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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張麗冠(Li-Kwan Chang) | |
| dc.contributor.author | Bo-Hung Liao | en |
| dc.contributor.author | 廖博弘 | zh_TW |
| dc.date.accessioned | 2021-06-17T08:22:00Z | - |
| dc.date.available | 2024-08-16 | |
| dc.date.copyright | 2019-08-16 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-08-13 | |
| dc.identifier.citation | Adamson, A.L., Kenney, S., 2001. Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies. Journal of Virology 75, 2388-2399.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74151 | - |
| dc.description.abstract | Epstein-BarrVirus(EBV)，屬於皰疹病毒 γ亞科，已被發現和許多腫瘤性癌症有關。 EBV 平時處於潛伏期，當受到誘導或環境刺激時，會進入溶裂期並形成病毒顆粒。 在溶裂期的過程中，轉譯出的外鞘蛋白質會進入細胞核進行外鞘組裝。但在六種外 鞘蛋白質中，卻只有 BORF1、BVRF2 和 BdRF1 具有入核序列，並可以藉此序列 進入細胞核，其餘外鞘蛋白質的入核機制則尚未完全明瞭。在簡單皰疹病毒中，架 構蛋白質具有協助主外鞘蛋白質入核的能力 ; 然而在 EBV 中，免疫螢光染色的結 果發現只有在 BFRF3 共同存在的情形下，VCA 才能藉由 BdRF1 進入細胞核。因 此，本研究欲探究 BFRF3 在 VCA 入核機制中所扮演的角色。首先證實了 VCA 會 與 BFRF3 結合，接著蔗糖濃度梯度分析的結果顯示 BFRF3 會解開 VCA 的多聚體， 並以氨基酸剔除的實驗證實 BFRF3 氨基酸序列 1-65 的區段負責解開 VCA 的聚合 體。最後，本研究也發現 VCA、BdRF1 和 BFRF3 入核後會聚集於核內的 PML- NBs。綜合以上結果，本研究對於 EBV 的外鞘蛋白質如何入核進行外鞘的組裝提 出更深入的機制探討。 | zh_TW |
| dc.description.abstract | Epstein-Barr Virus (EBV) belongs to the gammaherpesvirus family, and is known to be associated with several neoplastic diseases. EBV usually remains latent after infection. Upon stimulated by environmental factors, EBV would enter into the lytic cycle and then produce infective viral particles. During the lytic cycle, capsid proteins need to be translocated into the nucleus for capsid assembly. Among them, only BORF1, BVRF2, and BdRF1 contain the nuclear localization signal (NLS), while the translocation pathways of other capsid proteins are unclear. In herpes simplex virus 1 (HSV-1), scaffold protein is responsible for the translocation of major capsid protein. However, the immunofluorescence results indicated that translocation of VCA is not dependent on BdRF1, unless BFRF3 is also present. Therefore, this study aims to clarify the involvement of BFRF3 in the translocation of VCA. This study found that BFRF3 binds to VCA. Sucrose gradient analysis subsequently revealed that BFRF3 is required for the reduction of VCA oligomerization. Deletion analysis indicates that amino acid residues 1-65 of BFRF3 is required for VCA de-oligomerization. Finally, this study confirms that VCA, BFRF3, and BdRF1 aggregate at promyelocytic leukemia nuclear bodies (PML- NBs). The study provides insight into the mechanism how capsid proteins were translocated into the nucleus for capsid assembly. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T08:22:00Z (GMT). No. of bitstreams: 1 ntu-108-R06b22038-1.pdf: 26248932 bytes, checksum: 6411157e340049c91015c5900c2b476b (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 致謝I
摘要II Abstract III 常用縮寫表 IV 目錄 V 1 前言................1 1.Epstein-Barr virus (EBV) 的發現與特性............................1 1.1 EBV 的發現與相關疾病.....................................1 1.2 EBV 的結構.............................................2 1.3 EBV 的遺傳物質........................................................3 1.4 EBV 的生活史................3 病毒顆粒的組成與形成過程 ...............................................................................5 2.1 皰疹病毒的外鞘結構.......................................................................................5 2.2 EBV 的外鞘結構..............................................7 2.3 皰疹病毒外鞘蛋白質間的作用與自組裝機制...............................................8 2.4 EBV 的小外鞘蛋白質 BFRF3..........................................................................9 2.5 皰疹病毒外鞘的成熟過程.............................................................................11 2.6 EBV 外鞘的成熟過程.....................................................................................12 2.7 皰疹病毒顆粒的生成過程.............................................................................13 外鞘蛋白質的入核 .............................................................................................13 3.1 皰疹病毒的外鞘蛋白質入核路徑.................................................................13 3.2 EBV 的外鞘蛋白質入核路徑.........................................................................14 研究目的 ..............16 材料與方法 ....17 1. 