Deroose’s Medusa’為父本雜交授粉7天後………………………………………………………103 圖4.11. 以長花柱彩葉鳳梨‘Perfecta Tricolor’為母本與長花柱‘Skotak’s Orange Crush’為父本,行去花柱授粉4天後,‘Skotak’s Orange Crush’花粉管進入子房,並進入胚珠完成授精………………………………………………104 圖4.12. 以長花柱彩葉鳳梨‘Skotak’s Orange Crush’為母本與短花柱‘Gespacho’為父本雜交授粉5天後,‘Gespacho’花粉管於花柱中、下段生長受阻………105 圖4.13. 以長花柱彩葉鳳梨‘Perfecta Tricolor’為母本與短花柱‘Gold Fever’為父本雜交授粉4天後,‘Gold Fever’花粉管生長受阻……………………106 圖4.14. 以長花柱彩葉鳳梨‘Perfecta Tricolor’為母本與短花柱‘Gold Fever’為父本,行去花柱授粉4天後,‘Gold Fever’花粉於‘Perfecta Tricolor’花柱切口萌發及於花柱內生長……………………………………………………107 圖4.15. 以長花柱彩葉鳳梨‘Perfecta Tricolor’為母本與短花柱‘Gold Fever’為父本,行去花柱授粉4天後,‘Gold Fever’花粉管於‘Perfecta Tricolor’子房內生長……………………………………………………………………………108 圖4.16. 以長花柱彩葉鳳梨‘Skotak’s Orange Crush’為母本與短花柱‘Gold Fever’為父本,行去花柱授粉4天後,‘Gold Fever’花粉於‘Perfecta Tricolor’花柱切口萌發及於花柱內生長…………………………………………………109 圖4.17. 以長花柱彩葉鳳梨‘Skotak’s Orange Crush’為母本與短花柱‘Gold Fever’為父本,行去花柱授粉4天後,‘Gold Fever’花粉管進入‘Skotak’s Orange Crush’子房,並進入胚珠完成授精…………………………………………110 圖5.1. 彩葉鳳梨雜交育種具斑塊、無斑塊與葉緣無刺品種…………126-127 圖5.2. 彩葉鳳梨雜交種子實生育苗………………………………………128-129 圖5.3. 彩葉鳳梨‘Gold Fever’與‘Gespacho’雜交(組合1)所得後代葉斑性狀表現………………………………………………………………………130 圖5.4. 彩葉鳳梨‘Gold Fever’與‘Pink Sensation’雜交(組合3)所得後代葉斑性狀表現………………………………………………………………………131 圖5.5. 彩葉鳳梨‘Gold Fever’與‘Perfecta Tricolor’雜交(組合7)所得後代葉斑性狀表現……………………………………………………………………………132 圖5.6. 彩葉鳳梨‘Gespacho’與‘Deroose’s Medusa’雜交(組合9)所得後代葉片斑塊性狀及葉緣性狀表現……………………………………………………133 圖5.7. 彩葉鳳梨‘Gespacho’ (葉緣有小刺)與葉緣無刺‘Deroose’s Medusa’雜交所得後代株型表現………………………………………………………134-135 | |
dc.language.iso | zh-TW | |
dc.title | 彩葉鳳梨之授粉行為與生殖障礙 | zh_TW |
dc.title | Pollination behavior and reproductive barrier of Neoregelia | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 沈榮壽(Rong-Show Shen),陳彥銘(Yan-Ming Chen),張耀乾(Yao-Chien Chang),許富鈞(Fu-Chiun Hsu) | |
dc.subject.keyword | 育種,過氧化氫,花粉-雌蕊交互作用,自交不親和,柱頭可授性, | zh_TW |
dc.subject.keyword | breeding,hydrogen peroxide,pollen-pistil interaction,self-incompatibility,stigma receptivity, | en |
dc.relation.page | 152 | |
dc.identifier.doi | 10.6342/NTU202100084 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2021-01-20 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
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