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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳穎練(Ying-Lien Chen) | |
| dc.contributor.author | Yu-Hsiang Yu | en |
| dc.contributor.author | 游宇祥 | zh_TW |
| dc.date.accessioned | 2021-06-17T08:35:08Z | - |
| dc.date.available | 2026-01-21 | |
| dc.date.copyright | 2021-03-11 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-01-21 | |
| dc.identifier.citation | Afroz, T., Lee, H. S., Jeon, Y. A., Sung, J. S., Rhee, J. H., Assefa, A. D., Noh, J., Hwang, A., Hur, O. S. and Ro, N. Y. 2019. Evaluation of different inoculation methods for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm. J. Crop Sci. Biotechnol. 22(2):177-183. doi:10.1007/s12892-019-0115-0 Al-Fadhal, F. A., Al-Abedy, A. N. and Alkhafije, D. A. 2019. Isolation and molecular identification of Rhizoctonia solani and Fusarium solani isolated from cucumber (Cucumis sativus L.) and their control feasibility by Pseudomonas fluorescens and Bacillus subtilis. Egypt J. Biol. Pest Co. 29(1):47. doi:10.1186/s41938-019-0145-5 Ales, L. and Lenka, S. 1997. Variation in response of several wild Pisum spp. to Fusarium solani and Fusarium oxysporum. Cereal Res. Commun. 25(3):845-846. doi:10.1007/BF03543870 Alshoosh, W. G. A. 1997. Chemical composition of some roselle (Hibiscus sabdariffa) genotypes. Master Thesis. University of Khartoum. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74426 | - |
| dc.description.abstract | 在台灣，近年之洛神葵田間病害調查報導，洛神葵萎凋病普遍發生，但其肇因仍未知。洛神葵萎凋病之病株莖基部呈現褐化且輕微縊縮之病徵，並可觀察到白色之氣生菌絲，縱切面維管束偶有褐化現象，多數則呈髓部腐爛之徵狀，鏡檢後亦可發現典型的鐮孢菌(Fusarium spp.)之大、小分生孢子，顯示此病害可能由鐮孢菌造成。本研究蒐集了二批來自台東縣六個不同種植地的洛神葵萎凋病樣本，經由ITS及16S rDNA之序列分析後，分離得之64株菌株包含真菌、卵菌以及細菌。利用柯霍氏法則測試這些分離到的菌株後發現，Fusarium solani K2 (FsK2)菌株會造成洛神葵植株萎凋及髓部腐爛且與田間相似之病徵。進一步利用掃描式電子顯微鏡觀察，於接種後發病之植株，FsK2之菌絲會纏據於莖基部表面，且偶可在腐爛的髓部觀察到小分生孢子與似侵入之構造。此研究為首次證明在台灣，茄鐮孢菌可造成洛神葵萎凋病的發生。此外，洛神葵種子帶菌檢測結果顯示沒有由種子攜帶F. solani的情形，此病害由種子傳播之機率較低。再者，我們發現Bacillus amyloliquefaciens Ba01、Streptomyces misionensis KHY26和殺菌劑百克敏(pyraclostrobin)可以防治洛神葵萎凋病，在盆栽試驗中分別達到76.9%、96.1%和96.1%的防治率，此方面可作為後續生物防治與化學防治之策略。 | zh_TW |
| dc.description.abstract | A roselle (Hibiscus sabdariffa L.) disease survey conducted recently in Taiwan reported that roselle wilt disease occurs widely; however, the causal agent is unknown. The stems of wilted roselle were browned, slightly constricted, and showed white aerial hyphae. Rotted pith was found in the vertically dissected stem base and macroconidia and microconidia typical of the Fusarium species were observed under a microscope indicating that roselle wilt might be caused by Fusarium spp. In this study, we isolated 64 strains from wilted plants grown in six roselle fields in Taitung county, Taiwan. After ITS and 16S rDNA sequence analysis, the 64 strains were identified as different species of fungi, oomycetes or bacteria. Koch’s postulates were used to evaluate the pathogenicity of these strains, among which we found that Fusarium solani K2 (FsK2) can cause wilting and rotted pith on roselles similar to those observed in the fields. Using a scanning electron microscope, we found that mycelia of FsK2 can colonize on roots, while microconidia and penetration-like structures were sometimes found in the rot pith. This is the first demonstration that F. solani can cause roselle wilt in Taiwan. On the other hand, roselle seeds were tested and no seeds were contaminated by F. solani, indicating that the disease may not be seed-borne. Furthermore, we found that Bacillus amyloliquefaciens Ba01, Streptomyces misionensis KHY26, and fungicide pyraclostrobin can control roselle wilt with a 76.9%, 96.1%, and 96.1% control rate, respectively, and therefore might serve as potential biological and chemical control strategies against roselle wilt disease in the future. | en |
