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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林長平 | |
dc.contributor.author | Chih-Wei Shih | en |
dc.contributor.author | 施智為 | zh_TW |
dc.date.accessioned | 2021-06-17T08:08:14Z | - |
dc.date.available | 2024-08-20 | |
dc.date.copyright | 2019-08-20 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-17 | |
dc.identifier.citation | 石佳平。2008。枯草桿菌 Bacillus subtilis Y1336 及放線菌 Streptomyces candidus Y21007 菌種之分子鑑定技術及其抑菌效果之研究。碩士論文。高雄:國立高雄師範大學生物科技系。
吳雅芳、鄭安秀。2014。十字花科蔬菜黑腐病菌種子帶菌檢測與去病原技術。行政院農業委員會臺南區農業改良場研究報告,430:43-55. 周浩平、陳以錚。2016。應用液化澱粉芽孢桿菌PMB01防治作物土壤傳播性病害。農政與農情,283:100-102。 謝奉家、李美珍、高穗生。2003。枯草桿菌菌體及其代謝產物對病原真菌之抑菌效果評估。植物保護學會會刊,45:155-162。 Adinarayana, K., and Ellaiah, P. 2002. Response surface optimization of the critical medium components for the production of alkaline protease by a newly isolated Bacillus sp. J. Pharm. Pharm. Sci. 5(3):272-278. Ageitos, J. M., Vallejo, J. A., Sestelo, A. B. F., Poza, M., and Villa, T. G. 2007. Purification and characterization of a milk-clotting protease from Bacillus licheniformis strain USC13. J. Appl. Microbiol. 103:2205-2213. Agrios, G. N. 2005a. chapter one - Introduction. Pages 3-75 in: Plant Pathology (Fifth Edition). G. N. Agrios, ed. Academic Press, San Diego. Agrios, G. N. 2005b. Plant Pathology. 5 ed. Academic Press, San Diego. Barmuta, P., Ferranti, F., Gibiino, G. P., Lewandowski, A., and Schreurs, D. M. M. 2015. Compact behavioral models of nonlinear active devices using response surface methodology. IEEE Trans. Microwave Theory Tech. 63:56-64. Biondi, E., Kuzmanovic, N., Galeone, A., Ladurner, E., Benuzzi, M., Minardi, P., and Bertaccini, A. 2012. Potential of Bacillus amyloliquefaciens strain d747 as control agent against Pseudomonas syringae pv. actinidiae. J. Plant Pathol. 94:S4.58-S54.58. Borriss, R. 2011. Use of plant-associated Bacillus strains as biofertilizers and biocontrol agents in agriculture. Pages 41-76 in: Bacteria in Agrobiology: Plant Growth Responses. D. K. Maheshwari, ed. Springer Berlin Heidelberg, Berlin, Heidelberg. Box, G. E. P., and Wilson, K. B. 1951. On the experimental attainment of optimum conditions. J. R. Stat. Soc. Series B Stat. Methodol. 13:1-45. Box, G. E. P., and Behnken, D. W. 1960. Some new three level designs for the study of quantitative variables. Technometrics 2:455-475. Chen, X.-H., Vater, J., Piel, J., Franke, P., Scholz, R., Schneider, K., Koumoutsi, A., Hitzeroth, G., Grammel, N., Strittmatter, A. W., Gottschalk, G., Süssmuth, R. D., and Borriss, R. 2006. Structural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB 42. J. Bacteriol. 188:4024-4036. Chen, X. H., Scholz, R., Borriss, M., Junge, H., Mögel, G., Kunz, S., and Borriss, R. 2009. Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease. J. Biotechnol. 140:38-44. Chen, X. H., Koumoutsi, A., Scholz, R., Eisenreich, A., Schneider, K., Heinemeyer, I., Morgenstern, B., Voss, B., Hess, W. R., Reva, O., Junge, H., Voigt, B., Jungblut, P. R., Vater, J., Süssmuth, R., Liesegang, H., Strittmatter, A., Gottschalk, G., and Borriss, R. 2007. Comparative analysis of the complete genome sequence of the plant growth–promoting bacterium Bacillus amyloliquefaciens FZB42. Nat. Biotechnol. 25:1007-1014. Compant, S., Duffy, B., Nowak, J., Clément, C., and Barka, E. A. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl. Environ. Microbiol. 