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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐善慧(Shan-hui Hsu) | |
dc.contributor.author | Chih-Yu Fu | en |
dc.contributor.author | 傅智煜 | zh_TW |
dc.date.accessioned | 2021-06-17T08:29:58Z | - |
dc.date.available | 2021-04-07 | |
dc.date.copyright | 2021-04-07 | |
dc.date.issued | 2021 | |
dc.date.submitted | 2021-03-16 | |
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S.; Hsu, S. h., Fractal Structure of Hydrogels Modulates Stem Cell Behavior. ACS Macro Letters 2015, 4 (9), 1056-1061. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74326 | - |
dc.description.abstract | 冷凍凝膠是在單體或聚合物的冷凍溶液中形成的凝膠基質,其具有相互連通的大孔、結構穩定性和可壓縮性等的優點。同時,熱誘導形狀記憶材料在生醫領域中有著相當大的潛力,因為形狀記憶行為可以使材料被固定於小形狀並藉由微創手術植入欲修補之位置,最後通過外部刺激使材料恢復其初始之大形狀。但是,到目前為止沒有文獻報導關於形狀記憶冷凍凝膠的研究。本研究合成了具有形狀記憶特性的水基可生物降解的雙官能聚胺酯,並將其用作納米粒子交聯劑與殼聚醣相互反應形成形狀記憶冷凍凝膠。在形狀記憶過程中,使用原位廣角 X 射線散射(WAXS)和小角度 X 射線散射(SAXS)闡明了熱誘導形狀記憶的機理。原位 WAXS 在形狀固定和恢復的過程中顯示出交聯劑和冷凍凝膠的結晶度變化。原位 SAXS 揭示了交聯劑和冷凍凝膠的結晶位向,這些結晶位向在形狀記憶過程中扮演著舉足輕重的地位。本研究在不同環境下(空氣中或水中)探討形狀記憶冷凍凝膠的恢復行為。在空氣中的形狀記憶測試如下:將條形冷凍凝膠在 50℃下變形為 U 形並在-20℃環境中固定形狀,最後在 50℃的空氣中恢復為條形。在水中的形狀記憶測試如下:於空氣中將條形冷凍凝膠在 50℃下變形為 U 形並在-20℃環境中固定形狀,然後將此 U 形冷凍凝膠壓縮成團並放入 4℃的水中,冷凍凝膠在 4℃的水中預計恢復為 U 形,這意味著冷凍凝膠的彈性恢復能力;在 37℃的水中預計恢復為條型,這代表冷凍凝膠的可切換形狀記憶形狀,而藉由可切換形狀的能力,可以將尺寸較大的 10 毫米 × 10 毫米 × 1.1 毫米的片狀形狀記憶冷凍凝膠,或是複雜結構(如:字母 N、T 或是 U)的形狀記憶冷凍膠固定為棒狀,通過 16 G 針頭擠出並在 37℃的水中恢復為初始形狀。而在此形狀記憶冷凍凝膠支架中生長的人骨髓間充質幹細胞亦表現出長期的增殖能力和形成軟骨的潛能。總結來說,本研究製備了具有大尺寸或複雜結構的可注射形狀記憶可分解殼聚醣-聚胺酯冷凍凝膠,具有良好的細胞相容性,且未來在組織工程與微創手術具有應用潛力。 | zh_TW |
dc.description.abstract | Cryogels are matrices that are formed in moderately frozen solutions of monomeric or polymeric precursors. They have the advantages of interconnected macropores, structural stability, and compressibility. Meanwhile, thermally induced shape memory is an attractive feature of certain functional materials. Although there have been several works concerning shape memory cryogels, little work has been done on shape memory cryogels with biodegradability. In this study, a water-based biodegradable difunctional polyurethane with shape memory property was synthesized and used as the nanoparticulate crosslinker to react with chitosan to form shape memory cryogel. The thermally-induced shape memory mechanism was clarified using in-situ wide-angle X-ray scattering (WAXS) and small-angle X-ray scattering (SAXS)during the shape memory process. The in-situ WAXS showed the changes of crystallinity in the crosslinker and the cryogel during the shape fixation and recovery processes. The in-situ SAXS revealed the orientation of crystallinity of the crosslinker and the cryogel as the mechanism for shape memory. The strip-shape cryogel was deformed at 50℃ to U-shape and fixed at - 20℃, which was squeezable at 25℃ and returned to the strip-shape at 50℃ in air. The shape recovery was further tested in water under two different temperatures. The injected cryogel recovered to the U-shape in 4℃ water, representing elastic recovery, and transformed to the long strip in 37℃ water, representing the switchable shape memory. Moreover, the shape memory cryogel sheet with a large dimension (10 mm × 10 mm × 1.1 mm cryogel sheet) or in complex structure (N, T, or U shapes) could be fixed as a rod, injected through a 16 G needle, and return to their original shapes in 37℃ water, all of which could not be achieved by the conventional cryogel. Human mesenchymal stem cells grown in the shape memory cryogel scaffolds displayed long-term proliferation and chondrogenic potential. The unique injectability and cytocompatibility suggested potential applications of shape memory cryogel as injectable and expandable templates for tissue engineering and minimally invasive surgery. