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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳克強(Ke-Qiang Wu) | |
| dc.contributor.author | YI-SUI HUANG | en |
| dc.contributor.author | 黃一穗 | zh_TW |
| dc.date.accessioned | 2021-06-17T08:25:20Z | - |
| dc.date.available | 2024-08-18 | |
| dc.date.copyright | 2019-08-18 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-08-12 | |
| dc.identifier.citation | Bae, G. and Choi, G. (2008) Decoding of light signals by plant phytochromes and their interacting proteins. Annu Rev Plant Biol. 59, 281-311
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74229 | - |
| dc.description.abstract | 植物的光敏色素會通過調節下游基因的表達來控制多個光相關的生理過程,但關於光敏色素在光相關的基因剪接中扮演什麼作用知之甚少。MOS4-Associated Complex(MAC)是一個會與剪接體結合的高度保守的核蛋白複合物。前人的研究中發現,MAC 的核心亞基 MAC3A、MAC3B 和 MOS4 與組蛋白去乙烯化酶 HDA15能交互作用。HDA15 會參與調控光相關的葉綠素生物合成和下胚軸伸長。我們的研究發現,在黑暗下 MAC3A、MAC3B 和 MOS4 會負調節葉綠素生物合成及在遠紅光下會影響下胚軸的生長。此外,mac3a mac3b 雙突變體中光相關基因 HYH、HFR1、PRR7 和 PAPP5 的剪接在遠紅光下也發生了改變。綜合以上結果,我們的研究發現 MAC3A 和 MAC3B 可能通過調節光相關基因的剪接從而影響遠紅光下下胚軸的生長。 | zh_TW |
| dc.description.abstract | Phytochromes control multiple light-dependent physiological processes by regulating gene expression, but little is known about the roles of phytochromes in
controlling pre-mRNA splicing in response to light. The MOS4-associated complex(MAC) is a highly conserved nuclear protein complex associated with the spliceosome. Previous studies indicate that the core subunits of MAC, MAC3A, MAC3B and MOS4, can interact with the histone deacetylase HDA15 involved in light dependent chlorophyll biosynthesis and hypocotyl elongation. In this study, we found that MAC3A, MAC3B and MOS4 negatively regulate chlorophyll biosynthesis in the dark and hypocotyl elongation under far-red light. In addition, the alternatively spliced transcripts of the light regulated genes, HYH, HFR1, PRR7 and PAPP5, are altered in the mac3a mac3b double mutant under far-red light. Taken together, our studies indicate that MAC3A and MAC3B may play an essential role in far red light regulated hypocotyl elongation through regulating the splicing of light regulated genes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T08:25:20Z (GMT). No. of bitstreams: 1 ntu-108-R05b42029-1.pdf: 2678467 bytes, checksum: 6d3017c1b96eee19ef13beee86aa1277 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 摘要 ii
ABSTRACT iii List of Figures vi List of Supplementary Figures vii List of Supplementary Tables viii Chapter 1 Introduction 1 1.1 Light regulation of plant development 1 1.2 Alternative splicing and light responses 2 1.3 Histone modifications 4 1.4 Histone modifications and alternative splicing 6 1.5 MOS4-ASSOCIATED COMPLEX 7 Chapter 2 Materials and Methods 11 2.1 Plant materials 11 2.2 Generating MAC3A-GFP and MAC3B-GFP transgenic plants 12 2.3 DNA extraction 13 2.4 RNA isolation 14 2.5 DNase treatment 15 2.6 Reverse transcription 15 2.7 Real-time PCR analysis 16 2.8 Protoplast transformation 16 2.9 Protochlorophyllide determination assays 19 2.10 Co-immunoprecipitation assays 20 Chapter 3 Results 22 3.1 Expression and subcellular localization of MAC3A and MAC3B 22 3.2 HDA15 interacted with MAC3A and MAC3B in vivo 22 3.3 Identification of mac3a hda15 and mac3b hda15 double mutants 23 3.4 MAC3A and MAC3B regulate protochlorophyllide biosynthesis 23 3.5 The hypocotyl elongation phenotypes of hda15, mac3a, mac3a3b and hda15mac3a 24 3.6 The hypocotyl phenotype of 35S:MAC3A-GFP/mac3a and 35S:MAC3B-GFP/mac3a3b 25 3.7 Expression patterns of the genes involved in hypocotyl elongation 26 3.8 Alternative Splicing Analysis 26 3.9 Function of MOS4 in photomophogenesis. 28 Chapter4 Discussion 29 4.1 MAC3A, MAC3B and MOS4 participate in photomorphogenesis 29 4.2 The alternative splicing of light related genes are different in mac3a3b mutant under far red light 30 4.3 MAC3A and MAC3B interact with HDA15 30 Figure 32 SUPPLEMENTARY FIGURES 44 SUPPLEMENTARY TABBLES 50 REFERENCES 54 | |
| dc.language.iso | en | |
| dc.subject | 選擇性剪接 | zh_TW |
| dc.subject | 遠紅光 | zh_TW |
| dc.subject | MAC | zh_TW |
| dc.subject | 下胚軸生長 | zh_TW |
| dc.subject | MAC | en |
| dc.subject | far red light | en |
| dc.subject | alternative splicing | en |
| dc.subject | hypocotyl elongation | en |
| dc.title | MAC3A和MAC3B參與在光影響的葉綠素生合成和植物下胚軸生長 | zh_TW |
| dc.title | MAC3A and MAC3B are involved in light dependent chlorophyll biosynthesis and hypocotyl elongation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 謝旭亮(Xu-Liang Xie),靳宗洛(Zong-Luo Jin),鄭貽生(Yi-Sheng Zheng),涂世隆(Shi-Long Tu) | |
| dc.subject.keyword | MAC,遠紅光,選擇性剪接,下胚軸生長, | zh_TW |
| dc.subject.keyword | MAC,far red light,alternative splicing,hypocotyl elongation, | en |
| dc.relation.page | 59 | |
| dc.identifier.doi | 10.6342/NTU201903066 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2019-08-13 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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