71:4951-4959. Cruz Ramos, H., Hoffmann, T., Marino, M., Nedjari, H., Presecan-Siedel, E., Dreesen, O., Glaser, P., and Jahn, D. 2000. Fermentative metabolism of Bacillus subtilis physiology and regulation of gene expression. J. Bacteriol. 182:3072-3080. D'Este, M., Alvarado-Morales, M., and Angelidaki, I. 2018. Amino acids production focusing on fermentation technologies – A review. Biotechnol. Adv. 36:14-25. Detsch, C., and Stülke, J. 2003. Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB. Microbiology 149:3289-3297. Fisher, R. A. 1992. The arrangement of field experiments. Pages 82-91 in: Breakthroughs in Statistics: Methodology and Distribution. S. Kotz and N. L. Johnson, eds. Springer New York, New York, NY. Franco-Correa, M., Quintana, A., Duque, C., Suarez, C., Rodríguez, M. X., and Barea, J.-M. 2010. Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Appl. Soil Ecol. 45:209-217. Franken, A. A. J. M. 1992. Immunofluorescence microscopy and dilution-plating for the detection of Xanthomonas campestris pv. campestris in crucifer seeds : methods to determine seed health and seed infection. Franken (PhD Thesis). Gómez-Galera, S., Twyman, R. M., Sparrow, P. A. C., Van Droogenbroeck, B., Custers, R., Capell, T., and Christou, P. 2012. Field trials and tribulations—making sense of the regulations for experimental field trials of transgenic crops in Europe. Plant Biotechnol. J. 10:511-523. Gonzalez, R., Islas, L., Obregon, A. M., Escalante, L., and Sanchez, S. 1995. Gentamicin formation in Micromonospora purpurea: stimulatory effect of ammonium. J. Antibiot. 48:479-483. Hayward, A. C. 1993. The hosts of Xanthomonas. Pages 1-119 in: Xanthomonas. J. G. Swings and E. L. Civerolo, eds. Springer Netherlands, Dordrecht. Huang, C. N., Lin, C. P., Hsieh, F. C., Lee, S. K., Cheng, K. C., and Liu, C. T. 2016. Characterization and evaluation of Bacillus amyloliquefaciens strain WF02 regarding its biocontrol activities and genetic responses against bacterial wilt in two different resistant tomato cultivars. World J. Microbiol. Biotech. 32:183. Huang, H. C., Huang, J., Saindon, G., and Erickson, R. S. 1997. Effect of allyl alcohol and fermented agricultural wastes on carpogenic germination of sclerotia of Sclerotinia sclerotiorum and colonization by Trichoderma spp. Can. J. Plant Pathol. 19:43-46. Jacques, P., Hbid, C., Destain, J., Razafindralambo, H., Paquot, M., De Pauw, E., and Thonart, P. 1999. Optimization of biosurfactant lipopeptide production from Bacillus subtilis S499 by Plackett-BurmandDesign. Pages 223-233 in: Twentieth Symposium on Biotechnology for Fuels and Chemicals: Presented as Volumes 77–79 of Applied Biochemistry and Biotechnology Proceedings of the Twentieth Symposium on Biotechnology for Fuels and Chemicals Held May 3–7, 1998, Gatlinburg, Tennessee. B. H. Davison and M. Finkelstein, eds. Humana Press, Totowa, NJ. Kaiser, S. C., Werner, S., Jossen, V., Kraume, M., and Eibl, D. 2017. Development of a method for reliable power input measurements in conventional and single-use stirred bioreactors at laboratory scale. Eng. Life Sci. 17:500-511. Kennedy, M., and Krouse, D. 1999. Strategies for improving fermentation medium performance: a review. Journal of Industrial Microbiology and Biotechnology 23:456-475. Kloepper, J. W., and Schroth, M. N. 1978. Plant growth-promoting rhizobacteria on radishes. Pages 879-882 in: Proceedings of the 4th international conference on plant pathogenic bacteria. Gilbert-Clarey, Tours. Liu, B.