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:29:58Z (GMT). No. of bitstreams: 1 U0001-0203202113261800.pdf: 4535371 bytes, checksum: e37edb199178f690e576b55cf7bece57 (MD5) Previous issue date: 2021 | en |
dc.description.tableofcontents | 致謝 I 摘要 II Abstract IV 目錄 VI 圖目錄 X 表目錄 XII 第一章 文獻回顧 1 1.1. 可注射冷凍凝膠的發展與應用 1 1.2. 形狀記憶材料的發展 2 1.3. 形狀記憶聚胺酯 3 1.4. 可生物降解聚胺酯 4 1.5. 水性聚胺酯 5 1.6. 殼聚醣及其衍伸的生醫材料和應用 5 1.7. 研究目的 6 第二章 研究方法 7 2.1. 研究架構 7 2.2. 聚左旋乳酸二元醇之合成與鑑定 8 2.2.1. 聚左旋乳酸二元醇之合成 8 2.2.2. 聚左旋乳酸二元醇之分子量鑑定 9 2.3. 雙官能基聚胺酯交聯劑之材料製備與分析 10 2.3.1. 雙官能基聚胺酯交聯劑之合成 10 2.3.2. 雙官能基聚胺酯交聯劑膜之製備 11 2.3.4. 納米粒徑和表面電位分析儀 12 2.3.5. 傅立葉紅外光譜儀 13 2.4. 水性N-羧乙基殼聚醣之合成與鑑定 13 2.4.1 水性N-羧乙基殼聚醣之合成 13 2.4.2 水性N-羧乙基殼聚醣之取代度鑑定 14 2.5. 形狀記憶冷凍凝膠之製備 15 2.6. 熱性質分析 16 2.6.1. 熱重分析儀 16 2.6.2. 熱示差掃描分析儀 16 2.7. 形狀記憶測試 17 2.7.1 雙官能基聚胺酯交聯劑及形狀記憶冷凍凝膠於空氣中之形狀記憶測試 17 2.7.2 形狀記憶冷凍凝膠於水中之形狀記憶測試 18 2.8. 微結構分析 19 2.8.1. 廣角度X射線繞射分析儀 19 2.8.2. 原位小角度及廣角度X射線散射儀 19 2.9. 物理和化學性質分析 20 2.9.1. 雙官能基聚胺酯交聯劑及形狀記憶冷凍凝膠之交聯程度測試 20 2.9.2. 形狀記憶冷凍凝膠之體外降解測試 20 2.9.3. 形狀記憶冷凍凝膠之吸水率與吸水時間測試 21 2.9.4. 形狀記憶冷凍凝膠之孔隙度與孔徑大小測試 21 2.9.5. 形狀記憶冷凍凝膠之動態力學分析 22 2.10. 可注射能力測試 22 2.10.1. 大尺寸冷凍凝膠之可注射能力測試 22 2.10.2. 複雜結構冷凍凝膠之可注射能力測試 23 2.11. 人骨髓間充質幹細胞(hMSCs)相容性測試 23 2.11.1. hMSCs螢光染色 23 2.11.2. hMSCs增殖測試 24 2.11.3. hMSCs分化之基因表現測試 24 2.11.4. hMSCs分化之醣胺聚醣(GAG)分析 25 2.12. 統計分析 25 第三章 實驗結果 26 3.1. 雙官能基聚胺酯交聯劑性質之基本測試 26 3.1.1. 聚左旋乳酸二元醇之核磁共振分析 26 3.1.2. 傅立葉紅外線光譜儀 26 3.1.3. 分子量、粒徑和電負度測試 27 3.1.4. 空氣中形狀記憶測試 27 3.2. 冷凍凝膠之形狀記憶測試 28 3.2.1. 空氣中形狀記憶測試 28 3.2.2. 水中形狀記憶測試 29 3.3. 水與氫鍵作用力對形狀記憶之影響 30 3.4. 物化性質測試 30 3.4.1. 熱性質和結晶性質測試 30 3.4.2. 原位廣角度X射線散射測試 31 3.4.3. 原位小角度X射線散射測試 32 3.4.4. 交聯程度測試 33 3.4.5. 形狀記憶冷凍凝膠之體外降解分析 33 3.4.6. 形狀記憶冷凍凝膠之吸水率及吸水時間測試 34 3.4.7. 形狀記憶冷凍凝膠之孔隙度及孔徑大小測試 34 3.4.8. 形狀記憶冷凍凝膠之動態機械性質測試 35 3.5. 形狀記憶冷凍凝膠之可注射測試 35 3.6. 形狀記憶冷凍凝膠之生物相容性測試 36 3.6.1. hMSCs增殖程度測試 36 3.6.2. hMSCs分化程度測試 37 第四章 討論 38 4.1. 可注射冷凍凝膠的限制與發展 38 4.2. 基於雙官能基聚胺酯交聯劑的冷凍凝膠 38 4.3. 形狀記憶冷凍凝膠的最佳化過程 39 4.4. 聚胺酯交聯劑以及冷凍凝膠的形狀記憶機制 39 4.5. 水合狀態下冷凍凝膠的形狀記憶特性 41 4.6. 形狀記憶冷凍凝膠的物理化學特性 42 4.7. 形狀記憶冷凍凝膠的可注射性 43 4.8. 形狀記憶冷凍凝膠的生物相容性 44 4.9. 形狀記憶冷凍凝膠的潛在應用 45 第五章 結論 47 參考文獻 48 圖表 54 | |
dc.language.iso | zh-TW | |
dc.title | 具有可切換形狀記憶特性之殼聚醣-生物可降解聚胺酯冷凍凝膠 | zh_TW |
dc.title | A Biodegradable Chitosan-Polyurethane Cryogel with Switchable Shape Memory | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 莊偉綜(Wei-Tsung Chuang),張書瑋(Shu-Wei Chang),高震宇(Chen-Yu Kao) | |
dc.subject.keyword | 形狀記憶聚胺酯交聯劑,形狀記憶冷凍凝膠,殼聚醣,可注射性,生物 可降解性, | zh_TW |
dc.subject.keyword | cryogel,polyurethane,chitosan,shape memory,injectable, | en |
dc.relation.page | 81 | |
dc.identifier.doi | 10.6342/NTU202100767 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2021-03-16 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
顯示於系所單位: | 高分子科學與工程學研究所 |
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