細胞株 .........17 2. 細菌 ......17 3. 質體與抗體 ...18 4. 質體 DNA 的萃取.......18 5. 細胞轉染 .........18 6. GST 融合蛋白質沉降分析 (GST Pull-down) ...................................................18 7. 共同免疫沉降分析 (Co-immunoprecipitation) .................................................19 8. SDS-PAGE 蛋白質膠體電泳及西方點墨法分析 (Western blot analysis).......20 9. 免疫螢光染色分析 (Immunofluorescence analysis).........................................20 10. 蔗糖濃度梯度分析 (Sucrose gradient analysis)..............................................21 結果 ....23 1. VCA 無法藉由 BdRF1 入核 ..............23 2. BFRF3 協助 BdRF1 將 VCA 轉移至宿主細胞細胞核中 ................................24 3. VCA 和 BFRF3 在 in vivo 和 in vitro 的結合....................................................25 4. BFRF3 會改變 VCA 的多聚體型態 ..................................................................26 5. BFRF3 的氨基酸序列 1-65 對 VCA 聚合體型態的影響.................................27 6. BFRF3 透過氨基酸序列 1-88 協助 VCA 入核.................................................27 7. VCA 藉由 BdRF1 於細胞核內再次形成多聚體 ..............................................28 8. 進入細胞核內的外鞘蛋白質會集中於 PML-NBs 區域...................................29 討論 ............................31 1. VCA 和 BFRF3 在細胞中的結合 ......................................................................32 2. 不同種 BFRF3 融合蛋白質對於免疫螢光染色分析的影響 ...........................33 3. 外鞘蛋白質對於 VCA 聚合體型態的影響.......................................................33 4. BFRF3 重要的氨基酸片段可能的生理功能 ....................................................35 5. VCA、BFRF3 和 BdRF1 入核後會集中於 PML-NBs 中................................36 圖表 .....................................38 表 1、本研究使用的質體 .....38 表 2、本研究使用的抗體 ......42 圖 1、EBV 的結構..............43 圖 2、成熟的 EBV 外鞘..............44 圖 3、EBV 外鞘蛋白質在 293T 細胞中的分佈情形..............................................45 圖 4、BdRF1 和 BFRF3 對於 VCA 入核的影響 ....................................................47 圖 5、VCA 會和 BFRF3 結........48 圖 6、BFRF3 和其餘外鞘蛋白質對 VCA 聚合體型態的影響 ..............................51 圖 7、BFRF3 氨基酸片段對 VCA 聚合體型態的影響 ..........................................54 圖 8、BFRF3 的氨基酸片段對 VCA 入核的影響 ..................................................55 圖 9. BdRF1 對核內 VCA 聚合體型態的影響 ........................................................57 圖 10. VCA、BdRF1 及 BFRF3 會共同位在 PML-NBs.........................................59 圖 11. BFRF3 在 VCA 入核路徑中的功能示意圖 .....60 附錄 ..............61 附錄 1、皰疹病毒 HSV-1 的外鞘成熟過程 ............................................................61 附錄 2、EBV 外鞘蛋白質和皰疹病毒同源蛋白質對照表 ....................................63 參考文獻 ..........................................................................................................64 | |
| dc.language.iso | zh-TW | |
| dc.title | EB 病毒的 BFRF3 在 VCA 入核機制中所扮演的角色 | zh_TW |
| dc.title | Role of Epstein-Barr virus BFRF3 in nuclear translocation of VCA | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉世東,羅凱尹,廖憶純,張沛鈞 | |
| dc.subject.keyword | Epstein-Barr virus (EBV),VCA,BdRF1,BFRF3,oligomerization, | zh_TW |
| dc.relation.page | 77 | |
| dc.identifier.doi | 10.6342/NTU201901210 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2019-08-14 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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