-L., and Tzeng, Y.-M. 1998. Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology. Bioprocess Eng. 18:413-418. Liu, Y.-P., Zheng, P., Sun, Z.-H., Ni, Y., Dong, J.-J., and Zhu, L.-L. 2008. Economical succinic acid production from cane molasses by Actinobacillus succinogenes. Bioresour. Technol. 99:1736-1742. Liu, Y. Y. 2018. Evaluation of the multifunctional effects of Bacillus amyloliquefaciens strain WF02 formulated with a phytogenic additive Bidens pilosa against bacterial fruit blotch of melon. National Taiwan University, Taiwan. Lugtenberg, B., and Kamilova, F. 2009. Plant-growth-promoting rhizobacteria. Annu. Rev. Microbiol. 63:541-556. Luna, C. L., Mariano, R. L. R., and Souto-Maior, A. M. 2002. Production of a biocontrol agent for crucifers black rot disease. Braz. J. Chem. Eng. 19:133-140. Martínez-Viveros, O., Jorquera, M. A., Crowley, D. E., Gajardo, G., and Mora, M. L. 2010. Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria. Soil Sci. Plant Nutr. 10:293-319. Massomo, S. M. S., Mortensen, C. N., Mabagala, R. B., Newman, M. A., and Hockenhull, J. 2004. Biological control of black rot (Xanthomonas campestris pv. campestris) of cabbage in Tanzania with Bacillus strains. J. Phytopathol. 152:98-105. Pérez-García, A., Romero, D., and de Vicente, A. 2011. Plant protection and growth stimulation by microorganisms: biotechnological applications of Bacilli in agriculture. Curr. Opin. Biotechnol. 22:187-193. Panda, T., Bisaria, V. S., and Ghose, T. K. 1983. Studies on mixed fungal culture for cellulase and hemi-cellulase production part-1: Optimization of medium for the mixed culture of Trichoderma reesei D1-6 and Aspergillus Pt 2804. Biotechnol. Lett. 5:767-772. Patel, G. B., Khan, A. W., and Roth, L. A. 1978. Optimum levels of sulphate and iron for the cultivation of pure cultures of methanogens in synthetic media. J. Appl. Bacteriol. 45:347-356. Priest, F. G., Goodfellow, M., Shute, L. A., and Berkeley, R. C. W. 1987. Bacillus amyloliquefaciens sp. nov., nom. rev. Int. J. Syst. Evol. Microbiol. 37:69-71. Ryu, J. H., Kim, H., and Beuchat, L. R. 2005. Spore formation by Bacillus cereus in broth as affected by temperature, nutrient availability, and manganese. J. Food Prot. 68(8):1734-1738. Sansinenea, E., and Ortiz, A. 2011. Secondary metabolites of soil Bacillus spp. Biotechnol. Lett. 33:1523-1538. Schallmey, M., Singh, A., and Ward, O. P. 2004. Developments in the use of Bacillus species for industrial production. Can. J. Microbiol. 50:1-17. Scholz, R., Molohon, K. J., Nachtigall, J., Vater, J., Markley, A. L., Süssmuth, R. D., Mitchell, D. A., and Borriss, R. 2011. Plantazolicin, a novel microcin B17/Streptolysin S-like natural product from Bacillus amyloliquefaciens FZB42. J. Bacteriol. 193:215-224. Shafi, J., Tian, H., and Ji, M. 2017. Bacillus species as versatile weapons for plant pathogens: a review. Biotechnol. Biotechnol. Equip. 31:446-459. Shaker, H. M., Farid, M. A., and El-Diwany, A. I. 1984. Optimization of the composition of the nutrient medium for cellulase and protein biosynthesis by thermophilic Aspergillus fumigatus NRC 272. Enzyme Microb. Technol. 6:212-216. Shaw, J. J., and Kado, C. I. 1988. Whole plant wound inoculation for consistent reproduction of black rot of crucifers. Phytopathology 78(7):981-986. Silveira, R. G., Kakizono, T., Takemoto, S., Nishio, N., and Nagai, S. 1991. Medium optimization by an orthogonal array design for the growth of Methanosarcina barkeri. J. Ferment. Bioeng. 