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| dc.description.tableofcontents | 國立臺灣大學碩士學位論文口試委員會審定書 i 致謝 ii 中文摘要 iii Abstract iv 目錄 vi 表目錄 ix 圖目錄 x 1. Introduction 1 2. Materials and methods 6 2.1 Strains, media, and growth conditions 6 2.2 Sample collection and pathogen isolation 6 2.3 Pathogen identification and phylogenetic analysis 7 2.4 Inoculum preparation 8 2.5 Koch’s postulate and disease severity 9 2.6 Chlamydospore induction 11 2.7 Scanning electron microscopy 12 2.8 Roselle seed-borne microbial test 12 2.9 Screening of biocontrol strains for combating roselle wilt fungus 13 2.10 Screening of fungicides for combating roselle wilt fungus 14 2.11 Pot assays 15 3. Results 17 3.1 Isolation of possible roselle wilt pathogens 17 3.2 F. solani can cause the roselle wilt 17 3.3 Phylogenetic analysis and morphology of FsK2 18 3.4 Observation of roselle wilt samples with scanning electron microscope 19 3.5 F. solani is not likely transmitted by roselle seeds 20 3.6 Biocontrol agents B. amyloliquefaciens Ba01 and S. misionensis KHY26 can inhibit the growth of FsK2 21 3.7 Pyraclostrobin possessed inhibitory effects against FsK2 21 3.8 B. amyloliquefaciens Ba01 and S. misionensis KHY26 can reduce the disease severity of roselle wilt 22 3.9 Pyraclostrobin can control the roselle wilt 23 4. Discussion 24 5. Tables 29 6. Figures 35 7. Supplementary information 51 Table 51 Figures 57 8. References 62 9. Appendix 71 表目錄 Table 1. Strains isolated from diseased roselle samples in Taitung, Taiwan 29 Table 2. Biocontrol strains used in this study 31 Table 3. Primers used in this study 32 Table 4. Fungicides used in this study 33 Table 5. Disease incidence and disease severity index of roselle wilt at 49 days post inoculation 34 Table S1. Strains isolated from roselle seeds 51 圖目錄 Figure 1. Roselle wilt disease in the fields and symptoms found in Taitung, Taiwan. 35 Figure 2. Koch’s postulate test of F. solani K2 and mixed inoculation test with P. nicotianae CWR-42 at 49 days post inoculation. 36 Figure 3. Phylogenetic analysis of F. solani K2. 38 Figure 4. Morphology of the causal agent of roselle wilt, FsK2. 40 Figure 5. Observation of roselle wilt samples with scanning electron microscope. 42 Figure 6. F. solani is not likely transmitted by roselle seeds. 44 Figure 7. B. amyloliquefaciens Ba01 and S. misionensis KHY26 showed marked anti-FsK2 effect. 45 Figure 8. In vitro screening of fungicides against FsK2. 47 Figure 9. Biological and chemical control of the roselle wilt. 49 Figure S1. Disease severity index of roselle wilt disease. 57 Figure S2. F. oxysporum caused leaf yellowing symptom on roselles. 58 Figure S3. P. nicotianae can cause leaf yellowing and root rot of roselle seedlings at 25℃ with high humidity. 59 Figure S4. F. solani K1 (FsK1) can also cause roselle wilt. 60 Figure S5. B. amyloliquefaciens Ba01 can enhance the elongation of roselle roots. 61 | |
| dc.language.iso | en | |
| dc.subject | 液化澱粉芽孢桿菌 | zh_TW |
| dc.subject | 茄鐮孢菌 | zh_TW |
| dc.subject | 生物防治 | zh_TW |
| dc.subject | 洛神葵萎凋病 | zh_TW |
| dc.subject | 化學防治 | zh_TW |
| dc.subject | 百克敏 | zh_TW |
| dc.subject | 米修鏈黴菌 | zh_TW |
| dc.subject | chemical control | en |
| dc.subject | Fusarium solani | en |
| dc.subject | Bacillus amyloliquefaciens | en |
| dc.subject | Streptomyces misionensis | en |
| dc.subject | pyraclostrobin | en |
| dc.subject | biological control | en |
| dc.subject | roselle wilt disease | en |
| dc.title | 洛神葵萎凋病之鑑定與防治策略開發 | zh_TW |
| dc.title | Identification of roselle wilt disease and development of control strategies | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 109-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.advisor-orcid | 陳穎練(0000-0002-1966-470X) | |
| dc.contributor.oralexamcommittee | 鍾嘉綾(Chia-Lin Chung),林乃君(Nai-Chun Lin),陳啟予(Chi-Yu Chen),曾敏南(Min-Nan Tseng) | |
| dc.contributor.oralexamcommittee-orcid | 鍾嘉綾(0000-0002-1612-0109),林乃君(0000-0002-6485-9776) | |
| dc.subject.keyword | 洛神葵萎凋病,茄鐮孢菌,液化澱粉芽孢桿菌,米修鏈黴菌,百克敏,生物防治,化學防治, | zh_TW |
| dc.subject.keyword | roselle wilt disease,Fusarium solani,Bacillus amyloliquefaciens,Streptomyces misionensis,pyraclostrobin,biological control,chemical control, | en |
| dc.relation.page | 106 | |
| dc.identifier.doi | 10.6342/NTU202100109 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2021-01-21 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| 顯示於系所單位： | 植物病理與微生物學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-2101202110303200.pdf 未授權公開取用 | 5.5 MB | Adobe PDF |
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