72:20-25. Sinclair, J. B., and Dhingra, O. D. 1995. Basic Plant Pathology Methods. Taylor & Francis. Singh, V., Haque, S., Niwas, R., Srivastava, A., Pasupuleti, M., and Tripathi, C. K. M. 2017. Strategies for fermentation medium optimization: an in-depth review. Front. Microbiol. 7:2087. Swings, J., and Civetta, L. 2012. Xanthomonas. Springer Science & Business Media, Berlin. Tanyildizi, M. S., Özer, D., and Elibol, M. 2005. Optimization of α-amylase production by Bacillus sp. using response surface methodology. Process Biochem. 40:2291-2296. Tisdale, S. L., Nelson, W. L., and Beaton, J. D. 1985. Soil Fertility and Fertilizers. Collier Macmillan Publishers, London. Vasantha, N., and Freese, E. 1979. The role of manganese in growth and sporulation of Bacillus subtilis. Microbiology 112:329-336. Vicente, J. G., and Holub, E. B. 2013. Xanthomonas campestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops. Mol. Plant Pathol. 14:2-18. Wang, E. T., and Martínez-Romero, E. 2000. Sesbania herbacea–Rhizobium huautlense nodulation in flooded soils and comparative characterization of S. herbacea-nodulating rhizobia in different environments. Microb. Ecol. 40:25-32. Williams, P. H. 1980. Black rot: a continuing threat to world crucifers. Plant Dis. 64:736-742. Ye, R. W., Tao, W., Bedzyk, L., Young, T., Chen, M., and Li, L. 2000. Global gene expression profiles of Bacillus subtilis grown under anaerobic conditions. J. Bacteriol. 182:4458. Yuan, J., Raza, W., Shen, Q., and Huang, Q. 2012. Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense. Appl. Environ. Microbiol. 78:5942-5944. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73696 | - |
dc.description.abstract | 液化澱粉芽孢桿菌WF02菌株分離自台中霧峰山區,已被證明具有防治Ralstonia solanacearum所引起之番茄細菌性青枯病以及Acidovorax avenae subsp. citrulli 所引起之瓜類細菌性果斑病的功效。在前期試驗發現WF02對於病原菌的拮抗能力與其活菌數呈正相關,因此本研究欲開發出能符合經濟效益,且在最短單位時間內可獲得最大生物量的培養基組成與發酵條件。初期利用三角搖瓶培養系統進行培養基組成與菌體生長條件的因子探討,篩選出糖蜜與硝酸銨作為理想的碳氮源組成,pH值的範圍則介於7~9之間,最適培養溫度則是落在37℃。使用桌上型全自動控制發酵槽針對pH、溶氧(DO)、攪拌速率等參數進行調節控制,以回應曲面法數學統計模型進行最佳發酵條件預測,藉由因子交互作用分析、因子交互作用最佳化等試驗使WF02能迅速達到生長增殖高峰。實驗結果發現WF02的生物量與糖蜜濃度、硝酸銨濃度、pH值間的關係與二階模型吻合,在最適條件下WF02活菌數可達109 cfu/ml以上。使用該培養基組成與發酵條件約提升了60%的WF02生物量,生產成本也降低了90%。此外,相較於先前試驗所使用的LB發酵產物,此優化發酵產物在對抗十字花科黑腐病之生物防治效率亦提升了17%。未來將結合本研究所建立之發酵技術與製劑化開發,提升菌株的儲架壽命與防治效果,將有助於WF02的商品化。 | zh_TW |
dc.description.abstract | Bacillus amyloliquefaciens strain WF02 was isolated from Wufeng mountain, Taichung city. It has been demonstrated that WF02 could control tomato bacteria wilt caused by Ralstonia solanacearum, as well as control bacterial fruit blotch caused by Acidovorax avenae subsp. citrulli. In my preliminary data indicated that the antagonistic activity of this bacterium was positively correlated with its biomass. Accordingly, the purpose of this study was to develop the optimal fermentation conditions that are economically viable, and that yield maximum biomass in the shortest unit of time. In the initial stage of optimization, the shake-flask culture system was used to investigate the factors associated with cell culture conditions. The molasses and ammonium nitrate were selected as the ideal carbon and nitrogen sources. The pH level ranged from 7 to 9, and the optimum culture temperature was 37°C. A desktop bioreactor was used to control the environment and culture parameters, including pH/ dissolved oxygen (DO)/ stirring speed etc. during fermentation. Then, I employed response surface methodology (RSM) to design the experiments for predicting optimal fermentation conditions. Optimize the WF02 growth to the maximum point with the variables interaction analysis and variables regression. In the results, I found that the relationship between biomass and the parameters, such as concentrations of molasses and ammonium nitrate, and pH were fitting a second-order model. Under the optimum conditions of fermentation, the maximum biomass productivity could be above 109 cfu/ml. The use of this medium composition and fermentation conditions increased the biomass of WF02 by approximately 60%, and the medium cost was also reduced by 90% in comparison with that used previously. Furthermore, the biocontrol effectiveness of the optimized cultural medium against black rot of Chinese cabbage caused by Xcc425 was enhanced (17% increased) in comparison with that of LB cultural medium. In the future, we would like to combine the fermentation technology established by this study with the formulation development to improve the shelf life and biocontrol efficacy for the commercialization of WF02 biocontrol agent. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:08:14Z (GMT). No. of bitstreams: 1 ntu-108-R06633013-1.pdf: 2809552 bytes, checksum: 24add0d6e8a601bda902219490adb732 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 致謝 i
中文摘要 ii ABSTRACT iii Contents v List of Tables vii List of Figures viii Introduction 1 Role of beneficial microorganisms for biocontrol of plant diseases 1 Bacillus as PGPR in crop ecosystem 2 B. amyloliquefaciens as biocontrol agent 2 Black rot of crucifers 4 Optimization of fermentation medium 5 Aim of this study 7 Materials and Methods 8 Bacterial strain, culture media and growth conditions 8 in vitro antagonistic ability of biocontrol agents 9 Preparation of plant material 10 in vivo biocontrol activity of WF02 10 Lab scale fermentation by benchtop bioreactor 11 Response surface methodology (RSM) for statistical optimization of fermentation conditions 11 RESULTS 13 Antagonistic activity of WF02 broth against Xcc425 was related to its biomass production 13 Effects of culture media on the growth rate and antagonistic activity of WF02 14 Select appropriate components for optimization of Landy medium 15 Screening suitable fermentation conditions 17 Response surface methodology 19 Antagonistic assay against Xcc425 by the optimal medium of WF02 22 Discussion 23 Summary 26 Tables and Figures 27 Supplementary Tables and Figures 53 References 59 | |
dc.language.iso | en | |
dc.title | 液化澱粉芽孢桿菌WF02菌株之培養條件優化與生物防治效力評估 | zh_TW |
dc.title | Optimization of culture conditions for production of Bacillus amyloliquefaciens strain WF02 and evaluation of its biocontrol efficacy | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 劉?德 | |
dc.contributor.oralexamcommittee | 劉?睿,李昆達 | |
dc.subject.keyword | 生物防治,液化澱粉芽孢桿菌,培養條件優化,回應曲面法,十字花科黑腐病, | zh_TW |
dc.subject.keyword | biocontrol,Bacillus amyloliquefaciens,optimization of culture conditions,response surface methodology (RSM),black rot of crucifers, | en |
dc.relation.page | 66 | |
dc.identifier.doi | 10.6342/NTU201903912 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-18 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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